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The map label for this gene is metK

Identifier: 78356541

GI number: 78356541

Start: 1516320

End: 1517495

Strand: Reverse

Name: metK

Synonym: Dde_1496

Alternate gene names: 78356541

Gene position: 1517495-1516320 (Counterclockwise)

Preceding gene: 78356542

Following gene: 78356535

Centisome position: 40.68

GC content: 57.4

Gene sequence:

>1176_bases
ATGATCCCTTATAAGGGTAAATACCACTTCACTTCCGAATCGGTTACCGAAGGCCATCCCGACAAAGTCGCTGACCAGAT
TTCCGACGCCGTGCTTGATGTGCTGCTCGAACAGGATCCTGATTCGCGCGTGGCCTGTGAGACCATGGTCACCACCGGCA
TGGCCATCATCGCCGGCGAAATAACCACCAGAGGCTATGCCGACCTGCCGCAGGTGGTGCGGGATACCATCAAAGAAATC
GGATACAATAACTCCTCCATGGGATTTGACTGGCAGACATGCGCCGTGCTGTCTTCCATAGACAAACAGTCTCCTGACAT
TGCTCAGGGCGTTGACCGCCAGGACCCCGAAAATCAGGGCGCCGGTGACCAGGGCATGATGTTCGGTTTTGCCTGCGACG
AAACCCCCACGCTGATGCCCGCCCCCATATTCTGGGCACACCAGCTTTCGCAAAAGCTTACCGCTGTGCGCAAAGACGGC
ACCGTGGATTTTCTGCGCCCCGACGGCAAAACACAGGTGTCCTTTGAATACGTTGACGGCCGCCCGGTACGCATCAACAA
TGTGGTTGTTTCCACACAGCATGCGGAACAGGCCAGCCAGACAGACATCATAGACGCCATCAGAACCCACGTCATCCGCC
CCGTTCTGGAACCTTCCGGCTTCTTCAACGAAAAAGACTGCGAAATTTTCATCAACACCACCGGCCGTTTTGTTATCGGC
GGCCCCATGGGTGACTGCGGTCTTACCGGCCGTAAAATCATTCAGGACACTTACGGCGGCATGGGCAGTCACGGCGGCGG
CGCATTCTCCGGCAAAGACCCCTCCAAAGTGGACCGCTCCGGCGCATACATGGGCCGCTACATTGCCAAAAACGTTGTGG
CAGCCGGACTTGCTCCCAAATGCGAAGTACAGATTGCGTACTGCATCGGCGTGGCCGATCCTGTTTCCGTACTGTGCACC
TCTCTGGGCAGCAGCGACATTTCCGACGAGGTGCTCACTCAGGCCGTGCGCGATGTGTTCGACCTGCGCCCGTACCATAT
CTCCAAACGCCTTGACCTGCGCCGGCCGATCTATAAAAAATCAGCCTGCTACGGACACTTCGGTCGCGAGATTCCCGAAT
TTTCGTGGGAACGCACCGACGCTGTGGACGATCTGCGTGCAGCGGCCCGCCGCTAG

Upstream 100 bases:

>100_bases
CTTATTTCCGGCGGAATTCTGAGTGCAGAGCACGAAAAGTCGAAAAACAGAAAAAAGAAAACAGCCATCAAGGCGAAGAA
CACATCCCAGGAGGAAACGC

Downstream 100 bases:

>100_bases
CCCTATCCCGCCCGTCAGCTTATTCCGGCCGGAGCTTCTTGCTCCGGCTTTTTTTAGGCCCGTATCCGGACACCACAAAC
AAAAGGCGCATCCGCCACAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIPYKGKYHFTSESVTEGHPDKVADQISDAVLDVLLEQDPDSRVACETMVTTGMAIIAGEITTRGYADLPQVVRDTIKEI
GYNNSSMGFDWQTCAVLSSIDKQSPDIAQGVDRQDPENQGAGDQGMMFGFACDETPTLMPAPIFWAHQLSQKLTAVRKDG
TVDFLRPDGKTQVSFEYVDGRPVRINNVVVSTQHAEQASQTDIIDAIRTHVIRPVLEPSGFFNEKDCEIFINTTGRFVIG
GPMGDCGLTGRKIIQDTYGGMGSHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPKCEVQIAYCIGVADPVSVLCT
SLGSSDISDEVLTQAVRDVFDLRPYHISKRLDLRRPIYKKSACYGHFGREIPEFSWERTDAVDDLRAAARR

Sequences:

>Translated_391_residues
MIPYKGKYHFTSESVTEGHPDKVADQISDAVLDVLLEQDPDSRVACETMVTTGMAIIAGEITTRGYADLPQVVRDTIKEI
GYNNSSMGFDWQTCAVLSSIDKQSPDIAQGVDRQDPENQGAGDQGMMFGFACDETPTLMPAPIFWAHQLSQKLTAVRKDG
TVDFLRPDGKTQVSFEYVDGRPVRINNVVVSTQHAEQASQTDIIDAIRTHVIRPVLEPSGFFNEKDCEIFINTTGRFVIG
GPMGDCGLTGRKIIQDTYGGMGSHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPKCEVQIAYCIGVADPVSVLCT
SLGSSDISDEVLTQAVRDVFDLRPYHISKRLDLRRPIYKKSACYGHFGREIPEFSWERTDAVDDLRAAARR
>Mature_391_residues
MIPYKGKYHFTSESVTEGHPDKVADQISDAVLDVLLEQDPDSRVACETMVTTGMAIIAGEITTRGYADLPQVVRDTIKEI
GYNNSSMGFDWQTCAVLSSIDKQSPDIAQGVDRQDPENQGAGDQGMMFGFACDETPTLMPAPIFWAHQLSQKLTAVRKDG
TVDFLRPDGKTQVSFEYVDGRPVRINNVVVSTQHAEQASQTDIIDAIRTHVIRPVLEPSGFFNEKDCEIFINTTGRFVIG
GPMGDCGLTGRKIIQDTYGGMGSHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPKCEVQIAYCIGVADPVSVLCT
SLGSSDISDEVLTQAVRDVFDLRPYHISKRLDLRRPIYKKSACYGHFGREIPEFSWERTDAVDDLRAAARR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=379, Percent_Identity=53.0343007915567, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4557737, Length=379, Percent_Identity=52.7704485488127, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=60.9375, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17538498, Length=377, Percent_Identity=52.5198938992042, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=51.1811023622047, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17538494, Length=377, Percent_Identity=51.7241379310345, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25145633, Length=394, Percent_Identity=50.761421319797, Blast_Score=370, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=46.684350132626, Blast_Score=310, Evalue=6e-85,
Organism=Caenorhabditis elegans, GI32565911, Length=330, Percent_Identity=50, Blast_Score=306, Evalue=9e-84,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=48.1159420289855, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=49.1749174917492, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=85, Percent_Identity=57.6470588235294, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320710, Length=376, Percent_Identity=52.9255319148936, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323209, Length=378, Percent_Identity=52.9100529100529, Blast_Score=378, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=49.3438320209974, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=49.3438320209974, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=49.6062992125984, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580541, Length=368, Percent_Identity=48.3695652173913, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=55.9322033898305, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_DESDG (Q311V1)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387990.1
- ProteinModelPortal:   Q311V1
- SMR:   Q311V1
- STRING:   Q311V1
- GeneID:   3756049
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1496
- NMPDR:   fig|207559.3.peg.703
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   DDES207559:DDE_1496-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42776; Mature: 42776

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPYKGKYHFTSESVTEGHPDKVADQISDAVLDVLLEQDPDSRVACETMVTTGMAIIAGE
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHEEEEC
ITTRGYADLPQVVRDTIKEIGYNNSSMGFDWQTCAVLSSIDKQSPDIAQGVDRQDPENQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
AGDQGMMFGFACDETPTLMPAPIFWAHQLSQKLTAVRKDGTVDFLRPDGKTQVSFEYVDG
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECC
RPVRINNVVVSTQHAEQASQTDIIDAIRTHVIRPVLEPSGFFNEKDCEIFINTTGRFVIG
CEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEC
GPMGDCGLTGRKIIQDTYGGMGSHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPK
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCC
CEVQIAYCIGVADPVSVLCTSLGSSDISDEVLTQAVRDVFDLRPYHISKRLDLRRPIYKK
CEEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
SACYGHFGREIPEFSWERTDAVDDLRAAARR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIPYKGKYHFTSESVTEGHPDKVADQISDAVLDVLLEQDPDSRVACETMVTTGMAIIAGE
CCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCHHEEEEC
ITTRGYADLPQVVRDTIKEIGYNNSSMGFDWQTCAVLSSIDKQSPDIAQGVDRQDPENQG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCC
AGDQGMMFGFACDETPTLMPAPIFWAHQLSQKLTAVRKDGTVDFLRPDGKTQVSFEYVDG
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEECC
RPVRINNVVVSTQHAEQASQTDIIDAIRTHVIRPVLEPSGFFNEKDCEIFINTTGRFVIG
CEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEC
GPMGDCGLTGRKIIQDTYGGMGSHGGGAFSGKDPSKVDRSGAYMGRYIAKNVVAAGLAPK
CCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHHCCCCC
CEVQIAYCIGVADPVSVLCTSLGSSDISDEVLTQAVRDVFDLRPYHISKRLDLRRPIYKK
CEEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHH
SACYGHFGREIPEFSWERTDAVDDLRAAARR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA