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The map label for this gene is thiG

Identifier: 78224130

GI number: 78224130

Start: 3320005

End: 3320787

Strand: Direct

Name: thiG

Synonym: Gmet_2934

Alternate gene names: 78224130

Gene position: 3320005-3320787 (Clockwise)

Preceding gene: 78224129

Following gene: 78224131

Centisome position: 83.05

GC content: 63.47

Gene sequence:

>783_bases
ATGTCCACTACGAACGACAAGCTGATCATCGCCGGCCGGGAATTCAGCTCCCGCCTCATGGTCGGAACCGGCAAATACGC
CAGTAACGAGCAGATGGTGGCGGCCCTGGAGGCCTCGGGAGCCGAGATCATCACCGTGGCGGTGCGGCGAGTCAACATCA
CCGACCGCTCCAAGGAGTCGCTCCTGGACTTCATCGACACCAGGAAATACACGCTTCTTCCCAATACCGCCGGCTGCTAC
ACGGCCGACGACGCGGTCCGCACCTGCCGACTCGCCCGGGAAGCGGGGATGAGCGACATGGTTAAACTGGAGGTTCTCGG
CAACGAGACGACCCTCTACCCCGACAACGAGGAGCTCCTCAAGGCGGCCAAGATCCTCGTCAAGGACGGGTTCACAGTCC
TTCCCTATACGAGCGACGACCCCATCATCTGCAAGAAGCTGGAGGACATCGGCTGTGCCGCAGTCATGCCGCTTGGCGCA
CCCATCGGGAGCGGCCTCGGCATCCGCAACCCTTACACCATCCGGATCATCATGGAGACGGTAAAGGTGCCGGTCATCGT
TGACGCCGGCGTGGGAACGGCCTCTGACGCGGCCATTGCCATGGAACTGGGGGTTGACGGCGTCCTCATGAACACCGGCA
TCGCCGGCGCCCAGAATCCCATCGCCATGGCCGAGGCCATGAACCTGGCGGTGCGGGCCGGGCGGCTCGCCTACCGGGCG
GGGCGCATCCCGAAAAAGCTCTACGCCACCGCATCGAGCCCCATCGAGGGGATGATTGAGTAA

Upstream 100 bases:

>100_bases
CATTCTTCCCAAGGGTGAATACGAATCCACCATCCTCAACGACGGAGACCGGATCGAGATCGTCCACTTTGTGGGCGGGG
GATAACAAAGGAGACTACCC

Downstream 100 bases:

>100_bases
GGTTGACTTTTCCCTCTACCTCATCACCGACCGTCGCCAGACGACTGGCCGGGACCTCCCCGCCGTGGTGGAGGAGGCCC
TGGCAGGGGGAGTGCGGGCC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSTTNDKLIIAGREFSSRLMVGTGKYASNEQMVAALEASGAEIITVAVRRVNITDRSKESLLDFIDTRKYTLLPNTAGCY
TADDAVRTCRLAREAGMSDMVKLEVLGNETTLYPDNEELLKAAKILVKDGFTVLPYTSDDPIICKKLEDIGCAAVMPLGA
PIGSGLGIRNPYTIRIIMETVKVPVIVDAGVGTASDAAIAMELGVDGVLMNTGIAGAQNPIAMAEAMNLAVRAGRLAYRA
GRIPKKLYATASSPIEGMIE

Sequences:

>Translated_260_residues
MSTTNDKLIIAGREFSSRLMVGTGKYASNEQMVAALEASGAEIITVAVRRVNITDRSKESLLDFIDTRKYTLLPNTAGCY
TADDAVRTCRLAREAGMSDMVKLEVLGNETTLYPDNEELLKAAKILVKDGFTVLPYTSDDPIICKKLEDIGCAAVMPLGA
PIGSGLGIRNPYTIRIIMETVKVPVIVDAGVGTASDAAIAMELGVDGVLMNTGIAGAQNPIAMAEAMNLAVRAGRLAYRA
GRIPKKLYATASSPIEGMIE
>Mature_259_residues
STTNDKLIIAGREFSSRLMVGTGKYASNEQMVAALEASGAEIITVAVRRVNITDRSKESLLDFIDTRKYTLLPNTAGCYT
ADDAVRTCRLAREAGMSDMVKLEVLGNETTLYPDNEELLKAAKILVKDGFTVLPYTSDDPIICKKLEDIGCAAVMPLGAP
IGSGLGIRNPYTIRIIMETVKVPVIVDAGVGTASDAAIAMELGVDGVLMNTGIAGAQNPIAMAEAMNLAVRAGRLAYRAG
RIPKKLYATASSPIEGMIE

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=47.4308300395257, Blast_Score=237, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_GEOMG (Q39RH2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385877.1
- ProteinModelPortal:   Q39RH2
- SMR:   Q39RH2
- STRING:   Q39RH2
- GeneID:   3740828
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2934
- NMPDR:   fig|269799.3.peg.3166
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q39RH2
- ProtClustDB:   PRK00208
- BioCyc:   GMET269799:GMET_2934-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27648; Mature: 27517

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTNDKLIIAGREFSSRLMVGTGKYASNEQMVAALEASGAEIITVAVRRVNITDRSKES
CCCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCHHH
LLDFIDTRKYTLLPNTAGCYTADDAVRTCRLAREAGMSDMVKLEVLGNETTLYPDNEELL
HHHHHHCCCEEECCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHHH
KAAKILVKDGFTVLPYTSDDPIICKKLEDIGCAAVMPLGAPIGSGLGIRNPYTIRIIMET
HHHHHHHHCCCEEEEECCCCCCEEEHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEE
VKVPVIVDAGVGTASDAAIAMELGVDGVLMNTGIAGAQNPIAMAEAMNLAVRAGRLAYRA
CCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GRIPKKLYATASSPIEGMIE
CCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STTNDKLIIAGREFSSRLMVGTGKYASNEQMVAALEASGAEIITVAVRRVNITDRSKES
CCCCCEEEEECCCCCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCHHH
LLDFIDTRKYTLLPNTAGCYTADDAVRTCRLAREAGMSDMVKLEVLGNETTLYPDNEELL
HHHHHHCCCEEECCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCEEECCCCHHHH
KAAKILVKDGFTVLPYTSDDPIICKKLEDIGCAAVMPLGAPIGSGLGIRNPYTIRIIMET
HHHHHHHHCCCEEEEECCCCCCEEEHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEE
VKVPVIVDAGVGTASDAAIAMELGVDGVLMNTGIAGAQNPIAMAEAMNLAVRAGRLAYRA
CCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GRIPKKLYATASSPIEGMIE
CCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA