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The map label for this gene is capI [H]

Identifier: 78355080

GI number: 78355080

Start: 26246

End: 27343

Strand: Reverse

Name: capI [H]

Synonym: Dde_0033

Alternate gene names: 78355080

Gene position: 27343-26246 (Counterclockwise)

Preceding gene: 78355081

Following gene: 78355079

Centisome position: 0.73

GC content: 53.01

Gene sequence:

>1098_bases
GTGACGGCGGGGGACTTTTGCTCTATATTTCCGCCTGTCATTTTTTACGCACACAGACTGCGCAGCGGCGCAGCAGAACA
GGAATCAGACATGCATATACTAGTCACCGGCGCAGCCGGATTCATAGGGTTTCACCTTTCCAGACGTTTTCTTGCCGAAG
GGCATACTGTTGTAGGCCTTGACTGCCTGAATGATTACTACGACGTACAGCTGAAAAAAGACCGCCTTGCCCTTTTGCAG
CAGGAGAAGGGCTTTACTTTTACGCCCACAGATCTTGCCGACGACGCGGCCATGGACGCGCTTTTTGCCAGAGAACAGTT
CACGCATGTAGTCAATCTGGCTGCTCAGGCCGGAGTCCGCTACAGTATCGAAAACCCGCGTTCATATATCCAGTCCAACC
TTGTGGGCTTCGGCAACATAATTGAAGGTTGCCGCCACAATGGGGTGAAGCATCTTGTGTATGCCTCGTCCAGCTCTGTT
TACGGTCTGAACACCAATATGCCTTTCAGCGTACACGACAACGTGGACCATCCCATCAGCTTGTACGCCGCCAGCAAAAA
AGCCAATGAACTGATGGCTCACACCTACAGCCATCTTTACAGACTGCCTACCACAGGGCTTCGGTTCTTTACCGTGTACG
GCCCGTGGGGCAGACCGGACATGGCTCTTTTTCTGTTCACCAAAGCCATTCTGGAAGGCAAGCCCATCAATGTGTTCAAC
GAGGGGCACATGCGCCGCGACTTCACGTACATTGATGACATCATCGAAGGAGTGGTGCGCGTAACCATGCGCACTCCCGA
GCCCAACCCCCAATGGGACGGAACAGCTCCTGACCCTTCTTCCAGCCCTGCGCCATACCGCATCTACAACATTGGCAACA
ACAACACGGTAGAGCTTGGCGAGTTCATCGCGACTCTGGAAGAATGCCTGGGCAAAAAGGCCGTTAAAAACATGATGCCC
ATGCAGCCCGGCGATGTGGAAGCAACATATGCCAATGTGGACGACCTGATTGCCGACACCGGCTTCAAGCCCGGAACTCC
GCTCAAAGAAGGGATAGCAAACTTTGTGAGCTGGTATCGGGAGTACTACAAAAAATAG

Upstream 100 bases:

>100_bases
GCTTCCGGCATGAACGTAAAATTTTTCATGCGTACCGTAGGCGGCGATACCCTCTGCCACCAGCATTTTTCATCCGTCCG
GATTACCTTCAGGGCAAAAG

Downstream 100 bases:

>100_bases
CTGCCGTGTACCGCACCAGACTTCAGGCCCGCTTTTGCGGGCTTTTTTGTTGCTGCATGGTCTCGCTCAACGTATTTCTT
TTTGTAATACAAAAAGACTT

Product: NAD-dependent epimerase/dehydratase family protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTAGDFCSIFPPVIFYAHRLRSGAAEQESDMHILVTGAAGFIGFHLSRRFLAEGHTVVGLDCLNDYYDVQLKKDRLALLQ
QEKGFTFTPTDLADDAAMDALFAREQFTHVVNLAAQAGVRYSIENPRSYIQSNLVGFGNIIEGCRHNGVKHLVYASSSSV
YGLNTNMPFSVHDNVDHPISLYAASKKANELMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGKPINVFN
EGHMRRDFTYIDDIIEGVVRVTMRTPEPNPQWDGTAPDPSSSPAPYRIYNIGNNNTVELGEFIATLEECLGKKAVKNMMP
MQPGDVEATYANVDDLIADTGFKPGTPLKEGIANFVSWYREYYKK

Sequences:

>Translated_365_residues
MTAGDFCSIFPPVIFYAHRLRSGAAEQESDMHILVTGAAGFIGFHLSRRFLAEGHTVVGLDCLNDYYDVQLKKDRLALLQ
QEKGFTFTPTDLADDAAMDALFAREQFTHVVNLAAQAGVRYSIENPRSYIQSNLVGFGNIIEGCRHNGVKHLVYASSSSV
YGLNTNMPFSVHDNVDHPISLYAASKKANELMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGKPINVFN
EGHMRRDFTYIDDIIEGVVRVTMRTPEPNPQWDGTAPDPSSSPAPYRIYNIGNNNTVELGEFIATLEECLGKKAVKNMMP
MQPGDVEATYANVDDLIADTGFKPGTPLKEGIANFVSWYREYYKK
>Mature_364_residues
TAGDFCSIFPPVIFYAHRLRSGAAEQESDMHILVTGAAGFIGFHLSRRFLAEGHTVVGLDCLNDYYDVQLKKDRLALLQQ
EKGFTFTPTDLADDAAMDALFAREQFTHVVNLAAQAGVRYSIENPRSYIQSNLVGFGNIIEGCRHNGVKHLVYASSSSVY
GLNTNMPFSVHDNVDHPISLYAASKKANELMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGKPINVFNE
GHMRRDFTYIDDIIEGVVRVTMRTPEPNPQWDGTAPDPSSSPAPYRIYNIGNNNTVELGEFIATLEECLGKKAVKNMMPM
QPGDVEATYANVDDLIADTGFKPGTPLKEGIANFVSWYREYYKK

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=26.0355029585799, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI56237023, Length=350, Percent_Identity=24.8571428571429, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56118217, Length=350, Percent_Identity=24.8571428571429, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI189083684, Length=350, Percent_Identity=24.8571428571429, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI42516563, Length=362, Percent_Identity=22.9281767955801, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=25.8426966292135, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994969, Length=331, Percent_Identity=27.7945619335347, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=22.7011494252874, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982035, Length=351, Percent_Identity=25.6410256410256, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=25.4957507082153, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17568069, Length=358, Percent_Identity=25.4189944134078, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=341, Percent_Identity=22.8739002932551, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19923002, Length=341, Percent_Identity=24.633431085044, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=23.3532934131737, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 40791; Mature: 40659

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGDFCSIFPPVIFYAHRLRSGAAEQESDMHILVTGAAGFIGFHLSRRFLAEGHTVVGL
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEH
DCLNDYYDVQLKKDRLALLQQEKGFTFTPTDLADDAAMDALFAREQFTHVVNLAAQAGVR
HHHCCCCEEEECHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
YSIENPRSYIQSNLVGFGNIIEGCRHNGVKHLVYASSSSVYGLNTNMPFSVHDNVDHPIS
EECCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEECCCCCCCEE
LYAASKKANELMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGKPINVFN
EEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEC
EGHMRRDFTYIDDIIEGVVRVTMRTPEPNPQWDGTAPDPSSSPAPYRIYNIGNNNTVELG
CCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHH
EFIATLEECLGKKAVKNMMPMQPGDVEATYANVDDLIADTGFKPGTPLKEGIANFVSWYR
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EYYKK
HHHCC
>Mature Secondary Structure 
TAGDFCSIFPPVIFYAHRLRSGAAEQESDMHILVTGAAGFIGFHLSRRFLAEGHTVVGL
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEH
DCLNDYYDVQLKKDRLALLQQEKGFTFTPTDLADDAAMDALFAREQFTHVVNLAAQAGVR
HHHCCCCEEEECHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
YSIENPRSYIQSNLVGFGNIIEGCRHNGVKHLVYASSSSVYGLNTNMPFSVHDNVDHPIS
EECCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEECCCCCCCEE
LYAASKKANELMAHTYSHLYRLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGKPINVFN
EEEHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEC
EGHMRRDFTYIDDIIEGVVRVTMRTPEPNPQWDGTAPDPSSSPAPYRIYNIGNNNTVELG
CCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEHHH
EFIATLEECLGKKAVKNMMPMQPGDVEATYANVDDLIADTGFKPGTPLKEGIANFVSWYR
HHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHHH
EYYKK
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]