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The map label for this gene is ycdT [H]

Identifier: 78356218

GI number: 78356218

Start: 1195051

End: 1197075

Strand: Reverse

Name: ycdT [H]

Synonym: Dde_1171

Alternate gene names: 78356218

Gene position: 1197075-1195051 (Counterclockwise)

Preceding gene: 78356219

Following gene: 78356217

Centisome position: 32.09

GC content: 58.52

Gene sequence:

>2025_bases
ATGACTGTCCCGCGCTGCCGGCGTTTTTTTCTCACCCTTTCCGGGCTTGCCGCATGGGCCGTTCTGTGCCTGTGCACTAC
GCTGCTGCAGGCGCAGCCTGTCCGGGCATCGGGCCTGCAGCCGGTTACGCTGCAACTGCACTGGACGCATGAATTCGAGT
TTGCCGGTTTTTATGTTGCCAAAGAAAAAGGCTTTTATGCGGAACGCGGACTGGATGTGACCATAATACCCGGCGGGCCG
GGCATAGTGCCGGTGCAGGAGGTGCTGCGCGGCAATGCCCAGTACGGCATAGGTGCCGCCGAAGTGCTGCTGGCAAGGCT
GCGCGGTGCACCGCTGGTCATGCTGGCCGCAGTGTTTCAGCACTCTGCCAGCTGTATTCTTACCCGGGCGGATTCCGGCA
TCTACACCCCTCAGGACCTTGTGGGCAGAGTCTTTGAAATGGGCAGGCTGGAAAGCGACGCCGAAACCTATGCCATGCTG
CATAACGAAGGCATCGACATCAGCCAGCTGACCCACGTGGATTCGACATTCAATCCCCAGCTGCTGCTGGACGGCAAGGT
TGACGCCATTTCGGCATATTCTTTCAGCCAGCCTTTTTTTCTGCGCGAGCGGGGCATTCCCTACCGGCTCATCCGGCCCA
TCCAGTATGGTATCGATTTCTACGGCAATTCCATTTTTTCTTCAGAAAACGAAGTGCGCAGGCACCCCGAACGCACAGAG
GCATTCATAGAAGCCAGCATGAAAGGCTGGGCCTACGCCTTTGACCATGTGGACGAAGCCGTGGACATCATTCTGAACAA
ATACAGCGATCACCCCTTTGCCCACTCGCGCGAGCATCTGCTGTTCGAAGCGGAAGAAAGCCGCAAACTGGTTATGCCGC
ATCTGGTCGATCTGGGGCACATGAATCCCGGCCGCTGGAAACACATGGCCGACACGCTGGTACAGCTGGGGCTGGCTGAC
GCCGATTATACGCTGAAAGGCTTTCTTCATGATCCTGATCTGTCGTCCAGCCTGCTCGCCCACTGGGGTGTGCGGGCCGG
CATAGGCATTCTGGCGGGAGCCGTAGGACTGTGCCTTGTTCTGCTGCGCTTCAACCGCAGGCTTACCGCGGAAATCGCCT
CACGCCGCAAAGCCGAGGAACGGACGCTGAGAAGCGAACAACGGCTTTCGCTGGCGTTGTGGGCGGGCAATCTGGGCTGG
TGGGACTGGGACCTGAAATCCGACCATGTATCGCTGGACAGAACAGCCCACACCATGCTGGGCGGCAGCGACACCCCGCT
GCAGACGGCCCTGGAATCCATGGCCGGCAACCGCTCTCTTACAGGTGCCATGCCCTTTGTACATACGCTGCGCAACATGC
AGCAAAACGACACCCGCACCTTTGAAGAAGAAATGCAGCTTGATACAGGCGGAAAAAAACGCTGGGTGCTGTGCCGGGGA
CAGATGATCAACAGTTCCGGCCGCGCCGCCGGTGTTCTCATGGACATCAGCAAACTGAAGTCATACCAGCAGGAACTGGA
ACAGATGACCATGACCGACTATCTGACGGGACTCTTCAACCGCAGGCATTTCTTCCGTCAGCTGGAAACACATCTGGAGC
AGTACAAACGCAGGCAGTACAATCTCTGCGTGGCGCTGCTAGACCTTGACCACTTCAAGCGGATAAATGACACATACGGA
CACATGGCCGGCGACTCAGTCATCCGCGAGTTTGCCCTCCTGCTGAAGGAATCGCTGAGGCCGTACGATGTCGTTGCCCG
CTTCGGGGGCGAAGAATTCATCATCCTGCTGCTGGACAGCGGACGGGAAGAAGCGCTGGGCATTCTGGACAGGCTGCTGA
AAAAGGTTTCGGGACACACCTTTGACGCCGAAGGACATCATCTGAGGGTCACCTTCAGCGGCGGAATTGCCGAAGCCGAC
GAGCTGCCCGCCTCGGAACTGACCCCGAAGCGCATCGTGGCCGCCGCCGACCAGCGGCTGTATCTGGCCAAAACAGCAGG
CCGCAACACCATCCGCATCCGCTAG

Upstream 100 bases:

>100_bases
TGTCCGGTTATCACAGCTAATCACCGTACTTAATTGCAGGCGGATTCAACGGGGCACCGCGCACAGCCGCCCTGCTGCAT
CAGGGAGAATACTCAAGGCC

Downstream 100 bases:

>100_bases
CCGCCGCTGCGTCTTTTACGCCGTCATGGTGATATCCGTGGTTACCTGCGGCATACTCACGCACTTTTAATTCAGTACTT
GCCAGAAAGCGGGCCGCATA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MTVPRCRRFFLTLSGLAAWAVLCLCTTLLQAQPVRASGLQPVTLQLHWTHEFEFAGFYVAKEKGFYAERGLDVTIIPGGP
GIVPVQEVLRGNAQYGIGAAEVLLARLRGAPLVMLAAVFQHSASCILTRADSGIYTPQDLVGRVFEMGRLESDAETYAML
HNEGIDISQLTHVDSTFNPQLLLDGKVDAISAYSFSQPFFLRERGIPYRLIRPIQYGIDFYGNSIFSSENEVRRHPERTE
AFIEASMKGWAYAFDHVDEAVDIILNKYSDHPFAHSREHLLFEAEESRKLVMPHLVDLGHMNPGRWKHMADTLVQLGLAD
ADYTLKGFLHDPDLSSSLLAHWGVRAGIGILAGAVGLCLVLLRFNRRLTAEIASRRKAEERTLRSEQRLSLALWAGNLGW
WDWDLKSDHVSLDRTAHTMLGGSDTPLQTALESMAGNRSLTGAMPFVHTLRNMQQNDTRTFEEEMQLDTGGKKRWVLCRG
QMINSSGRAAGVLMDISKLKSYQQELEQMTMTDYLTGLFNRRHFFRQLETHLEQYKRRQYNLCVALLDLDHFKRINDTYG
HMAGDSVIREFALLLKESLRPYDVVARFGGEEFIILLLDSGREEALGILDRLLKKVSGHTFDAEGHHLRVTFSGGIAEAD
ELPASELTPKRIVAAADQRLYLAKTAGRNTIRIR

Sequences:

>Translated_674_residues
MTVPRCRRFFLTLSGLAAWAVLCLCTTLLQAQPVRASGLQPVTLQLHWTHEFEFAGFYVAKEKGFYAERGLDVTIIPGGP
GIVPVQEVLRGNAQYGIGAAEVLLARLRGAPLVMLAAVFQHSASCILTRADSGIYTPQDLVGRVFEMGRLESDAETYAML
HNEGIDISQLTHVDSTFNPQLLLDGKVDAISAYSFSQPFFLRERGIPYRLIRPIQYGIDFYGNSIFSSENEVRRHPERTE
AFIEASMKGWAYAFDHVDEAVDIILNKYSDHPFAHSREHLLFEAEESRKLVMPHLVDLGHMNPGRWKHMADTLVQLGLAD
ADYTLKGFLHDPDLSSSLLAHWGVRAGIGILAGAVGLCLVLLRFNRRLTAEIASRRKAEERTLRSEQRLSLALWAGNLGW
WDWDLKSDHVSLDRTAHTMLGGSDTPLQTALESMAGNRSLTGAMPFVHTLRNMQQNDTRTFEEEMQLDTGGKKRWVLCRG
QMINSSGRAAGVLMDISKLKSYQQELEQMTMTDYLTGLFNRRHFFRQLETHLEQYKRRQYNLCVALLDLDHFKRINDTYG
HMAGDSVIREFALLLKESLRPYDVVARFGGEEFIILLLDSGREEALGILDRLLKKVSGHTFDAEGHHLRVTFSGGIAEAD
ELPASELTPKRIVAAADQRLYLAKTAGRNTIRIR
>Mature_673_residues
TVPRCRRFFLTLSGLAAWAVLCLCTTLLQAQPVRASGLQPVTLQLHWTHEFEFAGFYVAKEKGFYAERGLDVTIIPGGPG
IVPVQEVLRGNAQYGIGAAEVLLARLRGAPLVMLAAVFQHSASCILTRADSGIYTPQDLVGRVFEMGRLESDAETYAMLH
NEGIDISQLTHVDSTFNPQLLLDGKVDAISAYSFSQPFFLRERGIPYRLIRPIQYGIDFYGNSIFSSENEVRRHPERTEA
FIEASMKGWAYAFDHVDEAVDIILNKYSDHPFAHSREHLLFEAEESRKLVMPHLVDLGHMNPGRWKHMADTLVQLGLADA
DYTLKGFLHDPDLSSSLLAHWGVRAGIGILAGAVGLCLVLLRFNRRLTAEIASRRKAEERTLRSEQRLSLALWAGNLGWW
DWDLKSDHVSLDRTAHTMLGGSDTPLQTALESMAGNRSLTGAMPFVHTLRNMQQNDTRTFEEEMQLDTGGKKRWVLCRGQ
MINSSGRAAGVLMDISKLKSYQQELEQMTMTDYLTGLFNRRHFFRQLETHLEQYKRRQYNLCVALLDLDHFKRINDTYGH
MAGDSVIREFALLLKESLRPYDVVARFGGEEFIILLLDSGREEALGILDRLLKKVSGHTFDAEGHHLRVTFSGGIAEADE
LPASELTPKRIVAAADQRLYLAKTAGRNTIRIR

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=164, Percent_Identity=39.0243902439024, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=35.5932203389831, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788381, Length=182, Percent_Identity=34.0659340659341, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1786584, Length=178, Percent_Identity=30.8988764044944, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=36.9426751592357, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=33.9506172839506, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081881, Length=254, Percent_Identity=29.1338582677165, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788085, Length=210, Percent_Identity=29.5238095238095, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=161, Percent_Identity=32.2981366459627, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1787541, Length=213, Percent_Identity=30.0469483568075, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787802, Length=182, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787056, Length=179, Percent_Identity=32.9608938547486, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081977, Length=147, Percent_Identity=29.2517006802721, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=168, Percent_Identity=32.1428571428571, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 75874; Mature: 75743

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPRCRRFFLTLSGLAAWAVLCLCTTLLQAQPVRASGLQPVTLQLHWTHEFEFAGFYVA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEEEEEEE
KEKGFYAERGLDVTIIPGGPGIVPVQEVLRGNAQYGIGAAEVLLARLRGAPLVMLAAVFQ
ECCCCHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
HSASCILTRADSGIYTPQDLVGRVFEMGRLESDAETYAMLHNEGIDISQLTHVDSTFNPQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCC
LLLDGKVDAISAYSFSQPFFLRERGIPYRLIRPIQYGIDFYGNSIFSSENEVRRHPERTE
EEECCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHH
AFIEASMKGWAYAFDHVDEAVDIILNKYSDHPFAHSREHLLFEAEESRKLVMPHLVDLGH
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCC
MNPGRWKHMADTLVQLGLADADYTLKGFLHDPDLSSSLLAHWGVRAGIGILAGAVGLCLV
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
LLRFNRRLTAEIASRRKAEERTLRSEQRLSLALWAGNLGWWDWDLKSDHVSLDRTAHTML
HHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEECCCCCCCEEHHHHHHHHC
GGSDTPLQTALESMAGNRSLTGAMPFVHTLRNMQQNDTRTFEEEMQLDTGGKKRWVLCRG
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
QMINSSGRAAGVLMDISKLKSYQQELEQMTMTDYLTGLFNRRHFFRQLETHLEQYKRRQY
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLCVALLDLDHFKRINDTYGHMAGDSVIREFALLLKESLRPYDVVARFGGEEFIILLLDS
HHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECC
GREEALGILDRLLKKVSGHTFDAEGHHLRVTFSGGIAEADELPASELTPKRIVAAADQRL
CCHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHCCCEE
YLAKTAGRNTIRIR
EEEECCCCCEEEEC
>Mature Secondary Structure 
TVPRCRRFFLTLSGLAAWAVLCLCTTLLQAQPVRASGLQPVTLQLHWTHEFEFAGFYVA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEEEEEEE
KEKGFYAERGLDVTIIPGGPGIVPVQEVLRGNAQYGIGAAEVLLARLRGAPLVMLAAVFQ
ECCCCHHHCCCEEEEECCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
HSASCILTRADSGIYTPQDLVGRVFEMGRLESDAETYAMLHNEGIDISQLTHVDSTFNPQ
CCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHCCCCCCCCC
LLLDGKVDAISAYSFSQPFFLRERGIPYRLIRPIQYGIDFYGNSIFSSENEVRRHPERTE
EEECCCCCEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHH
AFIEASMKGWAYAFDHVDEAVDIILNKYSDHPFAHSREHLLFEAEESRKLVMPHLVDLGH
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCC
MNPGRWKHMADTLVQLGLADADYTLKGFLHDPDLSSSLLAHWGVRAGIGILAGAVGLCLV
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHH
LLRFNRRLTAEIASRRKAEERTLRSEQRLSLALWAGNLGWWDWDLKSDHVSLDRTAHTML
HHHHCCHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEECCCCCCCEEHHHHHHHHC
GGSDTPLQTALESMAGNRSLTGAMPFVHTLRNMQQNDTRTFEEEMQLDTGGKKRWVLCRG
CCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEEC
QMINSSGRAAGVLMDISKLKSYQQELEQMTMTDYLTGLFNRRHFFRQLETHLEQYKRRQY
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLCVALLDLDHFKRINDTYGHMAGDSVIREFALLLKESLRPYDVVARFGGEEFIILLLDS
HHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEECC
GREEALGILDRLLKKVSGHTFDAEGHHLRVTFSGGIAEADELPASELTPKRIVAAADQRL
CCHHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCHHCCCCCCCCHHHHHHHCCCEE
YLAKTAGRNTIRIR
EEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]