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The map label for this gene is merA [H]

Identifier: 78356508

GI number: 78356508

Start: 1484275

End: 1485735

Strand: Direct

Name: merA [H]

Synonym: Dde_1463

Alternate gene names: 78356508

Gene position: 1484275-1485735 (Clockwise)

Preceding gene: 78356507

Following gene: 78356517

Centisome position: 39.79

GC content: 59.82

Gene sequence:

>1461_bases
ATGATGGCAAAATATGACTATGATATCATCGTGATAGGCGGCGGTGCCGCCGGTCTGACCGTAACAGCCGGAGCCGCTCA
GCTGGGCGTTAAGGTGTTACTGGTGGAAAGCGGGCATGCCCTGGGCGGCGATTGTCTTCATTACGGCTGCGTACCCAGCA
AAACACTGCTGCGTACCGCCGGAGTGCGGCATCTGATGCGGCACGCGGCACGTTACGGACTGCCCGATGCGCAGCTGCCG
CCGGTGGACTTTGCGCAGGTGGCGCAGCGTATCAGCGAAGTTCAGGCCGTCATCCAGCAGCATGATTCCGTGGAACGTTT
CACTGCACTGGGAGCAGAGGTGCTTTTCGGTGCGGCGTCGTTTGCGGATGACCACACGGTGGAAATCCGTTCTGATGATT
CTGTGGTGCGGGCCACAGGAGCAAAAATTGTCATCGCCACCGGGTCGGGTGCCTCGGTGCCCCCGCTGGAGGGGCTGAAA
GAAAGCGGATATCTGACCAACAGAGAGATTTTTTCTCTGCCGGTGCTGCCGGCATCGCTGATAGTGCTGGGGGGCGGGCC
TGTGGCTCTTGAGATGGCGCAGGCCTTCCGGCGTCTGGGAACAGAAGTGACCGTTGTACAGCGTTCGGGGCAGTTGCTGA
GCAACGAGGATGCCGATATGGCGGATATCGTCAGGCTGCGGCTGGAATCTGAAGGGGTGCGTGTGCTTACCCGCACGGAG
ATACAGTCTATCCGGCGCGGAGCTGACGGTGTGCAGGTGCGCCTTGTACACGAGGGATCGGAAAAACTGTTGTCTGCTGC
AGAGTTGCTGGTGGCTCTGGGGCGTGCTCCTCATGTGTCGGGACTGACGCTGGAAAATGCAGGTGTGGTGTATTCGGCGC
GGGGGGTCGGGGTGGATGCACGCATGCGGACCAACGTACCGCACATCTATGCCGCCGGAGACGTGACCGGCCGCTACCTT
TTCACCCATGCGGCAGGGTACGAGGGGGGCATCATCATTGCCAATGCCGTATTCCGTCTGCCGAAAAAGGCTGATTATAC
CAACATGCCGTGGTGCACGTTTACTGATCCGGAGCTGGCATCCGTGGGGCTGAACGAACGGCGGGCGCAGGCCGCCGGAG
TCGATTATACCGTCCGCACTGAGTTGTTTTCAGGAAACGACAGGGCACTGGCCGAAGGAGCACCGGAAGGGCGCATAAAA
ATGCTGCTTGACCCGCGGGAAAAAGTGCTGGGGGTGCAGATATGCGGTGCCGGCGCCGGTGAAATCATCAACCAGTGGGT
GGCTGTGCAGGCCGGGAAGGTTTCATTGTCCAGAATTGCCGGTGCGGTGTATCCGTATCCGACGCTGGGCGAAATAAGCA
AGCGGGTGGCCGGCAATGTGATGGCGGACAAATTGTTCTCCGGCAAGGTGCAGAGTGCACTGTGTTTTCTTTTTCGCCAT
CAGGGCAAGGCGTGCAGCTAG

Upstream 100 bases:

>100_bases
CTTCGCTGATACTGTCTTTTGCCCTTCTGGGGCTTTTTCCGCTGGCAGCGCGCAAGGTGCTGGAACGTGCAGCCGCATGG
CGCAGCACAAAAGGAGCCTG

Downstream 100 bases:

>100_bases
CTTGCTCCGCTACTGCAGTCTTTCCGGCTGCCGGATGCGCTGCATGAGCACGCTCCATGTGGCTACCAGCGTGTCGTTCT
GAAACAGTGCCCCTCTGTTT

Product: mercuric reductase

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MMAKYDYDIIVIGGGAAGLTVTAGAAQLGVKVLLVESGHALGGDCLHYGCVPSKTLLRTAGVRHLMRHAARYGLPDAQLP
PVDFAQVAQRISEVQAVIQQHDSVERFTALGAEVLFGAASFADDHTVEIRSDDSVVRATGAKIVIATGSGASVPPLEGLK
ESGYLTNREIFSLPVLPASLIVLGGGPVALEMAQAFRRLGTEVTVVQRSGQLLSNEDADMADIVRLRLESEGVRVLTRTE
IQSIRRGADGVQVRLVHEGSEKLLSAAELLVALGRAPHVSGLTLENAGVVYSARGVGVDARMRTNVPHIYAAGDVTGRYL
FTHAAGYEGGIIIANAVFRLPKKADYTNMPWCTFTDPELASVGLNERRAQAAGVDYTVRTELFSGNDRALAEGAPEGRIK
MLLDPREKVLGVQICGAGAGEIINQWVAVQAGKVSLSRIAGAVYPYPTLGEISKRVAGNVMADKLFSGKVQSALCFLFRH
QGKACS

Sequences:

>Translated_486_residues
MMAKYDYDIIVIGGGAAGLTVTAGAAQLGVKVLLVESGHALGGDCLHYGCVPSKTLLRTAGVRHLMRHAARYGLPDAQLP
PVDFAQVAQRISEVQAVIQQHDSVERFTALGAEVLFGAASFADDHTVEIRSDDSVVRATGAKIVIATGSGASVPPLEGLK
ESGYLTNREIFSLPVLPASLIVLGGGPVALEMAQAFRRLGTEVTVVQRSGQLLSNEDADMADIVRLRLESEGVRVLTRTE
IQSIRRGADGVQVRLVHEGSEKLLSAAELLVALGRAPHVSGLTLENAGVVYSARGVGVDARMRTNVPHIYAAGDVTGRYL
FTHAAGYEGGIIIANAVFRLPKKADYTNMPWCTFTDPELASVGLNERRAQAAGVDYTVRTELFSGNDRALAEGAPEGRIK
MLLDPREKVLGVQICGAGAGEIINQWVAVQAGKVSLSRIAGAVYPYPTLGEISKRVAGNVMADKLFSGKVQSALCFLFRH
QGKACS
>Mature_486_residues
MMAKYDYDIIVIGGGAAGLTVTAGAAQLGVKVLLVESGHALGGDCLHYGCVPSKTLLRTAGVRHLMRHAARYGLPDAQLP
PVDFAQVAQRISEVQAVIQQHDSVERFTALGAEVLFGAASFADDHTVEIRSDDSVVRATGAKIVIATGSGASVPPLEGLK
ESGYLTNREIFSLPVLPASLIVLGGGPVALEMAQAFRRLGTEVTVVQRSGQLLSNEDADMADIVRLRLESEGVRVLTRTE
IQSIRRGADGVQVRLVHEGSEKLLSAAELLVALGRAPHVSGLTLENAGVVYSARGVGVDARMRTNVPHIYAAGDVTGRYL
FTHAAGYEGGIIIANAVFRLPKKADYTNMPWCTFTDPELASVGLNERRAQAAGVDYTVRTELFSGNDRALAEGAPEGRIK
MLLDPREKVLGVQICGAGAGEIINQWVAVQAGKVSLSRIAGAVYPYPTLGEISKRVAGNVMADKLFSGKVQSALCFLFRH
QGKACS

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=467, Percent_Identity=29.5503211991435, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=30.0429184549356, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI22035672, Length=483, Percent_Identity=32.2981366459627, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI291045266, Length=477, Percent_Identity=27.6729559748428, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI148277065, Length=476, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519430, Length=476, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519428, Length=476, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI33519426, Length=476, Percent_Identity=26.0504201680672, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI148277071, Length=476, Percent_Identity=26.0504201680672, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045268, Length=471, Percent_Identity=26.3269639065817, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=29.004329004329, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081717, Length=451, Percent_Identity=29.2682926829268, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=30.7368421052632, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=27.3563218390805, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=455, Percent_Identity=31.8681318681319, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.5135699373695, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=372, Percent_Identity=28.494623655914, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=372, Percent_Identity=28.494623655914, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=25.9259259259259, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=31.5899581589958, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=29.8507462686567, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=487, Percent_Identity=28.3367556468172, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=30.4721030042918, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=29.769392033543, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=29.769392033543, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=29.769392033543, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17737741, Length=481, Percent_Identity=27.4428274428274, Blast_Score=142, Evalue=5e-34,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 51507; Mature: 51507

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKYDYDIIVIGGGAAGLTVTAGAAQLGVKVLLVESGHALGGDCLHYGCVPSKTLLRTA
CCCEECCEEEEECCCCCCEEEECCHHHCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHH
GVRHLMRHAARYGLPDAQLPPVDFAQVAQRISEVQAVIQQHDSVERFTALGAEVLFGAAS
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
FADDHTVEIRSDDSVVRATGAKIVIATGSGASVPPLEGLKESGYLTNREIFSLPVLPASL
CCCCCEEEEECCCCEEEECCCEEEEEECCCCCCCCHHCCCCCCCEECCCEEEECCCCEEE
IVLGGGPVALEMAQAFRRLGTEVTVVQRSGQLLSNEDADMADIVRLRLESEGVRVLTRTE
EEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEHHH
IQSIRRGADGVQVRLVHEGSEKLLSAAELLVALGRAPHVSGLTLENAGVVYSARGVGVDA
HHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCC
RMRTNVPHIYAAGDVTGRYLFTHAAGYEGGIIIANAVFRLPKKADYTNMPWCTFTDPELA
EEECCCCEEEEECCCCCEEEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCEEECCCCHHH
SVGLNERRAQAAGVDYTVRTELFSGNDRALAEGAPEGRIKMLLDPREKVLGVQICGAGAG
HCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHEEEEEEECCCHH
EIINQWVAVQAGKVSLSRIAGAVYPYPTLGEISKRVAGNVMADKLFSGKVQSALCFLFRH
HHHHHHHHEECCCEEHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHC
QGKACS
CCCCCC
>Mature Secondary Structure
MMAKYDYDIIVIGGGAAGLTVTAGAAQLGVKVLLVESGHALGGDCLHYGCVPSKTLLRTA
CCCEECCEEEEECCCCCCEEEECCHHHCCEEEEEEECCCCCCCCCEEECCCCCHHHHHHH
GVRHLMRHAARYGLPDAQLPPVDFAQVAQRISEVQAVIQQHDSVERFTALGAEVLFGAAS
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
FADDHTVEIRSDDSVVRATGAKIVIATGSGASVPPLEGLKESGYLTNREIFSLPVLPASL
CCCCCEEEEECCCCEEEECCCEEEEEECCCCCCCCHHCCCCCCCEECCCEEEECCCCEEE
IVLGGGPVALEMAQAFRRLGTEVTVVQRSGQLLSNEDADMADIVRLRLESEGVRVLTRTE
EEECCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCEEEEHHH
IQSIRRGADGVQVRLVHEGSEKLLSAAELLVALGRAPHVSGLTLENAGVVYSARGVGVDA
HHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCCCC
RMRTNVPHIYAAGDVTGRYLFTHAAGYEGGIIIANAVFRLPKKADYTNMPWCTFTDPELA
EEECCCCEEEEECCCCCEEEEEECCCCCCCEEEEEHHHHCCCCCCCCCCCEEECCCCHHH
SVGLNERRAQAAGVDYTVRTELFSGNDRALAEGAPEGRIKMLLDPREKVLGVQICGAGAG
HCCCCHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCHHEEEEEEECCCHH
EIINQWVAVQAGKVSLSRIAGAVYPYPTLGEISKRVAGNVMADKLFSGKVQSALCFLFRH
HHHHHHHHEECCCEEHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHC
QGKACS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]