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The map label for this gene is nfsA [C]

Identifier: 78224031

GI number: 78224031

Start: 3215048

End: 3215608

Strand: Reverse

Name: nfsA [C]

Synonym: Gmet_2835

Alternate gene names: 78224031

Gene position: 3215608-3215048 (Counterclockwise)

Preceding gene: 78224032

Following gene: 78224029

Centisome position: 80.44

GC content: 60.07

Gene sequence:

>561_bases
ATGGAGACACTGGAAGCAATCAGAACGAGGAGAAGCGTCCGCAAGTTTTCCGATCGGCCGGTGGAGCCCGAAAAGCTTCG
GGCGGTTCTGGATGCGGCGCGACTCGCTCCGTCGTGGGCAAACATGCAGTGCTGGCGGTTTGTTGTGGTGGAGGATCAGG
CAACGAAGGTGCAGATCAGCGAGCTCTCCTACGTGGAGGCCTACTTCGGCCCAAAGGGGTACAAGTCGAATCCGGCTCAG
AAGGCCCTGGCCGAAGCGCCGGTTGTGATCATCGCCTGCGGCGAACCGCCTCAATCCGGGGAGTTGCGGGGGCAGCAGTA
TTACCTGACCGATGTGGGGATTGCGGCCCAGAATCTCATGCTTGCCGCCCACGACCTGGGGCTGGGGAGCGTCTTTGTCG
GCGTCTTTGACGAGCAGCAACTGGGAGAACTTCTCGGTATCCCGGCGGAGCTCCGGATTGTTGGGCTCTTTCCTTTGGGA
TATCCCCTTGAGGGGCCGAAGGCTGGGCCGTCACGCAAGCCTTTGGATGAGATTGTCCACTATGGTAAGTATCAGGCGTA
A

Upstream 100 bases:

>100_bases
ATTCCCGGCAAGTGACGAGAACGGAGCGGCAACAACGCAGGCCGGCTCCGCAATAGTTTTGCAAGAGCCTCGCTATTCAA
CTGAGAAAGGAAACCTAACC

Downstream 100 bases:

>100_bases
ATCCTTCCGGCAACGACAGAGGGTGAATAAATGGGCGCGGAGAATAGATCTCCGCGCCTTTCCTTCAGCATTGGCGGAAG
GGGTTACTGCCCCAGGGCCG

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
METLEAIRTRRSVRKFSDRPVEPEKLRAVLDAARLAPSWANMQCWRFVVVEDQATKVQISELSYVEAYFGPKGYKSNPAQ
KALAEAPVVIIACGEPPQSGELRGQQYYLTDVGIAAQNLMLAAHDLGLGSVFVGVFDEQQLGELLGIPAELRIVGLFPLG
YPLEGPKAGPSRKPLDEIVHYGKYQA

Sequences:

>Translated_186_residues
METLEAIRTRRSVRKFSDRPVEPEKLRAVLDAARLAPSWANMQCWRFVVVEDQATKVQISELSYVEAYFGPKGYKSNPAQ
KALAEAPVVIIACGEPPQSGELRGQQYYLTDVGIAAQNLMLAAHDLGLGSVFVGVFDEQQLGELLGIPAELRIVGLFPLG
YPLEGPKAGPSRKPLDEIVHYGKYQA
>Mature_186_residues
METLEAIRTRRSVRKFSDRPVEPEKLRAVLDAARLAPSWANMQCWRFVVVEDQATKVQISELSYVEAYFGPKGYKSNPAQ
KALAEAPVVIIACGEPPQSGELRGQQYYLTDVGIAAQNLMLAAHDLGLGSVFVGVFDEQQLGELLGIPAELRIVGLFPLG
YPLEGPKAGPSRKPLDEIVHYGKYQA

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Homo sapiens, GI42794271, Length=195, Percent_Identity=30.7692307692308, Blast_Score=64, Evalue=6e-11,
Organism=Escherichia coli, GI1787075, Length=194, Percent_Identity=26.8041237113402, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 20456; Mature: 20456

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLEAIRTRRSVRKFSDRPVEPEKLRAVLDAARLAPSWANMQCWRFVVVEDQATKVQIS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHH
ELSYVEAYFGPKGYKSNPAQKALAEAPVVIIACGEPPQSGELRGQQYYLTDVGIAAQNLM
HHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHE
LAAHDLGLGSVFVGVFDEQQLGELLGIPAELRIVGLFPLGYPLEGPKAGPSRKPLDEIVH
EEHHHCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
YGKYQA
CCCCCC
>Mature Secondary Structure
METLEAIRTRRSVRKFSDRPVEPEKLRAVLDAARLAPSWANMQCWRFVVVEDQATKVQIS
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHH
ELSYVEAYFGPKGYKSNPAQKALAEAPVVIIACGEPPQSGELRGQQYYLTDVGIAAQNLM
HHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHE
LAAHDLGLGSVFVGVFDEQQLGELLGIPAELRIVGLFPLGYPLEGPKAGPSRKPLDEIVH
EEHHHCCCCCEEEEECCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHH
YGKYQA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]