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The map label for this gene is rne [H]

Identifier: 78355584

GI number: 78355584

Start: 547672

End: 549132

Strand: Reverse

Name: rne [H]

Synonym: Dde_0537

Alternate gene names: 78355584

Gene position: 549132-547672 (Counterclockwise)

Preceding gene: 78355585

Following gene: 304570596

Centisome position: 14.72

GC content: 56.4

Gene sequence:

>1461_bases
ATGGAAAAGACCCGCAAGGGCAAACGCAAGATGTTCATCAGCGTGCTGCCCGAAGAACAGGTTGAAGTTGTTCTCTCTGA
AGAAGGCGTAATTGTTGAATATTATGTAGAGATGGTGCATCAGGCCAAGACCAAAGGCAACATCTACAAAGGCATCATCA
ACAACATAGACTCCAACCTGCAGGCCGCCTTTGTCAATTACGGCGCCGAAAAAAACGGTTTTCTGCAGGTGGATGAAGTT
CACCCCGAATACTACTCGGCCCCCCACGACCCTTCCAAAGGCAAAAAATATCCTCTGCTGCAGAAAGTGCTTAAAGCAGG
ACAGGAAGTACTGGTGCAGGTGGTCAAGGAACCCACGGGTTCCAAAGGTGCCTTCCTCACCTCGTATCTTTCGCTGCCGG
GACGTTTTCTGGTGCTCACCCCCGGACGCGAACAGATAGGCGTTTCGCGCAAGGTGGAATCGGAAGAAGAAAGAGTGCGC
CTGCGCGCCCTGCTTGAAGGTGCCACCCCCGGTCCCGGGCTGGGGCTTATCGTACGCACGGTTTCGGTGGGGCAGAGCAA
AACAAGCATACAGCGCGACCTGCAGTTTCTTAAACGCCTGTGGAAGGATGTGCGCAAAAAAGGCACCGTGGAAAAAGCCC
CCAGCCTTATCTACAAGGAACTGGACCTTGCCGCACGGGCAGTGCGCGACTATCTGACCGACGATGTGGGCGAAGTGTGG
GTGGACGACGAACAGACAGCCGATGCCGTGCAGGAATTTGCCGCCATCGTCTTTCCGCGGCGCACCAATCTGGTCAAACG
GCATAAAGACACCGACCGCACACTCTGGGAGCGGTTCAGCATGCAGAAGCAGCTGGACCAGATATATTCGCGCGAAGTAT
CGCTGCCCAGCGGCGGCAGGCTGGTTTTCGACCAGACCGAAGCGCTTATGGCCATCGATATCAACTCCGGAAAAATCGCC
GGCAAGACAAATTTCGAATCCATGGCTCTGCGCACCAATATGGAAGCCGCCGAAACCATCGCCCAGCAGCTCAAGCTGCG
TGACATCGGCGGGCAGATAGTTGTCGACTTCATTGAAATGCGCGACAGAAACCACTGGCGCGAAGTGGAAAAAACCCTGC
GCGCCGCCATGAAAAACGACCGCGCCAGACACGATGTGGGCAAAATGAGCCGTTTCGGCCTGCTGCAGATAGTGCGGCAG
CGTCTGGGGTCTTCCGCCCTGTCCATAAGCACCGAACCCTGCCCGTGCTGCAAAGGCACAGGGCTGCGCCGCAACATGGA
ATGGCAGGCCATGCAGGCCCTGAAGGATATCCACAGACAGCTGCGCGCCACCACGGCCTCTTCGCTGCGCTGGGAAGCTC
CGGAAGAACTGGGCATGTACCTGCTGAACCATAAACGCGGACACCTGACCGAACTGGAAAACAGGTTCGAACGCGACATC
GACATCGCCATACGGAGGTAG

Upstream 100 bases:

>100_bases
GGACGAAGACCCGTTCTTCGCCCTGGCAGACAGATAACCGGCACATCTGTTCCGTCGCACGGGATTACCCGTACACTTTT
TTTATCCTCATGAGGTTCTC

Downstream 100 bases:

>100_bases
GCATGGCAAGAGTGCTTGTGCACGCATGCTGCGGGCCTTGCTCCATCACCACCATGCAGACGCTGCTGCGCGAAGGACAC
GAAGTCACCGGCCTGTTCTT

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MEKTRKGKRKMFISVLPEEQVEVVLSEEGVIVEYYVEMVHQAKTKGNIYKGIINNIDSNLQAAFVNYGAEKNGFLQVDEV
HPEYYSAPHDPSKGKKYPLLQKVLKAGQEVLVQVVKEPTGSKGAFLTSYLSLPGRFLVLTPGREQIGVSRKVESEEERVR
LRALLEGATPGPGLGLIVRTVSVGQSKTSIQRDLQFLKRLWKDVRKKGTVEKAPSLIYKELDLAARAVRDYLTDDVGEVW
VDDEQTADAVQEFAAIVFPRRTNLVKRHKDTDRTLWERFSMQKQLDQIYSREVSLPSGGRLVFDQTEALMAIDINSGKIA
GKTNFESMALRTNMEAAETIAQQLKLRDIGGQIVVDFIEMRDRNHWREVEKTLRAAMKNDRARHDVGKMSRFGLLQIVRQ
RLGSSALSISTEPCPCCKGTGLRRNMEWQAMQALKDIHRQLRATTASSLRWEAPEELGMYLLNHKRGHLTELENRFERDI
DIAIRR

Sequences:

>Translated_486_residues
MEKTRKGKRKMFISVLPEEQVEVVLSEEGVIVEYYVEMVHQAKTKGNIYKGIINNIDSNLQAAFVNYGAEKNGFLQVDEV
HPEYYSAPHDPSKGKKYPLLQKVLKAGQEVLVQVVKEPTGSKGAFLTSYLSLPGRFLVLTPGREQIGVSRKVESEEERVR
LRALLEGATPGPGLGLIVRTVSVGQSKTSIQRDLQFLKRLWKDVRKKGTVEKAPSLIYKELDLAARAVRDYLTDDVGEVW
VDDEQTADAVQEFAAIVFPRRTNLVKRHKDTDRTLWERFSMQKQLDQIYSREVSLPSGGRLVFDQTEALMAIDINSGKIA
GKTNFESMALRTNMEAAETIAQQLKLRDIGGQIVVDFIEMRDRNHWREVEKTLRAAMKNDRARHDVGKMSRFGLLQIVRQ
RLGSSALSISTEPCPCCKGTGLRRNMEWQAMQALKDIHRQLRATTASSLRWEAPEELGMYLLNHKRGHLTELENRFERDI
DIAIRR
>Mature_486_residues
MEKTRKGKRKMFISVLPEEQVEVVLSEEGVIVEYYVEMVHQAKTKGNIYKGIINNIDSNLQAAFVNYGAEKNGFLQVDEV
HPEYYSAPHDPSKGKKYPLLQKVLKAGQEVLVQVVKEPTGSKGAFLTSYLSLPGRFLVLTPGREQIGVSRKVESEEERVR
LRALLEGATPGPGLGLIVRTVSVGQSKTSIQRDLQFLKRLWKDVRKKGTVEKAPSLIYKELDLAARAVRDYLTDDVGEVW
VDDEQTADAVQEFAAIVFPRRTNLVKRHKDTDRTLWERFSMQKQLDQIYSREVSLPSGGRLVFDQTEALMAIDINSGKIA
GKTNFESMALRTNMEAAETIAQQLKLRDIGGQIVVDFIEMRDRNHWREVEKTLRAAMKNDRARHDVGKMSRFGLLQIVRQ
RLGSSALSISTEPCPCCKGTGLRRNMEWQAMQALKDIHRQLRATTASSLRWEAPEELGMYLLNHKRGHLTELENRFERDI
DIAIRR

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=469, Percent_Identity=39.4456289978678, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI87082235, Length=430, Percent_Identity=33.0232558139535, Blast_Score=249, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 55293; Mature: 55293

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTRKGKRKMFISVLPEEQVEVVLSEEGVIVEYYVEMVHQAKTKGNIYKGIINNIDSNL
CCCCCCCCHHEEEEECCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
QAAFVNYGAEKNGFLQVDEVHPEYYSAPHDPSKGKKYPLLQKVLKAGQEVLVQVVKEPTG
HHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SKGAFLTSYLSLPGRFLVLTPGREQIGVSRKVESEEERVRLRALLEGATPGPGLGLIVRT
CCCHHHHHHHCCCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHEEE
VSVGQSKTSIQRDLQFLKRLWKDVRKKGTVEKAPSLIYKELDLAARAVRDYLTDDVGEVW
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCEE
VDDEQTADAVQEFAAIVFPRRTNLVKRHKDTDRTLWERFSMQKQLDQIYSREVSLPSGGR
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LVFDQTEALMAIDINSGKIAGKTNFESMALRTNMEAAETIAQQLKLRDIGGQIVVDFIEM
EEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
RDRNHWREVEKTLRAAMKNDRARHDVGKMSRFGLLQIVRQRLGSSALSISTEPCPCCKGT
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
GLRRNMEWQAMQALKDIHRQLRATTASSLRWEAPEELGMYLLNHKRGHLTELENRFERDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCH
DIAIRR
HHEECC
>Mature Secondary Structure
MEKTRKGKRKMFISVLPEEQVEVVLSEEGVIVEYYVEMVHQAKTKGNIYKGIINNIDSNL
CCCCCCCCHHEEEEECCCHHHEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
QAAFVNYGAEKNGFLQVDEVHPEYYSAPHDPSKGKKYPLLQKVLKAGQEVLVQVVKEPTG
HHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
SKGAFLTSYLSLPGRFLVLTPGREQIGVSRKVESEEERVRLRALLEGATPGPGLGLIVRT
CCCHHHHHHHCCCCCEEEECCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHEEE
VSVGQSKTSIQRDLQFLKRLWKDVRKKGTVEKAPSLIYKELDLAARAVRDYLTDDVGEVW
HHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCEE
VDDEQTADAVQEFAAIVFPRRTNLVKRHKDTDRTLWERFSMQKQLDQIYSREVSLPSGGR
CCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
LVFDQTEALMAIDINSGKIAGKTNFESMALRTNMEAAETIAQQLKLRDIGGQIVVDFIEM
EEEECCCEEEEEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
RDRNHWREVEKTLRAAMKNDRARHDVGKMSRFGLLQIVRQRLGSSALSISTEPCPCCKGT
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCC
GLRRNMEWQAMQALKDIHRQLRATTASSLRWEAPEELGMYLLNHKRGHLTELENRFERDI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCH
DIAIRR
HHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]