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The map label for this gene is dosC [H]

Identifier: 78355616

GI number: 78355616

Start: 593649

End: 594860

Strand: Reverse

Name: dosC [H]

Synonym: Dde_0569

Alternate gene names: 78355616

Gene position: 594860-593649 (Counterclockwise)

Preceding gene: 78355622

Following gene: 78355614

Centisome position: 15.95

GC content: 54.46

Gene sequence:

>1212_bases
GTGCTAACCGGTCTGCGCTCAATTCAACTTATCGGAATTTACCCTTGCGGGCGCCTCTATTTCACACGCCCGGCATATAC
GGAATGCGTCTTGAAGCAACATCTGCGTATAAAACTGCATCAGACACTGCACAGCGGAACAGAGAAAGACAGTGATCCTG
AATTAGTCCGCCGCACTTATATGCTGAACGGCTTTCTGCTGCTCAGTGCCTTTATATGTGCACTGATGGCAGTACTGAAA
CATACCGAAAGCTTTACGGTGCTTAAAGGCATTTTATGGTGTTCTGCAGGGGCATACTGTGTGTTATACTTTTTTTTGCG
CACAAGCGGTATGCTTAAAGTGGCAAGCGACTTTGCGCTGGCTCTTACCGTCACACTCGGCGTACTGCTTATCTGGCGTC
TGAATGACCAGATAGCATATATCCTCTGGCTTTTCGCACTGCCGCCTGCAGCGTTTTTCACCTTCGGTACAAAGCGTGCA
GCTGTGGCGCTGGGGCTCCTCCTTATTGCGCTGCTGCTCAGCCCTTTTGTTTCTCCCGCAGCCATGCCGCCGGAAACGTT
TTCGCTGTCTTACTTTGTGCGCGTAATTGCCGCTTTCTTTACTCTGAGCCTGTTTTCCGCACTGGGTGAATATTCACGCG
CACACACGCAACGGACACTGGTGGCCCTTACCAGAGAAATGGAACAATGCGCGTGCACCGACCCGCTGACAGGTCTGCTC
AACCGGCGGGGCATATACGACAGACTTAACGAAGAACGGGCGCGGGCCACACGGTCGGGTTCCACATTTTCGGTGATACT
GTGCGACGTGGACCACTTCAAGAACGTCAACGACTGTTTCGGCCATCAGTGCGGCGACCATGTGCTGCACCAGCTTGCCG
AGCACTTCCGCCGCAGCGTGCGCGGGCAGGATGTCGTCTGCCGCTGGGGAGGCGAGGAATTCCTCTTCATTCTGCCGGAA
ACCGACGAGCAGGGAGCCTTGAATCTGGCGGAGAAACTACGCTCCACCGTGGAACAGACGCCCGTCAATTACCACGGCAT
GCCCATAGACATCACACTGAGTCTGGGCGTGCAGGAATGCACCCTGACGGAAAGCACCGAATTCCACATCCGCGTCGCGG
ACCAGAAGCTTTACATAGCCAAGGAACACGGCCGCAACAAAGTGGTCAGCGGCAACATAGAGGAACTGATGCCGCAGGAA
CTCATAGCCTGA

Upstream 100 bases:

>100_bases
TTATAACGCCTTGGCTCCTGCAGCATACACCATTTAATCCGCAGGCCCTACACTTTTTTGCTTATCGGAGCCAACCCCCT
ATAGCCACCACGCATTTTCT

Downstream 100 bases:

>100_bases
CGCCGTTTTGTTCTTCTGCCGCCATGCATCCGGCGGACACTGTGCACATAAAGAAAGGGCGGCCCGCGGGCCGCCCTTAT
GCATGCATCATATGCACCGG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLTGLRSIQLIGIYPCGRLYFTRPAYTECVLKQHLRIKLHQTLHSGTEKDSDPELVRRTYMLNGFLLLSAFICALMAVLK
HTESFTVLKGILWCSAGAYCVLYFFLRTSGMLKVASDFALALTVTLGVLLIWRLNDQIAYILWLFALPPAAFFTFGTKRA
AVALGLLLIALLLSPFVSPAAMPPETFSLSYFVRVIAAFFTLSLFSALGEYSRAHTQRTLVALTREMEQCACTDPLTGLL
NRRGIYDRLNEERARATRSGSTFSVILCDVDHFKNVNDCFGHQCGDHVLHQLAEHFRRSVRGQDVVCRWGGEEFLFILPE
TDEQGALNLAEKLRSTVEQTPVNYHGMPIDITLSLGVQECTLTESTEFHIRVADQKLYIAKEHGRNKVVSGNIEELMPQE
LIA

Sequences:

>Translated_403_residues
MLTGLRSIQLIGIYPCGRLYFTRPAYTECVLKQHLRIKLHQTLHSGTEKDSDPELVRRTYMLNGFLLLSAFICALMAVLK
HTESFTVLKGILWCSAGAYCVLYFFLRTSGMLKVASDFALALTVTLGVLLIWRLNDQIAYILWLFALPPAAFFTFGTKRA
AVALGLLLIALLLSPFVSPAAMPPETFSLSYFVRVIAAFFTLSLFSALGEYSRAHTQRTLVALTREMEQCACTDPLTGLL
NRRGIYDRLNEERARATRSGSTFSVILCDVDHFKNVNDCFGHQCGDHVLHQLAEHFRRSVRGQDVVCRWGGEEFLFILPE
TDEQGALNLAEKLRSTVEQTPVNYHGMPIDITLSLGVQECTLTESTEFHIRVADQKLYIAKEHGRNKVVSGNIEELMPQE
LIA
>Mature_403_residues
MLTGLRSIQLIGIYPCGRLYFTRPAYTECVLKQHLRIKLHQTLHSGTEKDSDPELVRRTYMLNGFLLLSAFICALMAVLK
HTESFTVLKGILWCSAGAYCVLYFFLRTSGMLKVASDFALALTVTLGVLLIWRLNDQIAYILWLFALPPAAFFTFGTKRA
AVALGLLLIALLLSPFVSPAAMPPETFSLSYFVRVIAAFFTLSLFSALGEYSRAHTQRTLVALTREMEQCACTDPLTGLL
NRRGIYDRLNEERARATRSGSTFSVILCDVDHFKNVNDCFGHQCGDHVLHQLAEHFRRSVRGQDVVCRWGGEEFLFILPE
TDEQGALNLAEKLRSTVEQTPVNYHGMPIDITLSLGVQECTLTESTEFHIRVADQKLYIAKEHGRNKVVSGNIEELMPQE
LIA

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=180, Percent_Identity=39.4444444444444, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787262, Length=174, Percent_Identity=36.7816091954023, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=38.4615384615385, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787802, Length=177, Percent_Identity=34.4632768361582, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082007, Length=177, Percent_Identity=36.7231638418079, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=29.8342541436464, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081974, Length=166, Percent_Identity=28.3132530120482, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=30.9090909090909, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=156, Percent_Identity=32.6923076923077, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788381, Length=170, Percent_Identity=28.8235294117647, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=31.2101910828025, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081921, Length=180, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788956, Length=201, Percent_Identity=30.3482587064677, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787541, Length=159, Percent_Identity=29.559748427673, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 45264; Mature: 45264

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGLRSIQLIGIYPCGRLYFTRPAYTECVLKQHLRIKLHQTLHSGTEKDSDPELVRRTY
CCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MLNGFLLLSAFICALMAVLKHTESFTVLKGILWCSAGAYCVLYFFLRTSGMLKVASDFAL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVTLGVLLIWRLNDQIAYILWLFALPPAAFFTFGTKRAAVALGLLLIALLLSPFVSPA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AMPPETFSLSYFVRVIAAFFTLSLFSALGEYSRAHTQRTLVALTREMEQCACTDPLTGLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NRRGIYDRLNEERARATRSGSTFSVILCDVDHFKNVNDCFGHQCGDHVLHQLAEHFRRSV
HCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHCCCCHHHHCCCHHHHHHHHHHHHHHHHC
RGQDVVCRWGGEEFLFILPETDEQGALNLAEKLRSTVEQTPVNYHGMPIDITLSLGVQEC
CCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEE
TLTESTEFHIRVADQKLYIAKEHGRNKVVSGNIEELMPQELIA
EECCCCEEEEEEECCEEEEEHHCCCCCCCCCCHHHHCHHHHCC
>Mature Secondary Structure
MLTGLRSIQLIGIYPCGRLYFTRPAYTECVLKQHLRIKLHQTLHSGTEKDSDPELVRRTY
CCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
MLNGFLLLSAFICALMAVLKHTESFTVLKGILWCSAGAYCVLYFFLRTSGMLKVASDFAL
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVTLGVLLIWRLNDQIAYILWLFALPPAAFFTFGTKRAAVALGLLLIALLLSPFVSPA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
AMPPETFSLSYFVRVIAAFFTLSLFSALGEYSRAHTQRTLVALTREMEQCACTDPLTGLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
NRRGIYDRLNEERARATRSGSTFSVILCDVDHFKNVNDCFGHQCGDHVLHQLAEHFRRSV
HCCCHHHHHHHHHHHHHCCCCEEEEEEEECHHCCCCHHHHCCCHHHHHHHHHHHHHHHHC
RGQDVVCRWGGEEFLFILPETDEQGALNLAEKLRSTVEQTPVNYHGMPIDITLSLGVQEC
CCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEE
TLTESTEFHIRVADQKLYIAKEHGRNKVVSGNIEELMPQELIA
EECCCCEEEEEEECCEEEEEHHCCCCCCCCCCHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]