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The map label for this gene is pdhB [H]

Identifier: 78223949

GI number: 78223949

Start: 3112755

End: 3113741

Strand: Reverse

Name: pdhB [H]

Synonym: Gmet_2753

Alternate gene names: 78223949

Gene position: 3113741-3112755 (Counterclockwise)

Preceding gene: 78223950

Following gene: 78223948

Centisome position: 77.89

GC content: 66.77

Gene sequence:

>987_bases
ATGCATGAAATGACCTACCGCGATGCCCTGAACAAGGCCCTGAAAGAAGAGATGCGGCGCGACCCGTCCGTGGTTGCCTG
GGGGGAGGACGTGGCCCTGTACGAGGGCTCCTTCAAGGTGACCCGGGGGCTTCTGGCCGAGTTCGGCGAGGAGCGGGTGA
AGGATACCCCCATCTCCGAGAACACCATCATCGGCGTCTCCATCGGGGCGGCCATGGGTGGCTTGAGGCCCGTGGCGGAA
CTGATGACGGTGAACTTCGCCCTCCTGGCCATGGACCAGATCGTGAACCACATGGCCAAGATCAGCTCCATGTTCGGGGG
GCAGACCCATCTCCCCATGGTGGTCCGCGCCCCGGGGGGCGGTGGGAGCCAGCTGGGGGCCCAGCACTCCCAGAGCCTGG
AAACCTACTTCATGCACTGCCCCGGCATCCATGTGGCGGTGCCGGCGACGCCTGCCGACGCCCGGGGGCTCCTGAAGGCC
TCCATCCGCGACAACAACCCGGTGATGTTCCTGGAGCACGAGCTCCTCTACAACAGCAAGGGGGAGGTGCCCGACGACCC
GGAGTTCCTGGTCCCCCTGGGGAAGGCCGAGGTGAAGCGGGAGGGGAAGGATGTGACCATCGTCGCCTATTCGCGCATGA
CCATCCTGGCGCTGCAGGCGGCGGCGGAACTGGAGAAGGAGGGGAGCTCCTGCGAGGTGGTGGACCTGCGGACCCTGGCG
CCCCTGGACACGGAGACCTTTGTTGCCTCGGTGCAGAAGACCGGCCGGGCCGTGGTGGTGGAGGAGTGCTGGCGCGCCGC
CGGCCTCGGGGGGCACCTGGCCTCCATCATCGCCGAGGAGTGCTTCGACCGGCTCCTGGCGCCGGTGCGGCGGGTGTCGG
GGCTCGACGTCCCCATGCCTTACTCGCGCAAGATCGAGAAGCTCTGCATCCCGCAGGTGGAGACCATCGCCGCAGCGGTT
CGGGACGTAATGAGTCAGAAATACTGA

Upstream 100 bases:

>100_bases
ATTAGTAGCAGAACAGGATGAGGTGCAGAATACTATTCAACCAAGCGCAACAGCGGTGAACCCGCTGTGCTCTCAGCCGT
TATAGTAACGGAGGTTTCCC

Downstream 100 bases:

>100_bases
CAAGGAGGCACGGATGCCAACCGATATCACCATGCCCAAACTCTCGGATACCATGACCGAAGGCCGGCTCGTTTCCTGGA
AGAAGAGCGTGGGCGAGCGA

Product: transketolase, central region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MHEMTYRDALNKALKEEMRRDPSVVAWGEDVALYEGSFKVTRGLLAEFGEERVKDTPISENTIIGVSIGAAMGGLRPVAE
LMTVNFALLAMDQIVNHMAKISSMFGGQTHLPMVVRAPGGGGSQLGAQHSQSLETYFMHCPGIHVAVPATPADARGLLKA
SIRDNNPVMFLEHELLYNSKGEVPDDPEFLVPLGKAEVKREGKDVTIVAYSRMTILALQAAAELEKEGSSCEVVDLRTLA
PLDTETFVASVQKTGRAVVVEECWRAAGLGGHLASIIAEECFDRLLAPVRRVSGLDVPMPYSRKIEKLCIPQVETIAAAV
RDVMSQKY

Sequences:

>Translated_328_residues
MHEMTYRDALNKALKEEMRRDPSVVAWGEDVALYEGSFKVTRGLLAEFGEERVKDTPISENTIIGVSIGAAMGGLRPVAE
LMTVNFALLAMDQIVNHMAKISSMFGGQTHLPMVVRAPGGGGSQLGAQHSQSLETYFMHCPGIHVAVPATPADARGLLKA
SIRDNNPVMFLEHELLYNSKGEVPDDPEFLVPLGKAEVKREGKDVTIVAYSRMTILALQAAAELEKEGSSCEVVDLRTLA
PLDTETFVASVQKTGRAVVVEECWRAAGLGGHLASIIAEECFDRLLAPVRRVSGLDVPMPYSRKIEKLCIPQVETIAAAV
RDVMSQKY
>Mature_328_residues
MHEMTYRDALNKALKEEMRRDPSVVAWGEDVALYEGSFKVTRGLLAEFGEERVKDTPISENTIIGVSIGAAMGGLRPVAE
LMTVNFALLAMDQIVNHMAKISSMFGGQTHLPMVVRAPGGGGSQLGAQHSQSLETYFMHCPGIHVAVPATPADARGLLKA
SIRDNNPVMFLEHELLYNSKGEVPDDPEFLVPLGKAEVKREGKDVTIVAYSRMTILALQAAAELEKEGSSCEVVDLRTLA
PLDTETFVASVQKTGRAVVVEECWRAAGLGGHLASIIAEECFDRLLAPVRRVSGLDVPMPYSRKIEKLCIPQVETIAAAV
RDVMSQKY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=327, Percent_Identity=45.565749235474, Blast_Score=309, Evalue=3e-84,
Organism=Homo sapiens, GI291084858, Length=327, Percent_Identity=43.7308868501529, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=38.5321100917431, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=38.5321100917431, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=46.319018404908, Blast_Score=313, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17506935, Length=321, Percent_Identity=37.6947040498442, Blast_Score=193, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=46.177370030581, Blast_Score=305, Evalue=6e-84,
Organism=Drosophila melanogaster, GI21358145, Length=329, Percent_Identity=48.0243161094225, Blast_Score=315, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24650940, Length=329, Percent_Identity=48.0243161094225, Blast_Score=315, Evalue=2e-86,
Organism=Drosophila melanogaster, GI160714832, Length=325, Percent_Identity=35.6923076923077, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=35.5828220858896, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=53.8461538461538, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=53.8461538461538, Blast_Score=107, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35662; Mature: 35662

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEMTYRDALNKALKEEMRRDPSVVAWGEDVALYEGSFKVTRGLLAEFGEERVKDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
NTIIGVSIGAAMGGLRPVAELMTVNFALLAMDQIVNHMAKISSMFGGQTHLPMVVRAPGG
CEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
GGSQLGAQHSQSLETYFMHCPGIHVAVPATPADARGLLKASIRDNNPVMFLEHELLYNSK
CCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCHHHHEEEECCCCCCEEEEEHHHHCCCC
GEVPDDPEFLVPLGKAEVKREGKDVTIVAYSRMTILALQAAAELEKEGSSCEVVDLRTLA
CCCCCCCCEEEECCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEHHCC
PLDTETFVASVQKTGRAVVVEECWRAAGLGGHLASIIAEECFDRLLAPVRRVSGLDVPMP
CCCHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSRKIEKLCIPQVETIAAAVRDVMSQKY
HHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHEMTYRDALNKALKEEMRRDPSVVAWGEDVALYEGSFKVTRGLLAEFGEERVKDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
NTIIGVSIGAAMGGLRPVAELMTVNFALLAMDQIVNHMAKISSMFGGQTHLPMVVRAPGG
CEEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
GGSQLGAQHSQSLETYFMHCPGIHVAVPATPADARGLLKASIRDNNPVMFLEHELLYNSK
CCCCCCCHHHHHHHHHHEECCCCEEEECCCCCCHHHHEEEECCCCCCEEEEEHHHHCCCC
GEVPDDPEFLVPLGKAEVKREGKDVTIVAYSRMTILALQAAAELEKEGSSCEVVDLRTLA
CCCCCCCCEEEECCHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEHHCC
PLDTETFVASVQKTGRAVVVEECWRAAGLGGHLASIIAEECFDRLLAPVRRVSGLDVPMP
CCCHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSRKIEKLCIPQVETIAAAVRDVMSQKY
HHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]