Click here to switch to the map view.

The map label for this gene is tolC [H]

Identifier: 78223716

GI number: 78223716

Start: 2856706

End: 2858073

Strand: Reverse

Name: tolC [H]

Synonym: Gmet_2517

Alternate gene names: 78223716

Gene position: 2858073-2856706 (Counterclockwise)

Preceding gene: 78223717

Following gene: 78223714

Centisome position: 71.5

GC content: 61.99

Gene sequence:

>1368_bases
ATGACTGACCAACGCTTTGATGATACCGGGAAGAGTGGCATTTACAGGGCGCTCGAGAATAAATTGAGGATTTTCTCCGT
TGCTCCGTTGCTCTGTGTTATTTGGCTTTTGCATGGTATCAGTATGGCCGCCGAGCATCGTGTCCTCACCCTCGATCAGG
CCCTGGAGGTGGCTGCGGAGCGTAACCGCGACATCCTCAAGGCAAAGGAATTCCACAACCAGGTGAGGGGGCGCTATGTG
GAGGAACGGTCTGCTGCCCTTCCCCATCTGACGATCACCGGACAGGCTGTCACCCAGCACGACGAGAGCCTCAATGCCTA
TGCGGACGGATTCATGCCGGTGCGCCAGGATATCTATGGCGCCGAACTCGGTCTCTCTCAGGCTCTCTACACCTGGGGGA
AGGTGGGGGCTGCAATCCGGGCGGCAGAGAAGGGGTTTGCCACCGCCGACGAGCAGTTTCGCAGGGCGCGGCAGGATGCC
TGGCGCGATGTGTCGGTGGCCTTTTACAATATCCTTCTTGCCAGGGAGCAGCACGCCATTGCTTCCCAGAATCTTGCGCA
GAAGGTGCGGCACCAGGATGAGGCCCAGCGCAGGTATGCGGCCGGCGTGGCCACCGATTACGACGTCCTGGCAGCGGATG
TTGCGGTGCAAAATGCCATGCCCGACGTGATCCGGGCCGGCAACAGTGTCCGCGTGGCCCGGGACCGGCTCTCCTTCCTC
CTGGCCCTGGAGGGTGAGGTGGATGTGACCGGCTCGCTGGAGACTGTGCTGACTACCTATCCCTCCTACGACAAAGCCCT
CGCAGTGGCCCGGGATAAACGTCCCGAGCTGAAGGAGATCAAGCATCGCCTCGCCATTGCCGGTGAACTGGTGAAGGTGG
CTGACGCCGACGACAAGCCGCGTCTGGACCTGACGGGGAAATACGGATGGCGGCACCTGGAGGTGGGGGACGGTTCCGGG
AGCGGCCAGATATGGACCGTGGGCCTTCAGTTGAGTTTTCCCCTGTTCGATGGCCTCAAGACCCGGGGCAAGGTGGCCCA
GGCCGAGAGCGAACGGCGAAGCATGCAGATCGACGAGGCTAAACTCCTGGAGTCGGTTGCCCTGGAAATCCGCGATGCGG
TCAATGCTGTTCGCGAGTCCGAGGAGATTGTCAAGGCCCTGTCCGGTACGGTGGTCCAGGCGGAGCGCCTTCTGCAGATG
GCCGAAAAGGGATTCGAGCTGGGGGTCAAGATTCGGCTGGAGGTGGACGACGCCGAACTGAACCTGCGTCAGGCCCGGGG
GAACCTGGCCCGGGCACGGCGCGACTATCTCGTGGCCCGGGTTAATCTGGAGCGGGTCATGGGGGTGCTGGGGGAGGGAA
GTCTCTGA

Upstream 100 bases:

>100_bases
TCGTGCCGGTCATGTACACCTACATGGACGACCTGGCGCTGTGGCTCAGGCGAAAGTGGCGGGGTGAGGAGCAACGGAGT
AACGGAGGGACAGGCATTTC

Downstream 100 bases:

>100_bases
ACGTTTGAGAGCCGGTGTTTCTTTTGTGTTTCCGATTTGAAATTATCTTGAACTGTGCTTTAATGATATCAGGACAGCTG
GTTAGCCTTCACCTCAAAGG

Product: Outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTDQRFDDTGKSGIYRALENKLRIFSVAPLLCVIWLLHGISMAAEHRVLTLDQALEVAAERNRDILKAKEFHNQVRGRYV
EERSAALPHLTITGQAVTQHDESLNAYADGFMPVRQDIYGAELGLSQALYTWGKVGAAIRAAEKGFATADEQFRRARQDA
WRDVSVAFYNILLAREQHAIASQNLAQKVRHQDEAQRRYAAGVATDYDVLAADVAVQNAMPDVIRAGNSVRVARDRLSFL
LALEGEVDVTGSLETVLTTYPSYDKALAVARDKRPELKEIKHRLAIAGELVKVADADDKPRLDLTGKYGWRHLEVGDGSG
SGQIWTVGLQLSFPLFDGLKTRGKVAQAESERRSMQIDEAKLLESVALEIRDAVNAVRESEEIVKALSGTVVQAERLLQM
AEKGFELGVKIRLEVDDAELNLRQARGNLARARRDYLVARVNLERVMGVLGEGSL

Sequences:

>Translated_455_residues
MTDQRFDDTGKSGIYRALENKLRIFSVAPLLCVIWLLHGISMAAEHRVLTLDQALEVAAERNRDILKAKEFHNQVRGRYV
EERSAALPHLTITGQAVTQHDESLNAYADGFMPVRQDIYGAELGLSQALYTWGKVGAAIRAAEKGFATADEQFRRARQDA
WRDVSVAFYNILLAREQHAIASQNLAQKVRHQDEAQRRYAAGVATDYDVLAADVAVQNAMPDVIRAGNSVRVARDRLSFL
LALEGEVDVTGSLETVLTTYPSYDKALAVARDKRPELKEIKHRLAIAGELVKVADADDKPRLDLTGKYGWRHLEVGDGSG
SGQIWTVGLQLSFPLFDGLKTRGKVAQAESERRSMQIDEAKLLESVALEIRDAVNAVRESEEIVKALSGTVVQAERLLQM
AEKGFELGVKIRLEVDDAELNLRQARGNLARARRDYLVARVNLERVMGVLGEGSL
>Mature_454_residues
TDQRFDDTGKSGIYRALENKLRIFSVAPLLCVIWLLHGISMAAEHRVLTLDQALEVAAERNRDILKAKEFHNQVRGRYVE
ERSAALPHLTITGQAVTQHDESLNAYADGFMPVRQDIYGAELGLSQALYTWGKVGAAIRAAEKGFATADEQFRRARQDAW
RDVSVAFYNILLAREQHAIASQNLAQKVRHQDEAQRRYAAGVATDYDVLAADVAVQNAMPDVIRAGNSVRVARDRLSFLL
ALEGEVDVTGSLETVLTTYPSYDKALAVARDKRPELKEIKHRLAIAGELVKVADADDKPRLDLTGKYGWRHLEVGDGSGS
GQIWTVGLQLSFPLFDGLKTRGKVAQAESERRSMQIDEAKLLESVALEIRDAVNAVRESEEIVKALSGTVVQAERLLQMA
EKGFELGVKIRLEVDDAELNLRQARGNLARARRDYLVARVNLERVMGVLGEGSL

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=397, Percent_Identity=25.9445843828715, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50482; Mature: 50351

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQRFDDTGKSGIYRALENKLRIFSVAPLLCVIWLLHGISMAAEHRVLTLDQALEVAAE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
RNRDILKAKEFHNQVRGRYVEERSAALPHLTITGQAVTQHDESLNAYADGFMPVRQDIYG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHCCCHHHHHHHHH
AELGLSQALYTWGKVGAAIRAAEKGFATADEQFRRARQDAWRDVSVAFYNILLAREQHAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASQNLAQKVRHQDEAQRRYAAGVATDYDVLAADVAVQNAMPDVIRAGNSVRVARDRLSFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHEE
LALEGEVDVTGSLETVLTTYPSYDKALAVARDKRPELKEIKHRLAIAGELVKVADADDKP
EEECCCEECCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCC
RLDLTGKYGWRHLEVGDGSGSGQIWTVGLQLSFPLFDGLKTRGKVAQAESERRSMQIDEA
CEEEECCCCEEEEEECCCCCCCCEEEEEEEEECCHHHCHHCCCCHHHHHHHHHHCCCHHH
KLLESVALEIRDAVNAVRESEEIVKALSGTVVQAERLLQMAEKGFELGVKIRLEVDDAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHH
NLRQARGNLARARRDYLVARVNLERVMGVLGEGSL
HHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCC
>Mature Secondary Structure 
TDQRFDDTGKSGIYRALENKLRIFSVAPLLCVIWLLHGISMAAEHRVLTLDQALEVAAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
RNRDILKAKEFHNQVRGRYVEERSAALPHLTITGQAVTQHDESLNAYADGFMPVRQDIYG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHCCCHHHHHHHHH
AELGLSQALYTWGKVGAAIRAAEKGFATADEQFRRARQDAWRDVSVAFYNILLAREQHAI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASQNLAQKVRHQDEAQRRYAAGVATDYDVLAADVAVQNAMPDVIRAGNSVRVARDRLSFL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHEE
LALEGEVDVTGSLETVLTTYPSYDKALAVARDKRPELKEIKHRLAIAGELVKVADADDKP
EEECCCEECCCCHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCC
RLDLTGKYGWRHLEVGDGSGSGQIWTVGLQLSFPLFDGLKTRGKVAQAESERRSMQIDEA
CEEEECCCCEEEEEECCCCCCCCEEEEEEEEECCHHHCHHCCCCHHHHHHHHHHCCCHHH
KLLESVALEIRDAVNAVRESEEIVKALSGTVVQAERLLQMAEKGFELGVKIRLEVDDAEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCEEEEEEECCCHH
NLRQARGNLARARRDYLVARVNLERVMGVLGEGSL
HHHHHHHHHHHHHHHHHHHEECHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]