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The map label for this gene is pcrA [H]

Identifier: 78223920

GI number: 78223920

Start: 3077250

End: 3079286

Strand: Reverse

Name: pcrA [H]

Synonym: Gmet_2724

Alternate gene names: 78223920

Gene position: 3079286-3077250 (Counterclockwise)

Preceding gene: 78223921

Following gene: 78223918

Centisome position: 77.03

GC content: 64.21

Gene sequence:

>2037_bases
ATGCTTGACCTCTCCACCCTCAATCCACCCCAGCGGGCCGCTGCGGAGCACACCGAGGGGCCGCTCCTGGTCCTGGCCGG
CGCCGGCTCCGGCAAGACCCGGGTCATCACGTACCGGATCGGCCACCTCCTTCTCGACAAGAAGGTGAAGGCCGAGGACA
TCCTGGCGGTCACCTTCACCAACAAGGCGGCAGGCGAGATGAAGGAGCGGGTCCGCGAGCTGGTTGGGCGTGGCAAGGCC
AAGGGGATGGTCATCTCCACCTTCCACTCACTGGGGGTCCGAATCCTGCGCCGGGACATCGAACGGCTCGGTTACAAGAA
GAATTTCAGCATCTACACCACCTCGGACCAGATCGGCCTCATCCGCCAGGCCATGCGGGAGGTGAACGCCGACGGGAAGA
AGTTCGACGCCGAGACGCTCCTCTGGCGGATCTCCGGCTGGAAAAACGGACTCATCCCGCCGGACCGGGCCGTCCCCCGC
TACGACGATCCCGACGAGCTCCTGGCCTGCGACGTCTACCCTCGCTACCAGGCGCTCCTCCACGCCTGCAACGCCATCGA
CTTCGACGACATCATCATGCTGACGGTGCGGCTCCTCCAGGACTACCCTGAGGTGCTGGACCACTGGCAGGAGCGCTTCC
GCTATATCATGGTGGACGAGTACCAGGACACCAACGCCTCCCAGTATCTCTTCATTTCGCTCCTGGCGCGGAAGTACCGC
AACCTCTGCGTGGTGGGGGACGATGATCAGTCCATCTACGGCTGGCGCGGGGCCGACGTGGGGAACATCCTCGACTTCGA
GCGGGACTACCCGGGGTGCCGGGTGGTGAAGCTGGAGCAGAACTACCGCTCCACCGGCACGATCTTGGAGGCGGCGAACC
AGGTCATCAAGAACAACGCGAAACGGAAGGAGAAGCGTCTCTGGACCGATGTGGGGGAGGGACGCCCCATCGACATGGTC
ATCGCCCAGGACGACGAGGAGGAGGCGACCCTGGTGGTGGAGCGGCTCCAGATGGAGCGCTACCAGCATAACCTCTCCTT
CGGCGACTTCGCCATCCTCTACCGGACCAACTCCCAGAGCCGGGCCTTCGAGGAGCAGCTCCGCTTCGAGGACATCCCCT
ACGTGCTCGTGGGGGGGATGCAGTTCTACGAGCGCAAGGAGGTTAAGGACGCCCTCTCGTACCTGAAGGTGATCGACAAC
CCCCTGGACGAGCAGGCCCTGTTGCGGGTGGTGAATTTCCCCCGCCGGGGGATCGGCGACGGGACCGTGGTGAAGATCAA
CCAGTGGTCCCTGGAGCACGAATGCCCGCTCTTGGAGGCTATGGGGAGGGTGGAGGAGATCGAAGGCATCCCTGGCGCGA
CCCGGGAGAAGGTCCTGACCTTCCACCGGCTCATCACGGGCGCGGCCGGGGAGTTCCGCCGTCCGGGAGATCTGGCGGCG
CGGGTGAAGGGGTTCTACGACCGGATCGGCCTTGAGGAGGAGCTCTACCGTACCATCGAGGAGCCGGCCACGGCCCGGCG
CAAGGTGGAGAACGTGGAGCAGATCATCAACTCCATGGCGGGGTACGAGGAGAGGATTCCGGGGGCGACCCTCTCGGGGT
TCCTGGAGAAGGTCTCCCTCATGGACGAGGATCGCTTCAGCGGCAAGGACAAGAAGGAGCATGGCAAGGATGCCGTGACG
CTCATGTCGCTCCACTCCAGCAAGGGGCTGGAGTTCCCCTTCGTTTTTTTAGTGGGGTTCGAGGAGGAGATCCTCCCCCA
CAAGAAGTCCGTGGAGGAGGGGATGAGCATCGACGAGGAGCGGCGCCTCTGCTACGTGGGGATCACCCGGGCCCGCCGGC
ACCTCACCATAACCCGCTGCCTCCACCGGAAGAAGTACGGCAAGCTCCTGGAGCGGCAGCCGAGCCGCTTCCTGGAGGAG
ATTCCGGAGCGGCTCGTGCAAGTTCATGAAGGTGAGGTGAAGAAGGCCGCCACCGAGGAGGAGCAGGAGGCCATGGCCCA
GGATTTCTTCGCCCGGATGCGGGAGAAACTTTCCTGA

Upstream 100 bases:

>100_bases
TTTCTCCCGACACCTCTGCTATCAATGATGACAGCCGGGCCGACCAGCCCGGCTGTCGTGCGTCTGGAGCACCGATTCAA
CCGTTTCGAGGATTATTTTT

Downstream 100 bases:

>100_bases
AAAAGTTAGTTTGAATAACACGGAGAAACGGAGAGCACGGAGAAAATCAATAACGTTAATGGATTTGGTTGGGGTCTCAG
CAGGTGATGCCAATCAGATG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 678

Protein sequence:

>678_residues
MLDLSTLNPPQRAAAEHTEGPLLVLAGAGSGKTRVITYRIGHLLLDKKVKAEDILAVTFTNKAAGEMKERVRELVGRGKA
KGMVISTFHSLGVRILRRDIERLGYKKNFSIYTTSDQIGLIRQAMREVNADGKKFDAETLLWRISGWKNGLIPPDRAVPR
YDDPDELLACDVYPRYQALLHACNAIDFDDIIMLTVRLLQDYPEVLDHWQERFRYIMVDEYQDTNASQYLFISLLARKYR
NLCVVGDDDQSIYGWRGADVGNILDFERDYPGCRVVKLEQNYRSTGTILEAANQVIKNNAKRKEKRLWTDVGEGRPIDMV
IAQDDEEEATLVVERLQMERYQHNLSFGDFAILYRTNSQSRAFEEQLRFEDIPYVLVGGMQFYERKEVKDALSYLKVIDN
PLDEQALLRVVNFPRRGIGDGTVVKINQWSLEHECPLLEAMGRVEEIEGIPGATREKVLTFHRLITGAAGEFRRPGDLAA
RVKGFYDRIGLEEELYRTIEEPATARRKVENVEQIINSMAGYEERIPGATLSGFLEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGFEEEILPHKKSVEEGMSIDEERRLCYVGITRARRHLTITRCLHRKKYGKLLERQPSRFLEE
IPERLVQVHEGEVKKAATEEEQEAMAQDFFARMREKLS

Sequences:

>Translated_678_residues
MLDLSTLNPPQRAAAEHTEGPLLVLAGAGSGKTRVITYRIGHLLLDKKVKAEDILAVTFTNKAAGEMKERVRELVGRGKA
KGMVISTFHSLGVRILRRDIERLGYKKNFSIYTTSDQIGLIRQAMREVNADGKKFDAETLLWRISGWKNGLIPPDRAVPR
YDDPDELLACDVYPRYQALLHACNAIDFDDIIMLTVRLLQDYPEVLDHWQERFRYIMVDEYQDTNASQYLFISLLARKYR
NLCVVGDDDQSIYGWRGADVGNILDFERDYPGCRVVKLEQNYRSTGTILEAANQVIKNNAKRKEKRLWTDVGEGRPIDMV
IAQDDEEEATLVVERLQMERYQHNLSFGDFAILYRTNSQSRAFEEQLRFEDIPYVLVGGMQFYERKEVKDALSYLKVIDN
PLDEQALLRVVNFPRRGIGDGTVVKINQWSLEHECPLLEAMGRVEEIEGIPGATREKVLTFHRLITGAAGEFRRPGDLAA
RVKGFYDRIGLEEELYRTIEEPATARRKVENVEQIINSMAGYEERIPGATLSGFLEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGFEEEILPHKKSVEEGMSIDEERRLCYVGITRARRHLTITRCLHRKKYGKLLERQPSRFLEE
IPERLVQVHEGEVKKAATEEEQEAMAQDFFARMREKLS
>Mature_678_residues
MLDLSTLNPPQRAAAEHTEGPLLVLAGAGSGKTRVITYRIGHLLLDKKVKAEDILAVTFTNKAAGEMKERVRELVGRGKA
KGMVISTFHSLGVRILRRDIERLGYKKNFSIYTTSDQIGLIRQAMREVNADGKKFDAETLLWRISGWKNGLIPPDRAVPR
YDDPDELLACDVYPRYQALLHACNAIDFDDIIMLTVRLLQDYPEVLDHWQERFRYIMVDEYQDTNASQYLFISLLARKYR
NLCVVGDDDQSIYGWRGADVGNILDFERDYPGCRVVKLEQNYRSTGTILEAANQVIKNNAKRKEKRLWTDVGEGRPIDMV
IAQDDEEEATLVVERLQMERYQHNLSFGDFAILYRTNSQSRAFEEQLRFEDIPYVLVGGMQFYERKEVKDALSYLKVIDN
PLDEQALLRVVNFPRRGIGDGTVVKINQWSLEHECPLLEAMGRVEEIEGIPGATREKVLTFHRLITGAAGEFRRPGDLAA
RVKGFYDRIGLEEELYRTIEEPATARRKVENVEQIINSMAGYEERIPGATLSGFLEKVSLMDEDRFSGKDKKEHGKDAVT
LMSLHSSKGLEFPFVFLVGFEEEILPHKKSVEEGMSIDEERRLCYVGITRARRHLTITRCLHRKKYGKLLERQPSRFLEE
IPERLVQVHEGEVKKAATEEEQEAMAQDFFARMREKLS

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=680, Percent_Identity=42.0588235294118, Blast_Score=497, Evalue=1e-141,
Organism=Escherichia coli, GI2367296, Length=665, Percent_Identity=40.1503759398496, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=28.0701754385965, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=751, Percent_Identity=29.560585885486, Blast_Score=219, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324477, Length=675, Percent_Identity=22.0740740740741, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77851; Mature: 77851

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSTLNPPQRAAAEHTEGPLLVLAGAGSGKTRVITYRIGHLLLDKKVKAEDILAVTFT
CCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEEEEHHHHHHCCCCCCCCEEEEEEC
NKAAGEMKERVRELVGRGKAKGMVISTFHSLGVRILRRDIERLGYKKNFSIYTTSDQIGL
CHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHH
IRQAMREVNADGKKFDAETLLWRISGWKNGLIPPDRAVPRYDDPDELLACDVYPRYQALL
HHHHHHHHCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCHHHEEEECCCHHHHHHH
HACNAIDFDDIIMLTVRLLQDYPEVLDHWQERFRYIMVDEYQDTNASQYLFISLLARKYR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHC
NLCVVGDDDQSIYGWRGADVGNILDFERDYPGCRVVKLEQNYRSTGTILEAANQVIKNNA
CEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCH
KRKEKRLWTDVGEGRPIDMVIAQDDEEEATLVVERLQMERYQHNLSFGDFAILYRTNSQS
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
RAFEEQLRFEDIPYVLVGGMQFYERKEVKDALSYLKVIDNPLDEQALLRVVNFPRRGIGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GTVVKINQWSLEHECPLLEAMGRVEEIEGIPGATREKVLTFHRLITGAAGEFRRPGDLAA
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RVKGFYDRIGLEEELYRTIEEPATARRKVENVEQIINSMAGYEERIPGATLSGFLEKVSL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
MDEDRFSGKDKKEHGKDAVTLMSLHSSKGLEFPFVFLVGFEEEILPHKKSVEEGMSIDEE
HCCCCCCCCCCHHHCCHHEEHEEHHCCCCCCCCEEEEECCHHHHCCCHHHHHHCCCCCHH
RRLCYVGITRARRHLTITRCLHRKKYGKLLERQPSRFLEEIPERLVQVHEGEVKKAATEE
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCHH
EQEAMAQDFFARMREKLS
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLSTLNPPQRAAAEHTEGPLLVLAGAGSGKTRVITYRIGHLLLDKKVKAEDILAVTFT
CCCCCCCCCHHHHHHCCCCCCEEEEEECCCCCEEEEEEEHHHHHHCCCCCCCCEEEEEEC
NKAAGEMKERVRELVGRGKAKGMVISTFHSLGVRILRRDIERLGYKKNFSIYTTSDQIGL
CHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHH
IRQAMREVNADGKKFDAETLLWRISGWKNGLIPPDRAVPRYDDPDELLACDVYPRYQALL
HHHHHHHHCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCCCHHHEEEECCCHHHHHHH
HACNAIDFDDIIMLTVRLLQDYPEVLDHWQERFRYIMVDEYQDTNASQYLFISLLARKYR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHC
NLCVVGDDDQSIYGWRGADVGNILDFERDYPGCRVVKLEQNYRSTGTILEAANQVIKNNA
CEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCH
KRKEKRLWTDVGEGRPIDMVIAQDDEEEATLVVERLQMERYQHNLSFGDFAILYRTNSQS
HHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
RAFEEQLRFEDIPYVLVGGMQFYERKEVKDALSYLKVIDNPLDEQALLRVVNFPRRGIGD
HHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCC
GTVVKINQWSLEHECPLLEAMGRVEEIEGIPGATREKVLTFHRLITGAAGEFRRPGDLAA
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHH
RVKGFYDRIGLEEELYRTIEEPATARRKVENVEQIINSMAGYEERIPGATLSGFLEKVSL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHH
MDEDRFSGKDKKEHGKDAVTLMSLHSSKGLEFPFVFLVGFEEEILPHKKSVEEGMSIDEE
HCCCCCCCCCCHHHCCHHEEHEEHHCCCCCCCCEEEEECCHHHHCCCHHHHHHCCCCCHH
RRLCYVGITRARRHLTITRCLHRKKYGKLLERQPSRFLEEIPERLVQVHEGEVKKAATEE
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHCHH
EQEAMAQDFFARMREKLS
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]