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The map label for this gene is truD

Identifier: 78224226

GI number: 78224226

Start: 3418230

End: 3419444

Strand: Reverse

Name: truD

Synonym: Gmet_3033

Alternate gene names: 78224226

Gene position: 3419444-3418230 (Counterclockwise)

Preceding gene: 78224227

Following gene: 78224225

Centisome position: 85.54

GC content: 69.55

Gene sequence:

>1215_bases
GTGAGCGGCGAGAAGCGCTATCTGACCGCCGGCCTCCCCGGCACCGGAGGCACCATCAAGGAGACCGCCGACGATTTCGT
GGTGGAGGAGATTCCCCTCTACCTCCCCTGTGGCGAGGGAGAGCACGTCTACGCCCTCATCGAGAAGCGGGGGGTGACGA
CCCTGGACGCCATCCGCCGCCTGGCCCGGGCACTGAAGCTCTCCGAGCGGGACGTGGGGTATGCGGGCATGAAGGATGCC
CGGGGGGTGACCCGCCAGACCGTGTCGCTCCCCCGGATGAAGCCCGAGGAGGTGCTCGCCCTGGAGCTTCCCGGCATCCG
CATTCTCTCCGCGGAACGGCACCGGAACAAGCTGAAGCTGGGGCACCTGGCGGGAAACCGGTTTCGGATCCGAGTCAGGG
GGGTGGTGTCCGACGCCGTGGCCCGGGCCGAGGCGATCCTGGCGGTGCTGGAACGGCGCGGCGTCCCCAACCGCTTCGGG
GAGCAGCGCTACGGTGCCCAGGGGAACAGCCATCTCATCGGCCGGGCCATGCTGGCCGGCGACTGGTGCGCTGCGGTGGA
TCTCCTCATGGGCGATCCGGCAAAGGTTACGGGGGAGGCATGGCGGAGCGCCATCGAGGCGTACCAACGAGGGGAACTGG
AGGAGAGCCAGCGCCTCTTCCCGGGCCACTGCCGCACCGAGCGGGACGTCATCCAGCGTCTCGTGAAGCGCCCCGACGAC
TTCGAGGGGGCCTTCCGCGCGGTCAATCCCCGGCTCAGGAAGCTCTACCTCTCCGCCTGCCAGTCGGCTCTTTTCGACCA
CGTGGTGGAGGCCCGGCTCGACTCCCTCGACACCGTCCAGGAGGGTGATCTGGCCTGGAAGCACGCCAACGGCGCCTGTT
TCCTGGTAACGGACCCCGCGGCCGAAGCGCCACGGGCGGAGCACTTCGAGATCTCCCCCACCGGACCCCTCTTCGGCTGC
CGGATGATGATGCCCGAGGGTGAAGAGGGGGCGCTGGAACGGTCGCTTCTGGCGGCCGAAGGGGTGGAGCCGGCCAGCTT
CGACCTCCCCGGCGGGCTCCGGATGGAGGGGGAGCGGCGACCCCTTCGGGTTCCCCTGGGGGATCCGCGGGCTTCCGCGG
ACGCTGACGGGCTGGTGCTGGAGTTCTCGCTTCCCAAGGGGAGCTACGCCACGGCAGTCCTGCGGGAGGTCATGAAGGGG
GGTAGGCCGGATTGA

Upstream 100 bases:

>100_bases
AGTTGGAAGGGTACCATCTCTCCAAGTACATGATGAAGTTCATGGCCGAGGGGAAGCGGATCTACTTCGTCCACTACCGG
AAAACGGCAGAGGGGGCAGC

Downstream 100 bases:

>100_bases
ATTACGCCCGCGGCCTGGAGAAATCGCGGTAAAAGCCGATACAATGTAAAACAGCCGCAATGCAGCCGATTCCCCATGGG
GAGGTGCTCTATGGAGCATG

Product: tRNA pseudouridine synthase D, TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSGEKRYLTAGLPGTGGTIKETADDFVVEEIPLYLPCGEGEHVYALIEKRGVTTLDAIRRLARALKLSERDVGYAGMKDA
RGVTRQTVSLPRMKPEEVLALELPGIRILSAERHRNKLKLGHLAGNRFRIRVRGVVSDAVARAEAILAVLERRGVPNRFG
EQRYGAQGNSHLIGRAMLAGDWCAAVDLLMGDPAKVTGEAWRSAIEAYQRGELEESQRLFPGHCRTERDVIQRLVKRPDD
FEGAFRAVNPRLRKLYLSACQSALFDHVVEARLDSLDTVQEGDLAWKHANGACFLVTDPAAEAPRAEHFEISPTGPLFGC
RMMMPEGEEGALERSLLAAEGVEPASFDLPGGLRMEGERRPLRVPLGDPRASADADGLVLEFSLPKGSYATAVLREVMKG
GRPD

Sequences:

>Translated_404_residues
MSGEKRYLTAGLPGTGGTIKETADDFVVEEIPLYLPCGEGEHVYALIEKRGVTTLDAIRRLARALKLSERDVGYAGMKDA
RGVTRQTVSLPRMKPEEVLALELPGIRILSAERHRNKLKLGHLAGNRFRIRVRGVVSDAVARAEAILAVLERRGVPNRFG
EQRYGAQGNSHLIGRAMLAGDWCAAVDLLMGDPAKVTGEAWRSAIEAYQRGELEESQRLFPGHCRTERDVIQRLVKRPDD
FEGAFRAVNPRLRKLYLSACQSALFDHVVEARLDSLDTVQEGDLAWKHANGACFLVTDPAAEAPRAEHFEISPTGPLFGC
RMMMPEGEEGALERSLLAAEGVEPASFDLPGGLRMEGERRPLRVPLGDPRASADADGLVLEFSLPKGSYATAVLREVMKG
GRPD
>Mature_403_residues
SGEKRYLTAGLPGTGGTIKETADDFVVEEIPLYLPCGEGEHVYALIEKRGVTTLDAIRRLARALKLSERDVGYAGMKDAR
GVTRQTVSLPRMKPEEVLALELPGIRILSAERHRNKLKLGHLAGNRFRIRVRGVVSDAVARAEAILAVLERRGVPNRFGE
QRYGAQGNSHLIGRAMLAGDWCAAVDLLMGDPAKVTGEAWRSAIEAYQRGELEESQRLFPGHCRTERDVIQRLVKRPDDF
EGAFRAVNPRLRKLYLSACQSALFDHVVEARLDSLDTVQEGDLAWKHANGACFLVTDPAAEAPRAEHFEISPTGPLFGCR
MMMPEGEEGALERSLLAAEGVEPASFDLPGGLRMEGERRPLRVPLGDPRASADADGLVLEFSLPKGSYATAVLREVMKGG
RPD

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Homo sapiens, GI148762985, Length=407, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148762961, Length=407, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI148762948, Length=407, Percent_Identity=24.3243243243243, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI50727002, Length=406, Percent_Identity=24.1379310344828, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1789102, Length=167, Percent_Identity=38.9221556886228, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17557232, Length=389, Percent_Identity=27.5064267352185, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324817, Length=251, Percent_Identity=24.7011952191235, Blast_Score=83, Evalue=8e-17,
Organism=Drosophila melanogaster, GI21355799, Length=241, Percent_Identity=29.4605809128631, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_GEOMG (Q39R76)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385973.1
- ProteinModelPortal:   Q39R76
- SMR:   Q39R76
- STRING:   Q39R76
- GeneID:   3741229
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3033
- NMPDR:   fig|269799.3.peg.3083
- eggNOG:   COG0585
- HOGENOM:   HBG541221
- OMA:   WAGTKDK
- PhylomeDB:   Q39R76
- ProtClustDB:   CLSK827897
- BioCyc:   GMET269799:GMET_3033-MONOMER
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 44283; Mature: 44152

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 79-79

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEKRYLTAGLPGTGGTIKETADDFVVEEIPLYLPCGEGEHVYALIEKRGVTTLDAIRR
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHHHH
LARALKLSERDVGYAGMKDARGVTRQTVSLPRMKPEEVLALELPGIRILSAERHRNKLKL
HHHHHHCCCCCCCCCCCHHHCCCHHHHHCCCCCCCCCEEEEECCCEEEEECHHHCCCEEE
GHLAGNRFRIRVRGVVSDAVARAEAILAVLERRGVPNRFGEQRYGAQGNSHLIGRAMLAG
EEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEHHHHHHC
DWCAAVDLLMGDPAKVTGEAWRSAIEAYQRGELEESQRLFPGHCRTERDVIQRLVKRPDD
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCC
FEGAFRAVNPRLRKLYLSACQSALFDHVVEARLDSLDTVQEGDLAWKHANGACFLVTDPA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCC
AEAPRAEHFEISPTGPLFGCRMMMPEGEEGALERSLLAAEGVEPASFDLPGGLRMEGERR
CCCCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
PLRVPLGDPRASADADGLVLEFSLPKGSYATAVLREVMKGGRPD
CEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SGEKRYLTAGLPGTGGTIKETADDFVVEEIPLYLPCGEGEHVYALIEKRGVTTLDAIRR
CCCCEEEEECCCCCCCCHHHHHHHHHHHHCCEEEECCCCCEEEEEEECCCCCHHHHHHH
LARALKLSERDVGYAGMKDARGVTRQTVSLPRMKPEEVLALELPGIRILSAERHRNKLKL
HHHHHHCCCCCCCCCCCHHHCCCHHHHHCCCCCCCCCEEEEECCCEEEEECHHHCCCEEE
GHLAGNRFRIRVRGVVSDAVARAEAILAVLERRGVPNRFGEQRYGAQGNSHLIGRAMLAG
EEECCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEHHHHHHC
DWCAAVDLLMGDPAKVTGEAWRSAIEAYQRGELEESQRLFPGHCRTERDVIQRLVKRPDD
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCC
FEGAFRAVNPRLRKLYLSACQSALFDHVVEARLDSLDTVQEGDLAWKHANGACFLVTDPA
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCC
AEAPRAEHFEISPTGPLFGCRMMMPEGEEGALERSLLAAEGVEPASFDLPGGLRMEGERR
CCCCCCCCEEECCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEECCCCC
PLRVPLGDPRASADADGLVLEFSLPKGSYATAVLREVMKGGRPD
CEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA