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The map label for this gene is asnC

Identifier: 78355146

GI number: 78355146

Start: 89444

End: 90829

Strand: Reverse

Name: asnC

Synonym: Dde_0099

Alternate gene names: 78355146

Gene position: 90829-89444 (Counterclockwise)

Preceding gene: 78355148

Following gene: 78355145

Centisome position: 2.43

GC content: 57.94

Gene sequence:

>1386_bases
ATGAAACGTACGCAGATACTCGACGCCCTCAACGCATCCGCCCCCTGCGACGATATCCGCATCTGCGGATGGGTGCGCAC
CCGCCGTGATGCCAAAGGCTTTTCCTTTCTCGAACTCAACGACGGGTCGTGCCTGACCAATATACAGGTTATCGTCGATG
AAGAGACCGATGCCTACCGTTCGGTGGGAGAAGCCACCACGGGCGCCTCGGTGGACATCCGCGGGCAGCTGGTGGAATCG
CCGGGCAAAGGGCAGAAATGGGAAGTGCGCGCCGCAACCATGACGCTGCTGGGCAAAGCCGACCCCGAAACTTTTCCGCT
GCAGAAAAAACGCCATTCCGACGAATACCTGCGCACCATCGCGCATCTGCGTCCGCGCACCAACAAATTTGGCGCCGCGT
TCCGCATCCGCGCGGAAGCAAGCTACGCGGTGCACTCATTTTTCCGCGAAAACGGCTTTTTCAACGTGCACACGCCCATT
CTCACCGGTTCGGACTGTGAAGGCGCCGGAGAAATGTTCCGGGTGACCACCCTGCCCGCGGTGGGCGCATCGGCACCCGC
AGAAGGTTCGGTGTTTGACGGGGACTTTTTCGGCAAGGAATGCAGCCTGACCGTTTCCGGTCAGCTGGAAGCTGAAATAA
TGGCACAGAGTCTCGGCAAGGTTTACACCTTCGGCCCCACCTTCCGCGCCGAAAATTCCAACACTCCGCGCCATGCCGCC
GAATTCTGGATGGTCGAGCCGGAAATGGCCTTTGCCGACCTTGAAGACGACATGCAGCTGGCAGAAGACATGGTCAAGTA
TGTAATCAGCCATGTGATGAAGCATTGCGCAGCTGACCTGGACCTGTTCTGCAAGTTTGTGGATAAAGAGCTTTCCGCGC
GGCTTGAAAACATTCTCGCCCACCCCTTTGCCCGCGTCAGCTACACGGAAGCCATCGAAATCCTGAAAGCCAGCGGCAGA
AAATTTGAATACCCCGCCGAGTGGGGCGCAGATCTGCAGACCGAACATGAGCGCTATCTGGCCGAAGAACACTTTAAAAA
GCCTGTGGCTGTCTATGACTACCCCAAAGAAATCAAGGCTTTTTACATGCGTCTGAATGATGACGGCAAAACCGTGGCCG
CCATGGACCTGCTGGTGCCGCGCATCGGCGAACTGGTTGGCGGTTCGCAGCGCGAAGAGCGGCTGGACGTGCTGGAAGCC
CGCATAAAAGAACTGGGCCAGAATCCTGATGATTACTGGTGGTACCTGGAACTGCGCAAATTCGGCACCGCTCCCCACGC
CGGCTTCGGCATGGGGTTTGAGCGTCTGCTCATGCTGCTTACCGGCATCGGCAACATCCGCGACGTCATTCCCTTTCCCC
GTACGCCGCGGCATCTGGAATTTTAG

Upstream 100 bases:

>100_bases
TGACCGCAACCACCAATCCGCCTATCCCTTCGCAAGAAGCAGCAGCCGCGCACAGACAGGCCGCCCATTGTTCTACCAGC
CACGCGAAGGAGACTCTTTT

Downstream 100 bases:

>100_bases
CCTCTCCGGCAGCCCGTACATAAAAAAAGCTCTGCATCTGCAGAGCTTTTTTTGTGCGTACTCCCTGAATTTCCGGAATC
CGTTGCCATAGCCCTACAGA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKRTQILDALNASAPCDDIRICGWVRTRRDAKGFSFLELNDGSCLTNIQVIVDEETDAYRSVGEATTGASVDIRGQLVES
PGKGQKWEVRAATMTLLGKADPETFPLQKKRHSDEYLRTIAHLRPRTNKFGAAFRIRAEASYAVHSFFRENGFFNVHTPI
LTGSDCEGAGEMFRVTTLPAVGASAPAEGSVFDGDFFGKECSLTVSGQLEAEIMAQSLGKVYTFGPTFRAENSNTPRHAA
EFWMVEPEMAFADLEDDMQLAEDMVKYVISHVMKHCAADLDLFCKFVDKELSARLENILAHPFARVSYTEAIEILKASGR
KFEYPAEWGADLQTEHERYLAEEHFKKPVAVYDYPKEIKAFYMRLNDDGKTVAAMDLLVPRIGELVGGSQREERLDVLEA
RIKELGQNPDDYWWYLELRKFGTAPHAGFGMGFERLLMLLTGIGNIRDVIPFPRTPRHLEF

Sequences:

>Translated_461_residues
MKRTQILDALNASAPCDDIRICGWVRTRRDAKGFSFLELNDGSCLTNIQVIVDEETDAYRSVGEATTGASVDIRGQLVES
PGKGQKWEVRAATMTLLGKADPETFPLQKKRHSDEYLRTIAHLRPRTNKFGAAFRIRAEASYAVHSFFRENGFFNVHTPI
LTGSDCEGAGEMFRVTTLPAVGASAPAEGSVFDGDFFGKECSLTVSGQLEAEIMAQSLGKVYTFGPTFRAENSNTPRHAA
EFWMVEPEMAFADLEDDMQLAEDMVKYVISHVMKHCAADLDLFCKFVDKELSARLENILAHPFARVSYTEAIEILKASGR
KFEYPAEWGADLQTEHERYLAEEHFKKPVAVYDYPKEIKAFYMRLNDDGKTVAAMDLLVPRIGELVGGSQREERLDVLEA
RIKELGQNPDDYWWYLELRKFGTAPHAGFGMGFERLLMLLTGIGNIRDVIPFPRTPRHLEF
>Mature_461_residues
MKRTQILDALNASAPCDDIRICGWVRTRRDAKGFSFLELNDGSCLTNIQVIVDEETDAYRSVGEATTGASVDIRGQLVES
PGKGQKWEVRAATMTLLGKADPETFPLQKKRHSDEYLRTIAHLRPRTNKFGAAFRIRAEASYAVHSFFRENGFFNVHTPI
LTGSDCEGAGEMFRVTTLPAVGASAPAEGSVFDGDFFGKECSLTVSGQLEAEIMAQSLGKVYTFGPTFRAENSNTPRHAA
EFWMVEPEMAFADLEDDMQLAEDMVKYVISHVMKHCAADLDLFCKFVDKELSARLENILAHPFARVSYTEAIEILKASGR
KFEYPAEWGADLQTEHERYLAEEHFKKPVAVYDYPKEIKAFYMRLNDDGKTVAAMDLLVPRIGELVGGSQREERLDVLEA
RIKELGQNPDDYWWYLELRKFGTAPHAGFGMGFERLLMLLTGIGNIRDVIPFPRTPRHLEF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=459, Percent_Identity=47.276688453159, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI4758762, Length=445, Percent_Identity=31.0112359550562, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI45439306, Length=344, Percent_Identity=27.906976744186, Blast_Score=115, Evalue=8e-26,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=56.6523605150215, Blast_Score=539, Evalue=1e-154,
Organism=Escherichia coli, GI1790571, Length=480, Percent_Identity=22.5, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788173, Length=275, Percent_Identity=24, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556372, Length=420, Percent_Identity=33.5714285714286, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71984122, Length=449, Percent_Identity=31.8485523385301, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17551876, Length=489, Percent_Identity=27.19836400818, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319872, Length=470, Percent_Identity=38.5106382978723, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6321807, Length=461, Percent_Identity=29.2841648590022, Blast_Score=185, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=352, Percent_Identity=30.1136363636364, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922416, Length=464, Percent_Identity=37.0689655172414, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI19921528, Length=445, Percent_Identity=29.6629213483146, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17136276, Length=351, Percent_Identity=26.4957264957265, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_DESDG (Q317J6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_386595.1
- HSSP:   O57980
- ProteinModelPortal:   Q317J6
- SMR:   Q317J6
- STRING:   Q317J6
- GeneID:   3758841
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_0099
- NMPDR:   fig|207559.3.peg.755
- eggNOG:   COG0017
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   DDES207559:DDE_0099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 51878; Mature: 51878

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTQILDALNASAPCDDIRICGWVRTRRDAKGFSFLELNDGSCLTNIQVIVDEETDAYR
CCHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCEEEEEEEEEECCCHHHH
SVGEATTGASVDIRGQLVESPGKGQKWEVRAATMTLLGKADPETFPLQKKRHSDEYLRTI
HHCCCCCCCCEEEEEEEECCCCCCCCEEEEEHHEEECCCCCCCCCCCHHHCCHHHHHHHH
AHLRPRTNKFGAAFRIRAEASYAVHSFFRENGFFNVHTPILTGSDCEGAGEMFRVTTLPA
HHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEECCEEECCCCCCCCCEEEEEECCC
VGASAPAEGSVFDGDFFGKECSLTVSGQLEAEIMAQSLGKVYTFGPTFRAENSNTPRHAA
CCCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHCCEEECCCCEECCCCCCCCHHH
EFWMVEPEMAFADLEDDMQLAEDMVKYVISHVMKHCAADLDLFCKFVDKELSARLENILA
HHEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPFARVSYTEAIEILKASGRKFEYPAEWGADLQTEHERYLAEEHFKKPVAVYDYPKEIKA
CCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHH
FYMRLNDDGKTVAAMDLLVPRIGELVGGSQREERLDVLEARIKELGQNPDDYWWYLELRK
EEEEECCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
FGTAPHAGFGMGFERLLMLLTGIGNIRDVIPFPRTPRHLEF
CCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRTQILDALNASAPCDDIRICGWVRTRRDAKGFSFLELNDGSCLTNIQVIVDEETDAYR
CCHHHHHHHHCCCCCCCCEEEEHHHHHCCCCCCCEEEEECCCCEEEEEEEEEECCCHHHH
SVGEATTGASVDIRGQLVESPGKGQKWEVRAATMTLLGKADPETFPLQKKRHSDEYLRTI
HHCCCCCCCCEEEEEEEECCCCCCCCEEEEEHHEEECCCCCCCCCCCHHHCCHHHHHHHH
AHLRPRTNKFGAAFRIRAEASYAVHSFFRENGFFNVHTPILTGSDCEGAGEMFRVTTLPA
HHHCCCCCCCCCEEEEEECCHHHHHHHHHHCCCEEEECCEEECCCCCCCCCEEEEEECCC
VGASAPAEGSVFDGDFFGKECSLTVSGQLEAEIMAQSLGKVYTFGPTFRAENSNTPRHAA
CCCCCCCCCCEECCCCCCCEEEEEECCCHHHHHHHHHHCCEEECCCCEECCCCCCCCHHH
EFWMVEPEMAFADLEDDMQLAEDMVKYVISHVMKHCAADLDLFCKFVDKELSARLENILA
HHEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPFARVSYTEAIEILKASGRKFEYPAEWGADLQTEHERYLAEEHFKKPVAVYDYPKEIKA
CCHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHH
FYMRLNDDGKTVAAMDLLVPRIGELVGGSQREERLDVLEARIKELGQNPDDYWWYLELRK
EEEEECCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
FGTAPHAGFGMGFERLLMLLTGIGNIRDVIPFPRTPRHLEF
CCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA