Click here to switch to the map view.

The map label for this gene is zraS [H]

Identifier: 78224660

GI number: 78224660

Start: 3896539

End: 3897888

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_3474

Alternate gene names: 78224660

Gene position: 3897888-3896539 (Counterclockwise)

Preceding gene: 78224661

Following gene: 78224659

Centisome position: 97.51

GC content: 64.44

Gene sequence:

>1350_bases
GTGCCCAGTGGATGTTCAAAGAGACCTATCATGAAAAACCAACATACATCAAACGTATCGGCCAAGCGCCTCAACTACGT
GGTCGGCGAAGAGCGGCTCCTCAGGGACGTCATCACCGAGACAGAGGTGATCCCGCTCCTCAGGGGTGCCGTACGTGCCG
GCGCCCGTGCCGCGGCCCTCGCCGATGCCGAGGGAACCACTCTCTGGTCCCATGCCGCGCCGACCGACGGAGGGGAAGCT
GCCTCCCTGGGGCTCCGAGGGCCCGCCATCCTCCTGGAGGGGGAGCCGGTGGGGTCGATTCTGGTGAAGGGAGAGAGGGG
AAGGGAGGAGTTCCTGAAGGGGATTGCCGTCATGCTCACGGCTGCGGTGAACCTCCTGGTGGGCTCGAACCTGAAGAGGA
TGCTCACCACCGAGATCCACACGACGGTGGTGAACCAGTCCTACGAGGAGCTTCTGGAAACTAACCGGAAGCTTACTGCC
TCCGAGGCCCGCTATCGGGAGCTGGCGGAGAGCCTGGAGATCAAGGTGGAGGAGCGGACCGCGGAGCTGAAAAAGGCCCT
GGCCCGACTCCTGCAACAGGAGAAGCTCGCCTCGGTGGGGCAGCTGGCGGCTGGCATCGCCCACGAGATCAACAATCCCA
TGGGGTTCGTCACGAGCAACATCCGGACGATGCAGAAGTATGCAGGGCGCTTCGTGGAGATGCTTCAGTTCGCCCGGGAT
CTCTTCCCCGTGGCGGGGGGAGGAGCGGGCGGGGAGCGCTTTGCCGAGAAGTGGCGCCAGTTGAAGCTCGACATGGTACT
TGCCGATGTGGACGAACTTGTATCCCAGAGTCTCGGCGGGGCGGAGCGGGTGAAAAAGATTGTGGCCGACCTGAAGGGGT
TCTCGCACATCGACGACCTAGGGGAAACGGTGGCGGACCTGAACGCCGAGATCGACCGAACCCTCGCCGTGCTGGTCCAC
GAGATCCCGGCGGGGACCGAGATCGCCAGGGAGTACGGCTCCATTCCGGGACTCAGCTGCCGGGCGGGGGAACTCTGCCA
GGTGTTCCTCAACATCATCATGAACGCCTGTCAGGCCCGGAGCGAGGGGCTCAGGCTCACCATCCGCACCGCCCTGGAGG
GGGATGCCATCGCCATAACCATCACCGACAACGGCCCCGGCATCCTGCCGGAGCTCCTGGACCGCGTCTTCGAACCCTTC
TTCACCACCAAGCCGGTGGGCCAGGGGATGGGGATGGGGCTTGCGGTGGCCCACGAGGTAGTAACCGCCCACGGCGGCAC
GGTGGAGGCTGGAAGCACGCCCGAAGGAGGTGCCTCTTTCGTGGTGAGACTGCCGGTGACAGGGGGATAG

Upstream 100 bases:

>100_bases
GCGAAAGATGACATCGGTTGAAGCGACGCAGGGCATAGCTGGATTCGTGTAGGGGCGCCTTCATGGCGCTCTGCTCATGC
GATGCGAGGCGCTATAGAGT

Downstream 100 bases:

>100_bases
CATGGCAAAGTTTTCGGAACTCTTCAAAACCGTTGCTGCCGGCAAGGAGCCCCCGCCGCCCCTTTTGCCCGATGACGACG
CCTCGCTGGTGGAGTACAGC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MPSGCSKRPIMKNQHTSNVSAKRLNYVVGEERLLRDVITETEVIPLLRGAVRAGARAAALADAEGTTLWSHAAPTDGGEA
ASLGLRGPAILLEGEPVGSILVKGERGREEFLKGIAVMLTAAVNLLVGSNLKRMLTTEIHTTVVNQSYEELLETNRKLTA
SEARYRELAESLEIKVEERTAELKKALARLLQQEKLASVGQLAAGIAHEINNPMGFVTSNIRTMQKYAGRFVEMLQFARD
LFPVAGGGAGGERFAEKWRQLKLDMVLADVDELVSQSLGGAERVKKIVADLKGFSHIDDLGETVADLNAEIDRTLAVLVH
EIPAGTEIAREYGSIPGLSCRAGELCQVFLNIIMNACQARSEGLRLTIRTALEGDAIAITITDNGPGILPELLDRVFEPF
FTTKPVGQGMGMGLAVAHEVVTAHGGTVEAGSTPEGGASFVVRLPVTGG

Sequences:

>Translated_449_residues
MPSGCSKRPIMKNQHTSNVSAKRLNYVVGEERLLRDVITETEVIPLLRGAVRAGARAAALADAEGTTLWSHAAPTDGGEA
ASLGLRGPAILLEGEPVGSILVKGERGREEFLKGIAVMLTAAVNLLVGSNLKRMLTTEIHTTVVNQSYEELLETNRKLTA
SEARYRELAESLEIKVEERTAELKKALARLLQQEKLASVGQLAAGIAHEINNPMGFVTSNIRTMQKYAGRFVEMLQFARD
LFPVAGGGAGGERFAEKWRQLKLDMVLADVDELVSQSLGGAERVKKIVADLKGFSHIDDLGETVADLNAEIDRTLAVLVH
EIPAGTEIAREYGSIPGLSCRAGELCQVFLNIIMNACQARSEGLRLTIRTALEGDAIAITITDNGPGILPELLDRVFEPF
FTTKPVGQGMGMGLAVAHEVVTAHGGTVEAGSTPEGGASFVVRLPVTGG
>Mature_448_residues
PSGCSKRPIMKNQHTSNVSAKRLNYVVGEERLLRDVITETEVIPLLRGAVRAGARAAALADAEGTTLWSHAAPTDGGEAA
SLGLRGPAILLEGEPVGSILVKGERGREEFLKGIAVMLTAAVNLLVGSNLKRMLTTEIHTTVVNQSYEELLETNRKLTAS
EARYRELAESLEIKVEERTAELKKALARLLQQEKLASVGQLAAGIAHEINNPMGFVTSNIRTMQKYAGRFVEMLQFARDL
FPVAGGGAGGERFAEKWRQLKLDMVLADVDELVSQSLGGAERVKKIVADLKGFSHIDDLGETVADLNAEIDRTLAVLVHE
IPAGTEIAREYGSIPGLSCRAGELCQVFLNIIMNACQARSEGLRLTIRTALEGDAIAITITDNGPGILPELLDRVFEPFF
TTKPVGQGMGMGLAVAHEVVTAHGGTVEAGSTPEGGASFVVRLPVTGG

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=259, Percent_Identity=32.4324324324324, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1788549, Length=270, Percent_Identity=30.3703703703704, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790300, Length=275, Percent_Identity=29.4545454545455, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=100, Percent_Identity=35, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081816, Length=100, Percent_Identity=35, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786912, Length=101, Percent_Identity=34.6534653465347, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788393, Length=106, Percent_Identity=32.0754716981132, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47993; Mature: 47862

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSGCSKRPIMKNQHTSNVSAKRLNYVVGEERLLRDVITETEVIPLLRGAVRAGARAAAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADAEGTTLWSHAAPTDGGEAASLGLRGPAILLEGEPVGSILVKGERGREEFLKGIAVMLT
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHH
AAVNLLVGSNLKRMLTTEIHTTVVNQSYEELLETNRKLTASEARYRELAESLEIKVEERT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AELKKALARLLQQEKLASVGQLAAGIAHEINNPMGFVTSNIRTMQKYAGRFVEMLQFARD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPVAGGGAGGERFAEKWRQLKLDMVLADVDELVSQSLGGAERVKKIVADLKGFSHIDDL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
GETVADLNAEIDRTLAVLVHEIPAGTEIAREYGSIPGLSCRAGELCQVFLNIIMNACQAR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SEGLRLTIRTALEGDAIAITITDNGPGILPELLDRVFEPFFTTKPVGQGMGMGLAVAHEV
CCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VTAHGGTVEAGSTPEGGASFVVRLPVTGG
HHHCCCEEECCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
PSGCSKRPIMKNQHTSNVSAKRLNYVVGEERLLRDVITETEVIPLLRGAVRAGARAAAL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ADAEGTTLWSHAAPTDGGEAASLGLRGPAILLEGEPVGSILVKGERGREEFLKGIAVMLT
CCCCCCEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHH
AAVNLLVGSNLKRMLTTEIHTTVVNQSYEELLETNRKLTASEARYRELAESLEIKVEERT
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AELKKALARLLQQEKLASVGQLAAGIAHEINNPMGFVTSNIRTMQKYAGRFVEMLQFARD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPVAGGGAGGERFAEKWRQLKLDMVLADVDELVSQSLGGAERVKKIVADLKGFSHIDDL
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHH
GETVADLNAEIDRTLAVLVHEIPAGTEIAREYGSIPGLSCRAGELCQVFLNIIMNACQAR
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SEGLRLTIRTALEGDAIAITITDNGPGILPELLDRVFEPFFTTKPVGQGMGMGLAVAHEV
CCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
VTAHGGTVEAGSTPEGGASFVVRLPVTGG
HHHCCCEEECCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]