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The map label for this gene is rne [H]

Identifier: 78223743

GI number: 78223743

Start: 2890391

End: 2892811

Strand: Direct

Name: rne [H]

Synonym: Gmet_2546

Alternate gene names: 78223743

Gene position: 2890391-2892811 (Clockwise)

Preceding gene: 78223733

Following gene: 78223744

Centisome position: 72.31

GC content: 64.02

Gene sequence:

>2421_bases
ATGGCAAAAAAGATGCTCATCAACGTGATGCATCCGGAGGAGGCCCGCGTTGCCATCGTCGAAGACGGCAAGCTGGACAA
TCTGGACATCGAGATCGCGGGGAGCGAACAGACCCGCGGCAACGTCTACAAGGGGGTCGTGGTCCGGGTGGAGCCGGGGC
TCCAGGCGGCCTTCGTGGATATCGGGGCCAAAAGGCTCGGCTTCCTCCAGATGGGGGAGATTCACCCCTCCTTCTGGCAG
TGGCGCGACGACGTCCCCCCCGAGAACCGCAACCGCCGCCCCCGCATCCAGGAAGTGATCCGCCGGGGCCAGGAACTCAT
CGTCCAAGTGGAAAAGGGTGAACGGGACATGAAGGGGGCGGCCCTCACCACGTACATGTCGTTCCCGGGGCGCTACATGG
TCCTTATGCCGGGAAGCGACTCCGCCGGCATCTCCCGCAAGGTGGAGAGCGATGCCGACCGCAAGAAACTGAAGGAGAAG
CTCAGCCAGATGGAGATCCCCGAGGGGATCGGCTATATCGTCCGGACCGAGGCCCTCGGCAAGACCAAGACCGAGCTGAA
CAAGGACCTCCAGAACCTCCTCACTCTTTACAAGTCGATCCAGGAAAAGGCGGCCGCCCTCAAGGCCCCGGCCCTCATCC
ACCAGGAGATGAACGTGGTCATCCGGACCATCCGCGACTACTTCACCGCCGAGATCGACGAAGTGCTCGTGGACAGCAAG
GAGGTCTACAAGGAGGCCCGGGACTTCTTCAAGCAGACCATGCCCAAATACGAGAAACTGGTGAAGCTCCACACGGAGAA
GCGCCCCATCTTCTCCCGCTACCAGATTGAGGAGCAGATCGACCTCATCTACGAGAAGAAGGTCCCTCTAAAATCCGGCG
GCTACCTCATCATCGAGCCCACCGAGGCCCTGGTCTCCATCGACGTCAACTCCGGCAAGACCACCGGGGAGAAGGGGGTG
GAGGATACCGCCTACAAGACCAATTTGGAGGCGGCGGAAGAGGCGGCCCGGCAACTGCGGCTGCGGGACCTGGGGGGGCT
CATCGTCCTTGATTTCATCGACATGCGGGACCGCAAGCACAACGCCACCGTGGAGAAGACTCTCAAGGCCGCCCTCAAGG
AGGACAAGGCCCGGGTCGAGGTGGGGCGCATCTCTCAGTTCGGCATGCTGGAGATGTCCCGCCAGCGGATCAAGCAGACC
CTTGAGCAGGGAAGCACCCTCGAATGCCCCCACTGCAGCGGCCGGGGGAAGGTGAAGAGCGTTGAGAGCATGGCCCTCTC
CTTCCTCCGCAAGGTCCACGCCGCCGCAGCCAAGGGAACCGTGGCCGAGGTTCATGGCGGCCTCCCCCTGGAGGTGGCCT
ACTACCTCCTGAACCGCAAGAAGCGAGAACTGGCCCGGATCGAGGACGACTACGACATCGTGGTGACGGTCAAGGGGCGG
ACTTCGTTCCTCATGAACGAGCTGGAACTGGAGACGGTCAAGCGGGAAAAGCCGGCTCACATCGAGCATCCGGCCGAAGC
GGCGGAGGCGCCGGAAAAGAAGCCGGAAACCGTCATGGAAGCCGCCGAGGCCGTGGAACCCGAGGAGACGGCTCTCCAGG
AAGGCGGAGAAGGGAAAAAGCGCAAGCGTCGCCGCAGCCGCAAGAAAGGAAAGGGAGAGGAAACTCCGGCCGTCTCCCTT
GCCGAAGAGCCTTTGCAGGAGGAAGAAGTCGTCGGGGAGCCTGAAGCCGGTGAGGAACCGGAGGAGATCGAAGAGGGGGT
CGAGGCTGCGCCGGGTGAAGAAGCCAAGAAAAAGCGGCGCAGGAGACGGCGCCGCGGGAAGAAGCCGCGCGCCGGCGAAG
CCGGCGAGGTTCCGGCCGCTGAAGTCACCGCTGAGATTGTGACTCCCGAGCCGGCCGTCGAACCCTCCGCGCCGATTGAT
GCGGAATCCGCAGCGGAAGCAGAGGAACCAGCAGAACCGGCTGCAGAGGAGGCGAAAAAGAAGCGCCGCCGCCGCAAGCG
CCGCAGCGGCAAGCGAGAGGAGGAGGGACTGGCCGCCGAAGAGGCAGCCACCCCGCAAGAAACAGCCCCAGTGGCGCCGG
AGCCTGCGCCTGCATCGGCTGTCGAGCCTGCACCGGAAGAGCCGAAGCCGAAGAAGACCCGTGCACCGCGTACCAAAAGG
GCTGCTGTTGCGGCTTCAGAACCCGCGGAGCCACAGACCGTCGAGGCGGTATCCGCCGAACCGGCACCTACTGAAGCGGC
ACCGGCAAAGCCTGTGCGCAAACGGGCACCCCGCAAGAAAGCCGTCGCGGAGGAGGAAGCCATCGCCCCTGAGGCGCACG
CGCCGGAGCCGACGCCGGAAGCCGAGGCACCGAAACGCAAGCGGGCACCGCGCAAGAAGAAGGAAACCCCTGAAGCTCCG
CCTGAGAATGATACTCCATGA

Upstream 100 bases:

>100_bases
CTTTGAATACACATCAATCGGGCTGCCCGGCTCCGTGCCTGGGGCGGCCGACATTTCAGCGGCGCGGGGTGCGCCCCGCC
GCCCAAAAGGACAGGAACCT

Downstream 100 bases:

>100_bases
CGAACGGACGGTGAGGAGGAACGATCATGATCAACCACATCGCGATAACGGCGCTTGCCGCCCTTCTGATGGTCTCCCCC
TGCCTCGCAGCGGGTCTTGA

Product: ribonuclease E

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MAKKMLINVMHPEEARVAIVEDGKLDNLDIEIAGSEQTRGNVYKGVVVRVEPGLQAAFVDIGAKRLGFLQMGEIHPSFWQ
WRDDVPPENRNRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESDADRKKLKEK
LSQMEIPEGIGYIVRTEALGKTKTELNKDLQNLLTLYKSIQEKAAALKAPALIHQEMNVVIRTIRDYFTAEIDEVLVDSK
EVYKEARDFFKQTMPKYEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGV
EDTAYKTNLEAAEEAARQLRLRDLGGLIVLDFIDMRDRKHNATVEKTLKAALKEDKARVEVGRISQFGMLEMSRQRIKQT
LEQGSTLECPHCSGRGKVKSVESMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELARIEDDYDIVVTVKGR
TSFLMNELELETVKREKPAHIEHPAEAAEAPEKKPETVMEAAEAVEPEETALQEGGEGKKRKRRRSRKKGKGEETPAVSL
AEEPLQEEEVVGEPEAGEEPEEIEEGVEAAPGEEAKKKRRRRRRRGKKPRAGEAGEVPAAEVTAEIVTPEPAVEPSAPID
AESAAEAEEPAEPAAEEAKKKRRRRKRRSGKREEEGLAAEEAATPQETAPVAPEPAPASAVEPAPEEPKPKKTRAPRTKR
AAVAASEPAEPQTVEAVSAEPAPTEAAPAKPVRKRAPRKKAVAEEEAIAPEAHAPEPTPEAEAPKRKRAPRKKKETPEAP
PENDTP

Sequences:

>Translated_806_residues
MAKKMLINVMHPEEARVAIVEDGKLDNLDIEIAGSEQTRGNVYKGVVVRVEPGLQAAFVDIGAKRLGFLQMGEIHPSFWQ
WRDDVPPENRNRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESDADRKKLKEK
LSQMEIPEGIGYIVRTEALGKTKTELNKDLQNLLTLYKSIQEKAAALKAPALIHQEMNVVIRTIRDYFTAEIDEVLVDSK
EVYKEARDFFKQTMPKYEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGV
EDTAYKTNLEAAEEAARQLRLRDLGGLIVLDFIDMRDRKHNATVEKTLKAALKEDKARVEVGRISQFGMLEMSRQRIKQT
LEQGSTLECPHCSGRGKVKSVESMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELARIEDDYDIVVTVKGR
TSFLMNELELETVKREKPAHIEHPAEAAEAPEKKPETVMEAAEAVEPEETALQEGGEGKKRKRRRSRKKGKGEETPAVSL
AEEPLQEEEVVGEPEAGEEPEEIEEGVEAAPGEEAKKKRRRRRRRGKKPRAGEAGEVPAAEVTAEIVTPEPAVEPSAPID
AESAAEAEEPAEPAAEEAKKKRRRRKRRSGKREEEGLAAEEAATPQETAPVAPEPAPASAVEPAPEEPKPKKTRAPRTKR
AAVAASEPAEPQTVEAVSAEPAPTEAAPAKPVRKRAPRKKAVAEEEAIAPEAHAPEPTPEAEAPKRKRAPRKKKETPEAP
PENDTP
>Mature_805_residues
AKKMLINVMHPEEARVAIVEDGKLDNLDIEIAGSEQTRGNVYKGVVVRVEPGLQAAFVDIGAKRLGFLQMGEIHPSFWQW
RDDVPPENRNRRPRIQEVIRRGQELIVQVEKGERDMKGAALTTYMSFPGRYMVLMPGSDSAGISRKVESDADRKKLKEKL
SQMEIPEGIGYIVRTEALGKTKTELNKDLQNLLTLYKSIQEKAAALKAPALIHQEMNVVIRTIRDYFTAEIDEVLVDSKE
VYKEARDFFKQTMPKYEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEPTEALVSIDVNSGKTTGEKGVE
DTAYKTNLEAAEEAARQLRLRDLGGLIVLDFIDMRDRKHNATVEKTLKAALKEDKARVEVGRISQFGMLEMSRQRIKQTL
EQGSTLECPHCSGRGKVKSVESMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELARIEDDYDIVVTVKGRT
SFLMNELELETVKREKPAHIEHPAEAAEAPEKKPETVMEAAEAVEPEETALQEGGEGKKRKRRRSRKKGKGEETPAVSLA
EEPLQEEEVVGEPEAGEEPEEIEEGVEAAPGEEAKKKRRRRRRRGKKPRAGEAGEVPAAEVTAEIVTPEPAVEPSAPIDA
ESAAEAEEPAEPAAEEAKKKRRRRKRRSGKREEEGLAAEEAATPQETAPVAPEPAPASAVEPAPEEPKPKKTRAPRTKRA
AVAASEPAEPQTVEAVSAEPAPTEAAPAKPVRKRAPRKKAVAEEEAIAPEAHAPEPTPEAEAPKRKRAPRKKKETPEAPP
ENDTP

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=479, Percent_Identity=47.5991649269311, Blast_Score=444, Evalue=1e-125,
Organism=Escherichia coli, GI87082235, Length=440, Percent_Identity=38.1818181818182, Blast_Score=291, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 89523; Mature: 89392

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKMLINVMHPEEARVAIVEDGKLDNLDIEIAGSEQTRGNVYKGVVVRVEPGLQAAFVD
CCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCHHHHHH
IGAKRLGFLQMGEIHPSFWQWRDDVPPENRNRRPRIQEVIRRGQELIVQVEKGERDMKGA
HHHHHHCCHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCCHHEEEEECCCCCCCCCH
ALTTYMSFPGRYMVLMPGSDSAGISRKVESDADRKKLKEKLSQMEIPEGIGYIVRTEALG
HHHHHCCCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHC
KTKTELNKDLQNLLTLYKSIQEKAAALKAPALIHQEMNVVIRTIRDYFTAEIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EVYKEARDFFKQTMPKYEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKTTGEKGVEDTAYKTNLEAAEEAARQLRLRDLGGLIVLDFIDMRDRKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCC
NATVEKTLKAALKEDKARVEVGRISQFGMLEMSRQRIKQTLEQGSTLECPHCSGRGKVKS
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VESMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELARIEDDYDIVVTVKGR
HHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
TSFLMNELELETVKREKPAHIEHPAEAAEAPEKKPETVMEAAEAVEPEETALQEGGEGKK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHH
RKRRRSRKKGKGEETPAVSLAEEPLQEEEVVGEPEAGEEPEEIEEGVEAAPGEEAKKKRR
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
RRRRRGKKPRAGEAGEVPAAEVTAEIVTPEPAVEPSAPIDAESAAEAEEPAEPAAEEAKK
HHHHCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHH
KRRRRKRRSGKREEEGLAAEEAATPQETAPVAPEPAPASAVEPAPEEPKPKKTRAPRTKR
HHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AAVAASEPAEPQTVEAVSAEPAPTEAAPAKPVRKRAPRKKAVAEEEAIAPEAHAPEPTPE
HHCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
AEAPKRKRAPRKKKETPEAPPENDTP
CCCCHHHCCCCHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AKKMLINVMHPEEARVAIVEDGKLDNLDIEIAGSEQTRGNVYKGVVVRVEPGLQAAFVD
CCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCHHHHHH
IGAKRLGFLQMGEIHPSFWQWRDDVPPENRNRRPRIQEVIRRGQELIVQVEKGERDMKGA
HHHHHHCCHHCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHCCHHEEEEECCCCCCCCCH
ALTTYMSFPGRYMVLMPGSDSAGISRKVESDADRKKLKEKLSQMEIPEGIGYIVRTEALG
HHHHHCCCCCCEEEEECCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHC
KTKTELNKDLQNLLTLYKSIQEKAAALKAPALIHQEMNVVIRTIRDYFTAEIDEVLVDSK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
EVYKEARDFFKQTMPKYEKLVKLHTEKRPIFSRYQIEEQIDLIYEKKVPLKSGGYLIIEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
TEALVSIDVNSGKTTGEKGVEDTAYKTNLEAAEEAARQLRLRDLGGLIVLDFIDMRDRKH
CCEEEEEECCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCC
NATVEKTLKAALKEDKARVEVGRISQFGMLEMSRQRIKQTLEQGSTLECPHCSGRGKVKS
CHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
VESMALSFLRKVHAAAAKGTVAEVHGGLPLEVAYYLLNRKKRELARIEDDYDIVVTVKGR
HHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
TSFLMNELELETVKREKPAHIEHPAEAAEAPEKKPETVMEAAEAVEPEETALQEGGEGKK
HHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCHH
RKRRRSRKKGKGEETPAVSLAEEPLQEEEVVGEPEAGEEPEEIEEGVEAAPGEEAKKKRR
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
RRRRRGKKPRAGEAGEVPAAEVTAEIVTPEPAVEPSAPIDAESAAEAEEPAEPAAEEAKK
HHHHCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHH
KRRRRKRRSGKREEEGLAAEEAATPQETAPVAPEPAPASAVEPAPEEPKPKKTRAPRTKR
HHHHHHHHCCCCHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
AAVAASEPAEPQTVEAVSAEPAPTEAAPAKPVRKRAPRKKAVAEEEAIAPEAHAPEPTPE
HHCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
AEAPKRKRAPRKKKETPEAPPENDTP
CCCCHHHCCCCHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]