Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 78224059

GI number: 78224059

Start: 3248058

End: 3248945

Strand: Reverse

Name: nadE [H]

Synonym: Gmet_2863

Alternate gene names: 78224059

Gene position: 3248945-3248058 (Counterclockwise)

Preceding gene: 78224060

Following gene: 78224058

Centisome position: 81.28

GC content: 56.08

Gene sequence:

>888_bases
ATGCTACCTTTGACCGAAAAATCGGGGGCTTATACCGTGGATTTCATTGTCGCACTGGCGCAGATAAAGCCGAAGCTCGG
CTGTCTTACTGAAAACTGCACCCTTGTCGAGTCCGCCATTGAGCGTGCTGTCGCTGATAAGGCTGACCTGATCGTGTTTC
CTGAGCTTGCTCTAACCGGCTATTTTCTCAGGGATCTGGTGCCGGATGTGGCGTTGCGCCTCGATGCCCCGGAGATCGCC
CGTATCCGCGAACTTTCCCGTCATGTCGCGATCGTTGTCGGCTTTGTGGAGGTCTCCGACGATTATCGCTTCTTCAATTC
CGCGATCTATGTGGATGGCGGCGAAATCCGACACATCCATCGCAAGGTCTACCTCCCCACCTATGGCCTCTTTGACGAGC
AGCGTTATCTTGCCCGCGGTGAACGGTTCCGCGCCTTCGATACCCGTTTCGGTCGGATGGGGATGCTCATCTGCGAAGAC
ATGTGGCATCTCTCCGCTCCCTATATCCTTGCCATGGATGGGGCTACAACCCTTGTATGTCTTTCCAGCAGCCCGGGTCG
GGGGATTAGCGAGGACGAAAGCCTGGGTTCAACCGCTGCCTGGCAGAAACTCACCTCCACGACGGCAATGTTTCTCAACA
GCCGCGTGTTTTATTGCAACAGGGTCGGCTACGAAGACGGCGTCAACTTCTGGGGGGGATCCGAAGTGATTGCTCCCTCC
GGCAGTGTTGTTTCCCGTGCCCGGTTGTTCGACGAAGATCTGCTTGTCACCCGTGTCGATGGAGGAGAGTTGCGGCGTGA
GCGCATTTTTTCCCCAATGATGCGCGACGAGAGCCTCTCTATTACCATGAAGGAGTTGCGGCGTATTGAGAAGGGGAGGG
ACTGTTGA

Upstream 100 bases:

>100_bases
CGATTACGTTCTCGGGTCAGATGCCACAAATCGAGCATATTCCCAATGCCTTCGAATTGGCGTATTAGCCCGGAAATACC
GTTGAAAACGGCAGTTTGCT

Downstream 100 bases:

>100_bases
TGGCTGACGTTAATGTCAAGCTGTTGCGTCGGATACTGGTCGGTTTTATCCGTGAGGAAGTCACCAAGGTTGGAATTCGC
AAGGCGGTTCTCGGCCTCTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLPLTEKSGAYTVDFIVALAQIKPKLGCLTENCTLVESAIERAVADKADLIVFPELALTGYFLRDLVPDVALRLDAPEIA
RIRELSRHVAIVVGFVEVSDDYRFFNSAIYVDGGEIRHIHRKVYLPTYGLFDEQRYLARGERFRAFDTRFGRMGMLICED
MWHLSAPYILAMDGATTLVCLSSSPGRGISEDESLGSTAAWQKLTSTTAMFLNSRVFYCNRVGYEDGVNFWGGSEVIAPS
GSVVSRARLFDEDLLVTRVDGGELRRERIFSPMMRDESLSITMKELRRIEKGRDC

Sequences:

>Translated_295_residues
MLPLTEKSGAYTVDFIVALAQIKPKLGCLTENCTLVESAIERAVADKADLIVFPELALTGYFLRDLVPDVALRLDAPEIA
RIRELSRHVAIVVGFVEVSDDYRFFNSAIYVDGGEIRHIHRKVYLPTYGLFDEQRYLARGERFRAFDTRFGRMGMLICED
MWHLSAPYILAMDGATTLVCLSSSPGRGISEDESLGSTAAWQKLTSTTAMFLNSRVFYCNRVGYEDGVNFWGGSEVIAPS
GSVVSRARLFDEDLLVTRVDGGELRRERIFSPMMRDESLSITMKELRRIEKGRDC
>Mature_295_residues
MLPLTEKSGAYTVDFIVALAQIKPKLGCLTENCTLVESAIERAVADKADLIVFPELALTGYFLRDLVPDVALRLDAPEIA
RIRELSRHVAIVVGFVEVSDDYRFFNSAIYVDGGEIRHIHRKVYLPTYGLFDEQRYLARGERFRAFDTRFGRMGMLICED
MWHLSAPYILAMDGATTLVCLSSSPGRGISEDESLGSTAAWQKLTSTTAMFLNSRVFYCNRVGYEDGVNFWGGSEVIAPS
GSVVSRARLFDEDLLVTRVDGGELRRERIFSPMMRDESLSITMKELRRIEKGRDC

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 33179; Mature: 33179

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLTEKSGAYTVDFIVALAQIKPKLGCLTENCTLVESAIERAVADKADLIVFPELALTG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHH
YFLRDLVPDVALRLDAPEIARIRELSRHVAIVVGFVEVSDDYRFFNSAIYVDGGEIRHIH
HHHHHHHHHHHEECCCHHHHHHHHHHHCEEEEEEEEEECCCCEEECCEEEEECCCEEEEE
RKVYLPTYGLFDEQRYLARGERFRAFDTRFGRMGMLICEDMWHLSAPYILAMDGATTLVC
EEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCEEEEE
LSSSPGRGISEDESLGSTAAWQKLTSTTAMFLNSRVFYCNRVGYEDGVNFWGGSEVIAPS
EECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEECCC
GSVVSRARLFDEDLLVTRVDGGELRRERIFSPMMRDESLSITMKELRRIEKGRDC
CCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCC
>Mature Secondary Structure
MLPLTEKSGAYTVDFIVALAQIKPKLGCLTENCTLVESAIERAVADKADLIVFPELALTG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHHHHH
YFLRDLVPDVALRLDAPEIARIRELSRHVAIVVGFVEVSDDYRFFNSAIYVDGGEIRHIH
HHHHHHHHHHHEECCCHHHHHHHHHHHCEEEEEEEEEECCCCEEECCEEEEECCCEEEEE
RKVYLPTYGLFDEQRYLARGERFRAFDTRFGRMGMLICEDMWHLSAPYILAMDGATTLVC
EEEECCCCCCCCCHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCEEEEE
LSSSPGRGISEDESLGSTAAWQKLTSTTAMFLNSRVFYCNRVGYEDGVNFWGGSEVIAPS
EECCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEECCC
GSVVSRARLFDEDLLVTRVDGGELRRERIFSPMMRDESLSITMKELRRIEKGRDC
CCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]