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The map label for this gene is nuoB [H]

Identifier: 78223793

GI number: 78223793

Start: 2945688

End: 2946422

Strand: Reverse

Name: nuoB [H]

Synonym: Gmet_2596

Alternate gene names: 78223793

Gene position: 2946422-2945688 (Counterclockwise)

Preceding gene: 78223794

Following gene: 78223790

Centisome position: 73.71

GC content: 61.22

Gene sequence:

>735_bases
ATGTTCCGAGCAATCGCTTCCCGAATCAAGCAGGGCCATCGCACCACCCCCTATCCCGCTGCGCCGCTGCAGATGCCTGA
GCGGTTCCGCGGCTATCCGCTTGTCAGCCCGGCAAACGAGGCTTCCGAGGCCACGTGCCCCTACGGTGCTTACTCAAAGG
AGAGCGGCGGGTGTCTCGACATGGGCAAATGTCTTTTCTGTGCCGAATGCCCGGTAGGGACCATCCAGTTTACCACGGAC
TATCAACTGGCCGTTACGAACCGTGACCATCTGCTGGTTCATCCCGGATCGGAACATCACCACGCCCGGCCGCTCCCTCC
CGACCTGCGGCGTCTGTTCGGGCGCTCCCTTAAATTCCGGGAGGTCAGCGCCGGCGGGTGTAACGCCTGCGAAGCCGATA
CCAATGTCCTTTCGACCATCGGCTGGGATCTCGGCCGGTTCGGGATCCAGTTCGTGGCGTCGCCGCGCCACGCGGATGGC
CTGTGGGTAACCGGTCCGGTTACCGAACACATGGCCAAGGCACTTCTGACCACCTATGAGGCGATTCCTGACCCGAAGAT
CGTTGTCGCCTGCGGCGCCTGCGCCATCAACGGCGGACCTTACATCGACACCCCCCAGGTCTGCAATGGCGTGGACAGCA
TTGTTCCCGTTGATCTGTACATCCCCGGTTGCCCACCCCATCCGGCTACCATCCTTGATGGCCTGCTGCGCATTCTGAAT
AAAATAAAGGGATAA

Upstream 100 bases:

>100_bases
CTTGGCGCTTCGGGGACAGCAAATATCCGATTTTCCGCTCTGCAACAAGAGCTTCAACCTGTCCTATTGCGGGTTCGACC
TCTAGGGAGGATAACGTTAC

Downstream 100 bases:

>100_bases
TCACTGACGGGAGTCGAGGAGGGCGAGCGCTCCTTCGACCGTATCCTGGTGGTGCCGCCACTCCCGGGCCGCGTCGAAAC
GGGTCCAGGCGGCGGTGCAG

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MFRAIASRIKQGHRTTPYPAAPLQMPERFRGYPLVSPANEASEATCPYGAYSKESGGCLDMGKCLFCAECPVGTIQFTTD
YQLAVTNRDHLLVHPGSEHHHARPLPPDLRRLFGRSLKFREVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADG
LWVTGPVTEHMAKALLTTYEAIPDPKIVVACGACAINGGPYIDTPQVCNGVDSIVPVDLYIPGCPPHPATILDGLLRILN
KIKG

Sequences:

>Translated_244_residues
MFRAIASRIKQGHRTTPYPAAPLQMPERFRGYPLVSPANEASEATCPYGAYSKESGGCLDMGKCLFCAECPVGTIQFTTD
YQLAVTNRDHLLVHPGSEHHHARPLPPDLRRLFGRSLKFREVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADG
LWVTGPVTEHMAKALLTTYEAIPDPKIVVACGACAINGGPYIDTPQVCNGVDSIVPVDLYIPGCPPHPATILDGLLRILN
KIKG
>Mature_244_residues
MFRAIASRIKQGHRTTPYPAAPLQMPERFRGYPLVSPANEASEATCPYGAYSKESGGCLDMGKCLFCAECPVGTIQFTTD
YQLAVTNRDHLLVHPGSEHHHARPLPPDLRRLFGRSLKFREVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADG
LWVTGPVTEHMAKALLTTYEAIPDPKIVVACGACAINGGPYIDTPQVCNGVDSIVPVDLYIPGCPPHPATILDGLLRILN
KIKG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=118, Percent_Identity=43.2203389830509, Blast_Score=104, Evalue=8e-23,
Organism=Escherichia coli, GI1788834, Length=133, Percent_Identity=47.3684210526316, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=43.1654676258993, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788624, Length=118, Percent_Identity=38.9830508474576, Blast_Score=84, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=118, Percent_Identity=40.6779661016949, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI18859983, Length=118, Percent_Identity=38.9830508474576, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24642371, Length=118, Percent_Identity=38.9830508474576, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24651058, Length=118, Percent_Identity=39.8305084745763, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 26322; Mature: 26322

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRAIASRIKQGHRTTPYPAAPLQMPERFRGYPLVSPANEASEATCPYGAYSKESGGCLD
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
MGKCLFCAECPVGTIQFTTDYQLAVTNRDHLLVHPGSEHHHARPLPPDLRRLFGRSLKFR
CCCEEEEECCCCCEEEEECCEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCEE
EVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADGLWVTGPVTEHMAKALLTTYE
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHHHHHHHHHHHHHH
AIPDPKIVVACGACAINGGPYIDTPQVCNGVDSIVPVDLYIPGCPPHPATILDGLLRILN
HCCCCEEEEEECCEEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
KIKG
HHCC
>Mature Secondary Structure
MFRAIASRIKQGHRTTPYPAAPLQMPERFRGYPLVSPANEASEATCPYGAYSKESGGCLD
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
MGKCLFCAECPVGTIQFTTDYQLAVTNRDHLLVHPGSEHHHARPLPPDLRRLFGRSLKFR
CCCEEEEECCCCCEEEEECCEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCEE
EVSAGGCNACEADTNVLSTIGWDLGRFGIQFVASPRHADGLWVTGPVTEHMAKALLTTYE
EECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHHHHHHHHHHHHHH
AIPDPKIVVACGACAINGGPYIDTPQVCNGVDSIVPVDLYIPGCPPHPATILDGLLRILN
HCCCCEEEEEECCEEECCCCCCCCHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
KIKG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA