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The map label for this gene is murD

Identifier: 78356087

GI number: 78356087

Start: 1067852

End: 1069195

Strand: Direct

Name: murD

Synonym: Dde_1040

Alternate gene names: 78356087

Gene position: 1067852-1069195 (Clockwise)

Preceding gene: 78356086

Following gene: 78356088

Centisome position: 28.63

GC content: 61.01

Gene sequence:

>1344_bases
ATGCATACCGTTATACCGCATACACACGACACGCCTGCCGCCGCACCGGTACGTTCCGGCGCTGTGGCCGTGGTGGTGGG
CACCGGCCGTTCCGGCACGGCTGCCGCACGTCTGCTGCACCATCTCGGGGCGTCAGTCCGCATAGTCGAAAAAGACGAAA
GCCGTGTTTCCGCCGAATTCGCCTCGCTGGCGGCTAGACTGGGATTTGACTGCCGCTACGGCAGCCACAGCGCAGAACAG
TTTGCAGGTGCCGATATTCTGGTACCCAGCCCCGGCGTGCCGGTGGCATCACTGCTGCCGTATCTTGATGCCTCCCGCCC
GCCGGAAATTATGGCCGAAATTGATCTGGCTGCGCGCTGCGTGACCAGTCCGGTGCTGGCTGTTACCGGCACAAGCGGCA
AAACCACAACCGTCAGCCTGTGCGCGGCTATGCTGCGTGCAGCGGGCAAGCGTGTTTTTCTGGGGGGCAACATAGGCACG
CCCCTGTCGGAATATGTGCTGGATGTGGCTGCTGGTGAGGCTCCCGCCGATGTGCTGGTGCTGGAAGTCAGCAGTTTTCA
GCTGCAGACATGCACCACACTGCGGCCCGAAGTGGCGGTGCTCATAAATATTTCTGCCAACCACCTCGATTACCATGCTG
ACATGCAGGAGTACCTTGATGCCAAGTTCCGGCTGTTTGCCCTGCAGGGGCCGGATGATCTGGCCGTGCTGCAAAGCGGC
ATGGAGGAACTTGCCGACCGCTATGCGCTGGCGGCCCGCCGCGAGTTTTTCGATGCTGTGCGGCGTTTTCCGCGCACGGC
GCTGCTGGGCAGTCATAACATGGCCAACATAGAGGCAGCCTGGCTTGCCTGCCGGGCTTTCGGCGTGACGCAGTCGCAGG
CGGCGCAGGCGGTGGAAGAGTTTGCCCCGCTGGAGCACCGGCTGGAAAAGGTGGACGAGGTGGCCGGCGTGCTTTTTGTC
AATGATTCAAAATCAACCACGGTGGATTCCATGAAGGCGGCGCTGGAAAGTTTTGACAGGCCCGTATTGCTGCTGTGCGG
CGGCAAATGGAAGGGAGGGGATCTTGTATCGCTGATTCCCGTCGTCCGCCGTCATGCAAAAGCCGTGGGGCTGTTCGGCG
CCAGCAGAGAAATTTTTGAACACGCGTGGGCGGATGTGGTGCCCATGAGCTGGGATCATGACCTGCAGACGGCGTTCAGA
CGTATGACGGCCATGGCACAGCCGGGCGATGTGGTGCTTATGTCGCCTGCCACGTCCAGTTATGACCTGTACAGCAACTA
CAAGGAACGCGGCGGAGATTTCAAGCGGAATGTGGCCGCGTGGAAAGCGGAGCGTGACGACTGA

Upstream 100 bases:

>100_bases
TTCCCGAGTCAAAAATCATTATCCGCTTCTGGATAATGTCCATCGTGTTCGGACTGATGGCGCTTTCGGTGCTCAAGCTG
CGCTGAAGGGGAAGACAGAT

Downstream 100 bases:

>100_bases
TGGCAAGGGCGCAGACGGCAGAAGGCATCCGCACGGCCGACTGGTGGCTGCTTGTGGCGGCACTGGCACTGCTGGGCTTC
GGGCTTATGATGGTGCTGTC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MHTVIPHTHDTPAAAPVRSGAVAVVVGTGRSGTAAARLLHHLGASVRIVEKDESRVSAEFASLAARLGFDCRYGSHSAEQ
FAGADILVPSPGVPVASLLPYLDASRPPEIMAEIDLAARCVTSPVLAVTGTSGKTTTVSLCAAMLRAAGKRVFLGGNIGT
PLSEYVLDVAAGEAPADVLVLEVSSFQLQTCTTLRPEVAVLINISANHLDYHADMQEYLDAKFRLFALQGPDDLAVLQSG
MEELADRYALAARREFFDAVRRFPRTALLGSHNMANIEAAWLACRAFGVTQSQAAQAVEEFAPLEHRLEKVDEVAGVLFV
NDSKSTTVDSMKAALESFDRPVLLLCGGKWKGGDLVSLIPVVRRHAKAVGLFGASREIFEHAWADVVPMSWDHDLQTAFR
RMTAMAQPGDVVLMSPATSSYDLYSNYKERGGDFKRNVAAWKAERDD

Sequences:

>Translated_447_residues
MHTVIPHTHDTPAAAPVRSGAVAVVVGTGRSGTAAARLLHHLGASVRIVEKDESRVSAEFASLAARLGFDCRYGSHSAEQ
FAGADILVPSPGVPVASLLPYLDASRPPEIMAEIDLAARCVTSPVLAVTGTSGKTTTVSLCAAMLRAAGKRVFLGGNIGT
PLSEYVLDVAAGEAPADVLVLEVSSFQLQTCTTLRPEVAVLINISANHLDYHADMQEYLDAKFRLFALQGPDDLAVLQSG
MEELADRYALAARREFFDAVRRFPRTALLGSHNMANIEAAWLACRAFGVTQSQAAQAVEEFAPLEHRLEKVDEVAGVLFV
NDSKSTTVDSMKAALESFDRPVLLLCGGKWKGGDLVSLIPVVRRHAKAVGLFGASREIFEHAWADVVPMSWDHDLQTAFR
RMTAMAQPGDVVLMSPATSSYDLYSNYKERGGDFKRNVAAWKAERDD
>Mature_447_residues
MHTVIPHTHDTPAAAPVRSGAVAVVVGTGRSGTAAARLLHHLGASVRIVEKDESRVSAEFASLAARLGFDCRYGSHSAEQ
FAGADILVPSPGVPVASLLPYLDASRPPEIMAEIDLAARCVTSPVLAVTGTSGKTTTVSLCAAMLRAAGKRVFLGGNIGT
PLSEYVLDVAAGEAPADVLVLEVSSFQLQTCTTLRPEVAVLINISANHLDYHADMQEYLDAKFRLFALQGPDDLAVLQSG
MEELADRYALAARREFFDAVRRFPRTALLGSHNMANIEAAWLACRAFGVTQSQAAQAVEEFAPLEHRLEKVDEVAGVLFV
NDSKSTTVDSMKAALESFDRPVLLLCGGKWKGGDLVSLIPVVRRHAKAVGLFGASREIFEHAWADVVPMSWDHDLQTAFR
RMTAMAQPGDVVLMSPATSSYDLYSNYKERGGDFKRNVAAWKAERDD

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=443, Percent_Identity=30.0225733634311, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_DESDG (Q313Q5)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_387536.1
- ProteinModelPortal:   Q313Q5
- SMR:   Q313Q5
- STRING:   Q313Q5
- GeneID:   3755504
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1040
- NMPDR:   fig|207559.3.peg.869
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK705829
- BioCyc:   DDES207559:DDE_1040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR002938
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF01494 FAD_binding_3; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 48089; Mature: 48089

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTVIPHTHDTPAAAPVRSGAVAVVVGTGRSGTAAARLLHHLGASVRIVEKDESRVSAEF
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
ASLAARLGFDCRYGSHSAEQFAGADILVPSPGVPVASLLPYLDASRPPEIMAEIDLAARC
HHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
VTSPVLAVTGTSGKTTTVSLCAAMLRAAGKRVFLGGNIGTPLSEYVLDVAAGEAPADVLV
HCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEE
LEVSSFQLQTCTTLRPEVAVLINISANHLDYHADMQEYLDAKFRLFALQGPDDLAVLQSG
EEECCCEEEHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
MEELADRYALAARREFFDAVRRFPRTALLGSHNMANIEAAWLACRAFGVTQSQAAQAVEE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
FAPLEHRLEKVDEVAGVLFVNDSKSTTVDSMKAALESFDRPVLLLCGGKWKGGDLVSLIP
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
VVRRHAKAVGLFGASREIFEHAWADVVPMSWDHDLQTAFRRMTAMAQPGDVVLMSPATSS
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
YDLYSNYKERGGDFKRNVAAWKAERDD
HHHHHHHHHCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MHTVIPHTHDTPAAAPVRSGAVAVVVGTGRSGTAAARLLHHLGASVRIVEKDESRVSAEF
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHH
ASLAARLGFDCRYGSHSAEQFAGADILVPSPGVPVASLLPYLDASRPPEIMAEIDLAARC
HHHHHHHCCCCCCCCCCHHHHCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
VTSPVLAVTGTSGKTTTVSLCAAMLRAAGKRVFLGGNIGTPLSEYVLDVAAGEAPADVLV
HCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCEEE
LEVSSFQLQTCTTLRPEVAVLINISANHLDYHADMQEYLDAKFRLFALQGPDDLAVLQSG
EEECCCEEEHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
MEELADRYALAARREFFDAVRRFPRTALLGSHNMANIEAAWLACRAFGVTQSQAAQAVEE
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
FAPLEHRLEKVDEVAGVLFVNDSKSTTVDSMKAALESFDRPVLLLCGGKWKGGDLVSLIP
HHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
VVRRHAKAVGLFGASREIFEHAWADVVPMSWDHDLQTAFRRMTAMAQPGDVVLMSPATSS
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
YDLYSNYKERGGDFKRNVAAWKAERDD
HHHHHHHHHCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA