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The map label for this gene is cbiF [H]

Identifier: 304570581

GI number: 304570581

Start: 821057

End: 821869

Strand: Reverse

Name: cbiF [H]

Synonym: NA

Alternate gene names: 304570581

Gene position: 821869-821057 (Counterclockwise)

Preceding gene: 78355849

Following gene: 78355847

Centisome position: 22.03

GC content: 63.59

Gene sequence:

>813_bases
ATGGAGAATAGCGTGACCGCAAACCCGGCGGACCCCAGAGTCTGGTTTATCGGCGCCGGCCCCGGCGACCCCGAGCTGAT
CACCGTAAAGGGGCAGAGAATCATCTCGCAGGCGGATCTGGTGCTGTATGCAGGCTCGCTGGTGCCGCCACAGGTGGTTG
CCTGTGCCCGTCCGGACGCCGTGGTGGCCGACAGTGCGCCCATGAACCTGCAGCAGACACATGCCATGATACGCGAAACC
GTGCACAGCGGGGGTCTAGTGGCCAGAGTCCACACCGGCGACCCGGCCCTGTACGGCGCCATCCGCGAACAGATGGCCCT
GCTGGCGCTGGAAAACATCGCCTGCGGCGTTGTTCCCGGAGTCACATCAGCCTTTGCGGCAGCCGCCGCGGCAGGCGTTT
CGCTCACGCTGCCCGAAATCACCCAGTCGGTGATCATCACCCGCGTGCAGGGGCGCACCCCCGTGCCCGAAGCCGAGCAG
CTGCGGCATCTTGCACGGCGGGGAACAGCCATGGCCATATATCTTTCCGCCGCCCTGCCCGAAACCATAACCGAAGAACT
ACGCGCCGGCGGACTGCCCGAAAGCACCCGCATCATCGCCGCATACAGGGTGGGCTGGCCGGACCAACGTCTGGTGGAAT
GCACGCTGGCAACGCTGACGGACACCGTGCAGCAGGAAAAGCTCAACCGGCAGACGGTCTTTCTTGTCCTGCCAGGCAAT
TCCATGCATAGTGGTATACAGCACCCCGATGCAGCTTCACCGCCGTGTTCCAGACTGTATGATGCAGATTTTTCGCACGG
CTGGCGCAAGTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:
NA

Sequences:

>Translated_270_residues
MENSVTANPADPRVWFIGAGPGDPELITVKGQRIISQADLVLYAGSLVPPQVVACARPDAVVADSAPMNLQQTHAMIRET
VHSGGLVARVHTGDPALYGAIREQMALLALENIACGVVPGVTSAFAAAAAAGVSLTLPEITQSVIITRVQGRTPVPEAEQ
LRHLARRGTAMAIYLSAALPETITEELRAGGLPESTRIIAAYRVGWPDQRLVECTLATLTDTVQQEKLNRQTVFLVLPGN
SMHSGIQHPDAASPPCSRLYDADFSHGWRK
>Mature_270_residues
MENSVTANPADPRVWFIGAGPGDPELITVKGQRIISQADLVLYAGSLVPPQVVACARPDAVVADSAPMNLQQTHAMIRET
VHSGGLVARVHTGDPALYGAIREQMALLALENIACGVVPGVTSAFAAAAAAGVSLTLPEITQSVIITRVQGRTPVPEAEQ
LRHLARRGTAMAIYLSAALPETITEELRAGGLPESTRIIAAYRVGWPDQRLVECTLATLTDTVQQEKLNRQTVFLVLPGN
SMHSGIQHPDAASPPCSRLYDADFSHGWRK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=225, Percent_Identity=29.3333333333333, Blast_Score=81, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28735; Mature: 28735

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENSVTANPADPRVWFIGAGPGDPELITVKGQRIISQADLVLYAGSLVPPQVVACARPDA
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHCCEEEEECCCCCCHHEEECCCCC
VVADSAPMNLQQTHAMIRETVHSGGLVARVHTGDPALYGAIREQMALLALENIACGVVPG
EEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VTSAFAAAAAAGVSLTLPEITQSVIITRVQGRTPVPEAEQLRHLARRGTAMAIYLSAALP
HHHHHHHHHHCCCEEEHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEHHCC
ETITEELRAGGLPESTRIIAAYRVGWPDQRLVECTLATLTDTVQQEKLNRQTVFLVLPGN
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
SMHSGIQHPDAASPPCSRLYDADFSHGWRK
HHHCCCCCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MENSVTANPADPRVWFIGAGPGDPELITVKGQRIISQADLVLYAGSLVPPQVVACARPDA
CCCCCCCCCCCCEEEEEECCCCCCCEEEECCCHHHHHCCEEEEECCCCCCHHEEECCCCC
VVADSAPMNLQQTHAMIRETVHSGGLVARVHTGDPALYGAIREQMALLALENIACGVVPG
EEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VTSAFAAAAAAGVSLTLPEITQSVIITRVQGRTPVPEAEQLRHLARRGTAMAIYLSAALP
HHHHHHHHHHCCCEEEHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCEEEEEEEHHCC
ETITEELRAGGLPESTRIIAAYRVGWPDQRLVECTLATLTDTVQQEKLNRQTVFLVLPGN
HHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
SMHSGIQHPDAASPPCSRLYDADFSHGWRK
HHHCCCCCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9742225; 9660189 [H]