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The map label for this gene is fliF [H]

Identifier: 78355400

GI number: 78355400

Start: 342787

End: 344406

Strand: Reverse

Name: fliF [H]

Synonym: Dde_0353

Alternate gene names: 78355400

Gene position: 344406-342787 (Counterclockwise)

Preceding gene: 78355401

Following gene: 78355399

Centisome position: 9.23

GC content: 57.16

Gene sequence:

>1620_bases
ATGACCGCGCTCAATTCCGCCGTGGATAAGGCAAAAACTTTCTGGACCCGCATCAGCATGTCGCAGCGGGTATTCATCGT
CGGCCTGACTGTGGTCGTGGTGGGCATATTTTTCGGCCTTATCTTCTGGATAAATCAGCCCGACTACAGGGTGCTGTATT
CCAAGCTTACCCCGGAAGACGCCAACAGGGTGGTCACCGTGCTGCAGGCGCAGAAGGTCAGGTACCGGCTGGATAACGGC
GGCAGCACCATAATGGTACCCGCCGACCGCGTGTACGACATGCGGCTTATGGTTGCCGGTGAAGGTTCAGTGGTGGGACA
GGGTGTCGGCTTTGAAATTTTTGACCAGATCAAGGTCGGTCAGACTGATTTTGTTCAGAAAATCAATTACCAGCGCGCCC
TGCAGGGCGAGCTTTCGCGTACCATAGCTGAATTTCCCGCTGTGGAGAGCGCCCGCGTGCATCTGGTCATTCCGCACCGC
AGCCTGTTCATCGAAGAGCAGATGCAGCCTTCCGCTTCTGTGGTGCTGAAGCTGCAGGACGGCAAAAAGATGGACGAAAA
GGACGTGCAGGCCGTCGTCAATCTGGTCACCATGTCTGTGGAAGGGCTGGACCAGGGCAAAATTTCCGTGGCGGACACTT
CGGGCAAGATTCTGTACTACCCCAAAGACGAACAGTCGCTTGACGGCATGACCTCCACCCAGCTGGAACACAAGCTGACC
ATGCAGCAGAATTTCGAACGCCGGATCGAAGAGCTGCTCTACCCTGTCATCGGCCCCGGCAAAGTCATTGCCAAGGTCAA
TGCCGATCTTGATTTTTCGCAGAAAACCATCCGCAAAAAGCTGTTTGACCCCGAAAGCTCTGTGGTACGCAGCGAAATAC
GTACCGAAGAGGCCCGCAAAGGCACGTCAAACGTCAATGCCGGCGTGCCGGAAGCGAACTTCCGCGGCGACGGCATGTCC
GGCGGCACCAGCACGGAAGACTCCAACCGTGAAACCCGCAACACCAACTACGAAATAAACGAAGAAGAACAGAATATCAT
CGCTCCCGTGGGGCAGCTTGACCGGCTGAGTGTTGCGGTTATCGTCGACGGCACGTATGAAAAGAACGAAGAAACGGGCG
AATGGACATATGTGCCGCGCAGCGACGAAGAGGTGGCGCGCATCCGCCAGCTGGTGGCCAGTGCCGTCGGGTTCGACCGT
GCCCGCGGCGATGTGCTGGAAGTCAGCAGCATATCCTTCGGCGGGCCGGAAGCCGACACCTACATGTCGCTGGCCGAAGT
CATCATGGACTACGCGCTGCGACTGGGTAAACCCCTGCTGAACGCGCTGCTGGTCTTCCTCTTCCTCGTCATGGTGGTGC
GGCCCGTGGTTCTGGCCCTCATACGGCCCAAGGTCGAAGGTGAAGTGGTCGAAGGCCTGGAAGGTCTGCCCACCGGCGAA
GAGCGTCTGGCCCTTATCGAGGGCGAAGAAGACATGGACGCCATGGACGCCCTGAAAAAAATCGACGATATCAAAGCCCA
TGCGCTGCAGCTTTCCGAACAGAACATGGAACAGGCAGTGGGTATTCTGAAAAGCTGGCTGAAGGACAGCGAAGGAGCCA
AAGTTGGCGGAGCAGCTTAA

Upstream 100 bases:

>100_bases
TCCGCAGTACGCAACAAAGTGATGGAAGCGTACCGCGAACTGAGCAAAATGCAGTTCTAAAACCGGCTGATTCCCGCTAC
TTATTTTTCCGGAGAAACAC

Downstream 100 bases:

>100_bases
GGGTCCCCGTAAGACCGCGATTCTCCTCCTTGCCATGGGGGACAAGTTCACCTCTGAAGTATTCAAAAGGCTGGACCGGT
CTGAGATCACGGCCATATCC

Product: flagellar M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTALNSAVDKAKTFWTRISMSQRVFIVGLTVVVVGIFFGLIFWINQPDYRVLYSKLTPEDANRVVTVLQAQKVRYRLDNG
GSTIMVPADRVYDMRLMVAGEGSVVGQGVGFEIFDQIKVGQTDFVQKINYQRALQGELSRTIAEFPAVESARVHLVIPHR
SLFIEEQMQPSASVVLKLQDGKKMDEKDVQAVVNLVTMSVEGLDQGKISVADTSGKILYYPKDEQSLDGMTSTQLEHKLT
MQQNFERRIEELLYPVIGPGKVIAKVNADLDFSQKTIRKKLFDPESSVVRSEIRTEEARKGTSNVNAGVPEANFRGDGMS
GGTSTEDSNRETRNTNYEINEEEQNIIAPVGQLDRLSVAVIVDGTYEKNEETGEWTYVPRSDEEVARIRQLVASAVGFDR
ARGDVLEVSSISFGGPEADTYMSLAEVIMDYALRLGKPLLNALLVFLFLVMVVRPVVLALIRPKVEGEVVEGLEGLPTGE
ERLALIEGEEDMDAMDALKKIDDIKAHALQLSEQNMEQAVGILKSWLKDSEGAKVGGAA

Sequences:

>Translated_539_residues
MTALNSAVDKAKTFWTRISMSQRVFIVGLTVVVVGIFFGLIFWINQPDYRVLYSKLTPEDANRVVTVLQAQKVRYRLDNG
GSTIMVPADRVYDMRLMVAGEGSVVGQGVGFEIFDQIKVGQTDFVQKINYQRALQGELSRTIAEFPAVESARVHLVIPHR
SLFIEEQMQPSASVVLKLQDGKKMDEKDVQAVVNLVTMSVEGLDQGKISVADTSGKILYYPKDEQSLDGMTSTQLEHKLT
MQQNFERRIEELLYPVIGPGKVIAKVNADLDFSQKTIRKKLFDPESSVVRSEIRTEEARKGTSNVNAGVPEANFRGDGMS
GGTSTEDSNRETRNTNYEINEEEQNIIAPVGQLDRLSVAVIVDGTYEKNEETGEWTYVPRSDEEVARIRQLVASAVGFDR
ARGDVLEVSSISFGGPEADTYMSLAEVIMDYALRLGKPLLNALLVFLFLVMVVRPVVLALIRPKVEGEVVEGLEGLPTGE
ERLALIEGEEDMDAMDALKKIDDIKAHALQLSEQNMEQAVGILKSWLKDSEGAKVGGAA
>Mature_538_residues
TALNSAVDKAKTFWTRISMSQRVFIVGLTVVVVGIFFGLIFWINQPDYRVLYSKLTPEDANRVVTVLQAQKVRYRLDNGG
STIMVPADRVYDMRLMVAGEGSVVGQGVGFEIFDQIKVGQTDFVQKINYQRALQGELSRTIAEFPAVESARVHLVIPHRS
LFIEEQMQPSASVVLKLQDGKKMDEKDVQAVVNLVTMSVEGLDQGKISVADTSGKILYYPKDEQSLDGMTSTQLEHKLTM
QQNFERRIEELLYPVIGPGKVIAKVNADLDFSQKTIRKKLFDPESSVVRSEIRTEEARKGTSNVNAGVPEANFRGDGMSG
GTSTEDSNRETRNTNYEINEEEQNIIAPVGQLDRLSVAVIVDGTYEKNEETGEWTYVPRSDEEVARIRQLVASAVGFDRA
RGDVLEVSSISFGGPEADTYMSLAEVIMDYALRLGKPLLNALLVFLFLVMVVRPVVLALIRPKVEGEVVEGLEGLPTGEE
RLALIEGEEDMDAMDALKKIDDIKAHALQLSEQNMEQAVGILKSWLKDSEGAKVGGAA

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=436, Percent_Identity=33.0275229357798, Blast_Score=235, Evalue=6e-63,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59631; Mature: 59500

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALNSAVDKAKTFWTRISMSQRVFIVGLTVVVVGIFFGLIFWINQPDYRVLYSKLTPED
CCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCCH
ANRVVTVLQAQKVRYRLDNGGSTIMVPADRVYDMRLMVAGEGSVVGQGVGFEIFDQIKVG
HHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEEEEEEECCCCEEECCCCHHHHHHHCCC
QTDFVQKINYQRALQGELSRTIAEFPAVESARVHLVIPHRSLFIEEQMQPSASVVLKLQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECC
GKKMDEKDVQAVVNLVTMSVEGLDQGKISVADTSGKILYYPKDEQSLDGMTSTQLEHKLT
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
MQQNFERRIEELLYPVIGPGKVIAKVNADLDFSQKTIRKKLFDPESSVVRSEIRTEEARK
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GTSNVNAGVPEANFRGDGMSGGTSTEDSNRETRNTNYEINEEEQNIIAPVGQLDRLSVAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCCCCCEEEEE
IVDGTYEKNEETGEWTYVPRSDEEVARIRQLVASAVGFDRARGDVLEVSSISFGGPEADT
EEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEEECCCCCCCHHH
YMSLAEVIMDYALRLGKPLLNALLVFLFLVMVVRPVVLALIRPKVEGEVVEGLEGLPTGE
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCC
ERLALIEGEEDMDAMDALKKIDDIKAHALQLSEQNMEQAVGILKSWLKDSEGAKVGGAA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TALNSAVDKAKTFWTRISMSQRVFIVGLTVVVVGIFFGLIFWINQPDYRVLYSKLTPED
CCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCCH
ANRVVTVLQAQKVRYRLDNGGSTIMVPADRVYDMRLMVAGEGSVVGQGVGFEIFDQIKVG
HHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEEEEEEECCCCEEECCCCHHHHHHHCCC
QTDFVQKINYQRALQGELSRTIAEFPAVESARVHLVIPHRSLFIEEQMQPSASVVLKLQD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHCCCCEEEEEEECC
GKKMDEKDVQAVVNLVTMSVEGLDQGKISVADTSGKILYYPKDEQSLDGMTSTQLEHKLT
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHH
MQQNFERRIEELLYPVIGPGKVIAKVNADLDFSQKTIRKKLFDPESSVVRSEIRTEEARK
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GTSNVNAGVPEANFRGDGMSGGTSTEDSNRETRNTNYEINEEEQNIIAPVGQLDRLSVAV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCCCCCEEEEE
IVDGTYEKNEETGEWTYVPRSDEEVARIRQLVASAVGFDRARGDVLEVSSISFGGPEADT
EEECCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCHHCCCCEEEEEECCCCCCCHHH
YMSLAEVIMDYALRLGKPLLNALLVFLFLVMVVRPVVLALIRPKVEGEVVEGLEGLPTGE
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCC
ERLALIEGEEDMDAMDALKKIDDIKAHALQLSEQNMEQAVGILKSWLKDSEGAKVGGAA
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002583; 11259647 [H]