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The map label for this gene is glnH [H]

Identifier: 78355215

GI number: 78355215

Start: 152033

End: 152776

Strand: Reverse

Name: glnH [H]

Synonym: Dde_0168

Alternate gene names: 78355215

Gene position: 152776-152033 (Counterclockwise)

Preceding gene: 78355217

Following gene: 78355214

Centisome position: 4.1

GC content: 51.61

Gene sequence:

>744_bases
ATGAAGCGCGTTATTCTGGCAGCTTTTGCTCTTGTTATGCTTCTTTCGGCCAACGCATGGGCGCAGAAGCTCGTTGTTGC
GCACGACACCAATTTCAAACCTTTCGAGTTCAAAGACGAAACCGGAAACTACACCGGATTCGACATCGAACTGTGGAAGG
CCATCGCCAGCGAAATCGGTGTGGAATACACCTTCCAGCCCAACGACTTCAACGGAATCATTCCCGGTCTGCAGAGTGCC
ACTTTTGATGCAGGCATCGCCGGCATCACCATCAAGCCCGAACGGCAGGAAGTAGTTGATTTTTCCGACCCGTACTACGA
TTCAGGTCTCGTTATTCTTGTCCGTGCCGACAATAACGACATCAAAGGCATCGAAGACCTGAAAGACAAAGTCATTTCCA
CCAAGCTGGCCACTTCCAGCGCAGACTTTGCCGCCGGTTTTGCTCCCAAAGACAATATCAAGCTGTACCCCAACAACGAC
GCCATGTTCCTTGAACTGCTTTCCGGCGGTGCCGATGCAGTTATTTTTGACATGCCCGTGGTCAAAGACTTTGCCCAGAA
AGCAGGCAAAGGTCAGGTTAAAGTTGTGGGCCCCCTGTATCAGGGGCAGTCTTACGGCATTGCTTTCCCCAAGGGCAGCG
ACCTGAAGCCCAAAGTGGACGCTGCGCTTAAAAAGCTGCGTGAAAACGGCACGTACGAAAAACTGTACATCAAGTGGTTC
GGCTACGCCCCTGCAAAAAAGTAG

Upstream 100 bases:

>100_bases
TGAGCACAGCCTACTGTTTCCCGTCACTCGGCCGGTACCTGCACCGGACCGGCACATGTATACCCGGAATGCCGGGACTC
ACCCATCCAGGAGGTTCATC

Downstream 100 bases:

>100_bases
TCTTTACAGTTTGTAAATAATAACCGCCCGGACATGCATAGGGATGGCGCGCGCCATCCCTATGCCGTTTTTCATGCACC
GCCATGCGGATGCGACACAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKRVILAAFALVMLLSANAWAQKLVVAHDTNFKPFEFKDETGNYTGFDIELWKAIASEIGVEYTFQPNDFNGIIPGLQSA
TFDAGIAGITIKPERQEVVDFSDPYYDSGLVILVRADNNDIKGIEDLKDKVISTKLATSSADFAAGFAPKDNIKLYPNND
AMFLELLSGGADAVIFDMPVVKDFAQKAGKGQVKVVGPLYQGQSYGIAFPKGSDLKPKVDAALKKLRENGTYEKLYIKWF
GYAPAKK

Sequences:

>Translated_247_residues
MKRVILAAFALVMLLSANAWAQKLVVAHDTNFKPFEFKDETGNYTGFDIELWKAIASEIGVEYTFQPNDFNGIIPGLQSA
TFDAGIAGITIKPERQEVVDFSDPYYDSGLVILVRADNNDIKGIEDLKDKVISTKLATSSADFAAGFAPKDNIKLYPNND
AMFLELLSGGADAVIFDMPVVKDFAQKAGKGQVKVVGPLYQGQSYGIAFPKGSDLKPKVDAALKKLRENGTYEKLYIKWF
GYAPAKK
>Mature_247_residues
MKRVILAAFALVMLLSANAWAQKLVVAHDTNFKPFEFKDETGNYTGFDIELWKAIASEIGVEYTFQPNDFNGIIPGLQSA
TFDAGIAGITIKPERQEVVDFSDPYYDSGLVILVRADNNDIKGIEDLKDKVISTKLATSSADFAAGFAPKDNIKLYPNND
AMFLELLSGGADAVIFDMPVVKDFAQKAGKGQVKVVGPLYQGQSYGIAFPKGSDLKPKVDAALKKLRENGTYEKLYIKWF
GYAPAKK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=49.7942386831276, Blast_Score=240, Evalue=6e-65,
Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=31.9672131147541, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=33.0612244897959, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788228, Length=247, Percent_Identity=29.9595141700405, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1788648, Length=236, Percent_Identity=31.3559322033898, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=29.6153846153846, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27093; Mature: 27093

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVILAAFALVMLLSANAWAQKLVVAHDTNFKPFEFKDETGNYTGFDIELWKAIASEIG
CCHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHC
VEYTFQPNDFNGIIPGLQSATFDAGIAGITIKPERQEVVDFSDPYYDSGLVILVRADNND
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEEEECCCC
IKGIEDLKDKVISTKLATSSADFAAGFAPKDNIKLYPNNDAMFLELLSGGADAVIFDMPV
CCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEECHH
VKDFAQKAGKGQVKVVGPLYQGQSYGIAFPKGSDLKPKVDAALKKLRENGTYEKLYIKWF
HHHHHHHCCCCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEE
GYAPAKK
CCCCCCC
>Mature Secondary Structure
MKRVILAAFALVMLLSANAWAQKLVVAHDTNFKPFEFKDETGNYTGFDIELWKAIASEIG
CCHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHC
VEYTFQPNDFNGIIPGLQSATFDAGIAGITIKPERQEVVDFSDPYYDSGLVILVRADNND
EEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCEEEEEEECCCC
IKGIEDLKDKVISTKLATSSADFAAGFAPKDNIKLYPNNDAMFLELLSGGADAVIFDMPV
CCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEECHH
VKDFAQKAGKGQVKVVGPLYQGQSYGIAFPKGSDLKPKVDAALKKLRENGTYEKLYIKWF
HHHHHHHCCCCCEEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEE
GYAPAKK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]