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The map label for this gene is nadE

Identifier: 78224058

GI number: 78224058

Start: 3247243

End: 3248058

Strand: Reverse

Name: nadE

Synonym: Gmet_2862

Alternate gene names: 78224058

Gene position: 3248058-3247243 (Counterclockwise)

Preceding gene: 78224059

Following gene: 78224057

Centisome position: 81.25

GC content: 54.53

Gene sequence:

>816_bases
ATGGCTGACGTTAATGTCAAGCTGTTGCGTCGGATACTGGTCGGTTTTATCCGTGAGGAAGTCACCAAGGTTGGAATTCG
CAAGGCGGTTCTCGGCCTCTCTGGAGGGATCGACTCCGCTCTCGTTGCGTATCTCGCTGCCGAGGCCCTCGGCCCTGAAA
ATATTCATGCCTGCATCATGCCGTATAGAACCAGTAATCCTGAAAGCGAGGCCCACGCAAGGCTCGCCGCGGAGCATCTC
GGCATTCACTATCAGGTAATCGACATTACGCCGATGATAGATGCCTATTTTTCACGCTTCCCCGATGCCGATAGCATGCG
TCGCGGAAACAAGATGGCCCGCGAACGGATGACAATTCTCTACGACCACTCGGCGGCACTTCCTGCCCTTGTCCTCGGAA
CAAGCAACAAGACGGAGCTCTTGCTTGGCTATGGGACCCTGTATGGAGACATGGCCAGCGCCCTGAATCCCATCGGTGAC
ATTTACAAAACGCAGGTCTGGCAGCTTTCCGAAGAGATGGGAGTTCCCCGCGACATCATTGAAAAACAGCCTTCCGCTGA
TTTGTGGGCCGGCCAAACCGACGAGCAAGAACTGGGATTCACCTATCGTGAGGTTGATAAGCTCCTCTATTCCATGGTTG
ATCAGCGGATGTCCCGCGAAGAACTGGAGAAGAACGGCTTTGCCGCTGATTTCATTGCCACCGTTTCGGCCAGGATACAG
AACTCCCACTTCAAACGACGCCTCCCCATCATCGCCAAGGTATCCAACCGGACCATTGATCGGGACTTCCGCTATTCCCG
CGACTGGGGTAAATGA

Upstream 100 bases:

>100_bases
AGTTGCGGCGTGAGCGCATTTTTTCCCCAATGATGCGCGACGAGAGCCTCTCTATTACCATGAAGGAGTTGCGGCGTATT
GAGAAGGGGAGGGACTGTTG

Downstream 100 bases:

>100_bases
AAACTCCGGGCACCCTCTATATTGTCGCTACCCCTATTGGAAATCTCGAGGACATCACCTTTCGGGCTGTGCGCATTCTG
AAAGAGGTTGATCTCATTGC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MADVNVKLLRRILVGFIREEVTKVGIRKAVLGLSGGIDSALVAYLAAEALGPENIHACIMPYRTSNPESEAHARLAAEHL
GIHYQVIDITPMIDAYFSRFPDADSMRRGNKMARERMTILYDHSAALPALVLGTSNKTELLLGYGTLYGDMASALNPIGD
IYKTQVWQLSEEMGVPRDIIEKQPSADLWAGQTDEQELGFTYREVDKLLYSMVDQRMSREELEKNGFAADFIATVSARIQ
NSHFKRRLPIIAKVSNRTIDRDFRYSRDWGK

Sequences:

>Translated_271_residues
MADVNVKLLRRILVGFIREEVTKVGIRKAVLGLSGGIDSALVAYLAAEALGPENIHACIMPYRTSNPESEAHARLAAEHL
GIHYQVIDITPMIDAYFSRFPDADSMRRGNKMARERMTILYDHSAALPALVLGTSNKTELLLGYGTLYGDMASALNPIGD
IYKTQVWQLSEEMGVPRDIIEKQPSADLWAGQTDEQELGFTYREVDKLLYSMVDQRMSREELEKNGFAADFIATVSARIQ
NSHFKRRLPIIAKVSNRTIDRDFRYSRDWGK
>Mature_270_residues
ADVNVKLLRRILVGFIREEVTKVGIRKAVLGLSGGIDSALVAYLAAEALGPENIHACIMPYRTSNPESEAHARLAAEHLG
IHYQVIDITPMIDAYFSRFPDADSMRRGNKMARERMTILYDHSAALPALVLGTSNKTELLLGYGTLYGDMASALNPIGDI
YKTQVWQLSEEMGVPRDIIEKQPSADLWAGQTDEQELGFTYREVDKLLYSMVDQRMSREELEKNGFAADFIATVSARIQN
SHFKRRLPIIAKVSNRTIDRDFRYSRDWGK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=211, Percent_Identity=29.8578199052133, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30512; Mature: 30381

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVNVKLLRRILVGFIREEVTKVGIRKAVLGLSGGIDSALVAYLAAEALGPENIHACIM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
PYRTSNPESEAHARLAAEHLGIHYQVIDITPMIDAYFSRFPDADSMRRGNKMARERMTIL
ECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
YDHSAALPALVLGTSNKTELLLGYGTLYGDMASALNPIGDIYKTQVWQLSEEMGVPRDII
ECCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EKQPSADLWAGQTDEQELGFTYREVDKLLYSMVDQRMSREELEKNGFAADFIATVSARIQ
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NSHFKRRLPIIAKVSNRTIDRDFRYSRDWGK
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADVNVKLLRRILVGFIREEVTKVGIRKAVLGLSGGIDSALVAYLAAEALGPENIHACIM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEE
PYRTSNPESEAHARLAAEHLGIHYQVIDITPMIDAYFSRFPDADSMRRGNKMARERMTIL
ECCCCCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHE
YDHSAALPALVLGTSNKTELLLGYGTLYGDMASALNPIGDIYKTQVWQLSEEMGVPRDII
ECCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
EKQPSADLWAGQTDEQELGFTYREVDKLLYSMVDQRMSREELEKNGFAADFIATVSARIQ
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
NSHFKRRLPIIAKVSNRTIDRDFRYSRDWGK
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA