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The map label for this gene is pqqE [H]

Identifier: 78223860

GI number: 78223860

Start: 3014263

End: 3015414

Strand: Reverse

Name: pqqE [H]

Synonym: Gmet_2664

Alternate gene names: 78223860

Gene position: 3015414-3014263 (Counterclockwise)

Preceding gene: 78223861

Following gene: 78223859

Centisome position: 75.43

GC content: 64.06

Gene sequence:

>1152_bases
GTGAACGGCGCCTATACCCTCGAATGCGCGGTTTGGGAGTTTACCCTGGCCTGCAACCTGAACTGCATCCACTGCGGGTC
GAGCGCGGGGAGCAGGAGAACCGACGAGCTGACGACCGACGAGGCCGTGGCCCTGTGTCATGACCTGAAAGAGGCCGGCT
GCCTCGGCGTTGCCCTCATGGGGGGCGAGCCGCTCCTGAGGAAGGATTTCTGGCAGATCGCCGGCCTCATCAGGAAGCTC
GGCATGGAGCTCTCCGTCATCACCAACGGGACGATTCATGACCCCGAGATCTTCGCGCGGCTCAGGGAACTGGACCCCCG
CGCCGTTGCCGTGAGCCTCGATGCCGCAACCCCCGCCATCCACGACCATATCCGGGGGCGGAGCGGCGCCTTCCGGGAAA
CCTGGGATTTCATCCGGGCGGCAGGGGAGGCGGGACTGCCGGTGAGCGTCATCACCACGGTCCACAGGATGAACCTGACG
GAACTTACGGCTCTGCGGGAGCAGTTGCTGAGGAAAAACGTTGCGTGGCAGATCCAGGTGGCCGGGGCCGAGGGAAGGAG
ATTCCCCCGGGAGTTGCTCCTGGACGAGGAAGAGTTCTATTCCGTGGGGCTCTTCATCGCCTCCACCCGAAAAGCGTACC
CGATTCACGCGATGCCGGTCATCGGCGCCCATGACATGGGCTACCACTCCCTGATGCTGGACAACATCTCGTTGACCGGT
GAGTGGACGGGGTGCCAGGCCGGGGTGTCGGTCATCGGCATCCAGAGCAACGGCAACATCAAGGGGTGCCTCTCCATGGA
CGACAGCACCATTGCCGGCAATGTGCGGGAGACGTCGGTCCGCGATCTCTGGCGGAGCGCCGGGGCTTTCCCCTATTCGC
GGGGCTTTACCGCCGACGACGCCGGGGAGAACTGCCGCGTATGCCCGCACTTGGCCACGTGCAAGGGGGGATGCAACGAG
ATGTCCCTCATGAAGACCGGGGAATTCCACAACGATCCCTACTGCTTCCACCGGATCGAGCAGCACATTTTCGAGAGAGA
GCTGCGAAACCCCTGGTACCGGCTCCTGTTGAAGGCGCGCAGGAGGCTCGCGACCCTCGGCGCCCGGGGGAATGAAAAAA
GGCTGAGGGACTCGTTTCTCGGCCACCGGTAG

Upstream 100 bases:

>100_bases
CGACTGTGGCGGTCCCTTCCCCGGTTATCCCTGCGGCACCAAGTACTACACCGTGAGCGTCCGGGATTTCCGGCACCTCT
CCCGCTGAGCGGGGTAGGTC

Downstream 100 bases:

>100_bases
ACCATGAAACTGAACATCGGCTGCGGCCATACCTACCTGAAAGGGCACCTGAACATCGACGTCAGCGGCGATTCTGTCGC
CGACGCCATTATGGAGGCCC

Product: radical SAM family protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MNGAYTLECAVWEFTLACNLNCIHCGSSAGSRRTDELTTDEAVALCHDLKEAGCLGVALMGGEPLLRKDFWQIAGLIRKL
GMELSVITNGTIHDPEIFARLRELDPRAVAVSLDAATPAIHDHIRGRSGAFRETWDFIRAAGEAGLPVSVITTVHRMNLT
ELTALREQLLRKNVAWQIQVAGAEGRRFPRELLLDEEEFYSVGLFIASTRKAYPIHAMPVIGAHDMGYHSLMLDNISLTG
EWTGCQAGVSVIGIQSNGNIKGCLSMDDSTIAGNVRETSVRDLWRSAGAFPYSRGFTADDAGENCRVCPHLATCKGGCNE
MSLMKTGEFHNDPYCFHRIEQHIFERELRNPWYRLLLKARRRLATLGARGNEKRLRDSFLGHR

Sequences:

>Translated_383_residues
MNGAYTLECAVWEFTLACNLNCIHCGSSAGSRRTDELTTDEAVALCHDLKEAGCLGVALMGGEPLLRKDFWQIAGLIRKL
GMELSVITNGTIHDPEIFARLRELDPRAVAVSLDAATPAIHDHIRGRSGAFRETWDFIRAAGEAGLPVSVITTVHRMNLT
ELTALREQLLRKNVAWQIQVAGAEGRRFPRELLLDEEEFYSVGLFIASTRKAYPIHAMPVIGAHDMGYHSLMLDNISLTG
EWTGCQAGVSVIGIQSNGNIKGCLSMDDSTIAGNVRETSVRDLWRSAGAFPYSRGFTADDAGENCRVCPHLATCKGGCNE
MSLMKTGEFHNDPYCFHRIEQHIFERELRNPWYRLLLKARRRLATLGARGNEKRLRDSFLGHR
>Mature_383_residues
MNGAYTLECAVWEFTLACNLNCIHCGSSAGSRRTDELTTDEAVALCHDLKEAGCLGVALMGGEPLLRKDFWQIAGLIRKL
GMELSVITNGTIHDPEIFARLRELDPRAVAVSLDAATPAIHDHIRGRSGAFRETWDFIRAAGEAGLPVSVITTVHRMNLT
ELTALREQLLRKNVAWQIQVAGAEGRRFPRELLLDEEEFYSVGLFIASTRKAYPIHAMPVIGAHDMGYHSLMLDNISLTG
EWTGCQAGVSVIGIQSNGNIKGCLSMDDSTIAGNVRETSVRDLWRSAGAFPYSRGFTADDAGENCRVCPHLATCKGGCNE
MSLMKTGEFHNDPYCFHRIEQHIFERELRNPWYRLLLKARRRLATLGARGNEKRLRDSFLGHR

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42529; Mature: 42529

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGAYTLECAVWEFTLACNLNCIHCGSSAGSRRTDELTTDEAVALCHDLKEAGCLGVALM
CCCCEEEEEEEEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGEPLLRKDFWQIAGLIRKLGMELSVITNGTIHDPEIFARLRELDPRAVAVSLDAATPAI
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHH
HDHIRGRSGAFRETWDFIRAAGEAGLPVSVITTVHRMNLTELTALREQLLRKNVAWQIQV
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE
AGAEGRRFPRELLLDEEEFYSVGLFIASTRKAYPIHAMPVIGAHDMGYHSLMLDNISLTG
ECCCCCCCCHHHHCCCHHHHEEEEEEECCCCCCCEEEECEECCCCCCCCEEEEECEEEEC
EWTGCQAGVSVIGIQSNGNIKGCLSMDDSTIAGNVRETSVRDLWRSAGAFPYSRGFTADD
CCCCCCCCCEEEEEECCCCEEEEEECCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCC
AGENCRVCPHLATCKGGCNEMSLMKTGEFHNDPYCFHRIEQHIFERELRNPWYRLLLKAR
CCCCCEECCCHHHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRLATLGARGNEKRLRDSFLGHR
HHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNGAYTLECAVWEFTLACNLNCIHCGSSAGSRRTDELTTDEAVALCHDLKEAGCLGVALM
CCCCEEEEEEEEEEEEEECCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
GGEPLLRKDFWQIAGLIRKLGMELSVITNGTIHDPEIFARLRELDPRAVAVSLDAATPAI
CCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCHH
HDHIRGRSGAFRETWDFIRAAGEAGLPVSVITTVHRMNLTELTALREQLLRKNVAWQIQV
HHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEE
AGAEGRRFPRELLLDEEEFYSVGLFIASTRKAYPIHAMPVIGAHDMGYHSLMLDNISLTG
ECCCCCCCCHHHHCCCHHHHEEEEEEECCCCCCCEEEECEECCCCCCCCEEEEECEEEEC
EWTGCQAGVSVIGIQSNGNIKGCLSMDDSTIAGNVRETSVRDLWRSAGAFPYSRGFTADD
CCCCCCCCCEEEEEECCCCEEEEEECCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCCCC
AGENCRVCPHLATCKGGCNEMSLMKTGEFHNDPYCFHRIEQHIFERELRNPWYRLLLKAR
CCCCCEECCCHHHCCCCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
RRLATLGARGNEKRLRDSFLGHR
HHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA