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The map label for this gene is murE [H]

Identifier: 78356084

GI number: 78356084

Start: 1063894

End: 1065348

Strand: Direct

Name: murE [H]

Synonym: Dde_1037

Alternate gene names: 78356084

Gene position: 1063894-1065348 (Clockwise)

Preceding gene: 78356083

Following gene: 78356085

Centisome position: 28.52

GC content: 61.44

Gene sequence:

>1455_bases
ATGAAAAGCACACTCTCATTCGACGCGCTTGCCGCAGCGGTGCGCGCCGGACTGCTGAACATCCGTATTGATTCACGGAA
AGTACGGCAGGGCGATGTCTTTGTGGCCCTGCCGGGGTCTTCCGTGGACGGCGGGGCGTTTATTGCCGATGCGCTTGAGC
GCGGAGCGTCATATGTGGTCTGCAGACCCTGCAGCGTGCCGGTGCAGACCGGCGGCGCGGCCGTGGTCGTGGCCGAAGAC
CCGCGCAAGGCTCTGGGGCTGCTGGCTGCTGCACGCTATGATACTGCCGATACGGGGTTTCCGCTTGTGGGCATCACGGG
AACCAACGGCAAGACAACATGTACTTATCTGCTGGACCATCTTTTCCGCACTGCCGGGCACGTAACCGGTCTGCTGGGCA
CCGTGAATTTCCGCTGGCCGGGGCACGACGAAGCCGCGCCCATGACCACCCCCGACTGCGTTGAACTGCATTCCATGTTG
TCGGACATGCGCGATGCGGGCGTCGAAGCCGCCTTCATGGAAGTTTCATCACATGCACTCGACCAGCAGCGGGTTTCCGG
CGCTCGTTTTGCCGCGGCGCTGCTGACCAATTTAACGCAGGACCATCTGGATTATCACCACGATATGGAAGCCTATTTCA
GCGCAAAATCCAGACTGTTTCTTGAACTGCCAGACGAGGACAAAGTCTGCGCGGTGAATGCCGATGACCCGTACGGGCGC
AGGCTGCTGCCGCAACTGGGCCGCGGTGTGGGCTATTCGGTACAGGGTACTGTTGTGCCGGACTGTCAGGTGCTGCAGGC
CACCATACTTTCAAGCGGGCCCGATGGGCTGCATCTGTCCATGGATTTTGAAGGGAAGACATGGGAGCTGCGTTCGCCGC
TGGTGGGCGCGTTCAACGCAAGCAATCTTGCAGGGGTGCAGGCTGCGGGGCTGGCCATGGGGCTGCCCGTTGAGGCCATG
CAGGGGCTGGCAAGCTTTTCCGGTGTGCCCGGCCGCATGGAGCGCATAGAAAATGACAAGGGTCTCAATGTGTTTGTCGA
TTATGCCCACACGCCGGATGCACTGGAAAATGTACTCAGGGCGCTGCGGGCCGCAGGTTTTGCACGGGTCATAGCCGTGT
TCGGCTGCGGCGGCGACCGTGACCGTACCAAGCGTCCGCTTATGGGGCAGGCCGTGTGCCGTCATGCAGACGTGGCGGTA
CTGACTTCTGACAACCCGCGCACCGAAGACCCGGTGGCCATCATGGCCGATGTCAAACCCGGTCTGGCACAGTGCGACCA
TGTGGTGGAAGAGCCCGACAGACGCAAGGCTCTTGCCGCGGCGGTGGAAATGGCCCGCCCCGGCGATGCCGTGCTGGTGG
CCGGAAAGGGGCACGAGGATTATCAGGTGATCGGCACCACAAAGATACATTTCAGTGATCAGGAAACCCTGCGGGAGCTT
TTGGCGTGCGCATGA

Upstream 100 bases:

>100_bases
GGGGCACCGTTGTGCGGCAGCAGCCCGCGGCAGGGTCGCCGTGGCCGGATAATATAACAGAATCACAGTACGTACTGTGG
GTTATGGAGCAGTCCTGACC

Downstream 100 bases:

>100_bases
ATCTGGAACAGATACGCGACGCTCTGGGCGCCGTGGGAACGCTGGGCGGAGCGGACATGACCGTGCCTTCCGGTGTGCAG
ACCGACAGCCGGTGCGTGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKSTLSFDALAAAVRAGLLNIRIDSRKVRQGDVFVALPGSSVDGGAFIADALERGASYVVCRPCSVPVQTGGAAVVVAED
PRKALGLLAAARYDTADTGFPLVGITGTNGKTTCTYLLDHLFRTAGHVTGLLGTVNFRWPGHDEAAPMTTPDCVELHSML
SDMRDAGVEAAFMEVSSHALDQQRVSGARFAAALLTNLTQDHLDYHHDMEAYFSAKSRLFLELPDEDKVCAVNADDPYGR
RLLPQLGRGVGYSVQGTVVPDCQVLQATILSSGPDGLHLSMDFEGKTWELRSPLVGAFNASNLAGVQAAGLAMGLPVEAM
QGLASFSGVPGRMERIENDKGLNVFVDYAHTPDALENVLRALRAAGFARVIAVFGCGGDRDRTKRPLMGQAVCRHADVAV
LTSDNPRTEDPVAIMADVKPGLAQCDHVVEEPDRRKALAAAVEMARPGDAVLVAGKGHEDYQVIGTTKIHFSDQETLREL
LACA

Sequences:

>Translated_484_residues
MKSTLSFDALAAAVRAGLLNIRIDSRKVRQGDVFVALPGSSVDGGAFIADALERGASYVVCRPCSVPVQTGGAAVVVAED
PRKALGLLAAARYDTADTGFPLVGITGTNGKTTCTYLLDHLFRTAGHVTGLLGTVNFRWPGHDEAAPMTTPDCVELHSML
SDMRDAGVEAAFMEVSSHALDQQRVSGARFAAALLTNLTQDHLDYHHDMEAYFSAKSRLFLELPDEDKVCAVNADDPYGR
RLLPQLGRGVGYSVQGTVVPDCQVLQATILSSGPDGLHLSMDFEGKTWELRSPLVGAFNASNLAGVQAAGLAMGLPVEAM
QGLASFSGVPGRMERIENDKGLNVFVDYAHTPDALENVLRALRAAGFARVIAVFGCGGDRDRTKRPLMGQAVCRHADVAV
LTSDNPRTEDPVAIMADVKPGLAQCDHVVEEPDRRKALAAAVEMARPGDAVLVAGKGHEDYQVIGTTKIHFSDQETLREL
LACA
>Mature_484_residues
MKSTLSFDALAAAVRAGLLNIRIDSRKVRQGDVFVALPGSSVDGGAFIADALERGASYVVCRPCSVPVQTGGAAVVVAED
PRKALGLLAAARYDTADTGFPLVGITGTNGKTTCTYLLDHLFRTAGHVTGLLGTVNFRWPGHDEAAPMTTPDCVELHSML
SDMRDAGVEAAFMEVSSHALDQQRVSGARFAAALLTNLTQDHLDYHHDMEAYFSAKSRLFLELPDEDKVCAVNADDPYGR
RLLPQLGRGVGYSVQGTVVPDCQVLQATILSSGPDGLHLSMDFEGKTWELRSPLVGAFNASNLAGVQAAGLAMGLPVEAM
QGLASFSGVPGRMERIENDKGLNVFVDYAHTPDALENVLRALRAAGFARVIAVFGCGGDRDRTKRPLMGQAVCRHADVAV
LTSDNPRTEDPVAIMADVKPGLAQCDHVVEEPDRRKALAAAVEMARPGDAVLVAGKGHEDYQVIGTTKIHFSDQETLREL
LACA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=472, Percent_Identity=39.4067796610169, Blast_Score=267, Evalue=9e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 51492; Mature: 51492

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00485 A_DEAMINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTLSFDALAAAVRAGLLNIRIDSRKVRQGDVFVALPGSSVDGGAFIADALERGASYVV
CCCCCCHHHHHHHHHHCEEEEEECCCEECCCCEEEEECCCCCCCCHHHHHHHHCCCCEEE
CRPCSVPVQTGGAAVVVAEDPRKALGLLAAARYDTADTGFPLVGITGTNGKTTCTYLLDH
EECCCCCEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
LFRTAGHVTGLLGTVNFRWPGHDEAAPMTTPDCVELHSMLSDMRDAGVEAAFMEVSSHAL
HHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DQQRVSGARFAAALLTNLTQDHLDYHHDMEAYFSAKSRLFLELPDEDKVCAVNADDPYGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCHH
RLLPQLGRGVGYSVQGTVVPDCQVLQATILSSGPDGLHLSMDFEGKTWELRSPLVGAFNA
HHHHHHCCCCCCEECCEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCEECCCC
SNLAGVQAAGLAMGLPVEAMQGLASFSGVPGRMERIENDKGLNVFVDYAHTPDALENVLR
CCCCCHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
ALRAAGFARVIAVFGCGGDRDRTKRPLMGQAVCRHADVAVLTSDNPRTEDPVAIMADVKP
HHHHCCHHEEHEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCC
GLAQCDHVVEEPDRRKALAAAVEMARPGDAVLVAGKGHEDYQVIGTTKIHFSDQETLREL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCHHHHHHH
LACA
HHCC
>Mature Secondary Structure
MKSTLSFDALAAAVRAGLLNIRIDSRKVRQGDVFVALPGSSVDGGAFIADALERGASYVV
CCCCCCHHHHHHHHHHCEEEEEECCCEECCCCEEEEECCCCCCCCHHHHHHHHCCCCEEE
CRPCSVPVQTGGAAVVVAEDPRKALGLLAAARYDTADTGFPLVGITGTNGKTTCTYLLDH
EECCCCCEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
LFRTAGHVTGLLGTVNFRWPGHDEAAPMTTPDCVELHSMLSDMRDAGVEAAFMEVSSHAL
HHHHHHHHHEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
DQQRVSGARFAAALLTNLTQDHLDYHHDMEAYFSAKSRLFLELPDEDKVCAVNADDPYGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCHH
RLLPQLGRGVGYSVQGTVVPDCQVLQATILSSGPDGLHLSMDFEGKTWELRSPLVGAFNA
HHHHHHCCCCCCEECCEECCCHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCEECCCC
SNLAGVQAAGLAMGLPVEAMQGLASFSGVPGRMERIENDKGLNVFVDYAHTPDALENVLR
CCCCCHHHCCHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHH
ALRAAGFARVIAVFGCGGDRDRTKRPLMGQAVCRHADVAVLTSDNPRTEDPVAIMADVKP
HHHHCCHHEEHEEEECCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCC
GLAQCDHVVEEPDRRKALAAAVEMARPGDAVLVAGKGHEDYQVIGTTKIHFSDQETLREL
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEEEEEECCHHHHHHH
LACA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA