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The map label for this gene is pth

Identifier: 78224042

GI number: 78224042

Start: 3224555

End: 3225139

Strand: Reverse

Name: pth

Synonym: Gmet_2846

Alternate gene names: 78224042

Gene position: 3225139-3224555 (Counterclockwise)

Preceding gene: 78224043

Following gene: 78224041

Centisome position: 80.68

GC content: 57.61

Gene sequence:

>585_bases
ATGGCTACAAGACTTATAATCGGGCTGGGAAATCCCGGCCCGAAGTACCAATGGACCCGCCATAACGCGGGTTTCATGGT
TTTAGACCACCTTTCCCGCGTTATGGGGACTTCGGTCGCCAAGAAGAGCTTCTCCGGGCTGTATGGTGAGGGAAGCTGGC
ATGGTGATCGCCTGCTGCTGCTCAAGCCACAGACCTTCATGAATCTCTCCGGGAGATCCGCGGCCGAGGCACTCCGCTTC
CACAAGCTGACCCTTTCCGACCTGATCGTGATCCATGACGACCTCGACATCCCCTTTGGCCGAGTAAAGCTCAAGGAGGG
GGGAGGGCATGGTGGGCATAACGGTCTCCGTTCCCTTATGCAGGAGTTGGGTGGCGGCGGTTTCGTGCGGATCAGAATCG
GTGTGGGGAGGCCCTTGCGCGGCGATGCCGCCGATTATGTCCTCGCCAATTTTTCTTCTGCAGAAATGGCCGGTCTTCCT
GCGCTCCTGGACGGTGTGGTTGATCTTCTCGGGGAACTCGTAAGGAACGGACTCCCGCGGACCATGAGCCTTTATAACAA
CAAGGATTTTCTACCGGCAGCCTGA

Upstream 100 bases:

>100_bases
CCCTCGACGATCCGAAGGCTACCATCGTTAGCGTCCATGGCAAGACCAAGGAAGAGGTAACTGTCGAGGAGGGGTAACCT
CCTCGCCGCATAGTGCACTA

Downstream 100 bases:

>100_bases
TCTCCCTCCTGCTGTCATTCCAACGCGAAAGAAGAATGCACCATGGGTTTTAACTGCGGTATCGTCGGACTTCCCAACGT
GGGAAAGTCTACCATCTTTA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MATRLIIGLGNPGPKYQWTRHNAGFMVLDHLSRVMGTSVAKKSFSGLYGEGSWHGDRLLLLKPQTFMNLSGRSAAEALRF
HKLTLSDLIVIHDDLDIPFGRVKLKEGGGHGGHNGLRSLMQELGGGGFVRIRIGVGRPLRGDAADYVLANFSSAEMAGLP
ALLDGVVDLLGELVRNGLPRTMSLYNNKDFLPAA

Sequences:

>Translated_194_residues
MATRLIIGLGNPGPKYQWTRHNAGFMVLDHLSRVMGTSVAKKSFSGLYGEGSWHGDRLLLLKPQTFMNLSGRSAAEALRF
HKLTLSDLIVIHDDLDIPFGRVKLKEGGGHGGHNGLRSLMQELGGGGFVRIRIGVGRPLRGDAADYVLANFSSAEMAGLP
ALLDGVVDLLGELVRNGLPRTMSLYNNKDFLPAA
>Mature_193_residues
ATRLIIGLGNPGPKYQWTRHNAGFMVLDHLSRVMGTSVAKKSFSGLYGEGSWHGDRLLLLKPQTFMNLSGRSAAEALRFH
KLTLSDLIVIHDDLDIPFGRVKLKEGGGHGGHNGLRSLMQELGGGGFVRIRIGVGRPLRGDAADYVLANFSSAEMAGLPA
LLDGVVDLLGELVRNGLPRTMSLYNNKDFLPAA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=176, Percent_Identity=38.0681818181818, Blast_Score=112, Evalue=2e-25,
Organism=Escherichia coli, GI1787455, Length=140, Percent_Identity=42.8571428571429, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321983, Length=140, Percent_Identity=32.1428571428571, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_GEOMG (Q39RR0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385789.1
- STRING:   Q39RR0
- GeneID:   3741152
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2846
- NMPDR:   fig|269799.3.peg.3016
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q39RR0
- ProtClustDB:   PRK05426
- BioCyc:   GMET269799:GMET_2846-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20948; Mature: 20816

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRLIIGLGNPGPKYQWTRHNAGFMVLDHLSRVMGTSVAKKSFSGLYGEGSWHGDRLLL
CCCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LKPQTFMNLSGRSAAEALRFHKLTLSDLIVIHDDLDIPFGRVKLKEGGGHGGHNGLRSLM
ECCHHHCCCCCCHHHHHHHHHHCCHHHEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHH
QELGGGGFVRIRIGVGRPLRGDAADYVLANFSSAEMAGLPALLDGVVDLLGELVRNGLPR
HHHCCCCEEEEEEECCCCCCCCCHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
TMSLYNNKDFLPAA
EEHHCCCCCCCCCC
>Mature Secondary Structure 
ATRLIIGLGNPGPKYQWTRHNAGFMVLDHLSRVMGTSVAKKSFSGLYGEGSWHGDRLLL
CCEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LKPQTFMNLSGRSAAEALRFHKLTLSDLIVIHDDLDIPFGRVKLKEGGGHGGHNGLRSLM
ECCHHHCCCCCCHHHHHHHHHHCCHHHEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHH
QELGGGGFVRIRIGVGRPLRGDAADYVLANFSSAEMAGLPALLDGVVDLLGELVRNGLPR
HHHCCCCEEEEEEECCCCCCCCCHHHHEECCCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
TMSLYNNKDFLPAA
EEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA