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The map label for this gene is prfA [H]

Identifier: 78224169

GI number: 78224169

Start: 3364019

End: 3364384

Strand: Reverse

Name: prfA [H]

Synonym: Gmet_2976

Alternate gene names: 78224169

Gene position: 3364384-3364019 (Counterclockwise)

Preceding gene: 78224170

Following gene: 78224168

Centisome position: 84.16

GC content: 64.21

Gene sequence:

>366_bases
ATGGCCACCCCGACCTTTGCCGTCAGCGAGGAGAAGAACCGCTGGCTCCGGGAGAAGATGACGGAGCTGGGGGTGGCCGA
GGCCGACCTGGAAGAGAGCTTCGTCCGCTCCTCCGGCGCAGGGGGGCAGCACGTGAACAAGACCGCCACCTGCGTCCAGA
TCAAGCACCGCCCCACGGGAATCGAGGTGAAGTGCATGAAGGACCGGAGCCAGTCGGTGAACCGGTTCCTGGCCCGACGG
GAGATTCTGGTGCGGATCGAGCGGTTGCAGAAGGGAGAGAGCTTCCGGGACCCCAAGGCGGAAAAGCTGAAAAAGCAGAA
GGCGCGGCGGAAGCGGCGCGCCAGCGGGAAATACGAGAAGGAATAA

Upstream 100 bases:

>100_bases
GATCCTCGTGGCCGTGGCCGGTCCGCTCCTGGGGAGGCTCTACCTGGAACAGCGCCATCGGCGGCTCTACCACCTTGACG
ACGTGGAGGAGGCGTAGCCC

Downstream 100 bases:

>100_bases
GTCCCAAAACCATTTAATAACACAGAGCAACTGAGCAACAGAGTAAACTCGATAGGCTGAAGGTCACACCCTTGACAATC
GTTTGTTAGGCTCTTTCCGA

Product: class I peptide chain release factor

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 121; Mature: 120

Protein sequence:

>121_residues
MATPTFAVSEEKNRWLREKMTELGVAEADLEESFVRSSGAGGQHVNKTATCVQIKHRPTGIEVKCMKDRSQSVNRFLARR
EILVRIERLQKGESFRDPKAEKLKKQKARRKRRASGKYEKE

Sequences:

>Translated_121_residues
MATPTFAVSEEKNRWLREKMTELGVAEADLEESFVRSSGAGGQHVNKTATCVQIKHRPTGIEVKCMKDRSQSVNRFLARR
EILVRIERLQKGESFRDPKAEKLKKQKARRKRRASGKYEKE
>Mature_120_residues
ATPTFAVSEEKNRWLREKMTELGVAEADLEESFVRSSGAGGQHVNKTATCVQIKHRPTGIEVKCMKDRSQSVNRFLARRE
ILVRIERLQKGESFRDPKAEKLKKQKARRKRRASGKYEKE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI303304971, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=3e-13,
Organism=Homo sapiens, GI219879802, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=3e-13,
Organism=Homo sapiens, GI22748627, Length=94, Percent_Identity=38.2978723404255, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367172, Length=69, Percent_Identity=42.0289855072464, Blast_Score=63, Evalue=5e-12,
Organism=Escherichia coli, GI1787462, Length=73, Percent_Identity=47.945205479452, Blast_Score=60, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24654113, Length=91, Percent_Identity=42.8571428571429, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 13974; Mature: 13843

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPTFAVSEEKNRWLREKMTELGVAEADLEESFVRSSGAGGQHVNKTATCVQIKHRPTG
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
IEVKCMKDRSQSVNRFLARREILVRIERLQKGESFRDPKAEKLKKQKARRKRRASGKYEK
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
E
C
>Mature Secondary Structure 
ATPTFAVSEEKNRWLREKMTELGVAEADLEESFVRSSGAGGQHVNKTATCVQIKHRPTG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCC
IEVKCMKDRSQSVNRFLARREILVRIERLQKGESFRDPKAEKLKKQKARRKRRASGKYEK
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA