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The map label for this gene is ilvK [H]

Identifier: 78224049

GI number: 78224049

Start: 3231731

End: 3232804

Strand: Reverse

Name: ilvK [H]

Synonym: Gmet_2853

Alternate gene names: 78224049

Gene position: 3232804-3231731 (Counterclockwise)

Preceding gene: 78224057

Following gene: 78224048

Centisome position: 80.87

GC content: 61.17

Gene sequence:

>1074_bases
ATGGAGATCAGAATCGCGCCGCTCCCGGAGGGCCGGAAAAAGCCCAAGTACACCGACGAATCCCAGCTCGGTTTCGGCAA
GCTCTTTACCGACCGGATGTTTATGGTCGAGTGGAAAATCGGCCAGGGGTGGGTCGATGCCCGGATTGAGCCCTATGCCC
CCTTCGTCCTCGATCCGGCCTGCTCTGTTCTTCACTATGCCCAGGAGATCTTCGAGGGGCTCAAGGCCTACAAGTGGGCC
GACGGCACCATCACCCTCTTCCGCCCCGAGATGAACGCTCGACGTTTCAACCACTCAGCCGATCGCCTCTGCATGCCAGA
GGTGCCGGAGGAGCTTTTCCTTGACGGCATCGAGAAGCTCGTGGCGTTGGAGCGCGACTGGATTCCCTCGTCGGAAGGGA
CCTCCCTCTACATCCGTCCCACCATGATCGGCGTGGACCCGGTCCTGGGGGTGAAGCCCGCTGATCACTACTACTTCTAC
GTGATCCTCTCTCCTGTTGGTGCGTATTACTCCGCGGGGTTCAACCCCATCAAGATCATGGTGGAGGACCAGTACGTCCG
CTCCGTCCCCGGCGGCACCGGGGAGGCCAAGACCGGCGGCAATTACGCCTGCTCCCTCAAGGCCGGCCTTGAGGCGAAGA
AAAAGGGGTATGACCAGGTCCTCTGGCTCGACGGGGTCCACCGTCGCTATATTGAGGAAGTGGGGGCCATGAATATGCTC
TTCGCCTATGGGAAGACCATTGTCACCGCTTCTCTCACCGGCACGATCCTGAACGGCGTCACCCGCGACTCCACCCTGAA
GCTGGCTGCGAGCCTCGGCTACACCATCGAGGAGCGCCTCATCGACGTGAATGAGCTCATGGCGGACATTCGTGCCGGCA
AGGTGACCGAGGCTTTCGGCAGCGGCACTGCTGCAGTCATCAGCCCGGTCGGCGTTCTTGCGTACAAGGACGAAGTCTGT
ACGGTCGGCAATGGCGGCGTGGGGCCGATTACCCAGCAGCTCTACGATACTCTGACCGGCATTCAGACCGGCAAGGTTCC
CGACACCTTCGGCTGGACCCGCAAGATCGCATGA

Upstream 100 bases:

>100_bases
TGCCGGGGGAAACCCCTTGCCCCCTGTGGTATTCATGTGATAAACCGTAAAGAGTTTCATATTACGACGCTCCGCGCCGA
ATCAATGGAGGTTTCAGCAG

Downstream 100 bases:

>100_bases
TTCAGAGGGAATGGGCGGCGGAAACGCCGCCTTTTCTTTTGTCCCCACTTTCTGCTACTATTGAGGCAGCGGAGATATCA
TTCGCACATCTCTATGAAGG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT 2; Vegetative protein 85; VEG85 [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MEIRIAPLPEGRKKPKYTDESQLGFGKLFTDRMFMVEWKIGQGWVDARIEPYAPFVLDPACSVLHYAQEIFEGLKAYKWA
DGTITLFRPEMNARRFNHSADRLCMPEVPEELFLDGIEKLVALERDWIPSSEGTSLYIRPTMIGVDPVLGVKPADHYYFY
VILSPVGAYYSAGFNPIKIMVEDQYVRSVPGGTGEAKTGGNYACSLKAGLEAKKKGYDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTASLTGTILNGVTRDSTLKLAASLGYTIEERLIDVNELMADIRAGKVTEAFGSGTAAVISPVGVLAYKDEVC
TVGNGGVGPITQQLYDTLTGIQTGKVPDTFGWTRKIA

Sequences:

>Translated_357_residues
MEIRIAPLPEGRKKPKYTDESQLGFGKLFTDRMFMVEWKIGQGWVDARIEPYAPFVLDPACSVLHYAQEIFEGLKAYKWA
DGTITLFRPEMNARRFNHSADRLCMPEVPEELFLDGIEKLVALERDWIPSSEGTSLYIRPTMIGVDPVLGVKPADHYYFY
VILSPVGAYYSAGFNPIKIMVEDQYVRSVPGGTGEAKTGGNYACSLKAGLEAKKKGYDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTASLTGTILNGVTRDSTLKLAASLGYTIEERLIDVNELMADIRAGKVTEAFGSGTAAVISPVGVLAYKDEVC
TVGNGGVGPITQQLYDTLTGIQTGKVPDTFGWTRKIA
>Mature_357_residues
MEIRIAPLPEGRKKPKYTDESQLGFGKLFTDRMFMVEWKIGQGWVDARIEPYAPFVLDPACSVLHYAQEIFEGLKAYKWA
DGTITLFRPEMNARRFNHSADRLCMPEVPEELFLDGIEKLVALERDWIPSSEGTSLYIRPTMIGVDPVLGVKPADHYYFY
VILSPVGAYYSAGFNPIKIMVEDQYVRSVPGGTGEAKTGGNYACSLKAGLEAKKKGYDQVLWLDGVHRRYIEEVGAMNML
FAYGKTIVTASLTGTILNGVTRDSTLKLAASLGYTIEERLIDVNELMADIRAGKVTEAFGSGTAAVISPVGVLAYKDEVC
TVGNGGVGPITQQLYDTLTGIQTGKVPDTFGWTRKIA

Specific function: Transaminates branched-chain amino acids and ketoglutarate [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=352, Percent_Identity=41.1931818181818, Blast_Score=254, Evalue=6e-68,
Organism=Homo sapiens, GI296010906, Length=352, Percent_Identity=41.1931818181818, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI296010904, Length=352, Percent_Identity=41.1931818181818, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI50658084, Length=330, Percent_Identity=40.3030303030303, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI296010902, Length=352, Percent_Identity=36.6477272727273, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI296010900, Length=352, Percent_Identity=36.6477272727273, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI258614015, Length=284, Percent_Identity=38.3802816901408, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI48994963, Length=319, Percent_Identity=32.6018808777429, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=370, Percent_Identity=38.3783783783784, Blast_Score=257, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17565728, Length=349, Percent_Identity=33.5243553008596, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322608, Length=356, Percent_Identity=41.8539325842697, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322002, Length=357, Percent_Identity=41.4565826330532, Blast_Score=263, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24641779, Length=355, Percent_Identity=38.8732394366197, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39353; Mature: 39353

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIRIAPLPEGRKKPKYTDESQLGFGKLFTDRMFMVEWKIGQGWVDARIEPYAPFVLDPA
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCEECCCCCCCCCEEECHH
CSVLHYAQEIFEGLKAYKWADGTITLFRPEMNARRFNHSADRLCMPEVPEELFLDGIEKL
HHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VALERDWIPSSEGTSLYIRPTMIGVDPVLGVKPADHYYFYVILSPVGAYYSAGFNPIKIM
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDQYVRSVPGGTGEAKTGGNYACSLKAGLEAKKKGYDQVLWLDGVHRRYIEEVGAMNML
EECHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHCCCCEEEEECHHHHHHHHHHHHHHHH
FAYGKTIVTASLTGTILNGVTRDSTLKLAASLGYTIEERLIDVNELMADIRAGKVTEAFG
HHHCCEEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHC
SGTAAVISPVGVLAYKDEVCTVGNGGVGPITQQLYDTLTGIQTGKVPDTFGWTRKIA
CCCCHHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEIRIAPLPEGRKKPKYTDESQLGFGKLFTDRMFMVEWKIGQGWVDARIEPYAPFVLDPA
CEEEECCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEEEECCCEECCCCCCCCCEEECHH
CSVLHYAQEIFEGLKAYKWADGTITLFRPEMNARRFNHSADRLCMPEVPEELFLDGIEKL
HHHHHHHHHHHHHHHHEEECCCEEEEECCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VALERDWIPSSEGTSLYIRPTMIGVDPVLGVKPADHYYFYVILSPVGAYYSAGFNPIKIM
HHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCEEEEE
VEDQYVRSVPGGTGEAKTGGNYACSLKAGLEAKKKGYDQVLWLDGVHRRYIEEVGAMNML
EECHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHCCCCEEEEECHHHHHHHHHHHHHHHH
FAYGKTIVTASLTGTILNGVTRDSTLKLAASLGYTIEERLIDVNELMADIRAGKVTEAFG
HHHCCEEEEEEHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHC
SGTAAVISPVGVLAYKDEVCTVGNGGVGPITQQLYDTLTGIQTGKVPDTFGWTRKIA
CCCCHHHCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 7934828; 9298659 [H]