Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 78224345

GI number: 78224345

Start: 3550082

End: 3550456

Strand: Reverse

Name: gcvH [H]

Synonym: Gmet_3154

Alternate gene names: 78224345

Gene position: 3550456-3550082 (Counterclockwise)

Preceding gene: 78224346

Following gene: 78224344

Centisome position: 88.82

GC content: 60.53

Gene sequence:

>375_bases
ATGGAAATCTATTTCACCAAGGAGCACGAGTGGGTCAAGGTCAAGGAAGGAGTTGCGTTGGTGGGGATCACCGAGCATGC
GGCCCACCAGTTGGGGGATGTGACTTTCGTGGAGCTTCCGAAAGTGGGTAAGGTCGTGAAGCAGTTCGAGGTCCTGGCGG
CCATCGAGTCGGTGAAGGCGGCCAGCGACATCTACGCCCCGGTTTCCGGCACGGTGACCCAGGTGAACGAGGCCCTTGAA
GAGCGCCCCGAGATCGTCAACGAGGCGGCCGAGGACGCCGGCTGGATGGCAATTATGGACATGGAGGATCCGGCCGAGCT
CCAGAAGCTCATGACCCGCGACCAGTATGACGAGTACGTGAGGAGCCTCGGATGA

Upstream 100 bases:

>100_bases
CGACGGTGGTGGAGCTTCCCTTCTACCGGGGCGGGTCGCTTCGTTCATAAGAAATTTTTCGCCGGATACGGATTCTGTTA
TCAAAATCAAGGAGGGATAC

Downstream 100 bases:

>100_bases
GCACTGCCAGCTACTGCCCCAACACGCCAGAGGAGGTACGGGAGATGCTGGCCGCCATGGGCGCGGCGAGCATCGACGAC
CTGTTCCAGCCGATTCCGCC

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MEIYFTKEHEWVKVKEGVALVGITEHAAHQLGDVTFVELPKVGKVVKQFEVLAAIESVKAASDIYAPVSGTVTQVNEALE
ERPEIVNEAAEDAGWMAIMDMEDPAELQKLMTRDQYDEYVRSLG

Sequences:

>Translated_124_residues
MEIYFTKEHEWVKVKEGVALVGITEHAAHQLGDVTFVELPKVGKVVKQFEVLAAIESVKAASDIYAPVSGTVTQVNEALE
ERPEIVNEAAEDAGWMAIMDMEDPAELQKLMTRDQYDEYVRSLG
>Mature_124_residues
MEIYFTKEHEWVKVKEGVALVGITEHAAHQLGDVTFVELPKVGKVVKQFEVLAAIESVKAASDIYAPVSGTVTQVNEALE
ERPEIVNEAAEDAGWMAIMDMEDPAELQKLMTRDQYDEYVRSLG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=43.6974789915966, Blast_Score=122, Evalue=6e-29,
Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=43.6974789915966, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1789271, Length=117, Percent_Identity=47.8632478632479, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=117, Percent_Identity=47.8632478632479, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=44.3478260869565, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=43.3333333333333, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=124, Percent_Identity=45.1612903225806, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13824; Mature: 13824

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIYFTKEHEWVKVKEGVALVGITEHAAHQLGDVTFVELPKVGKVVKQFEVLAAIESVKA
CEEEECCCCCEEEECCCEEEEEEHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGTVTQVNEALEERPEIVNEAAEDAGWMAIMDMEDPAELQKLMTRDQYDEYV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RSLG
HHCC
>Mature Secondary Structure
MEIYFTKEHEWVKVKEGVALVGITEHAAHQLGDVTFVELPKVGKVVKQFEVLAAIESVKA
CEEEECCCCCEEEECCCEEEEEEHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHH
ASDIYAPVSGTVTQVNEALEERPEIVNEAAEDAGWMAIMDMEDPAELQKLMTRDQYDEYV
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
RSLG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA