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The map label for this gene is lptD [H]

Identifier: 78356458

GI number: 78356458

Start: 1430377

End: 1432710

Strand: Direct

Name: lptD [H]

Synonym: Dde_1413

Alternate gene names: 78356458

Gene position: 1430377-1432710 (Clockwise)

Preceding gene: 78356453

Following gene: 78356459

Centisome position: 38.35

GC content: 55.53

Gene sequence:

>2334_bases
ATGCGAAAAGACTGTCGAAATTTATTTTTCTGCTGCGTTGTTTTTCTGACGTGCTTTCTGCTGACCGGGCATGGCGCGGC
GCTGGCCGGCACCATTTCGGTGACGGATAGCGAGCAGGAAACCGTTCACTGGAATCTGACCGCGGACAGGGTTGTCACCC
ATAGTGACAGCAATATGATCGAAGCGTTCGGCAATGTGGCGCTCCGCGAAGGCGACGATTTTCTGAAAGCTGATTTTATC
AGATATTTCGGCGATACGGACTGGGTGCTGCTGAAGGGCAATGTAAGCGTAAAAATGGGGCGCGACGTGGTGGACGCCTC
TGAAGCCGAATTTGACCTGAAAAACAGAGTAGGATGGCTGCGCGACGGCTCGGTGTTTGTTGACGGACCGCATATTTATT
TTTCCGGTGAGAGAGTGCAGAAGGACTTCGGCGATTCATACCGGTTCCGCAATGCCAAGATAACCGCGTGTGACGGCGAA
GTGCCCGCATGGTCCATAACCGCGCGTGAAGCCACCATCGAGCTTGAAGGGTATTCACAGCTCTGGCATACCAGCATGCA
GGTGAAGGACCACAGCGTGCTGTACGCTCCGTACGCCATTCTGCCTGCCAAAAAGAAACGGCAGACAGGCTTTCTGCTGC
CCGAGGTGGGGCAGAGCTCAAAGCGCGGTCTGTATTATAACCAGCCATTTTTCTGGGCCATCAGCGACACGGCAGACCTG
ACGTACAATGCATACTGGATGGAGTTGCGCGGGCTGATGCAGGGGCTTGAGTTTCGTCACCACACCACTGAAGACAATAA
AAGCTGGCTGCGTGTCGACTACCTGAAGGATGCCCGTACAGACAGCACCGAAGAAGACGAGCCGGGCGGACTGGACGATG
ATGGTCTGGTGCGGAACAACGATGACAGATTCTGGGTGCGCGGTATGTTCGACGGCACGCTGCACGATACGCAGTGGAAG
CTGAAAGCCACGGTGGACTATGTTTCCGACCAGAATTACCTGCGTGAATTTTCGTCCGGTATGAGCGGTTTCAACCGCAG
TCAGGACGATATGGAAGATCTGTTCGGCCGCAGCCTGGCCGGAGAGGCGCAGAACAGAACCAGTGAAGTGCTTCTTTCCC
GTGACTGGCAGCGGGTGGGGCTTGGCATCAGTTCCAGCTTTACCCAGAACGTCAACGTGGGGAACGGCAACCTTACCTCT
TCTGCCGATAATACCGTGCAGACCCTGCCCAAGATCGACCTGTATCTTTTTAAAAGTAACCTTGTGGACGGCGCCGCCCT
GCCGGTGGAGATTCAGGCCACGGCACAGGCTGCCAACTTCTGGCGGCACAACGGTACCACCGGCGCACGCTTTGAAGTGG
CTCCGCAGATCAGCATTCCCCTTTCCGGCCGGTACGGCTCCGTCATTTCCACTGTGGGATTGCGCCATACGCAGTATTCC
ACCAGTCACAAAGAACCGGACGGTATACAGGAACAAAGTGCCACCGGTACGTCGCGTACCATTCCCAGCCTGAATATTGC
GGCTTTTACCGAAATGGCGCGCACTTTCAGTCTGGCAGGGCAGGACCCTGCCGCAGGAATGAAAGCCGGAGATTCTCGCT
GGACAATGCTGCGGCACAGTATTCAGCCACGTGTGAGTTACGACAATACCCCCAATGTCAATCAGGACGACAACCCGTTC
TACGATGTGGACGACCGTATTCTGGCGCGTAACGAGCTGACCTATTCGGTGACCAACCTGTTCACGCGCAAGCGGCTGAG
TGTGAGCGCGGGGGCTGATGAGGAAAATCCCGAACTGGTGCAGCGCTACGATTATTCCGATTTTCTGCGGTTGCGGCTGC
AGCAGAGCTACGACTTCCGCGAAGCCGACCGCACCGAGCAGCTTGAAAAGTACAGCCGGCGTCCTTTTTCCGATGTGGAG
CTGGAAGCGCGCCTCAGCTACGACAACATCGCATCGCTCACCAGCCGCACCTATTGGTCTCCGTATATCGGCATGGTGAC
CCGCCATGAGCATACGCTGGATTTCACGTATTACGAGCGGGCTACGTTCCGCACCACGTTTGACTTCCGCCAGCAGGTGG
ACGAGTACAAGCGTAAAGAAACAGAAACGGATGACAAGCTGAGCTCCATGAATTCAGAACTGGAGCTGAAGCTGTGGGGG
CCGTGGACGCTGGGACTTAACTACGAAGTGGATCTGGAAAAAGGCAAAGATATAGAGAAAGGCGTGGATCTGCTGTATTC
GCATCAGTGTTTCGATCTGCAGTTCAGCTACGCCAAAGACGGCGTGGAAGACGAGTTCTCGTTGTGGCTGGCTTTGCCCG
GCCTTAGCTTCTGA

Upstream 100 bases:

>100_bases
GCCGCGTGGCCGATGTACCCTTTTTTCGGGGCATCTTTTTGTAATATCAAAGAAATCAATCTGCCCGGATTGCTGCGGGA
CTACCACGAGAACGCTGTTG

Downstream 100 bases:

>100_bases
TTATCCTGCCTGTCTGCAAACGTGTACTCGCAGCGCTGCGGAGCTTTCCTCCGCAGCGCTTTTTTTTGCGGAACAGGCGC
GTGGGGCGCAATCAATATAC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 777; Mature: 777

Protein sequence:

>777_residues
MRKDCRNLFFCCVVFLTCFLLTGHGAALAGTISVTDSEQETVHWNLTADRVVTHSDSNMIEAFGNVALREGDDFLKADFI
RYFGDTDWVLLKGNVSVKMGRDVVDASEAEFDLKNRVGWLRDGSVFVDGPHIYFSGERVQKDFGDSYRFRNAKITACDGE
VPAWSITAREATIELEGYSQLWHTSMQVKDHSVLYAPYAILPAKKKRQTGFLLPEVGQSSKRGLYYNQPFFWAISDTADL
TYNAYWMELRGLMQGLEFRHHTTEDNKSWLRVDYLKDARTDSTEEDEPGGLDDDGLVRNNDDRFWVRGMFDGTLHDTQWK
LKATVDYVSDQNYLREFSSGMSGFNRSQDDMEDLFGRSLAGEAQNRTSEVLLSRDWQRVGLGISSSFTQNVNVGNGNLTS
SADNTVQTLPKIDLYLFKSNLVDGAALPVEIQATAQAANFWRHNGTTGARFEVAPQISIPLSGRYGSVISTVGLRHTQYS
TSHKEPDGIQEQSATGTSRTIPSLNIAAFTEMARTFSLAGQDPAAGMKAGDSRWTMLRHSIQPRVSYDNTPNVNQDDNPF
YDVDDRILARNELTYSVTNLFTRKRLSVSAGADEENPELVQRYDYSDFLRLRLQQSYDFREADRTEQLEKYSRRPFSDVE
LEARLSYDNIASLTSRTYWSPYIGMVTRHEHTLDFTYYERATFRTTFDFRQQVDEYKRKETETDDKLSSMNSELELKLWG
PWTLGLNYEVDLEKGKDIEKGVDLLYSHQCFDLQFSYAKDGVEDEFSLWLALPGLSF

Sequences:

>Translated_777_residues
MRKDCRNLFFCCVVFLTCFLLTGHGAALAGTISVTDSEQETVHWNLTADRVVTHSDSNMIEAFGNVALREGDDFLKADFI
RYFGDTDWVLLKGNVSVKMGRDVVDASEAEFDLKNRVGWLRDGSVFVDGPHIYFSGERVQKDFGDSYRFRNAKITACDGE
VPAWSITAREATIELEGYSQLWHTSMQVKDHSVLYAPYAILPAKKKRQTGFLLPEVGQSSKRGLYYNQPFFWAISDTADL
TYNAYWMELRGLMQGLEFRHHTTEDNKSWLRVDYLKDARTDSTEEDEPGGLDDDGLVRNNDDRFWVRGMFDGTLHDTQWK
LKATVDYVSDQNYLREFSSGMSGFNRSQDDMEDLFGRSLAGEAQNRTSEVLLSRDWQRVGLGISSSFTQNVNVGNGNLTS
SADNTVQTLPKIDLYLFKSNLVDGAALPVEIQATAQAANFWRHNGTTGARFEVAPQISIPLSGRYGSVISTVGLRHTQYS
TSHKEPDGIQEQSATGTSRTIPSLNIAAFTEMARTFSLAGQDPAAGMKAGDSRWTMLRHSIQPRVSYDNTPNVNQDDNPF
YDVDDRILARNELTYSVTNLFTRKRLSVSAGADEENPELVQRYDYSDFLRLRLQQSYDFREADRTEQLEKYSRRPFSDVE
LEARLSYDNIASLTSRTYWSPYIGMVTRHEHTLDFTYYERATFRTTFDFRQQVDEYKRKETETDDKLSSMNSELELKLWG
PWTLGLNYEVDLEKGKDIEKGVDLLYSHQCFDLQFSYAKDGVEDEFSLWLALPGLSF
>Mature_777_residues
MRKDCRNLFFCCVVFLTCFLLTGHGAALAGTISVTDSEQETVHWNLTADRVVTHSDSNMIEAFGNVALREGDDFLKADFI
RYFGDTDWVLLKGNVSVKMGRDVVDASEAEFDLKNRVGWLRDGSVFVDGPHIYFSGERVQKDFGDSYRFRNAKITACDGE
VPAWSITAREATIELEGYSQLWHTSMQVKDHSVLYAPYAILPAKKKRQTGFLLPEVGQSSKRGLYYNQPFFWAISDTADL
TYNAYWMELRGLMQGLEFRHHTTEDNKSWLRVDYLKDARTDSTEEDEPGGLDDDGLVRNNDDRFWVRGMFDGTLHDTQWK
LKATVDYVSDQNYLREFSSGMSGFNRSQDDMEDLFGRSLAGEAQNRTSEVLLSRDWQRVGLGISSSFTQNVNVGNGNLTS
SADNTVQTLPKIDLYLFKSNLVDGAALPVEIQATAQAANFWRHNGTTGARFEVAPQISIPLSGRYGSVISTVGLRHTQYS
TSHKEPDGIQEQSATGTSRTIPSLNIAAFTEMARTFSLAGQDPAAGMKAGDSRWTMLRHSIQPRVSYDNTPNVNQDDNPF
YDVDDRILARNELTYSVTNLFTRKRLSVSAGADEENPELVQRYDYSDFLRLRLQQSYDFREADRTEQLEKYSRRPFSDVE
LEARLSYDNIASLTSRTYWSPYIGMVTRHEHTLDFTYYERATFRTTFDFRQQVDEYKRKETETDDKLSSMNSELELKLWG
PWTLGLNYEVDLEKGKDIEKGVDLLYSHQCFDLQFSYAKDGVEDEFSLWLALPGLSF

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=474, Percent_Identity=21.9409282700422, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 88599; Mature: 88599

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKDCRNLFFCCVVFLTCFLLTGHGAALAGTISVTDSEQETVHWNLTADRVVTHSDSNMI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEECCEEEEECCCHHH
EAFGNVALREGDDFLKADFIRYFGDTDWVLLKGNVSVKMGRDVVDASEAEFDLKNRVGWL
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCHHCCCCCCEEHHHCCCEE
RDGSVFVDGPHIYFSGERVQKDFGDSYRFRNAKITACDGEVPAWSITAREATIELEGYSQ
ECCCEEEECCEEEECCHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEEEEEEEECCCHH
LWHTSMQVKDHSVLYAPYAILPAKKKRQTGFLLPEVGQSSKRGLYYNQPFFWAISDTADL
HHHHHEEECCCEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCC
TYNAYWMELRGLMQGLEFRHHTTEDNKSWLRVDYLKDARTDSTEEDEPGGLDDDGLVRNN
EEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEECC
DDRFWVRGMFDGTLHDTQWKLKATVDYVSDQNYLREFSSGMSGFNRSQDDMEDLFGRSLA
CCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
GEAQNRTSEVLLSRDWQRVGLGISSSFTQNVNVGNGNLTSSADNTVQTLPKIDLYLFKSN
CCHHCHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCCEEEEEEECC
LVDGAALPVEIQATAQAANFWRHNGTTGARFEVAPQISIPLSGRYGSVISTVGLRHTQYS
CCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCHHHHHHCCCCCCCC
TSHKEPDGIQEQSATGTSRTIPSLNIAAFTEMARTFSLAGQDPAAGMKAGDSRWTMLRHS
CCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
IQPRVSYDNTPNVNQDDNPFYDVDDRILARNELTYSVTNLFTRKRLSVSAGADEENPELV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEHHHHHHHHHHEEEECCCCCCCHHHH
QRYDYSDFLRLRLQQSYDFREADRTEQLEKYSRRPFSDVELEARLSYDNIASLTSRTYWS
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHCCCCC
PYIGMVTRHEHTLDFTYYERATFRTTFDFRQQVDEYKRKETETDDKLSSMNSELELKLWG
CHHCEEECCCCEEEEEEEECEEEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEC
PWTLGLNYEVDLEKGKDIEKGVDLLYSHQCFDLQFSYAKDGVEDEFSLWLALPGLSF
CEEECCEEEEECCCCCCHHHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MRKDCRNLFFCCVVFLTCFLLTGHGAALAGTISVTDSEQETVHWNLTADRVVTHSDSNMI
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEECCEEEEECCCHHH
EAFGNVALREGDDFLKADFIRYFGDTDWVLLKGNVSVKMGRDVVDASEAEFDLKNRVGWL
HHHCCEEEECCCCHHHHHHHHHCCCCCEEEEECCEEEEECCCHHCCCCCCEEHHHCCCEE
RDGSVFVDGPHIYFSGERVQKDFGDSYRFRNAKITACDGEVPAWSITAREATIELEGYSQ
ECCCEEEECCEEEECCHHHHHHCCCCEEECCCEEEEECCCCCEEEEEEEEEEEEECCCHH
LWHTSMQVKDHSVLYAPYAILPAKKKRQTGFLLPEVGQSSKRGLYYNQPFFWAISDTADL
HHHHHEEECCCEEEEECEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCC
TYNAYWMELRGLMQGLEFRHHTTEDNKSWLRVDYLKDARTDSTEEDEPGGLDDDGLVRNN
EEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEECC
DDRFWVRGMFDGTLHDTQWKLKATVDYVSDQNYLREFSSGMSGFNRSQDDMEDLFGRSLA
CCCEEEEEEECCCCCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCC
GEAQNRTSEVLLSRDWQRVGLGISSSFTQNVNVGNGNLTSSADNTVQTLPKIDLYLFKSN
CCHHCHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHCCCEEEEEEECC
LVDGAALPVEIQATAQAANFWRHNGTTGARFEVAPQISIPLSGRYGSVISTVGLRHTQYS
CCCCCCCCEEEEEHHHHHHHHHCCCCCCCEEEECCEEEEEECCCCCHHHHHHCCCCCCCC
TSHKEPDGIQEQSATGTSRTIPSLNIAAFTEMARTFSLAGQDPAAGMKAGDSRWTMLRHS
CCCCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
IQPRVSYDNTPNVNQDDNPFYDVDDRILARNELTYSVTNLFTRKRLSVSAGADEENPELV
CCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEHHHHHHHHHHEEEECCCCCCCHHHH
QRYDYSDFLRLRLQQSYDFREADRTEQLEKYSRRPFSDVELEARLSYDNIASLTSRTYWS
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHCCCCC
PYIGMVTRHEHTLDFTYYERATFRTTFDFRQQVDEYKRKETETDDKLSSMNSELELKLWG
CHHCEEECCCCEEEEEEEECEEEEEHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEEC
PWTLGLNYEVDLEKGKDIEKGVDLLYSHQCFDLQFSYAKDGVEDEFSLWLALPGLSF
CEEECCEEEEECCCCCCHHHHHHHHCCCCEEEEEEHHCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA