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The map label for this gene is def [H]

Identifier: 78224736

GI number: 78224736

Start: 3987960

End: 3988466

Strand: Reverse

Name: def [H]

Synonym: Gmet_3553

Alternate gene names: 78224736

Gene position: 3988466-3987960 (Counterclockwise)

Preceding gene: 78224737

Following gene: 78224733

Centisome position: 99.78

GC content: 63.71

Gene sequence:

>507_bases
ATGCCCGCACAGCCCATCCTCTGCTATCCCCATCCCGTTCTAAAGAAGGTGAGCCATGCCGTCGCCGTTATCGACGACGA
GATCCGGGGTCTTATCGACGACCTTCTGGACACCATGCGTGCCGGACCCGGCTCCGTCGGGGTCGCCGCACCCCAGATCG
GCGCCACCCTCAGGGTCTGCGTGGTGGACGTCTCCGGAAGCCGTCACGGCAAGGAGAACAACCACGGTGTCCTTGTCATG
GTAAACCCCGAGATCGTCCACCGGGAAGGTGCGGCTATCATGCGGGAGGGATGCATGAGCGTCCCAGACTACACCGGCGA
CGTAGAGCGGGCCACCACCATCACCGTCCGGTTCCGGGACGGGGAGGGGACGGAGCGGGAGATCTCCGCCAGCGGCTTCG
AGGCGGTCGCCATCCAGCACGAGATGGACCATCTGGACGGGATCCTCTTTTTGGATCGGATCGTGTCGCTCAAGACGGGG
CTGTTCCGGAGGAAGAACTACAGGTAG

Upstream 100 bases:

>100_bases
ATTGTCGTGGAAGACGTGGCGGCAATGTTCGACAAGATTAAGAAGGAGATGAACGTGGAGATTTCGGTGCGATCCATCAC
GCCGGTCTCCCTCTGACGCC

Downstream 100 bases:

>100_bases
CGTGAGCTGCCGGATGGATAAAACTAAAGCGGGGAGGCTGCTGGCGCGGCCCCCCCCGAAGACTGGCGTCGTAGTCTGTG
GCGGTTACTTCATCTCTTTC

Product: formylmethionine deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MPAQPILCYPHPVLKKVSHAVAVIDDEIRGLIDDLLDTMRAGPGSVGVAAPQIGATLRVCVVDVSGSRHGKENNHGVLVM
VNPEIVHREGAAIMREGCMSVPDYTGDVERATTITVRFRDGEGTEREISASGFEAVAIQHEMDHLDGILFLDRIVSLKTG
LFRRKNYR

Sequences:

>Translated_168_residues
MPAQPILCYPHPVLKKVSHAVAVIDDEIRGLIDDLLDTMRAGPGSVGVAAPQIGATLRVCVVDVSGSRHGKENNHGVLVM
VNPEIVHREGAAIMREGCMSVPDYTGDVERATTITVRFRDGEGTEREISASGFEAVAIQHEMDHLDGILFLDRIVSLKTG
LFRRKNYR
>Mature_167_residues
PAQPILCYPHPVLKKVSHAVAVIDDEIRGLIDDLLDTMRAGPGSVGVAAPQIGATLRVCVVDVSGSRHGKENNHGVLVMV
NPEIVHREGAAIMREGCMSVPDYTGDVERATTITVRFRDGEGTEREISASGFEAVAIQHEMDHLDGILFLDRIVSLKTGL
FRRKNYR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=165, Percent_Identity=38.1818181818182, Blast_Score=113, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24645726, Length=158, Percent_Identity=32.2784810126582, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24645728, Length=160, Percent_Identity=33.125, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 18341; Mature: 18210

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAQPILCYPHPVLKKVSHAVAVIDDEIRGLIDDLLDTMRAGPGSVGVAAPQIGATLRVC
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VVDVSGSRHGKENNHGVLVMVNPEIVHREGAAIMREGCMSVPDYTGDVERATTITVRFRD
EEEECCCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEEC
GEGTEREISASGFEAVAIQHEMDHLDGILFLDRIVSLKTGLFRRKNYR
CCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PAQPILCYPHPVLKKVSHAVAVIDDEIRGLIDDLLDTMRAGPGSVGVAAPQIGATLRVC
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VVDVSGSRHGKENNHGVLVMVNPEIVHREGAAIMREGCMSVPDYTGDVERATTITVRFRD
EEEECCCCCCCCCCCEEEEEECCCEEECCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEEC
GEGTEREISASGFEAVAIQHEMDHLDGILFLDRIVSLKTGLFRRKNYR
CCCCCCEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA