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The map label for this gene is dhfrIII [H]

Identifier: 78224143

GI number: 78224143

Start: 3337712

End: 3338197

Strand: Reverse

Name: dhfrIII [H]

Synonym: Gmet_2947

Alternate gene names: 78224143

Gene position: 3338197-3337712 (Counterclockwise)

Preceding gene: 78224144

Following gene: 78224142

Centisome position: 83.51

GC content: 66.05

Gene sequence:

>486_bases
ATGATCGTCACCATCATTGCGGCCATGGCCGGGAACCGGGTCATCGGGAAGGAGGGCGCCATGCCGTGGCACCTCCCCGA
CGATCTCGCCCGCTTCAAGGCCATCACCATGGGGCATCCGGTCGTCATGGGGCGAAAGACCTTCGAATCCATCGGTCGTC
CCCTGCCGGGGCGGCTGAATATCGTCCTCACCCGGCAGGCGGGGTATGCCCCCTCAGGGGTGACCGTTGCCCGGACCCTT
GCCGAGGCCTTGATCCTTGCGGGGACCGCCGGAGAAGTCTTCATCTGCGGCGGCGGTGAGGTCTACCGGGAGGCGTTGCC
GCTGGCCGACCGCATCCACCTCACCGTTATTCACCGTGCTTACGGAGGGGACACCACCTTTCCCGAGCTTCCCGCCGATT
TCGAGGAAACGTGCCGGGAGCATGGGGAGGGGAAGCCGCCTCACTCCTTCATCACCTTCGAGCGGCGGAGGGGGTCGACG
CCATGA

Upstream 100 bases:

>100_bases
CGGTGGTGACTCTGCTCGACATGCTCCTCAAGTGGCGCGGCTACCTCTCCGAGCGGAGACCCATCACCACGAGCGAGGTG
CTGGAGTTCGAGGAGCCGGC

Downstream 100 bases:

>100_bases
CCGAACGCCGCTTTTCCGTCTTTCTCGTACCTGCATCCGATGACCGCCGCTGGGCCCAGGGGGTGATCGCCGAGCTTGCC
GCCCGCTACGACACACCTCC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIVTIIAAMAGNRVIGKEGAMPWHLPDDLARFKAITMGHPVVMGRKTFESIGRPLPGRLNIVLTRQAGYAPSGVTVARTL
AEALILAGTAGEVFICGGGEVYREALPLADRIHLTVIHRAYGGDTTFPELPADFEETCREHGEGKPPHSFITFERRRGST
P

Sequences:

>Translated_161_residues
MIVTIIAAMAGNRVIGKEGAMPWHLPDDLARFKAITMGHPVVMGRKTFESIGRPLPGRLNIVLTRQAGYAPSGVTVARTL
AEALILAGTAGEVFICGGGEVYREALPLADRIHLTVIHRAYGGDTTFPELPADFEETCREHGEGKPPHSFITFERRRGST
P
>Mature_161_residues
MIVTIIAAMAGNRVIGKEGAMPWHLPDDLARFKAITMGHPVVMGRKTFESIGRPLPGRLNIVLTRQAGYAPSGVTVARTL
AEALILAGTAGEVFICGGGEVYREALPLADRIHLTVIHRAYGGDTTFPELPADFEETCREHGEGKPPHSFITFERRRGST
P

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=148, Percent_Identity=35.1351351351351, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=45.625, Blast_Score=136, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17505899, Length=151, Percent_Identity=35.0993377483444, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=137, Percent_Identity=30.6569343065693, Blast_Score=69, Evalue=5e-13,
Organism=Drosophila melanogaster, GI116008026, Length=150, Percent_Identity=36.6666666666667, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647458, Length=150, Percent_Identity=36.6666666666667, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17417; Mature: 17417

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTIIAAMAGNRVIGKEGAMPWHLPDDLARFKAITMGHPVVMGRKTFESIGRPLPGRLN
CEEEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCEE
IVLTRQAGYAPSGVTVARTLAEALILAGTAGEVFICGGGEVYREALPLADRIHLTVIHRA
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEEEE
YGGDTTFPELPADFEETCREHGEGKPPHSFITFERRRGSTP
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MIVTIIAAMAGNRVIGKEGAMPWHLPDDLARFKAITMGHPVVMGRKTFESIGRPLPGRLN
CEEEEEEEHHCCEEECCCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHCCCCCCCEE
IVLTRQAGYAPSGVTVARTLAEALILAGTAGEVFICGGGEVYREALPLADRIHLTVIHRA
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCEEEEEEEEE
YGGDTTFPELPADFEETCREHGEGKPPHSFITFERRRGSTP
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]