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The map label for this gene is treZ [H]

Identifier: 78224654

GI number: 78224654

Start: 3882655

End: 3884493

Strand: Direct

Name: treZ [H]

Synonym: Gmet_3467

Alternate gene names: 78224654

Gene position: 3882655-3884493 (Clockwise)

Preceding gene: 78224646

Following gene: 78224655

Centisome position: 97.13

GC content: 67.92

Gene sequence:

>1839_bases
ATGGGAGAGTGGCATCTGGACATGGGGGCGACCCCCCTTGCCGGGGGAGTTCGCTTCAGGGTATGGGCCCCTTTGGCCGA
GTCGGTGGCGGTCATCCCCTGCGGGAAAGAGGCATCGTACCCCCTCTCCCGGGCCGGCGAATACTTCCAGGGGACCATCG
CCGGCATGGCCCCGGGAGACCGTTACTGGTACCTGCTTGACGGCACTCTCCGGCGTCCCGACCCCGCGTCACGGAGCCAG
CCCGACGGGGTCCACGGTCCATCCCAAGTGGTGGCTCCGGTCTCCTTCTGCTGGACCGATGGCCAGTGGCGGGGGCTGAA
GCTTGAAGAACTGATCACCTACGAGCTCCACGTGGGGACCTTCACCCCCGTGGGTACCTTCACGGCGGCAATCAGGCGCC
TCGATTACCTGGCGGAGCTGGGAGTGACCGCGGTGGAGCTCATGCCGGTGGCGGAGTTTCCCGGCACCCGCAACTGGGGG
TATGACGGGGTTTTCCCCTTTGCCCCGCACCACGCCTACGGAGGGGCGAACGGGCTCAAGGCCCTGGTGGACGCCTGCCA
CCGGCGGGGGCTCGCCGTGATCCTCGACGTGGTCTACAACCACCTCGGCCCCGAGGGGAACTATCTCCACCCCTTTGCCC
CCTACTTCACCGACCGCTACCGCACGCCGTGGGGAGATGCCGTCAACTTCGACGGACCCGACAGCAACGGGGTGCGCCAC
TACGTAATCGCCAACGCCCTCCACTGGGTAAGCGAGTACCACGTGGACGGCCTGCGCCTCGACGCCATCCACGGCATCTA
CGACTTCAGCGCCCGCCACATCCTTCTGGACATCAGCCAGGCGGTCGATTCCCTGGCCCGGCAGCTGGGGAGGAAGGTCC
ACGTCATCGCCGAGAGCGACCTGAACGACGTGCGGACCATCACGCCGCCGGAATGGGGGGGACACGGCTGCCACGCCCAG
TGGTGCGACGATTTCCACCACGGGCTCATGACGTACCTCACCGGGGAACGTCAGGGGTATTACGCGGATTTCGGCCTCTT
CGGCCATCTGGAGAAGGCTTTTCGGGAAGGGTTCGTTTACTCCGGCCAATACTCCCGCCACCGCCGGCGCCGCCACGGCA
GCCCGTCGGCGGCCCGTCCACCCCGGCAGCTGGTGGTCTTCTCCCAGAACCACGACCAGGTGGGAAACCGGCTACGGGGG
GACCGCCCCTCGCTCCGCCTCTCCCTGGAGCAGCTCAAGCTGGCCGCCGGCGCGGTCATCCTCTCCCCCTACCTCCCCCT
TCTCTTCATGGGGGAGGAGTACAGCGAAACCGCACCCTTTCCCTACTTCACGAGCCACGGCGACCCGGAGCTGGCGGAGG
CGGTGCGGCAGGGGCGCCGGGAAGAGTTCGCCTCCTTCGGCTGGGAGGCCGAGGTGCCTGACCCCCAGGCCGAAGAGACC
TTCCTCGCCGCCAGAATCGACCCGGAGCGCCGTCACCAGGGGTGGCACCGCTCCCTGTACGCCTTTTACCGGGAAGTGAT
CCGGCTCCGCCGGGAGCTTCCGCACCTGCGCCACCCCGAGCGGGAGGAGGTGGCGGTGGTCGGGCTGGAAGCGGACCGGG
TCATCGCCCTCCGCTATCGCCATGGCGGGAGAGAACTCATCACCCTCTTTGCCCTGGGGAAAACTCCCCGCACCGTCCGG
CTCCCCTTCAGACACGGCGAATGCCGCAAGCTCCTCGATTCGGCCGGGAAGAGCTGGGGCGGTCCGGGGAGCATCGCGCC
GGAACGAATCGGCGCTGACGGCGATGGCGCAGGCCTCACCCTCGCGCCCTTCAGCGTCACGCTCTACGGCACAGGGTAA

Upstream 100 bases:

>100_bases
AGCAGGCGGGGGCCACGGAGTCGGCCCTCTTACCCTGCCGGAAAACGGCCGCGCACCGGCGCAGCCATAACCCGGCTGAC
GGCACAGGGAAGGTGCAGAC

Downstream 100 bases:

>100_bases
GAGCTACGCCGCCTCACGGAGTCACCCATGAACCAGACGAACCTTGCCAAGGCACGGATACCGACGGCAACCTACCGCCT
CCAGTTCAACGAGGGGTTCC

Product: malto-oligosyltrehalose trehalohydrolase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Glycosyltrehalose trehalohydrolase; GTHase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MGEWHLDMGATPLAGGVRFRVWAPLAESVAVIPCGKEASYPLSRAGEYFQGTIAGMAPGDRYWYLLDGTLRRPDPASRSQ
PDGVHGPSQVVAPVSFCWTDGQWRGLKLEELITYELHVGTFTPVGTFTAAIRRLDYLAELGVTAVELMPVAEFPGTRNWG
YDGVFPFAPHHAYGGANGLKALVDACHRRGLAVILDVVYNHLGPEGNYLHPFAPYFTDRYRTPWGDAVNFDGPDSNGVRH
YVIANALHWVSEYHVDGLRLDAIHGIYDFSARHILLDISQAVDSLARQLGRKVHVIAESDLNDVRTITPPEWGGHGCHAQ
WCDDFHHGLMTYLTGERQGYYADFGLFGHLEKAFREGFVYSGQYSRHRRRRHGSPSAARPPRQLVVFSQNHDQVGNRLRG
DRPSLRLSLEQLKLAAGAVILSPYLPLLFMGEEYSETAPFPYFTSHGDPELAEAVRQGRREEFASFGWEAEVPDPQAEET
FLAARIDPERRHQGWHRSLYAFYREVIRLRRELPHLRHPEREEVAVVGLEADRVIALRYRHGGRELITLFALGKTPRTVR
LPFRHGECRKLLDSAGKSWGGPGSIAPERIGADGDGAGLTLAPFSVTLYGTG

Sequences:

>Translated_612_residues
MGEWHLDMGATPLAGGVRFRVWAPLAESVAVIPCGKEASYPLSRAGEYFQGTIAGMAPGDRYWYLLDGTLRRPDPASRSQ
PDGVHGPSQVVAPVSFCWTDGQWRGLKLEELITYELHVGTFTPVGTFTAAIRRLDYLAELGVTAVELMPVAEFPGTRNWG
YDGVFPFAPHHAYGGANGLKALVDACHRRGLAVILDVVYNHLGPEGNYLHPFAPYFTDRYRTPWGDAVNFDGPDSNGVRH
YVIANALHWVSEYHVDGLRLDAIHGIYDFSARHILLDISQAVDSLARQLGRKVHVIAESDLNDVRTITPPEWGGHGCHAQ
WCDDFHHGLMTYLTGERQGYYADFGLFGHLEKAFREGFVYSGQYSRHRRRRHGSPSAARPPRQLVVFSQNHDQVGNRLRG
DRPSLRLSLEQLKLAAGAVILSPYLPLLFMGEEYSETAPFPYFTSHGDPELAEAVRQGRREEFASFGWEAEVPDPQAEET
FLAARIDPERRHQGWHRSLYAFYREVIRLRRELPHLRHPEREEVAVVGLEADRVIALRYRHGGRELITLFALGKTPRTVR
LPFRHGECRKLLDSAGKSWGGPGSIAPERIGADGDGAGLTLAPFSVTLYGTG
>Mature_611_residues
GEWHLDMGATPLAGGVRFRVWAPLAESVAVIPCGKEASYPLSRAGEYFQGTIAGMAPGDRYWYLLDGTLRRPDPASRSQP
DGVHGPSQVVAPVSFCWTDGQWRGLKLEELITYELHVGTFTPVGTFTAAIRRLDYLAELGVTAVELMPVAEFPGTRNWGY
DGVFPFAPHHAYGGANGLKALVDACHRRGLAVILDVVYNHLGPEGNYLHPFAPYFTDRYRTPWGDAVNFDGPDSNGVRHY
VIANALHWVSEYHVDGLRLDAIHGIYDFSARHILLDISQAVDSLARQLGRKVHVIAESDLNDVRTITPPEWGGHGCHAQW
CDDFHHGLMTYLTGERQGYYADFGLFGHLEKAFREGFVYSGQYSRHRRRRHGSPSAARPPRQLVVFSQNHDQVGNRLRGD
RPSLRLSLEQLKLAAGAVILSPYLPLLFMGEEYSETAPFPYFTSHGDPELAEAVRQGRREEFASFGWEAEVPDPQAEETF
LAARIDPERRHQGWHRSLYAFYREVIRLRRELPHLRHPEREEVAVVGLEADRVIALRYRHGGRELITLFALGKTPRTVRL
PFRHGECRKLLDSAGKSWGGPGSIAPERIGADGDGAGLTLAPFSVTLYGTG

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789839, Length=539, Percent_Identity=23.9332096474954, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI2367229, Length=329, Percent_Identity=27.6595744680851, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 68344; Mature: 68213

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEWHLDMGATPLAGGVRFRVWAPLAESVAVIPCGKEASYPLSRAGEYFQGTIAGMAPGD
CCCEECCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHCCHHHEEEEECCCCCC
RYWYLLDGTLRRPDPASRSQPDGVHGPSQVVAPVSFCWTDGQWRGLKLEELITYELHVGT
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCHHHEEEEEEECCC
FTPVGTFTAAIRRLDYLAELGVTAVELMPVAEFPGTRNWGYDGVFPFAPHHAYGGANGLK
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALVDACHRRGLAVILDVVYNHLGPEGNYLHPFAPYFTDRYRTPWGDAVNFDGPDSNGVRH
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEE
YVIANALHWVSEYHVDGLRLDAIHGIYDFSARHILLDISQAVDSLARQLGRKVHVIAESD
HHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCEEEEEECCC
LNDVRTITPPEWGGHGCHAQWCDDFHHGLMTYLTGERQGYYADFGLFGHLEKAFREGFVY
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCEE
SGQYSRHRRRRHGSPSAARPPRQLVVFSQNHDQVGNRLRGDRPSLRLSLEQLKLAAGAVI
CCCHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHEEEHHHHHHHHCHHH
LSPYLPLLFMGEEYSETAPFPYFTSHGDPELAEAVRQGRREEFASFGWEAEVPDPQAEET
HCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
FLAARIDPERRHQGWHRSLYAFYREVIRLRRELPHLRHPEREEVAVVGLEADRVIALRYR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEE
HGGRELITLFALGKTPRTVRLPFRHGECRKLLDSAGKSWGGPGSIAPERIGADGDGAGLT
CCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEE
LAPFSVTLYGTG
EECEEEEEEECC
>Mature Secondary Structure 
GEWHLDMGATPLAGGVRFRVWAPLAESVAVIPCGKEASYPLSRAGEYFQGTIAGMAPGD
CCEECCCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCHHHCCHHHEEEEECCCCCC
RYWYLLDGTLRRPDPASRSQPDGVHGPSQVVAPVSFCWTDGQWRGLKLEELITYELHVGT
EEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCHHHEEEEEEECCC
FTPVGTFTAAIRRLDYLAELGVTAVELMPVAEFPGTRNWGYDGVFPFAPHHAYGGANGLK
CCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ALVDACHRRGLAVILDVVYNHLGPEGNYLHPFAPYFTDRYRTPWGDAVNFDGPDSNGVRH
HHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCEEE
YVIANALHWVSEYHVDGLRLDAIHGIYDFSARHILLDISQAVDSLARQLGRKVHVIAESD
HHHHHHHHHHHHHCCCCEEEEHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCEEEEEECCC
LNDVRTITPPEWGGHGCHAQWCDDFHHGLMTYLTGERQGYYADFGLFGHLEKAFREGFVY
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCEE
SGQYSRHRRRRHGSPSAARPPRQLVVFSQNHDQVGNRLRGDRPSLRLSLEQLKLAAGAVI
CCCHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCHHEEEHHHHHHHHCHHH
LSPYLPLLFMGEEYSETAPFPYFTSHGDPELAEAVRQGRREEFASFGWEAEVPDPQAEET
HCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
FLAARIDPERRHQGWHRSLYAFYREVIRLRRELPHLRHPEREEVAVVGLEADRVIALRYR
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEE
HGGRELITLFALGKTPRTVRLPFRHGECRKLLDSAGKSWGGPGSIAPERIGADGDGAGLT
CCCCEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCEE
LAPFSVTLYGTG
EECEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8987868; 10926520 [H]