Click here to switch to the map view.

The map label for this gene is pckG [H]

Identifier: 78223835

GI number: 78223835

Start: 2985002

End: 2986855

Strand: Direct

Name: pckG [H]

Synonym: Gmet_2638

Alternate gene names: 78223835

Gene position: 2985002-2986855 (Clockwise)

Preceding gene: 78223834

Following gene: 78223841

Centisome position: 74.67

GC content: 62.73

Gene sequence:

>1854_bases
ATGAGCTACGAGTTACCAGCAAACGAACAGCTTTTGAAGTGGGTGGACGAAGTCAGGGCACTCTGTGAACCGTCCCGGAT
TCACTGGTGTGACGGCTCCCAGGAGGAGTACGACGAGCTGTGTAAACTGCTGGTGGAGAATGGCACCTTCAAGAAGCTGA
ACCAGGAAAAGCGCCCCAACAGCTATCTGGCCTGCTCCGATCCCATCGACGTGGCGCGGGTCGAGGACCGCACCTTCATC
TGCAGCATCTCCAAGCAGGATGCAGGGCCCACCAACAACTGGATGGCCCCCAAAGAGATGAAGGAAAAACTCCACGGCCT
GTTCAAGGGATGCATGAAGGGGCGCACCATGTATGTCATCCCCTTCAGCATGGGTCCCCTCGGCTCCCCCATTGCCAAGA
TCGGCGTTGAAATATCGGATTCACCCTACGTTGCCGCCAATATGCGGATCATGACCCGCATGGGCAAAGCCGTTCTCGAT
GTCCTCGGCGGCGGCGAGTTCGTCCCCTGCCTCCACTCGGTGGGCGCCCCCCTTCAGCCTGGACAGAAGGACGTGATCTG
GCCCTGCAACAAGGAGAAATACATCGTCCACTTCCCCGAGGAGCGCGCCATCTGGTCTTTCGGTTCCGGCTACGGCGGCA
ACGCCCTTCTCGGCAAGAAGTGCCTGGCCCTGCGCATCGCCTCCAAGCAGGGGAAGGACGAGGGGTGGCTGGCCGAGCAC
ATGCTGATCCTCGGCGTCCAGTCCCCCACCGGCGAGAAGACCTACGTGGGCGCCGCTTTCCCGAGCGCCTGCGGCAAGAC
CAACTTCGCCATGCTGATTCCCCCGAAGCCGTTGCAGGACAAGGGATGGAAGGTGACCACCCTGGGCGACGACATCGCCT
GGATCAAGCCCGGCCCCGACGGTCAGGTCTATGCCATCAACCCCGAGTACGGCTACTTCGGCGTCGCCCCCGGCACCAAC
GTCAAGACAAACCCCAACGCCATGGCCTCCTGCTCCGCCAATACCATCTTCACCAACGTGGCCCTTACCGAAGACGGCGA
CGTCTGGTGGGAAGGGATGACCGACGAGCCCCCCGCAAAGCTCACCGACTGGCAGGGGAACGAGTGGACCCCGGGGTGCG
GCCGTCTTGCCGCCCATCCCAACTCTCGTTTCACCGCGCCGGCCTCCCAGTGCCCCTCCATCGACCCCGACTGGGAAAAC
CCCAAGGGCGTCCCCATGAGCGCCTTCATCTTCGGTGGCCGCCGCAAGGACACCGTGCCGCTGGTCTACCAGGCCTTCAA
CTGGAGCTTCGGCGTTTACCTCGCCTCCACCCTCGGATCCGAAACCACCGCCGCCGCAGTGGGAGCCACCGGCAACGTGC
GCCGCGACCCCTTCGCCATGCTCCCCTTCTGCGGCTACCACATGGCCGATTACTTCAGCCACTGGCTCCAGTTCGGCCGC
TCCATCGCCAAACCGCCCCGCATCTTCAGCGTCAACTGGTTCCGCAAGGACGCCAACGGCAAGTTTATCTGGCCCGGCTA
CGGCGACAACATGCGGGTTCTCAAGTGGATCGTCGAAAGGGTCCAGGGAAAGGCCGCGGCCGTCGAATCCCCCCTGGGGT
GGATGCCGCGTTACGAGGACATGGACTGGGAAGGGCTCGACATGACCCGCGAGAAGTTCAACGAGCTGACCTCCGTGGAC
CGGGAGGTCTGGCATAACGAGCTCGTCTCCCACGAGGAGCTTTTCCTCAAGCTCTATGACCGCCTCCCCAAGGAGTTCCT
CCATATGCGCCAGATGATCGTTTCCGGCCTCTGGCGCTCGCCGGAGCACTGGGAGCAGATCGGCGACGTCCATCCCGAAG
GGTGGAACGAGTAA

Upstream 100 bases:

>100_bases
TCGCATTGAATGAAATACCGTTAAAGCTTATCATGGCCGAATCCCTTCGCGGAATCGCGAAGGGGCTAACTCATTAACAT
GGCATAAGGAGGCAGTCGGC

Downstream 100 bases:

>100_bases
CGCCCAGTCTCCTGTTACACACAAAGGCCCGTCGGAATTTTCCGGCGGGCCTTTGGTTTTCTGCATGAACGAGCGCTGTG
ACTGTCAGAGGGTAAAAAAG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MSYELPANEQLLKWVDEVRALCEPSRIHWCDGSQEEYDELCKLLVENGTFKKLNQEKRPNSYLACSDPIDVARVEDRTFI
CSISKQDAGPTNNWMAPKEMKEKLHGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEISDSPYVAANMRIMTRMGKAVLD
VLGGGEFVPCLHSVGAPLQPGQKDVIWPCNKEKYIVHFPEERAIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEH
MLILGVQSPTGEKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTLGDDIAWIKPGPDGQVYAINPEYGYFGVAPGTN
VKTNPNAMASCSANTIFTNVALTEDGDVWWEGMTDEPPAKLTDWQGNEWTPGCGRLAAHPNSRFTAPASQCPSIDPDWEN
PKGVPMSAFIFGGRRKDTVPLVYQAFNWSFGVYLASTLGSETTAAAVGATGNVRRDPFAMLPFCGYHMADYFSHWLQFGR
SIAKPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAAAVESPLGWMPRYEDMDWEGLDMTREKFNELTSVD
REVWHNELVSHEELFLKLYDRLPKEFLHMRQMIVSGLWRSPEHWEQIGDVHPEGWNE

Sequences:

>Translated_617_residues
MSYELPANEQLLKWVDEVRALCEPSRIHWCDGSQEEYDELCKLLVENGTFKKLNQEKRPNSYLACSDPIDVARVEDRTFI
CSISKQDAGPTNNWMAPKEMKEKLHGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEISDSPYVAANMRIMTRMGKAVLD
VLGGGEFVPCLHSVGAPLQPGQKDVIWPCNKEKYIVHFPEERAIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEH
MLILGVQSPTGEKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTLGDDIAWIKPGPDGQVYAINPEYGYFGVAPGTN
VKTNPNAMASCSANTIFTNVALTEDGDVWWEGMTDEPPAKLTDWQGNEWTPGCGRLAAHPNSRFTAPASQCPSIDPDWEN
PKGVPMSAFIFGGRRKDTVPLVYQAFNWSFGVYLASTLGSETTAAAVGATGNVRRDPFAMLPFCGYHMADYFSHWLQFGR
SIAKPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAAAVESPLGWMPRYEDMDWEGLDMTREKFNELTSVD
REVWHNELVSHEELFLKLYDRLPKEFLHMRQMIVSGLWRSPEHWEQIGDVHPEGWNE
>Mature_616_residues
SYELPANEQLLKWVDEVRALCEPSRIHWCDGSQEEYDELCKLLVENGTFKKLNQEKRPNSYLACSDPIDVARVEDRTFIC
SISKQDAGPTNNWMAPKEMKEKLHGLFKGCMKGRTMYVIPFSMGPLGSPIAKIGVEISDSPYVAANMRIMTRMGKAVLDV
LGGGEFVPCLHSVGAPLQPGQKDVIWPCNKEKYIVHFPEERAIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEHM
LILGVQSPTGEKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTLGDDIAWIKPGPDGQVYAINPEYGYFGVAPGTNV
KTNPNAMASCSANTIFTNVALTEDGDVWWEGMTDEPPAKLTDWQGNEWTPGCGRLAAHPNSRFTAPASQCPSIDPDWENP
KGVPMSAFIFGGRRKDTVPLVYQAFNWSFGVYLASTLGSETTAAAVGATGNVRRDPFAMLPFCGYHMADYFSHWLQFGRS
IAKPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAAAVESPLGWMPRYEDMDWEGLDMTREKFNELTSVDR
EVWHNELVSHEELFLKLYDRLPKEFLHMRQMIVSGLWRSPEHWEQIGDVHPEGWNE

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=593, Percent_Identity=52.1079258010118, Blast_Score=604, Evalue=1e-173,
Organism=Homo sapiens, GI66346721, Length=590, Percent_Identity=52.2033898305085, Blast_Score=600, Evalue=1e-171,
Organism=Homo sapiens, GI66346723, Length=376, Percent_Identity=55.0531914893617, Blast_Score=400, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17555492, Length=581, Percent_Identity=51.4629948364888, Blast_Score=584, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71989645, Length=579, Percent_Identity=51.6407599309154, Blast_Score=577, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989658, Length=579, Percent_Identity=51.6407599309154, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989653, Length=560, Percent_Identity=52.3214285714286, Blast_Score=570, Evalue=1e-163,
Organism=Caenorhabditis elegans, GI32564411, Length=556, Percent_Identity=52.158273381295, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI32564407, Length=489, Percent_Identity=54.601226993865, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71989649, Length=489, Percent_Identity=53.3742331288344, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI32564409, Length=335, Percent_Identity=51.9402985074627, Blast_Score=348, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17540956, Length=527, Percent_Identity=25.0474383301708, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24655085, Length=568, Percent_Identity=52.4647887323944, Blast_Score=580, Evalue=1e-166,
Organism=Drosophila melanogaster, GI24655082, Length=577, Percent_Identity=50.6065857885615, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI161077226, Length=546, Percent_Identity=51.4652014652015, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24655088, Length=473, Percent_Identity=54.5454545454545, Blast_Score=511, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 69156; Mature: 69024

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYELPANEQLLKWVDEVRALCEPSRIHWCDGSQEEYDELCKLLVENGTFKKLNQEKRPN
CCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCC
SYLACSDPIDVARVEDRTFICSISKQDAGPTNNWMAPKEMKEKLHGLFKGCMKGRTMYVI
CEEEECCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PFSMGPLGSPIAKIGVEISDSPYVAANMRIMTRMGKAVLDVLGGGEFVPCLHSVGAPLQP
EECCCCCCCCHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
GQKDVIWPCNKEKYIVHFPEERAIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEH
CCCCEEECCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHEEEEEECCCCCCCCCHHHEE
MLILGVQSPTGEKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTLGDDIAWIKPGPD
EEEEEECCCCCCCEEEECCCCHHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCC
GQVYAINPEYGYFGVAPGTNVKTNPNAMASCSANTIFTNVALTEDGDVWWEGMTDEPPAK
CCEEEECCCCCEEECCCCCCCCCCCCCCEECCCCEEEEEEEEECCCCEEECCCCCCCCCC
LTDWQGNEWTPGCGRLAAHPNSRFTAPASQCPSIDPDWENPKGVPMSAFIFGGRRKDTVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LVYQAFNWSFGVYLASTLGSETTAAAVGATGNVRRDPFAMLPFCGYHMADYFSHWLQFGR
EEEEECCCCCHHHHHHHCCCCHHHEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHH
SIAKPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAAAVESPLGWMPRYED
HHCCCCCEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
MDWEGLDMTREKFNELTSVDREVWHNELVSHEELFLKLYDRLPKEFLHMRQMIVSGLWRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEHWEQIGDVHPEGWNE
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SYELPANEQLLKWVDEVRALCEPSRIHWCDGSQEEYDELCKLLVENGTFKKLNQEKRPN
CCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHCCCC
SYLACSDPIDVARVEDRTFICSISKQDAGPTNNWMAPKEMKEKLHGLFKGCMKGRTMYVI
CEEEECCCCCCEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEE
PFSMGPLGSPIAKIGVEISDSPYVAANMRIMTRMGKAVLDVLGGGEFVPCLHSVGAPLQP
EECCCCCCCCHHHHCEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
GQKDVIWPCNKEKYIVHFPEERAIWSFGSGYGGNALLGKKCLALRIASKQGKDEGWLAEH
CCCCEEECCCCCCEEEECCCCCCEEECCCCCCCCCHHHHHEEEEEECCCCCCCCCHHHEE
MLILGVQSPTGEKTYVGAAFPSACGKTNFAMLIPPKPLQDKGWKVTTLGDDIAWIKPGPD
EEEEEECCCCCCCEEEECCCCHHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCC
GQVYAINPEYGYFGVAPGTNVKTNPNAMASCSANTIFTNVALTEDGDVWWEGMTDEPPAK
CCEEEECCCCCEEECCCCCCCCCCCCCCEECCCCEEEEEEEEECCCCEEECCCCCCCCCC
LTDWQGNEWTPGCGRLAAHPNSRFTAPASQCPSIDPDWENPKGVPMSAFIFGGRRKDTVP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCE
LVYQAFNWSFGVYLASTLGSETTAAAVGATGNVRRDPFAMLPFCGYHMADYFSHWLQFGR
EEEEECCCCCHHHHHHHCCCCHHHEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHH
SIAKPPRIFSVNWFRKDANGKFIWPGYGDNMRVLKWIVERVQGKAAAVESPLGWMPRYED
HHCCCCCEEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCC
MDWEGLDMTREKFNELTSVDREVWHNELVSHEELFLKLYDRLPKEFLHMRQMIVSGLWRS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEHWEQIGDVHPEGWNE
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA