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The map label for this gene is nfsA [C]

Identifier: 78356400

GI number: 78356400

Start: 1370180

End: 1370689

Strand: Direct

Name: nfsA [C]

Synonym: Dde_1353

Alternate gene names: 78356400

Gene position: 1370180-1370689 (Clockwise)

Preceding gene: 78356398

Following gene: 78356401

Centisome position: 36.73

GC content: 60.78

Gene sequence:

>510_bases
ATGGAATTTAAAGATGTTGTACGTATGCGCCGCAGTGTGCGCAAGTTTACGGAGGAACCCGTGACTGAAGAGCAGCTGGC
GGCGCTGCTGGAAGCCGCCATGGCTGCGCCCAGCGCAGGCAATGCACAGCCGTGGCGTTTTATCGTGGTAGACGACAAAG
ATACGCTGGCCGCAGTGCCCGCCATTCACCCCTATGCGGCCATGGCAGCATCGGCGCCGCTGGCCGTGCTGGTGTGCGGT
GATCTTTCTGCAGAAAAATACCCCGGATTCTGGGTGCAGGACTGCGCCGCGGCAGTGCAGAATATGTTGCTGGCAGCGGT
GGACGCCGGTCTGGGGGCGGTCTGGACAGGAATTTATCCTGAGCAGGAGCGTGTGCGTCAGTTCAGTGCCACGTTCGGCC
TGCCCGAACATGTTGTGCCGCTGGCACTTGTTGTTGCGGGACACGCGGCAAAAACACCTGCTCCCGGTGGCCGCTTTGAT
GCAGCCAAAGTACGTCGCAACCGGTGGTAG

Upstream 100 bases:

>100_bases
TGTACCTGCACAAAAGACCTGTGTTGTGTTAGTATCTGTGCATATGTGTGCGATACAGCGGCAGCCGGTTGCTGTAGTCT
GCTTTTTTAAGGAGGATGAT

Downstream 100 bases:

>100_bases
ATTATCCGCACAGCCTGTGCTGCGGCTTGAATAACGGCGTAAAAACATATACCTGCCATGCAGAGGCGTGCAGTTGCCCG
CCCGCACCATAAAGGAGTGA

Product: nitroreductase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MEFKDVVRMRRSVRKFTEEPVTEEQLAALLEAAMAAPSAGNAQPWRFIVVDDKDTLAAVPAIHPYAAMAASAPLAVLVCG
DLSAEKYPGFWVQDCAAAVQNMLLAAVDAGLGAVWTGIYPEQERVRQFSATFGLPEHVVPLALVVAGHAAKTPAPGGRFD
AAKVRRNRW

Sequences:

>Translated_169_residues
MEFKDVVRMRRSVRKFTEEPVTEEQLAALLEAAMAAPSAGNAQPWRFIVVDDKDTLAAVPAIHPYAAMAASAPLAVLVCG
DLSAEKYPGFWVQDCAAAVQNMLLAAVDAGLGAVWTGIYPEQERVRQFSATFGLPEHVVPLALVVAGHAAKTPAPGGRFD
AAKVRRNRW
>Mature_169_residues
MEFKDVVRMRRSVRKFTEEPVTEEQLAALLEAAMAAPSAGNAQPWRFIVVDDKDTLAAVPAIHPYAAMAASAPLAVLVCG
DLSAEKYPGFWVQDCAAAVQNMLLAAVDAGLGAVWTGIYPEQERVRQFSATFGLPEHVVPLALVVAGHAAKTPAPGGRFD
AAKVRRNRW

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 18184; Mature: 18184

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKDVVRMRRSVRKFTEEPVTEEQLAALLEAAMAAPSAGNAQPWRFIVVDDKDTLAAVP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHCC
AIHPYAAMAASAPLAVLVCGDLSAEKYPGFWVQDCAAAVQNMLLAAVDAGLGAVWTGIYP
CCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
EQERVRQFSATFGLPEHVVPLALVVAGHAAKTPAPGGRFDAAKVRRNRW
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MEFKDVVRMRRSVRKFTEEPVTEEQLAALLEAAMAAPSAGNAQPWRFIVVDDKDTLAAVP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHCC
AIHPYAAMAASAPLAVLVCGDLSAEKYPGFWVQDCAAAVQNMLLAAVDAGLGAVWTGIYP
CCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
EQERVRQFSATFGLPEHVVPLALVVAGHAAKTPAPGGRFDAAKVRRNRW
CHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]