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The map label for this gene is trmFO

Identifier: 78356467

GI number: 78356467

Start: 1446597

End: 1447973

Strand: Reverse

Name: trmFO

Synonym: Dde_1422

Alternate gene names: 78356467

Gene position: 1447973-1446597 (Counterclockwise)

Preceding gene: 78356468

Following gene: 78356461

Centisome position: 38.82

GC content: 59.69

Gene sequence:

>1377_bases
ATGGCACAAAAACACACCAAACAATCGGAGAACGCCTTGACCGCTCTTACCTCTGCGGCAATTGTCGGCGGCGGCCTTGC
CGGATGCGAATGCGCCCGCAAGCTGGCACGCGCCGGTATCGCCGTGACTCTTTTTGAAATGAAGCCCCACAGCTTTTCAC
CCGCCCATTCCTCGCCCGAACTGGGCGAGCTGGTATGTTCCAACTCGTTGCGCTCCGACGAGCCCGAAGCGGGCGTCGGC
GTTTTGAAACAGGAAATGCGCGCACTGGGCAGTCTGGTGATGGAAGTGGCAGAAGCCACGCGTGTTCCGGCCGGTAAAGC
ACTGGCAGTCGACAGAGGTCTTTTCGCAGCCTCCATGACGGCAGCCATGGAGGCTGAACCGGGCATCACGCTGATACGTC
GGGAAATCACTTCGCTGGACGACCCCGCCCTGCAGGGGTTCGACGCGGTGGTCATCGCCGCCGGTCCTGTGGCCAGCGAA
AGCCTGAGCCGCAGCCTTGCCGATGCAGTGGGGGCGACACACCTGTATTTCTACGACGCCATAGCCCCCATCATCAGCGC
AGACTCCGTAAATATGGAAAAAGCCTTCTGGGGTTCACGCTATCGCCCGGAAGACACCGACTATCTGAACTGCCCCATGA
ACCGCGAAGAATATTTCGCCTTTCGCGAGGCGCTGGTGGAAGGCGAAAAGGCCGCTACCAAAGATTTTGAAAAAGAACTG
CACTTTGAAGGCTGCATGCCCATCGAGGCGCTGGCCGAGCGGGGAGAAATGACGTTGGCGTTCGGCCCGTTCAAACCCGT
GGGACTGGACGATCCGCGCACCGGCAGACGCCCCTTTGCCGTGGTGCAGCTGCGTACCGAAAACCTGAACAAAACCATGT
TCAACCTTGTGGGCTGTCAGACAAAGCTCAAGTACGGTGAACAGGACAGGATTTTCCGCATGATTCCGGGTCTGGAAGAC
GCGGAATTCGTGCGCTACGGCTCGGTGCACCGCAACACCTACGTCAATGCGCCGCAGGTGCTCAACGGCGACCAGTCGCT
GAAAAACCGCCCCGACGTATTTCTGGCCGGACAAATCACCGGAGTGGAAGGCTATGTGGAGTCCGCCGCCAACGGCATGT
GGCTGGGCATGATGCTTGCGGCCCGCAAGCACGGAGAAAACATCCCCCAGCCGCCTGTTGAGTGCGCTCTGGGTGCGCTG
GCGGCCCACCTGCGCACTCCGGTAAAAAAGTTTCAGCCTTCCAACATCAACTTCGGTCTGACTCCGGAGCTGAATCAGCG
GGCACGCAAAAAAGACCGCAAGGCCCTGTACGCCGAACGGGCACGCGAAAACTTTACACAGTGGTACGCGTTGCTTCCCG
CATCAGCCCGCAGCTGA

Upstream 100 bases:

>100_bases
CTGAAAGCGCTGCACAGCAGGCTGTACGACGCATATATGCCGGGCATGTGACGGCACGGGCCGCTGCCTCTTGCCTTGCG
CCCCGCAATGGCATAGGTGC

Downstream 100 bases:

>100_bases
AAGTACCGTTAGCTCCGCAGCAAAAAACCGGCGGAACGTCATGCGTTCCGCCGGTTTTCTTATCCACTGACGCCCCTGCG
CAGCAGAGTTGCACCGTCCC

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MAQKHTKQSENALTALTSAAIVGGGLAGCECARKLARAGIAVTLFEMKPHSFSPAHSSPELGELVCSNSLRSDEPEAGVG
VLKQEMRALGSLVMEVAEATRVPAGKALAVDRGLFAASMTAAMEAEPGITLIRREITSLDDPALQGFDAVVIAAGPVASE
SLSRSLADAVGATHLYFYDAIAPIISADSVNMEKAFWGSRYRPEDTDYLNCPMNREEYFAFREALVEGEKAATKDFEKEL
HFEGCMPIEALAERGEMTLAFGPFKPVGLDDPRTGRRPFAVVQLRTENLNKTMFNLVGCQTKLKYGEQDRIFRMIPGLED
AEFVRYGSVHRNTYVNAPQVLNGDQSLKNRPDVFLAGQITGVEGYVESAANGMWLGMMLAARKHGENIPQPPVECALGAL
AAHLRTPVKKFQPSNINFGLTPELNQRARKKDRKALYAERARENFTQWYALLPASARS

