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The map label for this gene is pycA [H]

Identifier: 78356584

GI number: 78356584

Start: 1559119

End: 1560534

Strand: Direct

Name: pycA [H]

Synonym: Dde_1541

Alternate gene names: 78356584

Gene position: 1559119-1560534 (Clockwise)

Preceding gene: 78356583

Following gene: 78356585

Centisome position: 41.8

GC content: 56.78

Gene sequence:

>1416_bases
GTGCAGAATACCGAACATAAGGTGCTGGTGGCGAATCGAGGGGAAATCGCCATCCGTATCGTTCAGGCCTGCCGCAAGCT
GGGTCTGGATTTTGTATGTGTATACACGGCGGAAGACGCTGAGTCGGGGCATGTACGCATCGCCCGTCAGCTTGGCGGCG
AAAAAAGCCTGTTCCGTGTATCGTCATATCATGATGCGAACGAACTGTTGTCCGTAGCAGACGAGGCCGGCGCCACCGCC
GTGCATCCCGGCTACGGCTTTTTTGCCGAAGACTACAGGTTCGCGCGGCGGGTATCGCAGCGCGGGCGCAAACTGATATT
CATAGGTCCGTCATGGCGGGTTATCCGCGAGCTGGGTGACAAGATAAATACCAAACGTCTTGCGCGCAGTCTCGGCGTGC
CCACTGTGCCCGGTTCAGACAAACCCATCTACGACGAGATGGAAGCCGAAAAAGTGGCCCAGTCGCTTTTCGAGTTTCAG
GAAGAGCAGGGCGTCGAGCGCCCTCTGGTTCTGGTCAAGGCCTCCGCCGGAGGCGGCGGAATGGGTATAGAGGAAGTGCA
CAGCCTTGATCTGTTCCGCTCCGTGTATCGCCGTATCCGCAATTACGCCCTGCGTCAGTTCAATGACGAGGGTGTTCTTA
TCGAGCAGCGTGTCAACGACTTCAACCATCTGGAAGTTCAGATCGTTTCTGACAGAAGCGGCAAAAATCCCGTGCATTTC
GGCACGAGAAACTGCTCCATACAGTCCACCGGTCTGCAAAAGCGCGTGGAAATTGCTCCGGGGTTCGACTCTTCGTCGTA
TTCCTATGCGTTTGACGCAGACAAACTGCTGGAAGACATCACCAACCACTCTCTGGCTATGGCCCGCAAGGTCGGCTACG
ACAATGTGGGCACATGGGAGTGGATTGTCTCGCGCGACGGGCGTCCCTTTCTGATGGAGGTGAACACCCGCATTCAGGTG
GAAAACGGCGTTTCTGCCGCCATAGCGCGCATTCACGGTAAAGGCGGTGTCGACATAATCGCCGAGCAGATCCGTGTGGG
GCTGGGCGAGCCGCTGGGTTACACGCAGGAAGATATCACATTCGAAGGTGTGGGCATTGAATACCGCATCATCGCGGAAG
ATCCTGACAACCGTTTCACCCCCTGGGTGGGACGTATAGAAGGCTTCCGCTGGGATGAACGTGACTGGCTTTCCATGCAT
ACGCATGTGCCCACTGACAGTCCGTATGACATTCCCACTGAGTTCGACCCCAACCTTGCTCTTGCCATTGTGTGGGGCAA
AGACCTTGCACAGGTCCGGGAGCGGGGGCTGGAACTGCTGGACACCATCACGCTGGAGGGCGTTAATCAGGCCGGTCCCA
TGCGCACCAACATTGCTTTTCTGCGCAGAAAGACGGCTGATGTTCTCCGTTTCTGA

Upstream 100 bases:

>100_bases
TGGACAAGCTTATTCTGGAAGTGCAGAAACGGCAGATCGCCATGAAAAGGGCGACTGTGTGTGATGAAGAACTGGAAAAA
TAAACAGTCGAGGGACATTG

Downstream 100 bases:

>100_bases
TGTGTCGGGTGCGGCCGTTTTTGTCCGTTATTCTGCGGTACAGAGGCGGCTGTTCTTTTCCGGCAGGTATTCCGGTGTGT
AGCCGCCGCGCGGTTGCCCG

Product: pyruvate carboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MQNTEHKVLVANRGEIAIRIVQACRKLGLDFVCVYTAEDAESGHVRIARQLGGEKSLFRVSSYHDANELLSVADEAGATA
VHPGYGFFAEDYRFARRVSQRGRKLIFIGPSWRVIRELGDKINTKRLARSLGVPTVPGSDKPIYDEMEAEKVAQSLFEFQ
EEQGVERPLVLVKASAGGGGMGIEEVHSLDLFRSVYRRIRNYALRQFNDEGVLIEQRVNDFNHLEVQIVSDRSGKNPVHF
GTRNCSIQSTGLQKRVEIAPGFDSSSYSYAFDADKLLEDITNHSLAMARKVGYDNVGTWEWIVSRDGRPFLMEVNTRIQV
ENGVSAAIARIHGKGGVDIIAEQIRVGLGEPLGYTQEDITFEGVGIEYRIIAEDPDNRFTPWVGRIEGFRWDERDWLSMH
THVPTDSPYDIPTEFDPNLALAIVWGKDLAQVRERGLELLDTITLEGVNQAGPMRTNIAFLRRKTADVLRF

Sequences:

>Translated_471_residues
MQNTEHKVLVANRGEIAIRIVQACRKLGLDFVCVYTAEDAESGHVRIARQLGGEKSLFRVSSYHDANELLSVADEAGATA
VHPGYGFFAEDYRFARRVSQRGRKLIFIGPSWRVIRELGDKINTKRLARSLGVPTVPGSDKPIYDEMEAEKVAQSLFEFQ
EEQGVERPLVLVKASAGGGGMGIEEVHSLDLFRSVYRRIRNYALRQFNDEGVLIEQRVNDFNHLEVQIVSDRSGKNPVHF
GTRNCSIQSTGLQKRVEIAPGFDSSSYSYAFDADKLLEDITNHSLAMARKVGYDNVGTWEWIVSRDGRPFLMEVNTRIQV
ENGVSAAIARIHGKGGVDIIAEQIRVGLGEPLGYTQEDITFEGVGIEYRIIAEDPDNRFTPWVGRIEGFRWDERDWLSMH
THVPTDSPYDIPTEFDPNLALAIVWGKDLAQVRERGLELLDTITLEGVNQAGPMRTNIAFLRRKTADVLRF
>Mature_471_residues
MQNTEHKVLVANRGEIAIRIVQACRKLGLDFVCVYTAEDAESGHVRIARQLGGEKSLFRVSSYHDANELLSVADEAGATA
VHPGYGFFAEDYRFARRVSQRGRKLIFIGPSWRVIRELGDKINTKRLARSLGVPTVPGSDKPIYDEMEAEKVAQSLFEFQ
EEQGVERPLVLVKASAGGGGMGIEEVHSLDLFRSVYRRIRNYALRQFNDEGVLIEQRVNDFNHLEVQIVSDRSGKNPVHF
GTRNCSIQSTGLQKRVEIAPGFDSSSYSYAFDADKLLEDITNHSLAMARKVGYDNVGTWEWIVSRDGRPFLMEVNTRIQV
ENGVSAAIARIHGKGGVDIIAEQIRVGLGEPLGYTQEDITFEGVGIEYRIIAEDPDNRFTPWVGRIEGFRWDERDWLSMH
THVPTDSPYDIPTEFDPNLALAIVWGKDLAQVRERGLELLDTITLEGVNQAGPMRTNIAFLRRKTADVLRF

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=467, Percent_Identity=36.6167023554604, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI106049295, Length=467, Percent_Identity=36.6167023554604, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI106049292, Length=467, Percent_Identity=36.6167023554604, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI65506442, Length=469, Percent_Identity=33.9019189765458, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI295821183, Length=469, Percent_Identity=33.9019189765458, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI189095269, Length=468, Percent_Identity=33.974358974359, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI116805327, Length=466, Percent_Identity=33.2618025751073, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI38679974, Length=446, Percent_Identity=27.3542600896861, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI38679971, Length=446, Percent_Identity=27.3542600896861, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI38679960, Length=446, Percent_Identity=27.3542600896861, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI38679977, Length=447, Percent_Identity=27.7404921700224, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI38679967, Length=447, Percent_Identity=27.7404921700224, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI134142062, Length=446, Percent_Identity=26.6816143497758, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789654, Length=442, Percent_Identity=31.6742081447964, Blast_Score=208, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17567343, Length=465, Percent_Identity=32.6881720430108, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI17562816, Length=464, Percent_Identity=34.698275862069, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI71987519, Length=462, Percent_Identity=32.9004329004329, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI133931226, Length=421, Percent_Identity=25.1781472684086, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71997168, Length=439, Percent_Identity=25.0569476082005, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71997163, Length=439, Percent_Identity=25.0569476082005, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6319685, Length=456, Percent_Identity=32.2368421052632, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6319695, Length=471, Percent_Identity=31.8471337579618, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321376, Length=392, Percent_Identity=35.2040816326531, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324343, Length=451, Percent_Identity=27.4944567627494, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323863, Length=412, Percent_Identity=28.3980582524272, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281363050, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652224, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652222, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652220, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652218, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652212, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652210, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652214, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI19921944, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24652216, Length=465, Percent_Identity=36.3440860215054, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24651757, Length=458, Percent_Identity=31.6593886462882, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24651759, Length=404, Percent_Identity=31.1881188118812, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24586458, Length=507, Percent_Identity=26.6272189349112, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI161076407, Length=507, Percent_Identity=26.6272189349112, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24586460, Length=507, Percent_Identity=26.6272189349112, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI161076409, Length=507, Percent_Identity=26.6272189349112, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52953; Mature: 52953

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50975 ATP_GRASP ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNTEHKVLVANRGEIAIRIVQACRKLGLDFVCVYTAEDAESGHVRIARQLGGEKSLFRV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHCCCCCCEEEE
SSYHDANELLSVADEAGATAVHPGYGFFAEDYRFARRVSQRGRKLIFIGPSWRVIRELGD
CCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
KINTKRLARSLGVPTVPGSDKPIYDEMEAEKVAQSLFEFQEEQGVERPLVLVKASAGGGG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
MGIEEVHSLDLFRSVYRRIRNYALRQFNDEGVLIEQRVNDFNHLEVQIVSDRSGKNPVHF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEE
GTRNCSIQSTGLQKRVEIAPGFDSSSYSYAFDADKLLEDITNHSLAMARKVGYDNVGTWE
CCCCCEEECCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHCHHHHHHHHCCCCCCCCEE
WIVSRDGRPFLMEVNTRIQVENGVSAAIARIHGKGGVDIIAEQIRVGLGEPLGYTQEDIT
EEEECCCCEEEEEECCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCE
FEGVGIEYRIIAEDPDNRFTPWVGRIEGFRWDERDWLSMHTHVPTDSPYDIPTEFDPNLA
EEECCEEEEEEEECCCCCCCCCHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCEE
LAIVWGKDLAQVRERGLELLDTITLEGVNQAGPMRTNIAFLRRKTADVLRF
EEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNTEHKVLVANRGEIAIRIVQACRKLGLDFVCVYTAEDAESGHVRIARQLGGEKSLFRV
CCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHCCCCCCEEEE
SSYHDANELLSVADEAGATAVHPGYGFFAEDYRFARRVSQRGRKLIFIGPSWRVIRELGD
CCCCCHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHH
KINTKRLARSLGVPTVPGSDKPIYDEMEAEKVAQSLFEFQEEQGVERPLVLVKASAGGGG
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCC
MGIEEVHSLDLFRSVYRRIRNYALRQFNDEGVLIEQRVNDFNHLEVQIVSDRSGKNPVHF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEECCCCCCCEEE
GTRNCSIQSTGLQKRVEIAPGFDSSSYSYAFDADKLLEDITNHSLAMARKVGYDNVGTWE
CCCCCEEECCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHCHHHHHHHHCCCCCCCCEE
WIVSRDGRPFLMEVNTRIQVENGVSAAIARIHGKGGVDIIAEQIRVGLGEPLGYTQEDIT
EEEECCCCEEEEEECCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCE
FEGVGIEYRIIAEDPDNRFTPWVGRIEGFRWDERDWLSMHTHVPTDSPYDIPTEFDPNLA
EEECCEEEEEEEECCCCCCCCCHHCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCCCEE
LAIVWGKDLAQVRERGLELLDTITLEGVNQAGPMRTNIAFLRRKTADVLRF
EEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]