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The map label for this gene is opuE [H]

Identifier: 78356384

GI number: 78356384

Start: 1355665

End: 1357260

Strand: Reverse

Name: opuE [H]

Synonym: Dde_1337

Alternate gene names: 78356384

Gene position: 1357260-1355665 (Counterclockwise)

Preceding gene: 78356385

Following gene: 78356383

Centisome position: 36.39

GC content: 58.96

Gene sequence:

>1596_bases
ATGACAGGTAAACTTATTGCCACCGCCGTATATCTGGGTTGCTGTTTCTGGCTTTCCTGGAAGGCTTGGAAAGAAACAGG
AAGCAATGCCGACTACATGCTGGCCGGCAGACAGGCCGGGCCTTTTGTCATGGCCATGTCTTACGGAGCCACATTCATTT
CCACGTCGGCCATCATCGGTTTCGGCGGGGCCGCGGCCATGTTCGGTTTTTCCGTACTGTGGCTCACGGTGCTCAACATC
GGTGTGGGCGTTTTCATTGCCATGGTGTTTTTCGGCAGACGCACCCGCCGCATGGGGCTGGCTCTCGATTCGCACACGTT
TCCGGAACTGCTGGGCAGACGATATGACTCGCGTTTCATTCAGGGGTTTTCCGGCAGCATCATTTTTCTGTTCATTCCGG
TTTACGCCGCAGCAGTTCTCATCGGCATTGCGCGGCTGGCCGAAGTGTCGCTGGGTGTGCCTTATGTGGCGGCACTGCTC
AGCGTCACGGCCATTCTGGCACTGTATGTCATCACCGGCGGCATCAAGGCCGTCATGTACACCGACGCATTTCAAGGCAC
ACTTATGGTGCTTATGATGCTGATGCTTGTGGGCTATACCTACTGGATGCTGGGCGGCGTCATTCCCGCGCACGAAACCC
TTTCGGACATGGCGCCGCTGATGCCCGAGAAACTGCGCGCCGGCGGCATGCTGGGGTGGACACAGGGCACCGTGACCGGC
TCGCCCCTGTGGCTGGTCATCTACACCACCATCGTTTACGGCGTGGGCGTGGGGGTACTGGCGCAACCGCAGCTTGCCGT
GCGCTACATGACTGTGCCCTCGGACAGAGAACTGCACAGGGCCGTACTGTTCGGCGGAGTGTTCATTCTGCTGATGACCG
GCGTGGCTTTCACGGTCGGCGCACTTTCCAACGCGGTGTTTTTTCAGAAATTCGGTAAAATTGCCATACAGATGGGCGGC
GGAAATGTGGACAAGATAATTCCCGCCTATGTGGAAACCATGATGCCCGGCTGGTTTTCAGGATTTTTTCTGCTGGCCAT
GATGGCCGCCGCCATGTCCACCATGTCGGGGCAATATCATACCGGCGGCACGGCGCTGGGGCACGACATCATCACACGCA
CTCTGGGCAGAAAGCAGCAGGGGCTTGCCGCCAACCGCATCGGTGTGGTCATAACCATACTTGCCTCGCTGGTCTGGGCA
TGGGTACTGCCCGGCTCTGTCATTGCCAGAGCCACCGCTTTTTTCTTCGGGCTGTGCGCAGCCACTTTTCTGCCTGCCTA
TTCTCTGGGGCTTTACTGGCGCGGCATGACCAAAACCGGCGCCAAGGTGTCCATGCTGGGCGGATTCGCAGCATCCATGT
TCTGGCTGCTGTTCATCCATGAAAAAGAAGCCGCAGCCATCGGGCTGTGCCGCTGGCTGACCGGCAAAGCCACTCTGGTT
GCCGACGCAGTGCCCGGTTCGGCACTGCATCTGCTGCAGTGGGTAGACCCCAATGTGGTGGCGCTGCCGCTGTCGTTTCT
GCTGGCGGCGGGCGTCAGTACGGCTACCCGTCCGGTGGCACGGGAACATCTGGACCTGTGTTACAGCAGGTGCTGA

Upstream 100 bases:

>100_bases
GGCTGCGTAGGCTGGGGCATATGGAACTGGAACGAAAAAGGCTCTTCCGATGCAGGGCACCTGCCCCGCATGGATACCGG
CAAAGCCGGAGGGACAGAGA

Downstream 100 bases:

>100_bases
CGGTCCGGCACATCTGCCGGTACAATGCAGTGCCGTTATCGCCTCTGTCCGGCGTGCCGCGTTTTTCCGCGGCCGCCGGA
CAGCCGTTTCAGGACTTTTT

Product: sodium/proline symporter permease

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTGKLIATAVYLGCCFWLSWKAWKETGSNADYMLAGRQAGPFVMAMSYGATFISTSAIIGFGGAAAMFGFSVLWLTVLNI
GVGVFIAMVFFGRRTRRMGLALDSHTFPELLGRRYDSRFIQGFSGSIIFLFIPVYAAAVLIGIARLAEVSLGVPYVAALL
SVTAILALYVITGGIKAVMYTDAFQGTLMVLMMLMLVGYTYWMLGGVIPAHETLSDMAPLMPEKLRAGGMLGWTQGTVTG
SPLWLVIYTTIVYGVGVGVLAQPQLAVRYMTVPSDRELHRAVLFGGVFILLMTGVAFTVGALSNAVFFQKFGKIAIQMGG
GNVDKIIPAYVETMMPGWFSGFFLLAMMAAAMSTMSGQYHTGGTALGHDIITRTLGRKQQGLAANRIGVVITILASLVWA
WVLPGSVIARATAFFFGLCAATFLPAYSLGLYWRGMTKTGAKVSMLGGFAASMFWLLFIHEKEAAAIGLCRWLTGKATLV
ADAVPGSALHLLQWVDPNVVALPLSFLLAAGVSTATRPVAREHLDLCYSRC

Sequences:

>Translated_531_residues
MTGKLIATAVYLGCCFWLSWKAWKETGSNADYMLAGRQAGPFVMAMSYGATFISTSAIIGFGGAAAMFGFSVLWLTVLNI
GVGVFIAMVFFGRRTRRMGLALDSHTFPELLGRRYDSRFIQGFSGSIIFLFIPVYAAAVLIGIARLAEVSLGVPYVAALL
SVTAILALYVITGGIKAVMYTDAFQGTLMVLMMLMLVGYTYWMLGGVIPAHETLSDMAPLMPEKLRAGGMLGWTQGTVTG
SPLWLVIYTTIVYGVGVGVLAQPQLAVRYMTVPSDRELHRAVLFGGVFILLMTGVAFTVGALSNAVFFQKFGKIAIQMGG
GNVDKIIPAYVETMMPGWFSGFFLLAMMAAAMSTMSGQYHTGGTALGHDIITRTLGRKQQGLAANRIGVVITILASLVWA
WVLPGSVIARATAFFFGLCAATFLPAYSLGLYWRGMTKTGAKVSMLGGFAASMFWLLFIHEKEAAAIGLCRWLTGKATLV
ADAVPGSALHLLQWVDPNVVALPLSFLLAAGVSTATRPVAREHLDLCYSRC
>Mature_530_residues
TGKLIATAVYLGCCFWLSWKAWKETGSNADYMLAGRQAGPFVMAMSYGATFISTSAIIGFGGAAAMFGFSVLWLTVLNIG
VGVFIAMVFFGRRTRRMGLALDSHTFPELLGRRYDSRFIQGFSGSIIFLFIPVYAAAVLIGIARLAEVSLGVPYVAALLS
VTAILALYVITGGIKAVMYTDAFQGTLMVLMMLMLVGYTYWMLGGVIPAHETLSDMAPLMPEKLRAGGMLGWTQGTVTGS
PLWLVIYTTIVYGVGVGVLAQPQLAVRYMTVPSDRELHRAVLFGGVFILLMTGVAFTVGALSNAVFFQKFGKIAIQMGGG
NVDKIIPAYVETMMPGWFSGFFLLAMMAAAMSTMSGQYHTGGTALGHDIITRTLGRKQQGLAANRIGVVITILASLVWAW
VLPGSVIARATAFFFGLCAATFLPAYSLGLYWRGMTKTGAKVSMLGGFAASMFWLLFIHEKEAAAIGLCRWLTGKATLVA
DAVPGSALHLLQWVDPNVVALPLSFLLAAGVSTATRPVAREHLDLCYSRC

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=468, Percent_Identity=27.5641025641026, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI4507031, Length=465, Percent_Identity=21.9354838709677, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI87082237, Length=447, Percent_Identity=27.2930648769575, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1787251, Length=463, Percent_Identity=25.7019438444924, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1790113, Length=401, Percent_Identity=26.4339152119701, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790503, Length=464, Percent_Identity=22.8448275862069, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533094, Length=454, Percent_Identity=24.6696035242291, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 56952; Mature: 56821

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGKLIATAVYLGCCFWLSWKAWKETGSNADYMLAGRQAGPFVMAMSYGATFISTSAIIG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHC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EECCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]