Sequences:

>Translated_458_residues
MAQKHTKQSENALTALTSAAIVGGGLAGCECARKLARAGIAVTLFEMKPHSFSPAHSSPELGELVCSNSLRSDEPEAGVG
VLKQEMRALGSLVMEVAEATRVPAGKALAVDRGLFAASMTAAMEAEPGITLIRREITSLDDPALQGFDAVVIAAGPVASE
SLSRSLADAVGATHLYFYDAIAPIISADSVNMEKAFWGSRYRPEDTDYLNCPMNREEYFAFREALVEGEKAATKDFEKEL
HFEGCMPIEALAERGEMTLAFGPFKPVGLDDPRTGRRPFAVVQLRTENLNKTMFNLVGCQTKLKYGEQDRIFRMIPGLED
AEFVRYGSVHRNTYVNAPQVLNGDQSLKNRPDVFLAGQITGVEGYVESAANGMWLGMMLAARKHGENIPQPPVECALGAL
AAHLRTPVKKFQPSNINFGLTPELNQRARKKDRKALYAERARENFTQWYALLPASARS
>Mature_457_residues
AQKHTKQSENALTALTSAAIVGGGLAGCECARKLARAGIAVTLFEMKPHSFSPAHSSPELGELVCSNSLRSDEPEAGVGV
LKQEMRALGSLVMEVAEATRVPAGKALAVDRGLFAASMTAAMEAEPGITLIRREITSLDDPALQGFDAVVIAAGPVASES
LSRSLADAVGATHLYFYDAIAPIISADSVNMEKAFWGSRYRPEDTDYLNCPMNREEYFAFREALVEGEKAATKDFEKELH
FEGCMPIEALAERGEMTLAFGPFKPVGLDDPRTGRRPFAVVQLRTENLNKTMFNLVGCQTKLKYGEQDRIFRMIPGLEDA
EFVRYGSVHRNTYVNAPQVLNGDQSLKNRPDVFLAGQITGVEGYVESAANGMWLGMMLAARKHGENIPQPPVECALGALA
AHLRTPVKKFQPSNINFGLTPELNQRARKKDRKALYAERARENFTQWYALLPASARS

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_DESDG (Q312C5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387916.1
- ProteinModelPortal:   Q312C5
- STRING:   Q312C5
- GeneID:   3756131
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1422
- NMPDR:   fig|207559.3.peg.1794
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   DDES207559:DDE_1422-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 49959; Mature: 49827

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQKHTKQSENALTALTSAAIVGGGLAGCECARKLARAGIAVTLFEMKPHSFSPAHSSPE
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCH
LGELVCSNSLRSDEPEAGVGVLKQEMRALGSLVMEVAEATRVPAGKALAVDRGLFAASMT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
AAMEAEPGITLIRREITSLDDPALQGFDAVVIAAGPVASESLSRSLADAVGATHLYFYDA
HHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
IAPIISADSVNMEKAFWGSRYRPEDTDYLNCPMNREEYFAFREALVEGEKAATKDFEKEL
HHHHHCCCCCCHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
HFEGCMPIEALAERGEMTLAFGPFKPVGLDDPRTGRRPFAVVQLRTENLNKTMFNLVGCQ
CCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
TKLKYGEQDRIFRMIPGLEDAEFVRYGSVHRNTYVNAPQVLNGDQSLKNRPDVFLAGQIT
HHHCCCCCCCHHHHCCCCCCCHHHHHCCHHCCCCCCCCHHCCCCHHHCCCCCEEEEEEEE
GVEGYVESAANGMWLGMMLAARKHGENIPQPPVECALGALAAHLRTPVKKFQPSNINFGL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPELNQRARKKDRKALYAERARENFTQWYALLPASARS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQKHTKQSENALTALTSAAIVGGGLAGCECARKLARAGIAVTLFEMKPHSFSPAHSSPE
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCH
LGELVCSNSLRSDEPEAGVGVLKQEMRALGSLVMEVAEATRVPAGKALAVDRGLFAASMT
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
AAMEAEPGITLIRREITSLDDPALQGFDAVVIAAGPVASESLSRSLADAVGATHLYFYDA
HHHCCCCCHHHHHHHHHCCCCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
IAPIISADSVNMEKAFWGSRYRPEDTDYLNCPMNREEYFAFREALVEGEKAATKDFEKEL
HHHHHCCCCCCHHHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
HFEGCMPIEALAERGEMTLAFGPFKPVGLDDPRTGRRPFAVVQLRTENLNKTMFNLVGCQ
CCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHH
TKLKYGEQDRIFRMIPGLEDAEFVRYGSVHRNTYVNAPQVLNGDQSLKNRPDVFLAGQIT
HHHCCCCCCCHHHHCCCCCCCHHHHHCCHHCCCCCCCCHHCCCCHHHCCCCCEEEEEEEE
GVEGYVESAANGMWLGMMLAARKHGENIPQPPVECALGALAAHLRTPVKKFQPSNINFGL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPELNQRARKKDRKALYAERARENFTQWYALLPASARS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA