Click here to switch to the map view.

The map label for this gene is fumB [H]

Identifier: 78223767

GI number: 78223767

Start: 2917648

End: 2919273

Strand: Direct

Name: fumB [H]

Synonym: Gmet_2570

Alternate gene names: 78223767

Gene position: 2917648-2919273 (Clockwise)

Preceding gene: 78223762

Following gene: 78223768

Centisome position: 72.99

GC content: 62.18

Gene sequence:

>1626_bases
ATGTCCACCAAGCCGTTCGTTTACCAGGAACCCTTCCCCCTCGAGAAAGATGAGACGTCGTACTACAAGATTCCCGACTC
GGAGAAGTATGTCAGCGTCGCAACTTTCGAAGGGAAAGAGGTCCTCAAGGTCGATCCCGAGGCACTCACCGTCCTGGCCA
ACACGGCCATGAGGGACGTCTCCTTCCTGCTCCGCCCCGAGCACAACGAGTCGGTGGCCAAGATCCTCCGGGATCCGGAA
GCCTCCCAGAACGACAAGGGGGTTGCCCTGGCATTCCTGCGGAACGCCGAGATCGCCGCCAACTTCGAGCTCCCCCTCTG
CCAGGACACCGGCACCGCCACCGTCGTGGCCAAGAAAGGGCAGCAGGTTTGGACCGGCGGAAAGGACGAGGAGTTTCTCT
CCAAGGGGGTCTACAAGACCTACACCGAGGAAAATCTCCGCTACTCCCAGACCGTGGCCCTGGACATGTACAAGGAAGTC
AACACCGGCACCAACCTGCCGGCCCAGATCGACATCCAGGCTGTGGACGGCGATTACTACAAGTTCCTCTTCATGGCCAA
AGGGGGGGGCTCCGCCAACAAGACCATGCTCTACCAGGAGACCAAGGCACTCCTTACCCCGGACAGCCTGCTCAAGTTCC
TGGTGGGCAAGATGAAGTACCTGGGAACCGCCGCCTGCCCGCCGTACCACATCGCCGTCGTGGTCGGCGGTACCTCGGCC
GACGCCTGCATGAAGGCGGTCAAGCTCGCCTCCGCCAAGTACCTTGACGCCCTCCCCACCACGGGGAACGAGCACGGCCG
GGCCTTCCGCGACGTGGAACTGGAGGAGAAGCTTCTCCAAGAGGCCTACAAGCTCGGCATCGGCGCCCAGTTCGGCGGCA
AGTACTTCGCCCATGACGTACGGGTCATCCGGCTTCCCCGCCACGGCGCCTCCTGCCCCGTGGGGATGGCGGTCTCCTGT
TCCGCCGACCGCAACATCAAGGCCAAGATCACCCAAAACGGCCTCTTCGTCGAGGAACTGGACCGCAATCCGAGCCGACT
CATTCCCGAGCAGTACCGCGGCAAGCATGAGCACGGGGTCAAGATCGACCTGAACCGCCCCATGAAGGAGATCCTGGCCG
AACTGAGCAAGCACCCGGTTTCCACGCCGCTGCTGCTGAATGGCACCATCGTGGTGGGGCGCGACATCGCCCACGCCAAG
TTCAAAGAGATTCTCGACAGCGGCAAACCGCTCCCCGACTATCTCAAGAATCATCCGATCTACTACGCCGGACCGGCCAA
GACCCCGGCTGGCAAGCCTTCCGGCTCCTTCGGCCCCACCACTGCCGGCCGCATGGACTCCTACGTGGATCTTCTCCAGG
CGAACGGCGGCTCCATGATCATGATCGCCAAGGGGAACCGGAGCCAGCAGGTGACCGATGCCTGCAAGAAGCACGGCGGC
TTCTACCTGGGCTCCATCGGCGGCCCTGCCGCGGTCCTGGCCGAGGAGAACATCAAGAAGGTCGAGTGCATCGACTTCCC
CGAACTGGGGATGGAAGCGGTCTGGAAGATCGAGGTCGAGAACTTCCCGGCCTTCATCCTGGTGGATGACAAGGGGAATG
ACTTCTTCAAGCAGTTGGGTCTGTAG

Upstream 100 bases:

>100_bases
CAACTTCCCTCTCGACAAAATGAAAAATATTACATAAGATTGCGACCTGCATACTGTATACAGAAAACTCCTCATAAACC
CACGCGAAAGGAGAGCATGT

Downstream 100 bases:

>100_bases
TATCCCCCATGAAAGTGGCAAAAGCCCCCGGTCGTATCTCGCGGCCGGGGGCTTTTCCGTGTGCGGAAGGGAATTCTCCC
TACCCGCGCGGGGAGCGCGT

Product: fumarase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSTKPFVYQEPFPLEKDETSYYKIPDSEKYVSVATFEGKEVLKVDPEALTVLANTAMRDVSFLLRPEHNESVAKILRDPE
ASQNDKGVALAFLRNAEIAANFELPLCQDTGTATVVAKKGQQVWTGGKDEEFLSKGVYKTYTEENLRYSQTVALDMYKEV
NTGTNLPAQIDIQAVDGDYYKFLFMAKGGGSANKTMLYQETKALLTPDSLLKFLVGKMKYLGTAACPPYHIAVVVGGTSA
DACMKAVKLASAKYLDALPTTGNEHGRAFRDVELEEKLLQEAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITQNGLFVEELDRNPSRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKHPVSTPLLLNGTIVVGRDIAHAK
FKEILDSGKPLPDYLKNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL

Sequences:

>Translated_541_residues
MSTKPFVYQEPFPLEKDETSYYKIPDSEKYVSVATFEGKEVLKVDPEALTVLANTAMRDVSFLLRPEHNESVAKILRDPE
ASQNDKGVALAFLRNAEIAANFELPLCQDTGTATVVAKKGQQVWTGGKDEEFLSKGVYKTYTEENLRYSQTVALDMYKEV
NTGTNLPAQIDIQAVDGDYYKFLFMAKGGGSANKTMLYQETKALLTPDSLLKFLVGKMKYLGTAACPPYHIAVVVGGTSA
DACMKAVKLASAKYLDALPTTGNEHGRAFRDVELEEKLLQEAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSC
SADRNIKAKITQNGLFVEELDRNPSRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKHPVSTPLLLNGTIVVGRDIAHAK
FKEILDSGKPLPDYLKNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL
>Mature_540_residues
STKPFVYQEPFPLEKDETSYYKIPDSEKYVSVATFEGKEVLKVDPEALTVLANTAMRDVSFLLRPEHNESVAKILRDPEA
SQNDKGVALAFLRNAEIAANFELPLCQDTGTATVVAKKGQQVWTGGKDEEFLSKGVYKTYTEENLRYSQTVALDMYKEVN
TGTNLPAQIDIQAVDGDYYKFLFMAKGGGSANKTMLYQETKALLTPDSLLKFLVGKMKYLGTAACPPYHIAVVVGGTSAD
ACMKAVKLASAKYLDALPTTGNEHGRAFRDVELEEKLLQEAYKLGIGAQFGGKYFAHDVRVIRLPRHGASCPVGMAVSCS
ADRNIKAKITQNGLFVEELDRNPSRLIPEQYRGKHEHGVKIDLNRPMKEILAELSKHPVSTPLLLNGTIVVGRDIAHAKF
KEILDSGKPLPDYLKNHPIYYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLGL

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=540, Percent_Identity=74.0740740740741, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=542, Percent_Identity=73.2472324723247, Blast_Score=828, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=29.2817679558011, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789442, Length=260, Percent_Identity=27.3076923076923, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59307; Mature: 59175

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPFVYQEPFPLEKDETSYYKIPDSEKYVSVATFEGKEVLKVDPEALTVLANTAMRDV
CCCCCEEECCCCCCCCCCCCEEECCCCCCEEEEEEECCCEEEEECHHHHHHHHHHHHHHH
SFLLRPEHNESVAKILRDPEASQNDKGVALAFLRNAEIAANFELPLCQDTGTATVVAKKG
HHEECCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCEEECCCCCEEEEEECC
QQVWTGGKDEEFLSKGVYKTYTEENLRYSQTVALDMYKEVNTGTNLPAQIDIQAVDGDYY
CEEECCCCCHHHHHCCCHHHHCCCCCEEHHEEEEHHHHHHCCCCCCCEEEEEEEECCCEE
KFLFMAKGGGSANKTMLYQETKALLTPDSLLKFLVGKMKYLGTAACPPYHIAVVVGGTSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DACMKAVKLASAKYLDALPTTGNEHGRAFRDVELEEKLLQEAYKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITQNGLFVEELDRNPSRLIPEQYRGKHEHGV
EEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHCCCCHHCCCHHHCCCCCCCE
KIDLNRPMKEILAELSKHPVSTPLLLNGTIVVGRDIAHAKFKEILDSGKPLPDYLKNHPI
EEECCCCHHHHHHHHHCCCCCCCEEEECEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C
>Mature Secondary Structure 
STKPFVYQEPFPLEKDETSYYKIPDSEKYVSVATFEGKEVLKVDPEALTVLANTAMRDV
CCCCEEECCCCCCCCCCCCEEECCCCCCEEEEEEECCCEEEEECHHHHHHHHHHHHHHH
SFLLRPEHNESVAKILRDPEASQNDKGVALAFLRNAEIAANFELPLCQDTGTATVVAKKG
HHEECCCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEECCCEEECCCCCEEEEEECC
QQVWTGGKDEEFLSKGVYKTYTEENLRYSQTVALDMYKEVNTGTNLPAQIDIQAVDGDYY
CEEECCCCCHHHHHCCCHHHHCCCCCEEHHEEEEHHHHHHCCCCCCCEEEEEEEECCCEE
KFLFMAKGGGSANKTMLYQETKALLTPDSLLKFLVGKMKYLGTAACPPYHIAVVVGGTSA
EEEEEECCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCH
DACMKAVKLASAKYLDALPTTGNEHGRAFRDVELEEKLLQEAYKLGIGAQFGGKYFAHDV
HHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCCEEECCE
RVIRLPRHGASCPVGMAVSCSADRNIKAKITQNGLFVEELDRNPSRLIPEQYRGKHEHGV
EEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCHHHHCCCCHHCCCHHHCCCCCCCE
KIDLNRPMKEILAELSKHPVSTPLLLNGTIVVGRDIAHAKFKEILDSGKPLPDYLKNHPI
EEECCCCHHHHHHHHHCCCCCCCEEEECEEEEECCHHHHHHHHHHHCCCCCHHHHHCCCE
YYAGPAKTPAGKPSGSFGPTTAGRMDSYVDLLQANGGSMIMIAKGNRSQQVTDACKKHGG
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCC
FYLGSIGGPAAVLAEENIKKVECIDFPELGMEAVWKIEVENFPAFILVDDKGNDFFKQLG
EEEECCCCCEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]