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The map label for this gene is lipA

Identifier: 78224341

GI number: 78224341

Start: 3545635

End: 3546486

Strand: Reverse

Name: lipA

Synonym: Gmet_3150

Alternate gene names: 78224341

Gene position: 3546486-3545635 (Counterclockwise)

Preceding gene: 78224342

Following gene: 78224340

Centisome position: 88.72

GC content: 67.14

Gene sequence:

>852_bases
ATGACCATCGTCCGCAAGCCCCAGTGGCTGCAAAAGAAGATCAACCCCGCGGCCCACGCCGGCATGGAGGGGCTTCTGGG
GGAGCTGCGCCTTCACACGGTCTGTCAGGAGGCCCGCTGCCCCAACATTACCGAGTGCTTCCGGGAGCGACAGGCGACCT
TCCTCATCCTGGGGGCCGCATGCACCCGCCTCTGCTCCTTCTGCAACGTGACGAAACAGACACCCATCCCTCCCGACCCG
GGCGAGCCGGACCGGGTTGCGGAGGCGATCCGCCGCCTTGGTCTCTCCCACGTGGTCATCACGAGCCCCACCCGGGACGA
CCTCCCCGACGGCGGTGCCGGCCACTATGCCGAAACCGTCGCCGCCATCCGGAGTGCGTCTCCCGCCACCACGGTGGAGC
TCCTCATTCCTGATTACCTCGGCAACCGCGAGAGCCTCGCCCGGGTCGTCGCCTCGGCGCCGGCCATCATCGGCCACAAT
GTCGAGACGGTGCCGCGCCTCTACCAAATCCGGGCCGGGGCCGACTACGGCCGCTCCCTTGGAGTGCTCCGGACCCTGCG
TGAACTGGACCCTGTCGTGCGAAGCAAGTCGGGAATCATGCTCGGCCTCGGCGAGGCGGAGGAGGAGGTGCTGGCGGTTT
TCGCCGACCTGCGGTCGGTGGGGTGTTCCTACCTCAGCATCGGCCAGTACCTGGCCCCCAGCAAGAGCCACCATCCCGTC
AGGGAATTCATTCCTCCCGAATGTTTCGAAAGATACCGGGCAGCGGCCCTGGCCACGGGGTTTGCCCACGTGGAGAGCGG
CCCCTACGTGAGGAGTTCCTACCACGCGGCCCGTTACGACGGGCAACTGTGA

Upstream 100 bases:

>100_bases
TCGTTCTTGCGGTCCTCACCGCCGAGGAAGAGGCGACCGCGGCCCGCCTCGGCGCGGAGCGTTACGGGAAAGCTGCCTGG
AACCTGCACGGAGAAACGCC

Downstream 100 bases:

>100_bases
CCCTCGGCACGAAACGTGCTGATTATTCGCCAGAACACCTCGGGGAGGTTGTGACGGATATTGGCGCGTGGAGGTCGACA
GGTGGTGTGCGAATGGTGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTIVRKPQWLQKKINPAAHAGMEGLLGELRLHTVCQEARCPNITECFRERQATFLILGAACTRLCSFCNVTKQTPIPPDP
GEPDRVAEAIRRLGLSHVVITSPTRDDLPDGGAGHYAETVAAIRSASPATTVELLIPDYLGNRESLARVVASAPAIIGHN
VETVPRLYQIRAGADYGRSLGVLRTLRELDPVVRSKSGIMLGLGEAEEEVLAVFADLRSVGCSYLSIGQYLAPSKSHHPV
REFIPPECFERYRAAALATGFAHVESGPYVRSSYHAARYDGQL

Sequences:

>Translated_283_residues
MTIVRKPQWLQKKINPAAHAGMEGLLGELRLHTVCQEARCPNITECFRERQATFLILGAACTRLCSFCNVTKQTPIPPDP
GEPDRVAEAIRRLGLSHVVITSPTRDDLPDGGAGHYAETVAAIRSASPATTVELLIPDYLGNRESLARVVASAPAIIGHN
VETVPRLYQIRAGADYGRSLGVLRTLRELDPVVRSKSGIMLGLGEAEEEVLAVFADLRSVGCSYLSIGQYLAPSKSHHPV
REFIPPECFERYRAAALATGFAHVESGPYVRSSYHAARYDGQL
>Mature_282_residues
TIVRKPQWLQKKINPAAHAGMEGLLGELRLHTVCQEARCPNITECFRERQATFLILGAACTRLCSFCNVTKQTPIPPDPG
EPDRVAEAIRRLGLSHVVITSPTRDDLPDGGAGHYAETVAAIRSASPATTVELLIPDYLGNRESLARVVASAPAIIGHNV
ETVPRLYQIRAGADYGRSLGVLRTLRELDPVVRSKSGIMLGLGEAEEEVLAVFADLRSVGCSYLSIGQYLAPSKSHHPVR
EFIPPECFERYRAAALATGFAHVESGPYVRSSYHAARYDGQL

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=42.1052631578947, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=42.2764227642276, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1786846, Length=279, Percent_Identity=47.3118279569892, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI32564533, Length=288, Percent_Identity=40.9722222222222, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=280, Percent_Identity=43.2142857142857, Blast_Score=226, Evalue=3e-60,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=41.2587412587413, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_GEOMG (Q39QW1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386088.1
- ProteinModelPortal:   Q39QW1
- STRING:   Q39QW1
- GeneID:   3741119
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3150
- NMPDR:   fig|269799.3.peg.2383
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   ARCPNIT
- PhylomeDB:   Q39QW1
- ProtClustDB:   PRK05481
- BioCyc:   GMET269799:GMET_3150-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 30826; Mature: 30694

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVRKPQWLQKKINPAAHAGMEGLLGELRLHTVCQEARCPNITECFRERQATFLILGAA
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEHHHH
CTRLCSFCNVTKQTPIPPDPGEPDRVAEAIRRLGLSHVVITSPTRDDLPDGGAGHYAETV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHH
AAIRSASPATTVELLIPDYLGNRESLARVVASAPAIIGHNVETVPRLYQIRAGADYGRSL
HHHHCCCCCCEEEEECCHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCHHHH
GVLRTLRELDPVVRSKSGIMLGLGEAEEEVLAVFADLRSVGCSYLSIGQYLAPSKSHHPV
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCH
REFIPPECFERYRAAALATGFAHVESGPYVRSSYHAARYDGQL
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCC
>Mature Secondary Structure 
TIVRKPQWLQKKINPAAHAGMEGLLGELRLHTVCQEARCPNITECFRERQATFLILGAA
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEHHHH
CTRLCSFCNVTKQTPIPPDPGEPDRVAEAIRRLGLSHVVITSPTRDDLPDGGAGHYAETV
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHH
AAIRSASPATTVELLIPDYLGNRESLARVVASAPAIIGHNVETVPRLYQIRAGADYGRSL
HHHHCCCCCCEEEEECCHHCCCHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCHHHH
GVLRTLRELDPVVRSKSGIMLGLGEAEEEVLAVFADLRSVGCSYLSIGQYLAPSKSHHPV
HHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCH
REFIPPECFERYRAAALATGFAHVESGPYVRSSYHAARYDGQL
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA