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The map label for this gene is cysD [H]

Identifier: 78224055

GI number: 78224055

Start: 3238071

End: 3238976

Strand: Direct

Name: cysD [H]

Synonym: Gmet_2859

Alternate gene names: 78224055

Gene position: 3238071-3238976 (Clockwise)

Preceding gene: 78224054

Following gene: 78224056

Centisome position: 81.0

GC content: 60.6

Gene sequence:

>906_bases
ATGAGAACCACGCTGACCCACCTGCAGCAGCTCGAAGCAGAAAGCATCCACATCATCCGAGAGGTGGTGGCCGAATTTGC
CAACCCGGTGATGCTCTACTCCATCGGCAAGGATTCGGCGGTCATGCTGCACCTGGCACGCAAGGCCTTTTACCCGGCGC
CGCCGCCGTTTCCCCTGCTGCACGTGGATACCACCTGGAAGTTCCGCGACATGATCCAATTCCGCGACCGGATGGCGGCC
GAATGCGGCTTCGACCTGATCGTCCATGTGAACAAAGAGGGGGTGGAACAGGGGATTTCCCCATTCACCCACGGTTCAGC
CCTCTACACCGATATCATGAAGACCGAGGGGTTGAAGCAGGCACTGGACAAGTACCGGTTCGACGCCGCCTTCGGTGGTG
CCCGCCGTGACGAAGAAAAATCGCGGGCCAAGGAGAGGATCTTCTCCTTCCGCAGCGCCAACCACCGCTGGGATCCCAAA
AACCAGCGCCCGGAACTCTGGAACCTTTACAACACCCGTATCAAGCAGGGGGAAAGCATCCGGGTCTTCCCGCTCTCCAA
CTGGACCGAGTTGGATGTCTGGCAGTACATCCACCTGGAGAATATCCCGATCGTCCCCCTCTACTATGCTGCGGTGCGGC
CGGTGGTGGAGCGCGACGGCATGCTGATCATGGTGGACGACGAACGCCTGGAACTCAAGCCGGGCGAGACCGTGCAGCAC
AAGTCGGTACGCTTCCGCACCCTCGGCTGCTACCCCCTCACCGGCGCAGTGGAGTCAACGGCTGACACCCTCCCCAAGAT
CATCCAGGAGATGCTCCTGACCCGCACCTCCGAGCGCCAGGGGCGACTCATCGACCACGACCAAGCGGGCTCAATGGAGA
AGAAGAAGCAGGAGGGGTATTTCTAA

Upstream 100 bases:

>100_bases
ATCCCCGAGCCGGACGATGGTGGTGGGAGAATCCGGAGCATAAAGAGTGCGGGTTGCACCGGCACTGATGGAAGGCACAA
TCGTCTACGAGGATAACGAC

Downstream 100 bases:

>100_bases
TGGCACATCAATCGGAACTGATCGAAAAGGATATCCTCGCCTATCTCAAAAGCCAGGAGGAGAAATCGCTGCTCCGCTTC
ATCACCTGCGGCAGCGTTGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRTTLTHLQQLEAESIHIIREVVAEFANPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFRDMIQFRDRMAA
ECGFDLIVHVNKEGVEQGISPFTHGSALYTDIMKTEGLKQALDKYRFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPK
NQRPELWNLYNTRIKQGESIRVFPLSNWTELDVWQYIHLENIPIVPLYYAAVRPVVERDGMLIMVDDERLELKPGETVQH
KSVRFRTLGCYPLTGAVESTADTLPKIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF

Sequences:

>Translated_301_residues
MRTTLTHLQQLEAESIHIIREVVAEFANPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFRDMIQFRDRMAA
ECGFDLIVHVNKEGVEQGISPFTHGSALYTDIMKTEGLKQALDKYRFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPK
NQRPELWNLYNTRIKQGESIRVFPLSNWTELDVWQYIHLENIPIVPLYYAAVRPVVERDGMLIMVDDERLELKPGETVQH
KSVRFRTLGCYPLTGAVESTADTLPKIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF
>Mature_301_residues
MRTTLTHLQQLEAESIHIIREVVAEFANPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLLHVDTTWKFRDMIQFRDRMAA
ECGFDLIVHVNKEGVEQGISPFTHGSALYTDIMKTEGLKQALDKYRFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPK
NQRPELWNLYNTRIKQGESIRVFPLSNWTELDVWQYIHLENIPIVPLYYAAVRPVVERDGMLIMVDDERLELKPGETVQH
KSVRFRTLGCYPLTGAVESTADTLPKIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=76.4309764309764, Blast_Score=480, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34866; Mature: 34866

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTLTHLQQLEAESIHIIREVVAEFANPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFRDMIQFRDRMAAECGFDLIVHVNKEGVEQGISPFTHGSALYTDIMKTEGLKQ
EECCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ALDKYRFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPKNQRPELWNLYNTRIKQGESI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
RVFPLSNWTELDVWQYIHLENIPIVPLYYAAVRPVVERDGMLIMVDDERLELKPGETVQH
EEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCC
KSVRFRTLGCYPLTGAVESTADTLPKIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGY
CCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCC
F
C
>Mature Secondary Structure
MRTTLTHLQQLEAESIHIIREVVAEFANPVMLYSIGKDSAVMLHLARKAFYPAPPPFPLL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCEE
HVDTTWKFRDMIQFRDRMAAECGFDLIVHVNKEGVEQGISPFTHGSALYTDIMKTEGLKQ
EECCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
ALDKYRFDAAFGGARRDEEKSRAKERIFSFRSANHRWDPKNQRPELWNLYNTRIKQGESI
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCE
RVFPLSNWTELDVWQYIHLENIPIVPLYYAAVRPVVERDGMLIMVDDERLELKPGETVQH
EEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEECCCCCCCC
KSVRFRTLGCYPLTGAVESTADTLPKIIQEMLLTRTSERQGRLIDHDQAGSMEKKKQEGY
CCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCC
F
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA