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The map label for this gene is engD [H]

Identifier: 78224041

GI number: 78224041

Start: 3223418

End: 3224512

Strand: Reverse

Name: engD [H]

Synonym: Gmet_2845

Alternate gene names: 78224041

Gene position: 3224512-3223418 (Counterclockwise)

Preceding gene: 78224042

Following gene: 78224040

Centisome position: 80.66

GC content: 58.81

Gene sequence:

>1095_bases
ATGGGTTTTAACTGCGGTATCGTCGGACTTCCCAACGTGGGAAAGTCTACCATCTTTAATGCACTGACCTCTGCTGGGGC
CGAGTCGGCAAATTATCCCTTCTGCACCATCGACCCCAACGTCGGCATCGTTCAGGTTCCCGACGAGCGGCTGGAGCGAT
TGGCTGAAATCGTCAATCCAGAGCGGATACTCCCCACCACCATCGAGTTCGTCGATATTGCGGGACTGGTCAAGGGAGCA
AGCCAAGGGGAGGGGCTCGGCAACCAGTTCCTCGGGCACATCCGTTCGGTGGATGCCATTGTCCATGTGGTCCGCTGTTT
TGATGACGAGAATGTGGTGCATGTGAGCGGGAGTGTTGATCCGATCCGTGATATCGAGGTGATTCAGACCGAACTGGCTC
TGGCTGATCTGGACAGCGTGGAGAAAAAATTCCAGCGCGTCGAGAAGCAGGCCAAGGGCGGTGACCGGAAGCTCAAGGAT
GAAAGCGAGTTTTACGCCCGCGTCAAGGGGGCGCTGGAGAAAGGCACTCCCGGGCGGCACGTTGAGGTGGGTGACGATGA
GCGCCTCTGGATGCGCGATCTGCATCTGCTGACCGATAAGCCGGTCCTCTACGTCGCCAACGTCGCCGAGGACGACCTGG
AGGGGGCGCACCCCTTTGTCGCCAAAGTGCGGGACCTGGCCGAGAGTGAAGGTGCCCGGGTCGTTGTTATCTGTGGTCGG
ATCGAGTCCGAGATAGCCGAACTCGACGGAGACGAAAAGAAGGTGTTTCTTGCCGAAATGGGGCTCGCCGAGTCGGGGCT
CGACCGCCTGATCCGGATGGGATACGATCTCCTCGGGCTCATTACCTATTTCACTGCTGGCAAGAAAGAGGTCCGTGCCT
GGACCATCCCGGCCGGCACCAAGGCGCCCCAGGCAGCTGGAGTCATCCACTCGGATTTCGAGAAGGGGTTTATTCGCGCC
GAAGTAATCGCCTACCGTGACTTTGTGGCTGCAGGCGGCGAGACGGGAGCCAAGGAAAAAGGGCTCATGCGCCTCGAAGG
GAAAGAGTACGTTGTTCAGGATGGCGACGTCATGCACTTCCGGTTCAACGTGTAG

Upstream 100 bases:

>100_bases
CGGACTCCCGCGGACCATGAGCCTTTATAACAACAAGGATTTTCTACCGGCAGCCTGATCTCCCTCCTGCTGTCATTCCA
ACGCGAAAGAAGAATGCACC

Downstream 100 bases:

>100_bases
ATAGTAAGTCGATTGGCCGCTTGGGCGCATATCACGATAAAATTCTTGAATAGCCGGGTAAGCTGTAGTAAATTTCTTTT
TTTGCAGCGGCCTTAGGCCG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MGFNCGIVGLPNVGKSTIFNALTSAGAESANYPFCTIDPNVGIVQVPDERLERLAEIVNPERILPTTIEFVDIAGLVKGA
SQGEGLGNQFLGHIRSVDAIVHVVRCFDDENVVHVSGSVDPIRDIEVIQTELALADLDSVEKKFQRVEKQAKGGDRKLKD
ESEFYARVKGALEKGTPGRHVEVGDDERLWMRDLHLLTDKPVLYVANVAEDDLEGAHPFVAKVRDLAESEGARVVVICGR
IESEIAELDGDEKKVFLAEMGLAESGLDRLIRMGYDLLGLITYFTAGKKEVRAWTIPAGTKAPQAAGVIHSDFEKGFIRA
EVIAYRDFVAAGGETGAKEKGLMRLEGKEYVVQDGDVMHFRFNV

Sequences:

>Translated_364_residues
MGFNCGIVGLPNVGKSTIFNALTSAGAESANYPFCTIDPNVGIVQVPDERLERLAEIVNPERILPTTIEFVDIAGLVKGA
SQGEGLGNQFLGHIRSVDAIVHVVRCFDDENVVHVSGSVDPIRDIEVIQTELALADLDSVEKKFQRVEKQAKGGDRKLKD
ESEFYARVKGALEKGTPGRHVEVGDDERLWMRDLHLLTDKPVLYVANVAEDDLEGAHPFVAKVRDLAESEGARVVVICGR
IESEIAELDGDEKKVFLAEMGLAESGLDRLIRMGYDLLGLITYFTAGKKEVRAWTIPAGTKAPQAAGVIHSDFEKGFIRA
EVIAYRDFVAAGGETGAKEKGLMRLEGKEYVVQDGDVMHFRFNV
>Mature_363_residues
GFNCGIVGLPNVGKSTIFNALTSAGAESANYPFCTIDPNVGIVQVPDERLERLAEIVNPERILPTTIEFVDIAGLVKGAS
QGEGLGNQFLGHIRSVDAIVHVVRCFDDENVVHVSGSVDPIRDIEVIQTELALADLDSVEKKFQRVEKQAKGGDRKLKDE
SEFYARVKGALEKGTPGRHVEVGDDERLWMRDLHLLTDKPVLYVANVAEDDLEGAHPFVAKVRDLAESEGARVVVICGRI
ESEIAELDGDEKKVFLAEMGLAESGLDRLIRMGYDLLGLITYFTAGKKEVRAWTIPAGTKAPQAAGVIHSDFEKGFIRAE
VIAYRDFVAAGGETGAKEKGLMRLEGKEYVVQDGDVMHFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=365, Percent_Identity=46.027397260274, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI58761502, Length=225, Percent_Identity=40.4444444444444, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI24308117, Length=149, Percent_Identity=30.8724832214765, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787454, Length=364, Percent_Identity=60.7142857142857, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1789574, Length=129, Percent_Identity=30.2325581395349, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=363, Percent_Identity=43.801652892562, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6319499, Length=370, Percent_Identity=39.7297297297297, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=37.7952755905512, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6321649, Length=161, Percent_Identity=37.2670807453416, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640873, Length=369, Percent_Identity=44.7154471544715, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640877, Length=369, Percent_Identity=44.7154471544715, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640875, Length=369, Percent_Identity=44.7154471544715, Blast_Score=309, Evalue=2e-84,
Organism=Drosophila melanogaster, GI24640879, Length=331, Percent_Identity=42.5981873111783, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24585318, Length=139, Percent_Identity=30.9352517985612, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39795; Mature: 39664

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFNCGIVGLPNVGKSTIFNALTSAGAESANYPFCTIDPNVGIVQVPDERLERLAEIVNP
CCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCC
ERILPTTIEFVDIAGLVKGASQGEGLGNQFLGHIRSVDAIVHVVRCFDDENVVHVSGSVD
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PIRDIEVIQTELALADLDSVEKKFQRVEKQAKGGDRKLKDESEFYARVKGALEKGTPGRH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
VEVGDDERLWMRDLHLLTDKPVLYVANVAEDDLEGAHPFVAKVRDLAESEGARVVVICGR
EECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
IESEIAELDGDEKKVFLAEMGLAESGLDRLIRMGYDLLGLITYFTAGKKEVRAWTIPAGT
HHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KAPQAAGVIHSDFEKGFIRAEVIAYRDFVAAGGETGAKEKGLMRLEGKEYVVQDGDVMHF
CCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEEE
RFNV
EECC
>Mature Secondary Structure 
GFNCGIVGLPNVGKSTIFNALTSAGAESANYPFCTIDPNVGIVQVPDERLERLAEIVNP
CCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHCCC
ERILPTTIEFVDIAGLVKGASQGEGLGNQFLGHIRSVDAIVHVVRCFDDENVVHVSGSVD
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
PIRDIEVIQTELALADLDSVEKKFQRVEKQAKGGDRKLKDESEFYARVKGALEKGTPGRH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
VEVGDDERLWMRDLHLLTDKPVLYVANVAEDDLEGAHPFVAKVRDLAESEGARVVVICGR
EECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
IESEIAELDGDEKKVFLAEMGLAESGLDRLIRMGYDLLGLITYFTAGKKEVRAWTIPAGT
HHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
KAPQAAGVIHSDFEKGFIRAEVIAYRDFVAAGGETGAKEKGLMRLEGKEYVVQDGDVMHF
CCCCCCCCHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEEEEECCCEEEE
RFNV
EECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]