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The map label for this gene is artJ [H]

Identifier: 78355233

GI number: 78355233

Start: 171627

End: 172382

Strand: Reverse

Name: artJ [H]

Synonym: Dde_0186

Alternate gene names: 78355233

Gene position: 172382-171627 (Counterclockwise)

Preceding gene: 78355235

Following gene: 78355232

Centisome position: 4.62

GC content: 56.35

Gene sequence:

>756_bases
ATGTTGAGGAAGTTCACCATCATGGCAGCAGTGCTGGCGCTTTCGCTGGCCTTCGCAGGAACCGCTTTTGCGGGCAAGAC
CCTCATCAACGGCATTGACGCCAACTACCCGCCCTTTGCCTATGTGGACAAAGACGGCAAACCCGCAGGCTTCGACGTGG
ACGCCATGGAATGGATTGCCGCAAAAATGGGATTCACCGTAAAGCACAAGCCCATGGAATGGTCCACCATCGTACAGAGC
CTTGTCTCCAAAAAAATCGACATGGTCTGTTCCGGCATGAGCATCACCGAAGAGCGCGCCCGTCAGGTCAGCTTCTCCGA
CCCGTACTGGACCGTGGCGCAGGTATTCATCGCCAAAAAAGGCTCCGGCCTTACCGTGGAGCAGGTATTCACCGGCGGTA
AAAAGCTGGGCGTGCAGTCCGGCACCTCTGAAGCCGACTGGCTGGACAAGGAAAAGCCCAACAAGCCCGAGTGGAATTAC
ACCGTCCGTTTTTACGATTCCGCACCGCTGGCCATTGAAGATGTGATCAACGGCCGCATAGACGCCGCCGCCATGGACAT
CGCCCCTGCGCAGGACGCCCAGCGGGTGAAGCCCATTGAAGTGGTGGGTTCCTTCACCGATCCTGAATATTTCGGCATAG
CCGTACGCAAAGAAGACAAAGAACTGCTGGAAACCATCAACAAGGGACTGGCACTGCTGAAAGCCGACCCCTACTGGGAA
GAGCTGAAAGCCAAGTATCTGGGCGAAAAGCACTGA

Upstream 100 bases:

>100_bases
TCTCGGCATTACGGGAACTTCCGCTCTCAAGGAAGTGGTCACTCCCGTCCGGCTTCGTGCCCGCAGTCAACCCTTTCCAA
CCCCGAAGAAGGATACGAGC

Downstream 100 bases:

>100_bases
TGCCGTAATGCTCTGCCGCATGTTCCGGCCGGTTTACCCGCCGGAACATGCGGCAAAACAGCTATCGCCTTCCGGACGAA
ATTTCTGTATAGCGGACAGG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLRKFTIMAAVLALSLAFAGTAFAGKTLINGIDANYPPFAYVDKDGKPAGFDVDAMEWIAAKMGFTVKHKPMEWSTIVQS
LVSKKIDMVCSGMSITEERARQVSFSDPYWTVAQVFIAKKGSGLTVEQVFTGGKKLGVQSGTSEADWLDKEKPNKPEWNY
TVRFYDSAPLAIEDVINGRIDAAAMDIAPAQDAQRVKPIEVVGSFTDPEYFGIAVRKEDKELLETINKGLALLKADPYWE
ELKAKYLGEKH

Sequences:

>Translated_251_residues
MLRKFTIMAAVLALSLAFAGTAFAGKTLINGIDANYPPFAYVDKDGKPAGFDVDAMEWIAAKMGFTVKHKPMEWSTIVQS
LVSKKIDMVCSGMSITEERARQVSFSDPYWTVAQVFIAKKGSGLTVEQVFTGGKKLGVQSGTSEADWLDKEKPNKPEWNY
TVRFYDSAPLAIEDVINGRIDAAAMDIAPAQDAQRVKPIEVVGSFTDPEYFGIAVRKEDKELLETINKGLALLKADPYWE
ELKAKYLGEKH
>Mature_251_residues
MLRKFTIMAAVLALSLAFAGTAFAGKTLINGIDANYPPFAYVDKDGKPAGFDVDAMEWIAAKMGFTVKHKPMEWSTIVQS
LVSKKIDMVCSGMSITEERARQVSFSDPYWTVAQVFIAKKGSGLTVEQVFTGGKKLGVQSGTSEADWLDKEKPNKPEWNY
TVRFYDSAPLAIEDVINGRIDAAAMDIAPAQDAQRVKPIEVVGSFTDPEYFGIAVRKEDKELLETINKGLALLKADPYWE
ELKAKYLGEKH

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=242, Percent_Identity=34.7107438016529, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=32.0987654320988, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788228, Length=225, Percent_Identity=32.4444444444444, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=29.6943231441048, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=28.0632411067194, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27741; Mature: 27741

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKFTIMAAVLALSLAFAGTAFAGKTLINGIDANYPPFAYVDKDGKPAGFDVDAMEWIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
AKMGFTVKHKPMEWSTIVQSLVSKKIDMVCSGMSITEERARQVSFSDPYWTVAQVFIAKK
HHHCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
GSGLTVEQVFTGGKKLGVQSGTSEADWLDKEKPNKPEWNYTVRFYDSAPLAIEDVINGRI
CCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCC
DAAAMDIAPAQDAQRVKPIEVVGSFTDPEYFGIAVRKEDKELLETINKGLALLKADPYWE
CHHHCCCCCCCCHHHCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHCEEEEECCCCHH
ELKAKYLGEKH
HHHHHHCCCCC
>Mature Secondary Structure
MLRKFTIMAAVLALSLAFAGTAFAGKTLINGIDANYPPFAYVDKDGKPAGFDVDAMEWIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
AKMGFTVKHKPMEWSTIVQSLVSKKIDMVCSGMSITEERARQVSFSDPYWTVAQVFIAKK
HHHCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCHHHHHHHHHHCC
GSGLTVEQVFTGGKKLGVQSGTSEADWLDKEKPNKPEWNYTVRFYDSAPLAIEDVINGRI
CCCCCHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCC
DAAAMDIAPAQDAQRVKPIEVVGSFTDPEYFGIAVRKEDKELLETINKGLALLKADPYWE
CHHHCCCCCCCCHHHCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHCEEEEECCCCHH
ELKAKYLGEKH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]