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The map label for this gene is braC [H]

Identifier: 78355659

GI number: 78355659

Start: 635412

End: 636527

Strand: Reverse

Name: braC [H]

Synonym: Dde_0612

Alternate gene names: 78355659

Gene position: 636527-635412 (Counterclockwise)

Preceding gene: 78355660

Following gene: 78355658

Centisome position: 17.06

GC content: 57.8

Gene sequence:

>1116_bases
ATGGGTAAAGGATGGCTGAAATCTGTTGTTGCCGGTGTGGCACTGACCCTGCTGGCCGGTCCCGCTTTTGCCGACACCAT
CAAGCTGGGGGTGCCGGGGGCACACAGCGGCGACCTGGCTTCTTACGGGCTGCCCACCGTCAACGCAGCCAAGCTGGTTG
CGCAGCAGTACAACGAAAAAGGCGGCATACTGGGCATGCAGATAGAAGTGCTGCCGCAGGACGATCAGTGCAAACCCGAA
CTGGCCACCAACGCCGCCACCAAGCTTATTTCCGACAAAGTGACCGTTGTGCTGGGACACATCTGCTCCGGCGCCACCAA
GGCTGCGCTGCCCATCTACAAGGAAGCCAACATCGTTGTCATGTCGCCTTCCGCCACCAACCCGCCCCTCACCCAGAGCG
GCGAGTACCCGACATTTTTCCGCACCATTGCTTCCGACGACCAGCAGGCGAAACTGGGCGTTGATTTTGCCACGGAAAAG
CTGGGCCTGAAAAAAATCGCCGTGCTGCACGACAAGGGTGACTACGGCAAAGGCTATGCCGAATTTGCCAAGCTGTTCAT
CGAAAAAAGCGGCAAAGCCGAAGTGGTCATGTTTGAAGGCATCACTCCCGGCGCCATGGACTATTCCGCAGTGGTGCAGA
AACTGCGCCGTTCCGGTGCCGAAGGCGTGATGTTCGGCGGTTACCATCCCGAAGCATCCAAGCTGGTTTCCCAGATGCGC
AAAAAGCGCATGGACCTGCCGTTCATCTCTGACGACGGAGTAAAGGACGACACGTTTATCAAGGTTGCCGGTGAAAACGC
CGAGGGCGTTTACGCTACCAGCTCCAAAGACGTGAGCAAGCTGGCCATGTATCAGGAAGCCATTGCCAGACATAAGCAGG
AATTCGGCACCGAGCCGGGCGCCTTTTACAAGGAAGCTTACTCCGCCGCCATCGCCCTGCTGAACGCCGTGGAAAAAGCA
GGCAGCACCGACTCTGCCCTCATCATGGACGCGCTGCGCAACAACTTTGTGGAAACCCCGGTGGGCACCATCAAGTTCGA
CAGCATAGGCGATGCCGAAGGCGTCGGCTTCTCCATGTATCAGGTTCAGAACGGACAGTACGTGGAACTGAACTGA

Upstream 100 bases:

>100_bases
GACCGTCATCGGAAAGGATGCTGCGCTTCATATTCGCTTCAGCGGCACTTCTGAATAGTATTCACAAATTTGTTTTCTGG
ATTGAAACGGAGGAAGAATC

Downstream 100 bases:

>100_bases
CACGCAGACTGAGGCGGGGGGCAGGCGGATATCTGCCTGTCCCCCGCATTTTGCACACCATAACATCCCCACACGGCTGA
TTCCCGATGAAGAGCGCGGG

Product: high-affinity branched chain amino acid ABC transporter periplasmic branched chain amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MGKGWLKSVVAGVALTLLAGPAFADTIKLGVPGAHSGDLASYGLPTVNAAKLVAQQYNEKGGILGMQIEVLPQDDQCKPE
LATNAATKLISDKVTVVLGHICSGATKAALPIYKEANIVVMSPSATNPPLTQSGEYPTFFRTIASDDQQAKLGVDFATEK
LGLKKIAVLHDKGDYGKGYAEFAKLFIEKSGKAEVVMFEGITPGAMDYSAVVQKLRRSGAEGVMFGGYHPEASKLVSQMR
KKRMDLPFISDDGVKDDTFIKVAGENAEGVYATSSKDVSKLAMYQEAIARHKQEFGTEPGAFYKEAYSAAIALLNAVEKA
GSTDSALIMDALRNNFVETPVGTIKFDSIGDAEGVGFSMYQVQNGQYVELN

Sequences:

>Translated_371_residues
MGKGWLKSVVAGVALTLLAGPAFADTIKLGVPGAHSGDLASYGLPTVNAAKLVAQQYNEKGGILGMQIEVLPQDDQCKPE
LATNAATKLISDKVTVVLGHICSGATKAALPIYKEANIVVMSPSATNPPLTQSGEYPTFFRTIASDDQQAKLGVDFATEK
LGLKKIAVLHDKGDYGKGYAEFAKLFIEKSGKAEVVMFEGITPGAMDYSAVVQKLRRSGAEGVMFGGYHPEASKLVSQMR
KKRMDLPFISDDGVKDDTFIKVAGENAEGVYATSSKDVSKLAMYQEAIARHKQEFGTEPGAFYKEAYSAAIALLNAVEKA
GSTDSALIMDALRNNFVETPVGTIKFDSIGDAEGVGFSMYQVQNGQYVELN
>Mature_370_residues
GKGWLKSVVAGVALTLLAGPAFADTIKLGVPGAHSGDLASYGLPTVNAAKLVAQQYNEKGGILGMQIEVLPQDDQCKPEL
ATNAATKLISDKVTVVLGHICSGATKAALPIYKEANIVVMSPSATNPPLTQSGEYPTFFRTIASDDQQAKLGVDFATEKL
GLKKIAVLHDKGDYGKGYAEFAKLFIEKSGKAEVVMFEGITPGAMDYSAVVQKLRRSGAEGVMFGGYHPEASKLVSQMRK
KRMDLPFISDDGVKDDTFIKVAGENAEGVYATSSKDVSKLAMYQEAIARHKQEFGTEPGAFYKEAYSAAIALLNAVEKAG
STDSALIMDALRNNFVETPVGTIKFDSIGDAEGVGFSMYQVQNGQYVELN

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=355, Percent_Identity=32.9577464788732, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI48994941, Length=354, Percent_Identity=33.3333333333333, Blast_Score=202, Evalue=4e-53,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39515; Mature: 39384

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKGWLKSVVAGVALTLLAGPAFADTIKLGVPGAHSGDLASYGLPTVNAAKLVAQQYNEK
CCHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHCCC
GGILGMQIEVLPQDDQCKPELATNAATKLISDKVTVVLGHICSGATKAALPIYKEANIVV
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCEEE
MSPSATNPPLTQSGEYPTFFRTIASDDQQAKLGVDFATEKLGLKKIAVLHDKGDYGKGYA
ECCCCCCCCCCCCCCCCHHHHHHCCCCCCCEECCCHHHHHHCCEEEEEEECCCCCCCCHH
EFAKLFIEKSGKAEVVMFEGITPGAMDYSAVVQKLRRSGAEGVMFGGYHPEASKLVSQMR
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
KKRMDLPFISDDGVKDDTFIKVAGENAEGVYATSSKDVSKLAMYQEAIARHKQEFGTEPG
HHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
AFYKEAYSAAIALLNAVEKAGSTDSALIMDALRNNFVETPVGTIKFDSIGDAEGVGFSMY
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEE
QVQNGQYVELN
EECCCEEEEEC
>Mature Secondary Structure 
GKGWLKSVVAGVALTLLAGPAFADTIKLGVPGAHSGDLASYGLPTVNAAKLVAQQYNEK
CHHHHHHHHHHHHHHHHCCCCHHHEEEECCCCCCCCCCHHCCCCCCHHHHHHHHHHCCC
GGILGMQIEVLPQDDQCKPELATNAATKLISDKVTVVLGHICSGATKAALPIYKEANIVV
CCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEECCCEEE
MSPSATNPPLTQSGEYPTFFRTIASDDQQAKLGVDFATEKLGLKKIAVLHDKGDYGKGYA
ECCCCCCCCCCCCCCCCHHHHHHCCCCCCCEECCCHHHHHHCCEEEEEEECCCCCCCCHH
EFAKLFIEKSGKAEVVMFEGITPGAMDYSAVVQKLRRSGAEGVMFGGYHPEASKLVSQMR
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
KKRMDLPFISDDGVKDDTFIKVAGENAEGVYATSSKDVSKLAMYQEAIARHKQEFGTEPG
HHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCC
AFYKEAYSAAIALLNAVEKAGSTDSALIMDALRNNFVETPVGTIKFDSIGDAEGVGFSMY
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCEEEE
QVQNGQYVELN
EECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]