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The map label for this gene is yoaE [H]

Identifier: 78223934

GI number: 78223934

Start: 3095747

End: 3097762

Strand: Direct

Name: yoaE [H]

Synonym: Gmet_2738

Alternate gene names: 78223934

Gene position: 3095747-3097762 (Clockwise)

Preceding gene: 78223933

Following gene: 78223935

Centisome position: 77.44

GC content: 67.91

Gene sequence:

>2016_bases
ATGCCCACTGAAATACGACGCTCCGTCTGCCCCTACGACTGCCCCGACACCTGCGGCCTCCTGGTGGAGGTGGAGGACGG
CCGGGCCATGCGCGTTTCCGGCGACCCGGAGCACCCCTTCACCCGGGGCACCCTCTGCCCCAAGATGCTCCACTATGAGA
AGACGGCCCACTCCCCCCTGCGCCTCACAACCCCCCTCGTCCGCATCGGCCCCAAGGGGAAGGGGGAGTTCCGCGCCGCC
ACCTGGAACGAGGCTGTTGCCATAATCGCGGCCCGCTGGCGGGAGATTATCTCCGGCTACGGGGCCGAGGCAATCCTCCC
CTACTCCTACGCCGGAACCATGGGGCTCGTGCACCGCAACTGCGGCCACGCGTTCTTCCACCGGCTGGGGGCGTCACGGC
TCGACAGGACCATCTGCTCGCCGGCCAAGGAGGCGGGGTGGAAGGCAATCATGGGGGATACGCCGTCCCCCCACCCGGAC
GAGGTGGCGGAAAGCGATCTGGTGATCCTCTGGGGGATCAATGCGGCGGCCACCAACATCCACTTCCTCCATGGAGTCAG
GGAGACAAGGAAGCGGGGCGCCCAGGTCTGGCTCATCGACACCTATGAGACCCCCACCGCCGCCGCGGCGGACCGGGTCA
TCCTCTCCCGCCCCGGGAGCGACGGCGCCCTGGCCCTGGGGATGATGCACGTCATCGCCCGGGAAGGGCTCATCGACAAG
GCCTTCGTGGAGGAGCGGATCCAGGGCTTTGCCGAACTGGCGGAGCAGATCCTCCCCGGCTACCCGCCGGAGCGGGTGAG
CGCCATCACCGGTGTTCCCGCTTCGGTCATCGAAGATATGGCGCGCTGCTACGCCCGGGCGCAAGCCCCCTTCATCCGGC
TGGGGAGCGGGCTCTCCCGCTACGGCAACGGCGCCATGACGGTCCGCACCATCTGTGCGCTGCCGGCCCTGGTGGGGGCC
TACGGAAAGCGGGGGGGAGGGCTCCTGGCCTCCACCAGCACCGGCGCCGCCTTTGCCATGAAGGAGGTGACCCGGGAGGA
CTTCATGCTGCGCCCCACCCGGATCGTGAACATGAACCGCCTGGGGCACGCCCTGACGGAGCTGTCCGACCCGCCAGTGA
AGTCCCTCTACGTCTACCACTCAAACCCGGCATCGGTGACCCCGGACCAGAACGCGATCCTTCGAGGACTTGCCCGCAAC
GACCTCTTCACCGTGGTGCACGATCGGTTCATGACCGACACGGCCCGCTGGGCCGACGTGGTGCTCCCCGCCTGCTCGTC
CCTGGAGACGGGGGATGTCTACCGGGCCTACGGCACCTACTGCATCCAGCGTGCATGGCCGGCCATCCCGCCAGTGGGGG
AGAGCAGGTCCAACTGGGACACCTTCGCGTTTCTGGCCGAGGCCATGGGGTTCGACGAACCGTACTTCAGCCAGACCGAG
GAGGAGATCATCGAGCACCTTGTAGCGATCCCCGCCCCCATGAGGGATGGGATCGACCTGGCCCGCTTCGAGGAAGGGAA
GGGGGTGGAGCTTCCCCTGGCCCCGGACGCCCCCCGCACCTTCCGGACCCCGTCGGGGAAGGTGGAAATCCTCAACCACC
GGGAGCCGGAACCCCTTCCCCGGCATCTCCCCACCCACGAGGAAGAGGGGAATCTCCCCCTCATGCTCATGACCGCGCCG
ACCCTCTACGCCCTCAACGCCTCCTTCTACGAGCAGGATGAACTACGGGAAAGACAGGGGGGGATGCGCCTCATGGTGAA
CCCGGTCGAGGCCGGGACGCGGGGAATCGGGGACGGCGAGCGGGTGATCGCCTGCAACGAACGGGGCAAAGTGGAGTTCA
CCCTTGCGGTGACCGACCGGGTCCCTCCCGGCGTGGCCGTGGCCGAGGGAGTCTGGTGGTCGGAGCACGCCCCCGGCGAC
CGGACCGTGAACGCCCTCACCAGCCAGCGCCTCACCGACCGGGGGAACGGGAGCACGTTCTATGACACGAGGATAGAGGT
GAAGAAAGCGAGATAG

Upstream 100 bases:

>100_bases
CCGATTTTGCTCCGTGTCCTCCGTCTCTCCGTGTTATATTTTTTTTGAATTATTTCCTCTTTACGACAACTATTCCATCC
CTTGCAATCGTGAGGTTCCC

Downstream 100 bases:

>100_bases
CAAGTAGCGAAGTAGCAAGTAGTAAGAAACAATAACTATCTCGTTATCTCGCTATTTTACTAAAGTTCCCTCAATGCCAG
CCGATACGAGTATCAGGCCT

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MPTEIRRSVCPYDCPDTCGLLVEVEDGRAMRVSGDPEHPFTRGTLCPKMLHYEKTAHSPLRLTTPLVRIGPKGKGEFRAA
TWNEAVAIIAARWREIISGYGAEAILPYSYAGTMGLVHRNCGHAFFHRLGASRLDRTICSPAKEAGWKAIMGDTPSPHPD
EVAESDLVILWGINAAATNIHFLHGVRETRKRGAQVWLIDTYETPTAAAADRVILSRPGSDGALALGMMHVIAREGLIDK
AFVEERIQGFAELAEQILPGYPPERVSAITGVPASVIEDMARCYARAQAPFIRLGSGLSRYGNGAMTVRTICALPALVGA
YGKRGGGLLASTSTGAAFAMKEVTREDFMLRPTRIVNMNRLGHALTELSDPPVKSLYVYHSNPASVTPDQNAILRGLARN
DLFTVVHDRFMTDTARWADVVLPACSSLETGDVYRAYGTYCIQRAWPAIPPVGESRSNWDTFAFLAEAMGFDEPYFSQTE
EEIIEHLVAIPAPMRDGIDLARFEEGKGVELPLAPDAPRTFRTPSGKVEILNHREPEPLPRHLPTHEEEGNLPLMLMTAP
TLYALNASFYEQDELRERQGGMRLMVNPVEAGTRGIGDGERVIACNERGKVEFTLAVTDRVPPGVAVAEGVWWSEHAPGD
RTVNALTSQRLTDRGNGSTFYDTRIEVKKAR

Sequences:

>Translated_671_residues
MPTEIRRSVCPYDCPDTCGLLVEVEDGRAMRVSGDPEHPFTRGTLCPKMLHYEKTAHSPLRLTTPLVRIGPKGKGEFRAA
TWNEAVAIIAARWREIISGYGAEAILPYSYAGTMGLVHRNCGHAFFHRLGASRLDRTICSPAKEAGWKAIMGDTPSPHPD
EVAESDLVILWGINAAATNIHFLHGVRETRKRGAQVWLIDTYETPTAAAADRVILSRPGSDGALALGMMHVIAREGLIDK
AFVEERIQGFAELAEQILPGYPPERVSAITGVPASVIEDMARCYARAQAPFIRLGSGLSRYGNGAMTVRTICALPALVGA
YGKRGGGLLASTSTGAAFAMKEVTREDFMLRPTRIVNMNRLGHALTELSDPPVKSLYVYHSNPASVTPDQNAILRGLARN
DLFTVVHDRFMTDTARWADVVLPACSSLETGDVYRAYGTYCIQRAWPAIPPVGESRSNWDTFAFLAEAMGFDEPYFSQTE
EEIIEHLVAIPAPMRDGIDLARFEEGKGVELPLAPDAPRTFRTPSGKVEILNHREPEPLPRHLPTHEEEGNLPLMLMTAP
TLYALNASFYEQDELRERQGGMRLMVNPVEAGTRGIGDGERVIACNERGKVEFTLAVTDRVPPGVAVAEGVWWSEHAPGD
RTVNALTSQRLTDRGNGSTFYDTRIEVKKAR
>Mature_670_residues
PTEIRRSVCPYDCPDTCGLLVEVEDGRAMRVSGDPEHPFTRGTLCPKMLHYEKTAHSPLRLTTPLVRIGPKGKGEFRAAT
WNEAVAIIAARWREIISGYGAEAILPYSYAGTMGLVHRNCGHAFFHRLGASRLDRTICSPAKEAGWKAIMGDTPSPHPDE
VAESDLVILWGINAAATNIHFLHGVRETRKRGAQVWLIDTYETPTAAAADRVILSRPGSDGALALGMMHVIAREGLIDKA
FVEERIQGFAELAEQILPGYPPERVSAITGVPASVIEDMARCYARAQAPFIRLGSGLSRYGNGAMTVRTICALPALVGAY
GKRGGGLLASTSTGAAFAMKEVTREDFMLRPTRIVNMNRLGHALTELSDPPVKSLYVYHSNPASVTPDQNAILRGLARND
LFTVVHDRFMTDTARWADVVLPACSSLETGDVYRAYGTYCIQRAWPAIPPVGESRSNWDTFAFLAEAMGFDEPYFSQTEE
EIIEHLVAIPAPMRDGIDLARFEEGKGVELPLAPDAPRTFRTPSGKVEILNHREPEPLPRHLPTHEEEGNLPLMLMTAPT
LYALNASFYEQDELRERQGGMRLMVNPVEAGTRGIGDGERVIACNERGKVEFTLAVTDRVPPGVAVAEGVWWSEHAPGDR
TVNALTSQRLTDRGNGSTFYDTRIEVKKAR

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=776, Percent_Identity=27.9639175257732, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787870, Length=772, Percent_Identity=25.7772020725389, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI3868721, Length=672, Percent_Identity=27.827380952381, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI171474008, Length=756, Percent_Identity=26.5873015873016, Blast_Score=169, Evalue=8e-43,
Organism=Escherichia coli, GI87081994, Length=724, Percent_Identity=24.171270718232, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787231, Length=739, Percent_Identity=23.2746955345061, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1788534, Length=494, Percent_Identity=22.4696356275304, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 73637; Mature: 73506

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00490 MOLYBDOPTERIN_PROK_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEIRRSVCPYDCPDTCGLLVEVEDGRAMRVSGDPEHPFTRGTLCPKMLHYEKTAHSPL
CCCHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCE
RLTTPLVRIGPKGKGEFRAATWNEAVAIIAARWREIISGYGAEAILPYSYAGTMGLVHRN
EECCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
CGHAFFHRLGASRLDRTICSPAKEAGWKAIMGDTPSPHPDEVAESDLVILWGINAAATNI
HHHHHHHHHCHHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHCCCCEEEEEECCHHHHHH
HFLHGVRETRKRGAQVWLIDTYETPTAAAADRVILSRPGSDGALALGMMHVIAREGLIDK
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCHHH
AFVEERIQGFAELAEQILPGYPPERVSAITGVPASVIEDMARCYARAQAPFIRLGSGLSR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEECCCCHHH
YGNGAMTVRTICALPALVGAYGKRGGGLLASTSTGAAFAMKEVTREDFMLRPTRIVNMNR
HCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEECCHHEECHHH
LGHALTELSDPPVKSLYVYHSNPASVTPDQNAILRGLARNDLFTVVHDRFMTDTARWADV
HHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VLPACSSLETGDVYRAYGTYCIQRAWPAIPPVGESRSNWDTFAFLAEAMGFDEPYFSQTE
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
EEIIEHLVAIPAPMRDGIDLARFEEGKGVELPLAPDAPRTFRTPSGKVEILNHREPEPLP
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCC
RHLPTHEEEGNLPLMLMTAPTLYALNASFYEQDELRERQGGMRLMVNPVEAGTRGIGDGE
CCCCCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
RVIACNERGKVEFTLAVTDRVPPGVAVAEGVWWSEHAPGDRTVNALTSQRLTDRGNGSTF
EEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
YDTRIEVKKAR
EEEEEEEEECC
>Mature Secondary Structure 
PTEIRRSVCPYDCPDTCGLLVEVEDGRAMRVSGDPEHPFTRGTLCPKMLHYEKTAHSPL
CCHHHHCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCE
RLTTPLVRIGPKGKGEFRAATWNEAVAIIAARWREIISGYGAEAILPYSYAGTMGLVHRN
EECCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHH
CGHAFFHRLGASRLDRTICSPAKEAGWKAIMGDTPSPHPDEVAESDLVILWGINAAATNI
HHHHHHHHHCHHHHHHHHHCCHHHCCCEEEECCCCCCCHHHHCCCCEEEEEECCHHHHHH
HFLHGVRETRKRGAQVWLIDTYETPTAAAADRVILSRPGSDGALALGMMHVIAREGLIDK
HHHHHHHHHHHCCCEEEEEECCCCCCHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCHHH
AFVEERIQGFAELAEQILPGYPPERVSAITGVPASVIEDMARCYARAQAPFIRLGSGLSR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEECCCCHHH
YGNGAMTVRTICALPALVGAYGKRGGGLLASTSTGAAFAMKEVTREDFMLRPTRIVNMNR
HCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHEECCHHEECHHH
LGHALTELSDPPVKSLYVYHSNPASVTPDQNAILRGLARNDLFTVVHDRFMTDTARWADV
HHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VLPACSSLETGDVYRAYGTYCIQRAWPAIPPVGESRSNWDTFAFLAEAMGFDEPYFSQTE
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
EEIIEHLVAIPAPMRDGIDLARFEEGKGVELPLAPDAPRTFRTPSGKVEILNHREPEPLP
HHHHHHHHHCCCCCCCCCCHHHCCCCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCC
RHLPTHEEEGNLPLMLMTAPTLYALNASFYEQDELRERQGGMRLMVNPVEAGTRGIGDGE
CCCCCCCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
RVIACNERGKVEFTLAVTDRVPPGVAVAEGVWWSEHAPGDRTVNALTSQRLTDRGNGSTF
EEEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
YDTRIEVKKAR
EEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]