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The map label for this gene is hemD [H]

Identifier: 78224426

GI number: 78224426

Start: 3640496

End: 3642037

Strand: Direct

Name: hemD [H]

Synonym: Gmet_3235

Alternate gene names: 78224426

Gene position: 3640496-3642037 (Clockwise)

Preceding gene: 78224425

Following gene: 78224427

Centisome position: 91.07

GC content: 66.73

Gene sequence:

>1542_bases
ATGCCCGATAAATCCCTCAAAAAACCGTTCGTCCACCTCATCGGTGCCGGCCCCGGCGACCCGGGGCTCATCACCGTCAA
GGGTCGGGAGTCCCTGGGAAGAGCCGACGTGGTCATCTACGACTACCTGGCCAATGACGACCTCCTCCGCTTCGCACGGC
CCGACGCCGAGCTGATCTACGCCGGCAAGATCGGCGGGCACCACAACCGCGAGCAGTGGCAGATCAACGCGCTCCTGGTG
GAAAAGGCCCTGGCGGGGAATGTGGTGGCACGACTCAAGGGGGGTGACTCCTTTGTCTTCGGCCGGGGGGGTGAGGAGTG
CGAGGCCCTTGTGGCGGCGGGAATCCCCTTTGAGATCGTCCCCGGCGTCACGGCCGGGATCGGCGTCACTGCCTACGCCG
GCATCCCCCTCACTCACCGGGGGGTGACCACTTCCGTCACCTTCGTCACCGGCCATGAGGGGCACGACAAGGAGGTCTCC
CAGATCGACTGGGAGGGTCTCTCCCTGGGGAGCGGCACCGTAGTCTTCTACATGGGGATCAAGAACCTCCCCCAGATAAC
GGCAAACCTCATGGCCCACGGCAGGCCGCCGGAGACCCCGGTCGCCCTCATCCGCTGGGGGACTCGGCCCGAGCAGGAAG
TACTCACCGGCACCCTGGCCGACATCGCCGAGCAGGCACGGCGGACCGGCTTCCGGGCCCCGGCCATCACCGTGGTGGGA
GAGGTGGTGCGCCTGCGGGAGAAGCTCCGCTGGTTCGACAACCGTCCCCTCTTCGGCACAAGCATCCTCGTTACCCGTGC
CGCGGACCAGGCAGGCGAGTTCGGTGCCATGCTCGGGGCACTGGGGGCCCGGGTCCTGGAGTGCCCCACCATTGCCATTG
TCCCCCCCGAAAGCTGGGACAACCTCGACGCAGCCATCGCCTCTCTGGATCGGTTCCACTGGGCCGTCTTCACGTCGGTC
AACGCGGTCCGTTTCTTCTTTGAGCGACTCGCCGCAGCCGGAAAGGACTCCCGCGCCCTGGGGAACTGCCGCGTCTGCGC
CGTGGGGCCCAAGACGGCAGCAGCCCTGGCCCCCTTCGGCATCCGACCGGACCTCATTCCGGCCGACTACAAGGGTGAAG
GGGTGGTGGAGGTGTTCCGCTCGGAGGAGATTGCAGGGAAACGGATCATCTTCCCCAAGGGAGACCGGGCCAGGGACGTG
ATCCCCCAGGGCCTCGCGGAGCTGGGGGGAGAGGTGACCGCGCCGGTCGCCTACCGCAACGTGACGCCCGACTCCCTTCC
CGCCGGCGTCATCGCCGAGCTGGAAGAACGGCGCATCACCTGCGTCACCTTCACCTCGTCATCCACCGTGGAGAACCTGG
CCGCCATCCTCGGGGAGAACCGCTTCCTCCACCTCCTGAAGGGGGTGACGATCGCCGCCATCGGCCCAATCACCGCCAGA
ACCTGCCGGGAACTGGGGCTTGAGGTTCACGTGGAGCCCTCGAAGTACACCCTCGCTGCCATGACGGACGCCCTCGTCGA
CTTTTTTTCACGGTCGAAGTAG

Upstream 100 bases:

>100_bases
GGCGGAGAAGCTCCTGGCCGACGGCGCCCACGAGATCCTCGCTGAGGTCTACCAGCGGGAAGTGGCGCGGGAGAAGGAAA
TCCCGGTCTAGTCGCACCCC

Downstream 100 bases:

>100_bases
ATACGCCTCTCCTTCACCCCTCGCCCCTGCAACCGGAGTGTGGAGCGCCCCGATGCTCACCGACACCCACTGCCACCTGG
ATGACCCGACCCTCAGCTCG

Product: uroporphyrinogen-III synthase/uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MPDKSLKKPFVHLIGAGPGDPGLITVKGRESLGRADVVIYDYLANDDLLRFARPDAELIYAGKIGGHHNREQWQINALLV
EKALAGNVVARLKGGDSFVFGRGGEECEALVAAGIPFEIVPGVTAGIGVTAYAGIPLTHRGVTTSVTFVTGHEGHDKEVS
QIDWEGLSLGSGTVVFYMGIKNLPQITANLMAHGRPPETPVALIRWGTRPEQEVLTGTLADIAEQARRTGFRAPAITVVG
EVVRLREKLRWFDNRPLFGTSILVTRAADQAGEFGAMLGALGARVLECPTIAIVPPESWDNLDAAIASLDRFHWAVFTSV
NAVRFFFERLAAAGKDSRALGNCRVCAVGPKTAAALAPFGIRPDLIPADYKGEGVVEVFRSEEIAGKRIIFPKGDRARDV
IPQGLAELGGEVTAPVAYRNVTPDSLPAGVIAELEERRITCVTFTSSSTVENLAAILGENRFLHLLKGVTIAAIGPITAR
TCRELGLEVHVEPSKYTLAAMTDALVDFFSRSK

Sequences:

>Translated_513_residues
MPDKSLKKPFVHLIGAGPGDPGLITVKGRESLGRADVVIYDYLANDDLLRFARPDAELIYAGKIGGHHNREQWQINALLV
EKALAGNVVARLKGGDSFVFGRGGEECEALVAAGIPFEIVPGVTAGIGVTAYAGIPLTHRGVTTSVTFVTGHEGHDKEVS
QIDWEGLSLGSGTVVFYMGIKNLPQITANLMAHGRPPETPVALIRWGTRPEQEVLTGTLADIAEQARRTGFRAPAITVVG
EVVRLREKLRWFDNRPLFGTSILVTRAADQAGEFGAMLGALGARVLECPTIAIVPPESWDNLDAAIASLDRFHWAVFTSV
NAVRFFFERLAAAGKDSRALGNCRVCAVGPKTAAALAPFGIRPDLIPADYKGEGVVEVFRSEEIAGKRIIFPKGDRARDV
IPQGLAELGGEVTAPVAYRNVTPDSLPAGVIAELEERRITCVTFTSSSTVENLAAILGENRFLHLLKGVTIAAIGPITAR
TCRELGLEVHVEPSKYTLAAMTDALVDFFSRSK
>Mature_512_residues
PDKSLKKPFVHLIGAGPGDPGLITVKGRESLGRADVVIYDYLANDDLLRFARPDAELIYAGKIGGHHNREQWQINALLVE
KALAGNVVARLKGGDSFVFGRGGEECEALVAAGIPFEIVPGVTAGIGVTAYAGIPLTHRGVTTSVTFVTGHEGHDKEVSQ
IDWEGLSLGSGTVVFYMGIKNLPQITANLMAHGRPPETPVALIRWGTRPEQEVLTGTLADIAEQARRTGFRAPAITVVGE
VVRLREKLRWFDNRPLFGTSILVTRAADQAGEFGAMLGALGARVLECPTIAIVPPESWDNLDAAIASLDRFHWAVFTSVN
AVRFFFERLAAAGKDSRALGNCRVCAVGPKTAAALAPFGIRPDLIPADYKGEGVVEVFRSEEIAGKRIIFPKGDRARDVI
PQGLAELGGEVTAPVAYRNVTPDSLPAGVIAELEERRITCVTFTSSSTVENLAAILGENRFLHLLKGVTIAAIGPITART
CRELGLEVHVEPSKYTLAAMTDALVDFFSRSK

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=257, Percent_Identity=45.9143968871595, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=29.7872340425532, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55001; Mature: 54870

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKSLKKPFVHLIGAGPGDPGLITVKGRESLGRADVVIYDYLANDDLLRFARPDAELIY
CCCHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEE
AGKIGGHHNREQWQINALLVEKALAGNVVARLKGGDSFVFGRGGEECEALVAAGIPFEIV
EECCCCCCCCCCEEEHHHHHHHHHHCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEC
PGVTAGIGVTAYAGIPLTHRGVTTSVTFVTGHEGHDKEVSQIDWEGLSLGSGTVVFYMGI
CCCCCCCCEEEECCCCEECCCCEEEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEECC
KNLPQITANLMAHGRPPETPVALIRWGTRPEQEVLTGTLADIAEQARRTGFRAPAITVVG
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EVVRLREKLRWFDNRPLFGTSILVTRAADQAGEFGAMLGALGARVLECPTIAIVPPESWD
HHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
NLDAAIASLDRFHWAVFTSVNAVRFFFERLAAAGKDSRALGNCRVCAVGPKTAAALAPFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCC
IRPDLIPADYKGEGVVEVFRSEEIAGKRIIFPKGDRARDVIPQGLAELGGEVTAPVAYRN
CCCCCCCCCCCCCCHHEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEECCEEECC
VTPDSLPAGVIAELEERRITCVTFTSSSTVENLAAILGENRFLHLLKGVTIAAIGPITAR
CCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCHHHH
TCRELGLEVHVEPSKYTLAAMTDALVDFFSRSK
HHHHCCCEEEECCCCEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDKSLKKPFVHLIGAGPGDPGLITVKGRESLGRADVVIYDYLANDDLLRFARPDAELIY
CCHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEE
AGKIGGHHNREQWQINALLVEKALAGNVVARLKGGDSFVFGRGGEECEALVAAGIPFEIV
EECCCCCCCCCCEEEHHHHHHHHHHCCEEEEEECCCEEEECCCCHHHHHHHHCCCCEEEC
PGVTAGIGVTAYAGIPLTHRGVTTSVTFVTGHEGHDKEVSQIDWEGLSLGSGTVVFYMGI
CCCCCCCCEEEECCCCEECCCCEEEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEECC
KNLPQITANLMAHGRPPETPVALIRWGTRPEQEVLTGTLADIAEQARRTGFRAPAITVVG
CCCHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EVVRLREKLRWFDNRPLFGTSILVTRAADQAGEFGAMLGALGARVLECPTIAIVPPESWD
HHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
NLDAAIASLDRFHWAVFTSVNAVRFFFERLAAAGKDSRALGNCRVCAVGPKTAAALAPFG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCC
IRPDLIPADYKGEGVVEVFRSEEIAGKRIIFPKGDRARDVIPQGLAELGGEVTAPVAYRN
CCCCCCCCCCCCCCHHEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEECCEEECC
VTPDSLPAGVIAELEERRITCVTFTSSSTVENLAAILGENRFLHLLKGVTIAAIGPITAR
CCCCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHCCCEEEEECCHHHH
TCRELGLEVHVEPSKYTLAAMTDALVDFFSRSK
HHHHCCCEEEECCCCEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]