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The map label for this gene is pdhC [H]

Identifier: 78223948

GI number: 78223948

Start: 3111446

End: 3112741

Strand: Reverse

Name: pdhC [H]

Synonym: Gmet_2752

Alternate gene names: 78223948

Gene position: 3112741-3111446 (Counterclockwise)

Preceding gene: 78223949

Following gene: 78223947

Centisome position: 77.87

GC content: 67.82

Gene sequence:

>1296_bases
ATGCCAACCGATATCACCATGCCCAAACTCTCGGATACCATGACCGAAGGCCGGCTCGTTTCCTGGAAGAAGAGCGTGGG
CGAGCGAGTGGAGCGGGGCGAGATCATCGCCGAGGTGGAGACCGACAAGGCCACCATGGAGCTGGAGGCGTTCGCCTCGG
GAACCCTCGCCGAGCAGCGGGTGAAACCGGGGGAGCTGGTGGCCGTGGGGACGGTGATCGGCGTCATCGGCGCGGGCGGG
GAGATTCCCCCAGTCGCTCCGGAGAAGCCGACGCCATCGCCGGAGGAGCCCAAGCCATCGCCGGAGGAGTCGAAGCCGTC
GCCGCAGAAAGCCGAACCGCAGCCGACGCCGGAAGCAACCCCTGCCGCTCCGGCGGGTGACGTGCCGGAGCGGGTCATGG
AGCTTCCCGAGGAGAAGGCGTCCGCACCGGCCCCGCCGGAAGCGGAACGCGGCGAGGGTGAGCGGGCTGCCCCCGTGGTT
AGGCGGATGGCGCGGGAGCGGGGGATCGACCTAAGCCTCGTGACCGGCAGCGGCCCCGAGGGGAGGATTCTCCAGGAGGA
TCTGGAGCGGTACCTGACGGAGAAGCCAGCACCGGAGAGTGCTGTCGCCACCGGCGAGGGTGCAGAGGGCGAACCCCTGT
CGCGGATGCGGGGCGCCATTGCCCGCGTCACCAGCCAGGCGTGGCAGACCATCCCCCACTTCTACGAGACGGTGGAGATC
GCCATGGAGGAGGGGGTTGAGATCGTCCGGGAGCTGAAGGGGAGCGGGAACGAGGTCACCTTTAACGATCTCGTGGTCAA
GGCGGCGGCCATGGCCCTGGCAAAGTATCCGAGGCTGAACGCATCCTTCGCCGGCGACCGGATCGTCACCCACTGCGAGG
TGAACATCGGGATCGCCGTGGCGGTGGACGACGGCCTCCTGGTGCCGGTGGTGAAGGGGTGCCAGGGGCTCGCTCTGAAG
GAGATTGCCCTGGAGACGGTGCGCCTCGCGGACCGGGCCCGCAGCGGCAGGATCAGCCAGGAGGAGATATCCGGCGGTAC
GTTCAGCATCTCGAACCTGGGGATGTTCGGCATCGATGAGTTCGCAGCGGTGATCTTCCCGCCCCAGGCCGCAATCCTGG
CCGTGGGGAACGTGGCTGACCGCCCGGTGGTCCGGGACGGCCGGGTGGTGGCGGCGAAGACCATGCGGGTGACCCTCTCC
TGTGACCACCGGATCGTTGACGGCGCCTACGCGGCCCGGTTCCTCGGGGAGTTCCGGCGCATCGTTGAAAAACCTGTCCT
GATGTTGTTGCAATAA

Upstream 100 bases:

>100_bases
TACTCGCGCAAGATCGAGAAGCTCTGCATCCCGCAGGTGGAGACCATCGCCGCAGCGGTTCGGGACGTAATGAGTCAGAA
ATACTGACAAGGAGGCACGG

Downstream 100 bases:

>100_bases
TTGCAACTTTATGGCATTTAGTTTGCATCGCTTAGTGTAGTCCACGATGAACGGGGTGAACATGGCGGCGCCGATGCCTG
ATATGACCGGAGAGGGGACG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPTDITMPKLSDTMTEGRLVSWKKSVGERVERGEIIAEVETDKATMELEAFASGTLAEQRVKPGELVAVGTVIGVIGAGG
EIPPVAPEKPTPSPEEPKPSPEESKPSPQKAEPQPTPEATPAAPAGDVPERVMELPEEKASAPAPPEAERGEGERAAPVV
RRMARERGIDLSLVTGSGPEGRILQEDLERYLTEKPAPESAVATGEGAEGEPLSRMRGAIARVTSQAWQTIPHFYETVEI
AMEEGVEIVRELKGSGNEVTFNDLVVKAAAMALAKYPRLNASFAGDRIVTHCEVNIGIAVAVDDGLLVPVVKGCQGLALK
EIALETVRLADRARSGRISQEEISGGTFSISNLGMFGIDEFAAVIFPPQAAILAVGNVADRPVVRDGRVVAAKTMRVTLS
CDHRIVDGAYAARFLGEFRRIVEKPVLMLLQ

Sequences:

>Translated_431_residues
MPTDITMPKLSDTMTEGRLVSWKKSVGERVERGEIIAEVETDKATMELEAFASGTLAEQRVKPGELVAVGTVIGVIGAGG
EIPPVAPEKPTPSPEEPKPSPEESKPSPQKAEPQPTPEATPAAPAGDVPERVMELPEEKASAPAPPEAERGEGERAAPVV
RRMARERGIDLSLVTGSGPEGRILQEDLERYLTEKPAPESAVATGEGAEGEPLSRMRGAIARVTSQAWQTIPHFYETVEI
AMEEGVEIVRELKGSGNEVTFNDLVVKAAAMALAKYPRLNASFAGDRIVTHCEVNIGIAVAVDDGLLVPVVKGCQGLALK
EIALETVRLADRARSGRISQEEISGGTFSISNLGMFGIDEFAAVIFPPQAAILAVGNVADRPVVRDGRVVAAKTMRVTLS
CDHRIVDGAYAARFLGEFRRIVEKPVLMLLQ
>Mature_430_residues
PTDITMPKLSDTMTEGRLVSWKKSVGERVERGEIIAEVETDKATMELEAFASGTLAEQRVKPGELVAVGTVIGVIGAGGE
IPPVAPEKPTPSPEEPKPSPEESKPSPQKAEPQPTPEATPAAPAGDVPERVMELPEEKASAPAPPEAERGEGERAAPVVR
RMARERGIDLSLVTGSGPEGRILQEDLERYLTEKPAPESAVATGEGAEGEPLSRMRGAIARVTSQAWQTIPHFYETVEIA
MEEGVEIVRELKGSGNEVTFNDLVVKAAAMALAKYPRLNASFAGDRIVTHCEVNIGIAVAVDDGLLVPVVKGCQGLALKE
IALETVRLADRARSGRISQEEISGGTFSISNLGMFGIDEFAAVIFPPQAAILAVGNVADRPVVRDGRVVAAKTMRVTLSC
DHRIVDGAYAARFLGEFRRIVEKPVLMLLQ

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=453, Percent_Identity=37.0860927152318, Blast_Score=248, Evalue=7e-66,
Organism=Homo sapiens, GI203098816, Length=475, Percent_Identity=32, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI203098753, Length=474, Percent_Identity=32.4894514767932, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI110671329, Length=459, Percent_Identity=29.6296296296296, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI260898739, Length=265, Percent_Identity=37.3584905660377, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.8017621145374, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786946, Length=446, Percent_Identity=28.47533632287, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1786305, Length=434, Percent_Identity=29.9539170506912, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=442, Percent_Identity=35.972850678733, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17537937, Length=452, Percent_Identity=29.8672566371681, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=442, Percent_Identity=31.2217194570136, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=302, Percent_Identity=31.4569536423841, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324258, Length=463, Percent_Identity=32.829373650108, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=29.1284403669725, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI20129315, Length=287, Percent_Identity=34.4947735191638, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24582497, Length=287, Percent_Identity=34.4947735191638, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=28.8990825688073, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24645909, Length=222, Percent_Identity=34.6846846846847, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 45985; Mature: 45854

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDITMPKLSDTMTEGRLVSWKKSVGERVERGEIIAEVETDKATMELEAFASGTLAEQR
CCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHHEEHHHHHCCCHHHHH
VKPGELVAVGTVIGVIGAGGEIPPVAPEKPTPSPEEPKPSPEESKPSPQKAEPQPTPEAT
CCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAPAGDVPERVMELPEEKASAPAPPEAERGEGERAAPVVRRMARERGIDLSLVTGSGPE
CCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GRILQEDLERYLTEKPAPESAVATGEGAEGEPLSRMRGAIARVTSQAWQTIPHFYETVEI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMEEGVEIVRELKGSGNEVTFNDLVVKAAAMALAKYPRLNASFAGDRIVTHCEVNIGIAV
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEE
AVDDGLLVPVVKGCQGLALKEIALETVRLADRARSGRISQEEISGGTFSISNLGMFGIDE
EECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCHHH
FAAVIFPPQAAILAVGNVADRPVVRDGRVVAAKTMRVTLSCDHRIVDGAYAARFLGEFRR
HHHEEECCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
IVEKPVLMLLQ
HHHHHHHHHCC
>Mature Secondary Structure 
PTDITMPKLSDTMTEGRLVSWKKSVGERVERGEIIAEVETDKATMELEAFASGTLAEQR
CCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCHHEEHHHHHCCCHHHHH
VKPGELVAVGTVIGVIGAGGEIPPVAPEKPTPSPEEPKPSPEESKPSPQKAEPQPTPEAT
CCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PAAPAGDVPERVMELPEEKASAPAPPEAERGEGERAAPVVRRMARERGIDLSLVTGSGPE
CCCCCCCHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
GRILQEDLERYLTEKPAPESAVATGEGAEGEPLSRMRGAIARVTSQAWQTIPHFYETVEI
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMEEGVEIVRELKGSGNEVTFNDLVVKAAAMALAKYPRLNASFAGDRIVTHCEVNIGIAV
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEE
AVDDGLLVPVVKGCQGLALKEIALETVRLADRARSGRISQEEISGGTFSISNLGMFGIDE
EECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCHHH
FAAVIFPPQAAILAVGNVADRPVVRDGRVVAAKTMRVTLSCDHRIVDGAYAARFLGEFRR
HHHEEECCCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
IVEKPVLMLLQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]