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The map label for this gene is phsB [H]

Identifier: 78355728

GI number: 78355728

Start: 697147

End: 698004

Strand: Reverse

Name: phsB [H]

Synonym: Dde_0681

Alternate gene names: 78355728

Gene position: 698004-697147 (Counterclockwise)

Preceding gene: 78355729

Following gene: 78355727

Centisome position: 18.71

GC content: 61.66

Gene sequence:

>858_bases
ATGACCCAGCAGTTCGCCATGGTTATCGATTCCGCCAGATGCATCGACTGCAAAGCGTGCGTGGTTTCCTGCAAAGTGGC
AAACAATGTTCCGGCAGGATTCTCACGCAACTGGATCAAAGCAGACGGACCGGTGGTCAAGGCGGGAAAAGGGCCTTTCG
GGCATTTCCAGCCGGGCGCGTGCATGCACTGCGAATCTCCCACCTGCGTGGAAGCCTGCCCCACAGGCGCCACATGGAAA
GACAGGGAGACCGGCATAGTGGAAATAGACCCCGCACTGTGCATCGGCTGCGGCAACTGCATTCCTGCATGCCCGTACGG
GGCGCGCTTCCGTAATCCGCAAAGGCGCATTGCCGACAAGTGCAACTACTGCCCCGAACGCCGTGCCGCCGGACTGCCCC
CCGCATGTGTGGACACATGCCCCACCAAGGCGCGGGTGTTCGGCGATATTCTGAACCCCGAAACAGAAGCCGCACGGCTG
CTTACCCGCAACAGCGGCCATGTGACGCAGCTGATACACGAAAAAAGCAACACGGCACCGCACATGTACTATGTCGGCGG
CACCGCACCCGCAGGCTGGATGCATGAAGCAAAGGCTCCGGCATCCATGCGCGCGCTTACCGGTGTTGTGGCACCGGGAG
TCAAGGGACTGGTGGCGCTTTCGGGCCTGGGCGTACTGGTTATGCTGGGCAGGCAGCTGCTGGACAGCAGAAAATCAGGC
GACGGAGGCCACGACCGGCCACACGCGTCCGGCAGTAATGCGTCCGGCAGCAGTGCTTCCGGCAGCAATGCTTCCGGCAG
CAATACATCCGGCACACAACACTCTGCGCCGGCAGACACCGAAGACAGCAGGGAGTAG

Upstream 100 bases:

>100_bases
TGGGAGCCAGCATAGCCGCCCTGCTGGAAGGGCACTATGACAGTGTTTCCGGCAATGCCGCCATGCACGAAACCTTTGTA
TCCGTCAGCGGGAGGGCATC

Downstream 100 bases:

>100_bases
GAGGACGCCATGAACAGAACATACAAGCGCCATGACCGCTCCGACATAGCCATACACTGGTTCAACGCTCTGTGCTGGCT
GCTGCTGCTTGTCAGCGGGC

Product: Fe-S-cluster-containing hydrogenase components 1-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTQQFAMVIDSARCIDCKACVVSCKVANNVPAGFSRNWIKADGPVVKAGKGPFGHFQPGACMHCESPTCVEACPTGATWK
DRETGIVEIDPALCIGCGNCIPACPYGARFRNPQRRIADKCNYCPERRAAGLPPACVDTCPTKARVFGDILNPETEAARL
LTRNSGHVTQLIHEKSNTAPHMYYVGGTAPAGWMHEAKAPASMRALTGVVAPGVKGLVALSGLGVLVMLGRQLLDSRKSG
DGGHDRPHASGSNASGSSASGSNASGSNTSGTQHSAPADTEDSRE

Sequences:

>Translated_285_residues
MTQQFAMVIDSARCIDCKACVVSCKVANNVPAGFSRNWIKADGPVVKAGKGPFGHFQPGACMHCESPTCVEACPTGATWK
DRETGIVEIDPALCIGCGNCIPACPYGARFRNPQRRIADKCNYCPERRAAGLPPACVDTCPTKARVFGDILNPETEAARL
LTRNSGHVTQLIHEKSNTAPHMYYVGGTAPAGWMHEAKAPASMRALTGVVAPGVKGLVALSGLGVLVMLGRQLLDSRKSG
DGGHDRPHASGSNASGSSASGSNASGSNTSGTQHSAPADTEDSRE
>Mature_284_residues
TQQFAMVIDSARCIDCKACVVSCKVANNVPAGFSRNWIKADGPVVKAGKGPFGHFQPGACMHCESPTCVEACPTGATWKD
RETGIVEIDPALCIGCGNCIPACPYGARFRNPQRRIADKCNYCPERRAAGLPPACVDTCPTKARVFGDILNPETEAARLL
TRNSGHVTQLIHEKSNTAPHMYYVGGTAPAGWMHEAKAPASMRALTGVVAPGVKGLVALSGLGVLVMLGRQLLDSRKSGD
GGHDRPHASGSNASGSSASGSNASGSNTSGTQHSAPADTEDSRE

Specific function: Electron transport protein for the PHS system oxidoreductase which produces hydrogen sulfide from thiosulfate (Potential) [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI226510944, Length=181, Percent_Identity=42.5414364640884, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI2367345, Length=183, Percent_Identity=39.8907103825137, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787122, Length=156, Percent_Identity=39.1025641025641, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787872, Length=156, Percent_Identity=38.4615384615385, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787749, Length=221, Percent_Identity=31.6742081447964, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790326, Length=215, Percent_Identity=32.5581395348837, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789370, Length=215, Percent_Identity=30.6976744186047, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=40.4040404040404, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787740, Length=117, Percent_Identity=35.042735042735, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082180, Length=158, Percent_Identity=33.5443037974684, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082179, Length=143, Percent_Identity=34.2657342657343, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367245, Length=149, Percent_Identity=33.5570469798658, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789067, Length=158, Percent_Identity=30.379746835443, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787963, Length=148, Percent_Identity=32.4324324324324, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788811, Length=171, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 29720; Mature: 29589

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQFAMVIDSARCIDCKACVVSCKVANNVPAGFSRNWIKADGPVVKAGKGPFGHFQPGA
CCCHHEEHHCCCCCEEHHHHEEEEHHCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCC
CMHCESPTCVEACPTGATWKDRETGIVEIDPALCIGCGNCIPACPYGARFRNPQRRIADK
EEECCCCCHHHHCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCHHCCCHHHHHHHH
CNYCPERRAAGLPPACVDTCPTKARVFGDILNPETEAARLLTRNSGHVTQLIHEKSNTAP
HCCCHHHHCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCC
HMYYVGGTAPAGWMHEAKAPASMRALTGVVAPGVKGLVALSGLGVLVMLGRQLLDSRKSG
EEEEECCCCCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGGHDRPHASGSNASGSSASGSNASGSNTSGTQHSAPADTEDSRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQQFAMVIDSARCIDCKACVVSCKVANNVPAGFSRNWIKADGPVVKAGKGPFGHFQPGA
CCHHEEHHCCCCCEEHHHHEEEEHHCCCCCCCCCCCEEECCCCEEECCCCCCCCCCCCC
CMHCESPTCVEACPTGATWKDRETGIVEIDPALCIGCGNCIPACPYGARFRNPQRRIADK
EEECCCCCHHHHCCCCCCCCCCCCCEEEECCCEEEECCCCCCCCCCCHHCCCHHHHHHHH
CNYCPERRAAGLPPACVDTCPTKARVFGDILNPETEAARLLTRNSGHVTQLIHEKSNTAP
HCCCHHHHCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHCCCCCC
HMYYVGGTAPAGWMHEAKAPASMRALTGVVAPGVKGLVALSGLGVLVMLGRQLLDSRKSG
EEEEECCCCCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
DGGHDRPHASGSNASGSSASGSNASGSNTSGTQHSAPADTEDSRE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]