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The map label for this gene is hypB [H]

Identifier: 78356354

GI number: 78356354

Start: 1326731

End: 1327390

Strand: Reverse

Name: hypB [H]

Synonym: Dde_1307

Alternate gene names: 78356354

Gene position: 1327390-1326731 (Counterclockwise)

Preceding gene: 78356355

Following gene: 78356353

Centisome position: 35.58

GC content: 55.91

Gene sequence:

>660_bases
ATGGAAATTCCCGTTATCCGCAACGTTCTGGAAGCCAACGAACAGACCGCGGCACAGCTGCGCAAACTGTTTGCTCAAAG
CGATCTGCTGGTTCTGAACCTTATCAGTTCACCCGGCGCGGGCAAAACCAGCCTGCTGGAACGCACTCTTACCGATCTGA
AGGACGAATTCGTCATGGCCGTGGTGGAGGGCGACCTGCAGACATCCAACGATGCGCGGCGCGTTGCGGCCACCGGAGCA
AAGGCAGTACAGATAAACACCGACGGCGGCTGCCATCTGAACAGCGCCATGGTGCTGGATGCCGTACAGCATTTCGACCT
GAAAGCAACGGATATCCTGTTCATCGAAAACGTGGGAAATCTGGTATGTCCCGTGGAATTTGACTGCGGCGAAGATTTTA
AAATCGCCCTGCTCAGCGTGACGGAAGGTGACGACAAGCCGGAAAAATACCCTCTGCTGTTCAACCTTTCCGCAGCCATG
GTGCTGAATAAGACCGACCTGCTGCCTTATGTCGATTTTGACGTGGAACGCGCACGCCGGTTTGCCACTCACCTTAATAA
GGACCTTGCTTTCCTGCCTGTTTCATGCCGCTCCGGCGAAGGGCTGCAAGCATGGTACGACTGGATACGCACAGCCGTAA
CCGCCAAGCGCGCGGCCTGA

Upstream 100 bases:

>100_bases
GTACATGCCCTGCCCCCGCTGCGGGCGCGAGTTTGCCCACGAGGTGCTGACAGGGCGCGAGCTTTTTGTCGATCACATCG
AAGCAGAATAAGGAAGCGCC

Downstream 100 bases:

>100_bases
TTTTCCGTATATGCACCGGCCCGCAACGCACCGCGCTGCGGGCTTTTTTTCGGGCAGCACATTCCGCCGCGCCACAACTT
TTTTCAGGAAGCATAAAGCC

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MEIPVIRNVLEANEQTAAQLRKLFAQSDLLVLNLISSPGAGKTSLLERTLTDLKDEFVMAVVEGDLQTSNDARRVAATGA
KAVQINTDGGCHLNSAMVLDAVQHFDLKATDILFIENVGNLVCPVEFDCGEDFKIALLSVTEGDDKPEKYPLLFNLSAAM
VLNKTDLLPYVDFDVERARRFATHLNKDLAFLPVSCRSGEGLQAWYDWIRTAVTAKRAA

Sequences:

>Translated_219_residues
MEIPVIRNVLEANEQTAAQLRKLFAQSDLLVLNLISSPGAGKTSLLERTLTDLKDEFVMAVVEGDLQTSNDARRVAATGA
KAVQINTDGGCHLNSAMVLDAVQHFDLKATDILFIENVGNLVCPVEFDCGEDFKIALLSVTEGDDKPEKYPLLFNLSAAM
VLNKTDLLPYVDFDVERARRFATHLNKDLAFLPVSCRSGEGLQAWYDWIRTAVTAKRAA
>Mature_219_residues
MEIPVIRNVLEANEQTAAQLRKLFAQSDLLVLNLISSPGAGKTSLLERTLTDLKDEFVMAVVEGDLQTSNDARRVAATGA
KAVQINTDGGCHLNSAMVLDAVQHFDLKATDILFIENVGNLVCPVEFDCGEDFKIALLSVTEGDDKPEKYPLLFNLSAAM
VLNKTDLLPYVDFDVERARRFATHLNKDLAFLPVSCRSGEGLQAWYDWIRTAVTAKRAA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=204, Percent_Identity=52.4509803921569, Blast_Score=220, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 24071; Mature: 24071

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPVIRNVLEANEQTAAQLRKLFAQSDLLVLNLISSPGAGKTSLLERTLTDLKDEFVMA
CCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEE
VVEGDLQTSNDARRVAATGAKAVQINTDGGCHLNSAMVLDAVQHFDLKATDILFIENVGN
EEECCCCCCCCHHHHEECCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCEEEEEECCCC
LVCPVEFDCGEDFKIALLSVTEGDDKPEKYPLLFNLSAAMVLNKTDLLPYVDFDVERARR
EEEEEECCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCEEECCCCCCEEECCCHHHHHH
FATHLNKDLAFLPVSCRSGEGLQAWYDWIRTAVTAKRAA
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIPVIRNVLEANEQTAAQLRKLFAQSDLLVLNLISSPGAGKTSLLERTLTDLKDEFVMA
CCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEE
VVEGDLQTSNDARRVAATGAKAVQINTDGGCHLNSAMVLDAVQHFDLKATDILFIENVGN
EEECCCCCCCCHHHHEECCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCEEEEEECCCC
LVCPVEFDCGEDFKIALLSVTEGDDKPEKYPLLFNLSAAMVLNKTDLLPYVDFDVERARR
EEEEEECCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCEEECCCCCCEEECCCHHHHHH
FATHLNKDLAFLPVSCRSGEGLQAWYDWIRTAVTAKRAA
HHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]