Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 78355060

GI number: 78355060

Start: 13700

End: 14209

Strand: Direct

Name: def [H]

Synonym: Dde_0013

Alternate gene names: 78355060

Gene position: 13700-14209 (Clockwise)

Preceding gene: 78355059

Following gene: 78355061

Centisome position: 0.37

GC content: 54.71

Gene sequence:

>510_bases
ATGATTCGAGAAATTCTGACATACCCCGACAGACGCCTTGCCATCGAATGCGAGGAAATTGAAGAGATAACAGACGAGAT
ACGTCAGCTGGCAGCCGATATGGCTGAAACGATGTATGACGCAGACGGTATCGGTCTGGCTGCGCCGCAGGTGGGTGCCA
CCTGCCGGCTTATTGTGGTCGATGTTTCCGGTCCGGAGGCGCGCGAGGACCTGCGGACATACATTAATCCGCGTCTTGAG
CTGCTGGAGGGCAAGGTTGATACCGAAGAGGGCTGCCTGTCCGTGCCTGCACTGCGCAGCAAGGTAACCCGTACTGAAAA
GGTACGTCTGCACGCCACCGACCTTGACGGCAACGCCGTGTGCATTGACGCCGACGGACTGCTTTCCATCTGTCTGCAGC
ACGAAATAGACCATCTGGACGGTACGCTGTTTATCGACAAGATAAGCCGCCTTAAGCGTTCACTCTATGACAACAAAGTG
AAAAAATGGCAGAAGCGCGCGAAAAACTGA

Upstream 100 bases:

>100_bases
CGGCGGTTGTCCGTGCGGCAGGGGCTTTTCAACGGATGTACTGTGCTTATTATTTGTCTGAAACGCCGTTGTGCGTAAGC
CCGCAGCAGGAAAAAAAGCT

Downstream 100 bases:

>100_bases
AAATAGTCTACATGGGCACGCCGGATTTTGCGGCGACGGTACTTTCTCATCTGCTTGCGTGGGAAGACGCCGAGGTGCTT
GCTGTGTACACGCAGCCCGA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MIREILTYPDRRLAIECEEIEEITDEIRQLAADMAETMYDADGIGLAAPQVGATCRLIVVDVSGPEAREDLRTYINPRLE
LLEGKVDTEEGCLSVPALRSKVTRTEKVRLHATDLDGNAVCIDADGLLSICLQHEIDHLDGTLFIDKISRLKRSLYDNKV
KKWQKRAKN

Sequences:

>Translated_169_residues
MIREILTYPDRRLAIECEEIEEITDEIRQLAADMAETMYDADGIGLAAPQVGATCRLIVVDVSGPEAREDLRTYINPRLE
LLEGKVDTEEGCLSVPALRSKVTRTEKVRLHATDLDGNAVCIDADGLLSICLQHEIDHLDGTLFIDKISRLKRSLYDNKV
KKWQKRAKN
>Mature_169_residues
MIREILTYPDRRLAIECEEIEEITDEIRQLAADMAETMYDADGIGLAAPQVGATCRLIVVDVSGPEAREDLRTYINPRLE
LLEGKVDTEEGCLSVPALRSKVTRTEKVRLHATDLDGNAVCIDADGLLSICLQHEIDHLDGTLFIDKISRLKRSLYDNKV
KKWQKRAKN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=167, Percent_Identity=30.5389221556886, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789682, Length=162, Percent_Identity=46.9135802469136, Blast_Score=140, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24645728, Length=140, Percent_Identity=32.8571428571429, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24645726, Length=142, Percent_Identity=32.3943661971831, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19036; Mature: 19036

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILTYPDRRLAIECEEIEEITDEIRQLAADMAETMYDADGIGLAAPQVGATCRLIVV
CCCCHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DVSGPEAREDLRTYINPRLELLEGKVDTEEGCLSVPALRSKVTRTEKVRLHATDLDGNAV
ECCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCEE
CIDADGLLSICLQHEIDHLDGTLFIDKISRLKRSLYDNKVKKWQKRAKN
EEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIREILTYPDRRLAIECEEIEEITDEIRQLAADMAETMYDADGIGLAAPQVGATCRLIVV
CCCCHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEE
DVSGPEAREDLRTYINPRLELLEGKVDTEEGCLSVPALRSKVTRTEKVRLHATDLDGNAV
ECCCCCHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEEECCCCCEE
CIDADGLLSICLQHEIDHLDGTLFIDKISRLKRSLYDNKVKKWQKRAKN
EEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA