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The map label for this gene is cisA [H]

Identifier: 78355943

GI number: 78355943

Start: 923367

End: 924950

Strand: Direct

Name: cisA [H]

Synonym: Dde_0896

Alternate gene names: 78355943

Gene position: 923367-924950 (Clockwise)

Preceding gene: 78355942

Following gene: 78355944

Centisome position: 24.75

GC content: 54.36

Gene sequence:

>1584_bases
ATGCTTAACAATAACAACACACAAAACGGCCAGCGTAAAACGCTCCGCTGTGCCATCTATACCCGGAAAAGCCACGAGGA
AGGTCTGGATCAGGAATTCAATTCACTCGACGCCCAGAGGGAGGCCGCCGAAGCCTACATCGAAAGTCAGAAGCTGCAGG
GATGGAAGGCCATCCCTTACCGATATGATGACGGCGGCTTTTCCGGCGGCACCATGGAACGTCCGGCTCTGCAGAGACTG
CTGGCGGATATCGATGCCGGTAAGATCGATGTCATCGTTGTTTACAAGATCGACCGGCTATCCCGATCCCTGCTGGACTT
CATGAAGATGATTGAGCTTTTCAATGAAAAGGAAGTGAGCTTTGTATCCGTCACCCAGCACTTCAGCACCACTGACTCGA
CGGGCCGCATGTTTCTGGGAATCCTGATCACCTTTGCCCAGTATGAGCGTGAGGTCATTGGTGAGCGTATCCGGGACAAG
GTGGCCGCCGCTAAACGCCGGGGAAAATACTGCGGCGGGCCTGCTGTACTCGGTTACGACGTGGACCGGGAAAACAAAAA
GCTGCTCATCAACCAGAGCGAGGCACCGCTGGTGAAACTCATCTTCAGGCGATACACGCAGGTGGGATCGGCAAAGAAGG
TGGCGCAGGAGCTTAACGAGCAAGGATACAAAACCAAGTCATGGACCACCAAGAAAGGCAAGGAGCGGATCGGTACCGAA
TGGAACACGGCTCAGGTCTATCGCCTTCTCAACAACAGGCTCTATATCGGAGAGATCGCTTACAAGGGTAAAAACTATCC
GGCGGAGCATGATGCCCTGATTGATCAAAACACATGGGACAAGGTTCAGGCGCTGCTCTCGGAAAACAATCGAACCAAGA
TGAGCAAGGCCCGGGTGAAAATGGTCTCGCCCCTGAGCGGAGTGATCCGGTGCGGCCACTGCGACAGCGCCATGGGCATC
ACCTATACCAACAAAGGCGACCGCCGCTACTCCTATTATATATGTGAAAAGGATACCAAACGCGCCGTCAGCCGGTGCCC
TTTGAAACGGGTTCCTGCCGGAGACATCGAGTCGGTGGTACTTGACCAGCTGGGAGCCGTATTCAGAACACCGACCTTGG
TGGCCAAGACCTACTTTGCCGCCAGAGAGATCGAGGCCGAAGAACGAGAGCGCTTGCAGGTCCAGAAGAAGGAACTCGAA
CAATCCCTGCAGAGCGTCAGGCAGGAAGCTCTGAAGCTGATGTCGCCCGACAATGATGATCCCGACCGGAACAGCCGACT
GCCCTTGGTCAATCAGCAGGCCGTCGATCTGACCAAACAGCTCACCAATGTATCGGCCCGATTGAAGGTAATTGATACCG
AACAGATTTCCGAGGGGGATGTATCCGAAGCCTTTCAGAGCGTGAAAACCTTCTGGGAGGATCTGTTTCCCCTCGAGCGA
AACCGACTGATCCAGCTTCTGGTGGAAACCATCGAAATACGGGAAACGGGAATCGACATGGAACTGAAAACCAACGGTCT
CACAAACCTTGTCACCGAGCTGGCCGGTCTGGCCTGTGAAGTCAGGGAAAGGAGTAACAGCTGA

Upstream 100 bases:

>100_bases
TCACTGAGCGCCATAGCGAGGGAAATCACAGGCACCCGCTGGAACGGCAAGATCTTTTTCGGCCTGAAGAACAGTCACAG
AAAAAAGGAAGGTGGTCCGA

Downstream 100 bases:

>100_bases
TGAAGAAGATTAAACCAACCATCAAACTTTCAGACAGCGGAAACCTGCACATCCATATCCCCATGTTCATCCGGAGAATG
CGCGGCCGCAAAATGGTATT

Product: site-specific recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MLNNNNTQNGQRKTLRCAIYTRKSHEEGLDQEFNSLDAQREAAEAYIESQKLQGWKAIPYRYDDGGFSGGTMERPALQRL
LADIDAGKIDVIVVYKIDRLSRSLLDFMKMIELFNEKEVSFVSVTQHFSTTDSTGRMFLGILITFAQYEREVIGERIRDK
VAAAKRRGKYCGGPAVLGYDVDRENKKLLINQSEAPLVKLIFRRYTQVGSAKKVAQELNEQGYKTKSWTTKKGKERIGTE
WNTAQVYRLLNNRLYIGEIAYKGKNYPAEHDALIDQNTWDKVQALLSENNRTKMSKARVKMVSPLSGVIRCGHCDSAMGI
TYTNKGDRRYSYYICEKDTKRAVSRCPLKRVPAGDIESVVLDQLGAVFRTPTLVAKTYFAAREIEAEERERLQVQKKELE
QSLQSVRQEALKLMSPDNDDPDRNSRLPLVNQQAVDLTKQLTNVSARLKVIDTEQISEGDVSEAFQSVKTFWEDLFPLER
NRLIQLLVETIEIRETGIDMELKTNGLTNLVTELAGLACEVRERSNS

Sequences:

>Translated_527_residues
MLNNNNTQNGQRKTLRCAIYTRKSHEEGLDQEFNSLDAQREAAEAYIESQKLQGWKAIPYRYDDGGFSGGTMERPALQRL
LADIDAGKIDVIVVYKIDRLSRSLLDFMKMIELFNEKEVSFVSVTQHFSTTDSTGRMFLGILITFAQYEREVIGERIRDK
VAAAKRRGKYCGGPAVLGYDVDRENKKLLINQSEAPLVKLIFRRYTQVGSAKKVAQELNEQGYKTKSWTTKKGKERIGTE
WNTAQVYRLLNNRLYIGEIAYKGKNYPAEHDALIDQNTWDKVQALLSENNRTKMSKARVKMVSPLSGVIRCGHCDSAMGI
TYTNKGDRRYSYYICEKDTKRAVSRCPLKRVPAGDIESVVLDQLGAVFRTPTLVAKTYFAAREIEAEERERLQVQKKELE
QSLQSVRQEALKLMSPDNDDPDRNSRLPLVNQQAVDLTKQLTNVSARLKVIDTEQISEGDVSEAFQSVKTFWEDLFPLER
NRLIQLLVETIEIRETGIDMELKTNGLTNLVTELAGLACEVRERSNS
>Mature_527_residues
MLNNNNTQNGQRKTLRCAIYTRKSHEEGLDQEFNSLDAQREAAEAYIESQKLQGWKAIPYRYDDGGFSGGTMERPALQRL
LADIDAGKIDVIVVYKIDRLSRSLLDFMKMIELFNEKEVSFVSVTQHFSTTDSTGRMFLGILITFAQYEREVIGERIRDK
VAAAKRRGKYCGGPAVLGYDVDRENKKLLINQSEAPLVKLIFRRYTQVGSAKKVAQELNEQGYKTKSWTTKKGKERIGTE
WNTAQVYRLLNNRLYIGEIAYKGKNYPAEHDALIDQNTWDKVQALLSENNRTKMSKARVKMVSPLSGVIRCGHCDSAMGI
TYTNKGDRRYSYYICEKDTKRAVSRCPLKRVPAGDIESVVLDQLGAVFRTPTLVAKTYFAAREIEAEERERLQVQKKELE
QSLQSVRQEALKLMSPDNDDPDRNSRLPLVNQQAVDLTKQLTNVSARLKVIDTEQISEGDVSEAFQSVKTFWEDLFPLER
NRLIQLLVETIEIRETGIDMELKTNGLTNLVTELAGLACEVRERSNS

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=106, Percent_Identity=35.8490566037736, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787827, Length=148, Percent_Identity=31.0810810810811, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787638, Length=210, Percent_Identity=27.6190476190476, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 60058; Mature: 60058

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNNNTQNGQRKTLRCAIYTRKSHEEGLDQEFNSLDAQREAAEAYIESQKLQGWKAIPY
CCCCCCCCCCCHHEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCE
RYDDGGFSGGTMERPALQRLLADIDAGKIDVIVVYKIDRLSRSLLDFMKMIELFNEKEVS
EECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCE
FVSVTQHFSTTDSTGRMFLGILITFAQYEREVIGERIRDKVAAAKRRGKYCGGPAVLGYD
EEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
VDRENKKLLINQSEAPLVKLIFRRYTQVGSAKKVAQELNEQGYKTKSWTTKKGKERIGTE
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC
WNTAQVYRLLNNRLYIGEIAYKGKNYPAEHDALIDQNTWDKVQALLSENNRTKMSKARVK
CCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
MVSPLSGVIRCGHCDSAMGITYTNKGDRRYSYYICEKDTKRAVSRCPLKRVPAGDIESVV
HHCHHHHHEECCCCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCHHHHH
LDQLGAVFRTPTLVAKTYFAAREIEAEERERLQVQKKELEQSLQSVRQEALKLMSPDNDD
HHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDRNSRLPLVNQQAVDLTKQLTNVSARLKVIDTEQISEGDVSEAFQSVKTFWEDLFPLER
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHH
NRLIQLLVETIEIRETGIDMELKTNGLTNLVTELAGLACEVRERSNS
HHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNNNNTQNGQRKTLRCAIYTRKSHEEGLDQEFNSLDAQREAAEAYIESQKLQGWKAIPY
CCCCCCCCCCCHHEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCE
RYDDGGFSGGTMERPALQRLLADIDAGKIDVIVVYKIDRLSRSLLDFMKMIELFNEKEVS
EECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCE
FVSVTQHFSTTDSTGRMFLGILITFAQYEREVIGERIRDKVAAAKRRGKYCGGPAVLGYD
EEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEC
VDRENKKLLINQSEAPLVKLIFRRYTQVGSAKKVAQELNEQGYKTKSWTTKKGKERIGTE
CCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHCCCC
WNTAQVYRLLNNRLYIGEIAYKGKNYPAEHDALIDQNTWDKVQALLSENNRTKMSKARVK
CCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
MVSPLSGVIRCGHCDSAMGITYTNKGDRRYSYYICEKDTKRAVSRCPLKRVPAGDIESVV
HHCHHHHHEECCCCCCCCCEEEECCCCCEEEEEEECCHHHHHHHHCCCCCCCCCCHHHHH
LDQLGAVFRTPTLVAKTYFAAREIEAEERERLQVQKKELEQSLQSVRQEALKLMSPDNDD
HHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDRNSRLPLVNQQAVDLTKQLTNVSARLKVIDTEQISEGDVSEAFQSVKTFWEDLFPLER
CCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHH
NRLIQLLVETIEIRETGIDMELKTNGLTNLVTELAGLACEVRERSNS
HHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]